Original Research ARTICLE
Analyzing the effects of intrauterine hypoxia on gene expression in oocytes of rat offspring by single cell transcriptome sequencing
- 1department of pediatrics, Xiangya Hospital, Central South University, China
- 2Department of Physiology, Xiangya School of Medicine, Central South University, China
Intrauterine hypoxia is one of the most frequently occurring complications during pregnancy, and the effects of antenatal hypoxia in offspring are not restricted to the perinatal period. Previous studies have reported on this phenomenon, which is usually described as multigenerational or transgenerational inheritance. However, the exact mechanism of this type of inheritance is still not clear. Therefore, in the present study, we investigated the alteration in the gene expression of oocytes, derived from intrauterine hypoxia rats and their offspring, by single cell transcriptome sequencing. Our results showed that 11 differentially expressed genes (DEGs) were inherited from the F1 to F2 generation. Interestingly, these DEGs were enriched in processes predominantly involved in lipid and insulin metabolism. Overall, our data indicated that alteration in the gene expression of oocytes may be associated with some metabolic diseases and could potentially be the basis of transgenerational or multigenerational inheritance, induced by an adverse perinatal environment.
Keywords: Intrauterine hypoxia, oocyte, Single cell sequencing, Transcriptome sequencing analysis, Multigenerational inheritance
Received: 18 May 2019;
Accepted: 11 Oct 2019.
Copyright: © 2019 Li, Liu, Yue, Liao, Luo, Wang, Cao, Ding and Lin. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Mx. Shaojie Yue, Xiangya Hospital, Central South University, department of pediatrics, Changsha, 410008, Hunan Province, China, email@example.com
PhD. Zhengchang Liao, Xiangya Hospital, Central South University, department of pediatrics, Changsha, 410008, Hunan Province, China, firstname.lastname@example.org