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Front. Mol. Neurosci. | doi: 10.3389/fnmol.2018.00061

Accessory and Central α-helices of Complexin Selectively Activate Ca2+ Triggering of Synaptic Exocytosis

 Yi Yu1, Su Chen1, Xiaoqiang Mo2, Jihong Gong3, Chenhong Li1 and  Xiaofei Yang1*
  • 1College of Biomedical Engineering, South-Central University for Nationalities, China
  • 2Youjiang Medical College for Nationalities, China
  • 3College of Life Science and Technology, Huazhong University of Science and Technology, China

Complexins, binding to assembling SNARE complexes, activate Ca2+ triggered exocytosis and clamp spontaneous release in the presynaptic terminal. Functions of complexin are structural dependent and mechanistically distinct. To further understand the functional/structural dependence of complexin, here we show that the accessory and central α-helices of complexin are sufficient in activation of Ca2+ triggered vesicle fusion but not in clamping spontaneous release. Targeting the two α-helices to synaptic vesicle suppresses spontaneous release, thus further emphasizing the importance of curvature membrane localization in clamping function.

Keywords: Complexin, Ca2+ triggered exocytosis, spontaneous release, SNARE protein, synaptic vesicle

Received: 05 Dec 2017; Accepted: 13 Feb 2018.

Edited by:

Jiajie Diao, University of Cincinnati, United States

Reviewed by:

Xiaochu Lou, University of Texas Southwestern Medical Center, United States
Zhitao Hu, The University of Queensland, Australia
Wei Dong, Southwest Medical University, China  

Copyright: © 2018 Yu, Chen, Mo, Gong, Li and Yang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Prof. Xiaofei Yang, South-Central University for Nationalities, College of Biomedical Engineering, Wuhan, China, sunlittlefly@hotmail.com