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Front. Aging Neurosci. | doi: 10.3389/fnagi.2018.00389

Protective effect of hydrogen on sodium iodate-induced age related macular degeneration in mice

 Lihua Li1*, Yanli Liu1, Ruichan Li1, Jing xie1, Jiehua Hu2 and  Fu Ren3
  • 1Taizhou University, China
  • 2Naval University of Engineering, Logistics college, Information Center,, China
  • 3Jinzhou Medical University, China

Oxidative stress is one of the main causes of AMD. Hydrogen have anti-oxidative stress and apoptotic effects on retinal injury. However, the effect of hydrogen on AMD is not clear. In this study, fundus radiography, OCT and FFA demonstrated that HRW reduced the deposition of drusen-like structures in RPE layer, prevented retina from thinning and leakage of ocular fundus vasculature by induced by NaIO3. ERG analysis confirmed that HRW effectively reversed the decrease of a-wave and b-wave amplitude in NaIO3-mice. Mechanistically, HRW greatly reduced the oxidative stress reaction through decreased MDA levels, increased SOD production, and decreased ROS content. The OGG1 expression was down-regulated which is marker of oxidative stress. Involvement of oxidative stress was confirmed using oxidative stress inhibitor ALCAR. Moreover, oxidative stress reaction was associated with expression of Sirt1 level and HRW significantly inhibited the downregulation of Sirt1 expression. This result was further confirmed with AICAR which restore Sirt1 expression and activity. In addition, NaIO3-induced retinal damage was related to apoptosis via caspase 8 and caspase 9, but not the caspase 3 pathways, which led to upregulation of Bax and p53, downregulation of Bcl-2 and increase in Jc-1-positive cells in mice. However, HRW effectively reversed these effects that apoptosis induced. These results suggest that HRW protects retinal functions against oxidative stress injury through inhibiting downregulation of Sirt1 and reducing retinal apoptosis. Therefore, we speculated that hydrogen administration is a promising treatment for AMD therapy.

Keywords: SIRT1, Oxidative Stress, Apoptosis, AMD, Hydrogen

Received: 01 Aug 2018; Accepted: 06 Nov 2018.

Edited by:

Walter E. Müller, Goethe-Universität Frankfurt am Main, Germany

Reviewed by:

Ramesh Kandimalla, Texas Tech University Health Sciences Center, United States
Xue-Jun Sun, Second Military Medical University, China  

Copyright: © 2018 Li, Liu, Li, xie, Hu and Ren. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Prof. Lihua Li, Taizhou University, Taizhou, China,