Culture Model for Non-Human Primate Choroid Plexus
- 1Microbiology & Immunology, Tulane University School of Medicine, United States
- 2Tulane National Primate Research Center, School of Medicine, Tulane University, United States
While there are murine and rat choroid plexus epithelial cell cultures, a translationally relevant model for choroid plexus activation and function in primates is still lacking. The rhesus macaque is gold standard for modeling viral infection and activation of CNS, including HIV-associated neurocognitive disorders. We have developed a rhesus macaque choroid plexus epithelial cell culture model which we believe to suitable for studies of inflammation associated with viral infection of the CNS. Epithelial morphology and function were assessed using vimentin, phalloidin, the tight junction protein zonula-occludens-1 (ZO-1), and focal adhesion kinase (FAK). Choroid plexus epithelial cell type was confirmed using immunofluorescence with two proteins highly expressed in the choroid plexus: transthyretin and -klotho. Finally, barrier properties of the model were monitored using pro- and anti-inflammatory mediators (TNF-, the TLR2 agonist PamCys3K, and dexamethasone). We have presented a model of the blood-CSF barrier that will allow study into pro- and anti-inflammatory conditions in the brain, while simultaneously measuring real time changes to epithelial cells.
Keywords: Choroid Plexus, epithelial cell, cell culrture based methods, rhesus macaque, Aging, Infectious Disease
Received: 19 Jun 2019;
Accepted: 15 Aug 2019.
Edited by:Arturo Ortega, Center for Research and Advanced Studies (CINVESTAV), Mexico
Reviewed by:Hugo Guerrero-Cazares, Mayo Clinic, United States
Esther López-Bayghen, Center for Research and Advanced Studies (CINVESTAV), Mexico
Copyright: © 2019 MacLean and Delery. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Dr. Andrew MacLean, Tulane University School of Medicine, Microbiology & Immunology, New Orleans, 70433, LA, United States, firstname.lastname@example.org