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Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Cell. Neurosci. | doi: 10.3389/fncel.2019.00358

A kinetic map of the homomeric voltage-gated potassium channel (Kv) family

 Rajnish Ranjan1*,  Emmanuelle LOGETTE1, Michela Marani2, Mirjia Herzog1, Valerie Tache1 and  Henry Markram1
  • 1Blue Brain Project, Ecole polytechnique fédérale de Lausanne (EPFL), Switzerland
  • 2École Polytechnique Fédérale de Lausanne, Switzerland

The voltage-gated potassium (Kv) channels, encoded by 40 genes, repolarize all electrically excitable cells, including plant, cardiac and neuronal cells. Although these genes were fully sequenced decades ago, a comprehensive kinetic characterization of all Kv channels is still missing, especially near physiological temperature. Here, we present a standardized kinetic map of the 40 homomeric Kv channels systematically characterized at 15°C, 25°C and 35°C. Importantly, the Kv kinetics at 35°C differ significantly from commonly reported kinetics, usually performed at room temperature. We observed voltage-dependent Q10 for all active Kv channels and inherent heterogeneity in kinetics for some of them. Kinetic properties are consistent across different host cell lines and conserved across mouse, rat and human. All electrophysiology data from all Kv channels are made available through public website (Channelpedia). This dataset provides a solid foundation for exploring kinetics of heteromeric channels, roles of auxiliary subunits, kinetic modulation, and for building accurate Kv models.

Keywords: ion channel, Kv, automated patch clamp electrophysiology, CHO - Chinese hamster ovary, Kinetics

Received: 28 Jan 2019; Accepted: 19 Jul 2019.

Edited by:

Maria C. D'Adamo, University of Malta, Malta

Reviewed by:

Robert Brenner, The University of Texas Health Science Center at San Antonio, United States
Pietro Balbi, Department of Neurorehabilitation, IRCCS Istituti Clinici Scientifici Maugeri (ICS Maugeri), Italy  

Copyright: © 2019 Ranjan, LOGETTE, Marani, Herzog, Tache and Markram. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Dr. Rajnish Ranjan, Blue Brain Project, Ecole polytechnique fédérale de Lausanne (EPFL), Geneva, 1202, Geneva, Switzerland, ranjan.rajnish@epfl.ch