Brief Research Report ARTICLE
Observations from a mouse model of forebrain Voal knockout: focusing on hippocampal structure and function
- 1First Affiliated Hospital of Jilin University, China
- 2Krembil Research Institute, University Health Network, Canada
Voa protein is a subunit of V-ATPase proton pump which is essential to acidify intracellular organelles including synaptic vesicles. Voa1 is one of the four isoforms of Voa family with strong expression in neurons. To examine the role of Voa1 protein in mammalian brain neurons, we generated conditional Voa1 knockout mice in which Voa1 was selectively deleted from forebrain pyramidal neurons. We examined the Voa1 knockout mice of ages 5-6 months to assess persistent effects of Voa1 deletion. The Voa1 knockout mice exhibited poor performance in the Morris water maze test compared to control mice. In addition, synaptic field potentials of the hippocampal CA1 were greatly diminished in the Voa1 knockout mice when examined in brain slices in vitro. Furthermore, the Voa1 knockout mice presented severe degeneration of dorsal hippocampal CA1 neurons while CA3 neurons were largely preserved. The CA1 neurodegeneration was associated with general brain atrophy as indicated by an overall decrease in hemispheric areas. Despite the CA1 degeneration and dysfunction, the Voa1 knockout mice exhibited hippocampal CA3 electroencephalographic spikes and non-convulsive discharges. These hippocampal spikes were suppressed by single intra-peritoneal injection of diazepam which is a benzodiazepine GABAa receptor enhancer. Together these results suggest that Voa1 related activities are essential for survival of the targeted neurons in the dorsal hippocampal CA1 as well as other forebrain areas. We postulate that the Voa1 knockout mice may serve as a valuable model for further investigation of V-ATPase dysfunction related neuronal degeneration and functional abnormalities in forebrain areas particularly the hippocampus.
Keywords: V-ATPase, spatial memory, Glutamate transamination, Neuronal degeneration, synapse, brain slice, EEG, Conditional knock out mice
Received: 22 Jul 2019;
Accepted: 11 Oct 2019.
Copyright: © 2019 Ma, Bin, Shi, Harada, Monnier, Sugita and Zhang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Dr. Shan Shi, First Affiliated Hospital of Jilin University, Changchun, 130021, Jilin Province, China, firstname.lastname@example.org
Dr. Shuzo Sugita, Krembil Research Institute, University Health Network, Toronto, Ontario, Canada, Shuzo.Sugita@uhnresearch.ca