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Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Genet. | doi: 10.3389/fgene.2019.00587

Human-derived Corneal Epithelial Cells Expressing Cell Cycle Regulators as a New Resource for in vitro Ocular Toxicity Testing

 Tomokazu T. Fukuda1*, Ryo Gouko1, Takahiro Eitsuka2, Ryusei Suzuki1, Kouhei Takahashi1, Kiyotaka Nakagawa2, Eriko Sugano1, Hiroshi Tomita1 and  Tohru Kiyono3
  • 1Iwate University, Japan
  • 2Tohoku University, Japan
  • 3National Cancer Center Research Institute (Japan), Japan

The Draize test has been used on rabbits since the 1960s to evaluate the irritation caused by commercial chemicals in products such as cosmetics or hairdressings. However, since 2003, such tests, including the Draize test for cosmetics, have been prohibited in European countries because they are considered problematic to animal welfare. For this reason, replacement of in vivo methods with the alternative in vitro methods has become an important goal. In this study, we established a corneal epithelial cell line co-expressing a mutant cyclin dependent kinase 4 (CDK4), Cyclin D1, and telomerase reverse transcriptase (TERT). The established cell line maintained its original morphology and had an enhanced proliferation rate. Furthermore, the cells showed a significant, dose-dependent, decrease in viability in an irritation test using glycolic acid and Benzalkonium chloride. These cells can now be shared with toxicology scientists and should contribute to increasing the reproducibility of chemical testing in vitro.

Keywords: Draize eye irritation test, cell culture, Toxicology in vitro, Cell Cycle, Irritation and sensitization

Received: 13 Sep 2018; Accepted: 04 Jun 2019.

Edited by:

Pradyumna K. Mishra, ICMR-National Institute for Research in Environmental Health, India

Reviewed by:

Raj K. Koiri, Department of Zoology, Dr. Harisingh Gour Central University, India
Gyanendra Singh, National Institute of Occupational Health (ICMR), India  

Copyright: © 2019 Fukuda, Gouko, Eitsuka, Suzuki, Takahashi, Nakagawa, Sugano, Tomita and Kiyono. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Prof. Tomokazu T. Fukuda, Iwate University, Morioka, 020-8550, Iwate, Japan,