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Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Genet. | doi: 10.3389/fgene.2019.00794

Effects of agricultural pesticides in aquafeeds on wild fish feeding on leftover pellets near fish farms

 Pål A. Olsvik1*,  Anett K. Larsen2, Marc H. Berntssen3, Anders Goksøyr4, Odd A. Karlsen4,  Fekadu Yadetie4, Monica Sanden3 and  Torstein Kristensen1
  • 1Nord University, Norway
  • 2UiT The Arctic University of Norway, Norway
  • 3Norwegian Institute of Marine Research (IMR), Norway
  • 4University of Bergen, Norway

Screening has revealed that modern-day feeds used in Atlantic salmon aquaculture might contain trace amounts of agricultural pesticides. To reach slaughter size, salmon are produced in open net pens in the sea. Uneaten feed pellets and undigested feces deposited beneath the net pens represent a source of contamination for marine organisms. To examine the impacts of long-term and continuous dietary exposure to an organophosphorus pesticide (OP) found in Atlantic salmon feed, we fed juvenile Atlantic cod (Gadus morhua), an abundant species around North Atlantic fish farms, three concentrations (0.5, 4.2 and 23.2 mg/kg) of chlorpyrifos-methyl (CPM) for 30 days. Endpoints included liver and bile bioaccumulation, liver transcriptomics and metabolomics, as well as plasma cholinesterase activity, cortisol, liver 7-ethoxyresor-ufin-O-deethylase (EROD) activity and hypoxia tolerance. The results show that Atlantic cod can accumulate relatively high levels of CPM in liver after continuous exposure, which is then metabolized and excreted via the bile. All three exposure concentrations lead to significant inhibition of plasma cholinesterase activity, the primary target of CPM. Transcriptomics profiling pointed to effects on cholesterol and steroid biosynthesis. Metabolite profiling revealed that CPM induced responses reflecting detoxification by glutathione-S-transferase, inhibition of monoacylglycerol lipase, potential inhibition of carboxylesterase, and increased demand for ATP, followed by secondary inflammatory responses. A gradual hypoxia challenge test showed that all groups of exposed fish were less tolerant to low oxygen saturation than the controls. In conclusion, this study suggests that wild fish continuously feeding on leftover pellets near fish farms over time may be vulnerable to OPs.

Keywords: Aquaculture, Fish feed, Insecticides, Chlorpyrifos-methyl, Wild fish exposure, Metabolomics, Transcriptomics

Received: 24 Apr 2019; Accepted: 29 Jul 2019.

Edited by:

Christopher J. Martyniuk, University of Florida, United States

Reviewed by:

Mengqing Liang, Yellow Sea Fisheries Research Institute (CAFS), China
Ana Teresa Gonçalves, Universidad de Concepción, Chile  

Copyright: © 2019 Olsvik, Larsen, Berntssen, Goksøyr, Karlsen, Yadetie, Sanden and Kristensen. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Prof. Pål A. Olsvik, Nord University, Bodø, Norway, pal.a.olsvik@nord.no