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Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Genet. | doi: 10.3389/fgene.2019.01019

Identification of a novel SBP1-containing SCFcomplex in wild dwarf almond (Prunus tenella)

 Bin Zeng1*, Jianyou Wang2,  Qing Hao3, Zhenfan Yu1, Ayimaiti Abudukayoumu1, Yilian Tang1, Xiangfei Zhang1 and Xinxin Ma1
  • 1College of Forestry and Horticulture, Xinjiang Agricultural University, China
  • 2Chinese Academy of Forestry, Xinjiang Branch, China
  • 3Horticultural Crop Research Institute, Xinjiang Academy of Agricultural Sciences, China

The S-RNase-based gametophytic self-incompatibility (SI), in which pistil and pollen specificities are determined by S-RNase and the S locus F-box protein respectively, has been found in the Solanaceae, Plantaginaceae, and Rosaceae families, but some underlying molecular mechanism remained elusive and controversial. Previous studies have discovered SI in wild dwarf almond (Prunus tenella), and pistil S (S-RNase) and pollen S (SFB) determinant genes have been investigated. However, the SCF (SKP1–Cullin1–F-box-Rbx1) complex, which functions as an E3 ubiquitin ligase on non-self S-RNase, has not been investigated. In current study, the PetSSK1 (SLF-interacting-SKP1-like1), SBP1(S-RNase binding protein 1), CUL1, SFBs (S-haplotype-specific F-box) were identified in an accession (ZB1) of P. tenella. Through yeast two-hybrid assays, it indicated that there were interactions between PetSBP1 and PetCUL1 and between PetSBP1 and PetSFBs (SFB16 and SFB17), and subsequent pull-down assays confirmed these interactions, suggesting a novel SBP1-containing SCFSFB complex in wild dwarf almond. Moreover, expect for putative interaction between PetSSK1 and PetCUL1, it revealed that PetSSK1 could not interact with PetSFB16 and PetSFB17, suggesting that the canonical SSK1-containing SCFSFB complex could not be identified. It further first suggested a novel molecular mechanism of gametophytic SI in Prunus species.

Keywords: self-incompatibility, Sbp1, SCF (SKP1–Cullin1–F-box-Rbx1) complex, Prunus, dwarf almond

Received: 13 Mar 2019; Accepted: 24 Sep 2019.

Copyright: © 2019 Zeng, Wang, Hao, Yu, Abudukayoumu, Tang, Zhang and Ma. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Dr. Bin Zeng, College of Forestry and Horticulture, Xinjiang Agricultural University, Urumqi, China, zbxjau@163.com