Original Research ARTICLE
Single-cell transcriptomics reveals spatial and temporal turnover of keratinocyte differentiation regulators
- 1University of Illinois at Urbana-Champaign, United States
- 2University of California, San Francisco, United States
- 3Institute for Human Genetics, University of Illinois at Urbana-Champaign, United States
Keratinocyte differentiation requires intricately coordinated spatiotemporal expression changes that specify epidermis structure and function. This paper utilizes single-cell RNA-seq data from 22,338 human foreskin keratinocytes to reconstruct the transcriptional regulation of skin development and homeostasis genes, organizing them by differentiation stage and also into transcription factor (TF)-associated modules. We identify groups of TFs characterized by coordinate expression changes during progression from the undifferentiated basal to the differentiated state and show that these TFs also have concordant differential predicted binding enrichment in the super-enhancers previously reported to turn over between the two states. The identified TFs form a core subset of the regulators controlling gene modules essential for basal and differentiated keratinocyte functions, supporting their nomination as master coordinators of keratinocyte differentiation. Experimental depletion of the TFs ZBED2 and ETV4, both predicted to promote the basal state, induces differentiation. Furthermore, our single-cell RNA expression analysis reveals preferential expression of antioxidant genes in the basal state, suggesting keratinocytes actively suppress reactive oxygen species to maintain the undifferentiated state. Overall, our work demonstrates diverse computational methods to advance our understanding of dynamic gene regulation in development.
Keywords: keratinocyte, differentiation, single-cell, network, antioxidant
Received: 17 Apr 2019;
Accepted: 23 Jul 2019.
Copyright: © 2019 Finnegan, Cho, Luu, Harirchian, Lee, Cheng and Song. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Prof. Jun S. Song, University of Illinois at Urbana-Champaign, Institute for Human Genetics, Champaign, 94143-0794, CA, United States, firstname.lastname@example.org