Impact Factor 5.076

The Frontiers in Neuroscience journal series is the 1st most cited in Neurosciences

Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Mol. Neurosci. | doi: 10.3389/fnmol.2018.00050

Topical Dexamethasone Administration Impairs Protein Synthesis and Neuronal Regeneration in the Olfactory Epithelium

Umberto Crisafulli1, 2,  Andre M. Xavier1,  Fabiana B. Santos1, Tavane D. Cambiaghi3,  Seo Y. Chang1, Marimelia Porcionatto1, Beatriz A. Castilho3,  Bettina Malnic2 and  Isaias Glezer1*
  • 1Department of Biochemistry, Escola Paulista de Medicina, Universidade Federal de São Paulo, Brazil
  • 2Department of Biochemistry, Instituto de Química, Universidade de São Paulo, Brazil
  • 3Department of Microbiology, Immunology and Parasitology, Escola Paulista de Medicina, Universidade Federal de São Paulo, Brazil

Chronic inflammatory process in the nasal mucosa is correlated with poor smell perception. Over-activation of immune cells in the olfactory epithelium (OE) is generally associated with loss of olfactory function, and topical steroidal anti-inflammatory drugs have been largely used for treating such condition. Whether this therapeutic strategy could directly affect the regenerative process in the OE remains unclear. In this study we show that nasal topical application of Dexamethasone (DEX; 200 or 800 ng/nostril), a potent synthetic anti-inflammatory steroid, attenuates OE lesion caused by gram-negative bacteria lipopolysaccharide (LPS) intranasal infusion. In contrast, repeated DEX (400 ng/nostril) local application after lesion establishment limited the regeneration of olfactory sensory neurons (OSNs) after injury promoted by LPS or methimazole. Remarkably, DEX effects were observed when the drug was infused as three consecutive-days regimen. The anti-inflammatory drug does not induce OE progenitor cell death, however, disturbance in mTOR downstream signaling pathway and impairment of protein synthesis were observed during the course of DEX treatment. In addition, in vitro studies conducted with OE neurospheres in the absence of an inflammatory environment showed that glucocorticoid receptor (GR) engagement directly reduces OE progenitor cells proliferation. Our results suggest that DEX can interfere with the intrinsic regenerative cellular mechanisms of the OE, raising concerns on the use of topical anti-inflammatory steroids as a risk factor for progressive olfactory function impairment.

Keywords: Anosmia, Inflammation, Corticoids, Dexamethasone, Glucocorticoid receptor, innate immune response, lipopolysaccharide (LPS), Neurogenesis, Neurosphere, Neuronal cell death, olfactory epithelium, Olfactory Marker Protein, Olfactory sensory neurons, protein synthesis, rapamycin, Regeneration, S6 kinase, Toll-Like Receptor 4, mTOR pathway, mTORC1

Received: 30 Nov 2017; Accepted: 06 Feb 2018.

Edited by:

Teresa Duda, Salus University, United States

Reviewed by:

Sulev Kõks, University of Tartu, Estonia
Sadaharu Miyazono, Asahikawa Medical University, Japan  

Copyright: © 2018 Crisafulli, Xavier, Santos, Cambiaghi, Chang, Porcionatto, Castilho, Malnic and Glezer. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Dr. Isaias Glezer, Escola Paulista de Medicina, Universidade Federal de São Paulo, Department of Biochemistry, Rua 3 de maio, 100, 5o Andar, São Paulo, 04044-020, São Paulo, Brazil, iglezer@unifesp.br