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Front. Aging Neurosci. | doi: 10.3389/fnagi.2018.00034

Urinary 8-oxo-7,8-dihydroguanosine as a potential biomarker of aging

 Wei Gan1, 2,  Xin-Le Liu1, Ting Yu1,  Yuan-Gao Zou1, Ting-Ting Li1,  Shuang Wang1, Jin Deng1, Lan-Lan Wang1 and  Jian-Ping Cai2*
  • 1West China Hospital of Sichuan University, China
  • 2The MOH Key Laboratory of Geriatrics, Beijing Hospital, National Center of Gerontology, China

BACKGROUND: A molecular biomarker of physiologic age, as opposed to chronologic age, is needed in clinical medicine. 8-oxo-7,8-dihydro-2’-deoxyguanosine (8-oxodGuo) and 8-oxo-7, 8-dihydroguanosine (8-oxoGuo) are two promising aging biomarkers.
METHODS: A total of 1228 healthy Chinese residents (613 males and 615 females) 2 to 90 years of age were randomly selected. Spot urine samples were collected, and the concentrations of 8-oxodGuo and 8-oxoGuo were measured using ultra-high-performance liquid chromatography with a triple quadrupole mass spectrometer (UPLC-MS/MS). Method validation, including accuracy, precision, linearity and quantification limit, was performed. The relationship between oxidized guanosine and age/gender was evaluated.
RESULTS: 8-OxodGuo and 8-oxoGuo were eluted at 1.61 and 1.30 minutes, respectively. The calibration curve was linear in the range of 0.2–500 ng/ml for both analytes. The lowest limit of quantification (LLOQ) was 0.2 ng/ml for 8-oxodGuo and 0.1 ng/ml for 8-oxoGuo. There was an age-dependent increase in the biomarkers from the 21- to 30-year-old group to the 81- to 90-year-old group in both genders. In the subjects older than 61 years of age, the levels of 8-oxodGuo as well as 8-oxoGuo in urine were much higher in females than in males. The content of 8-oxoGuo correlated more closely with age and was higher (approximately 2-fold) than that of 8-oxodGuo for a given individual.
CONCLUSIONS: 8-oxodGuo and 8-oxoGuo can be easily measured by UPLC-MS/MS. Urinary 8-oxoGuo may be a potential biomarker to determine a person’s physiologic age and identify individuals at high risk of developing age-associated disease.

Keywords: 8-OxoGuanosine, Urine Specimen Collection, Mass Spectrometry, Method validation, biomarkers of aging

Received: 25 Sep 2017; Accepted: 30 Jan 2018.

Edited by:

Hanting Zhang, West Virginia University, United States

Reviewed by:

Rongqiao He, State Key Laboratory of Brain and Cognitive Science, Institute of Biophysics (CAS), China
Suowen Xu, University of Rochester, United States  

Copyright: © 2018 Gan, Liu, Yu, Zou, Li, Wang, Deng, Wang and Cai. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Dr. Jian-Ping Cai, The MOH Key Laboratory of Geriatrics, Beijing Hospital, National Center of Gerontology, Beijing, China, caijp61@vip.sina.com