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Front. Mol. Neurosci. | doi: 10.3389/fnmol.2018.00364

Comparative hippocampal synaptic proteomes of rodents and primates: differences in neuroplasticity-related proteins

  • 1Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Amsterdam Neuroscience, VU University Amsterdam, Netherlands
  • 2Biomedical Primate Research Centre, Netherlands

Key to the human brain’s unique capacities are a myriad of neural cell types, specialized molecular expression signatures, and complex patterns of neuronal connectivity. Neurons in the human brain communicate via well over a quadrillion synapses. Their specific contribution might be key to the dynamic activity patterns that underlie primate-specific cognitive function. Recently, functional differences were described in transmission capabilities of human and rat synapses. To test whether unique expression signatures of synaptic proteins are at the basis of this, we performed a quantitative analysis of the hippocampal synaptic proteome of four mammalian species, two primates, human and marmoset, and two rodents, rat and mouse. Abundance differences down to 1.15-fold at an FDR-corrected p-value of 0.005 were reliably detected using SWATH mass spectrometry. The high measurement accuracy of SWATH allowed the detection of a large group of differentially expressed proteins between individual species and rodent versus primate. Differentially expressed proteins between rodent and primate were found highly enriched for plasticity-related proteins.

Keywords: synapse, Hippocampus, species, neuroplasticity, Proteomics, SWATH-MS

Received: 05 May 2018; Accepted: 13 Sep 2018.

Edited by:

Hiroyuki Okuno, Graduate School of Medical and Dental Sciences, Kagoshima University, Japan

Reviewed by:

Ayse Dosemeci, National Institutes of Health (NIH), United States
Akiya Watakabe, RIKEN Center for Brain Science (CBS), Japan  

Copyright: © 2018 Koopmans, Pandya, Franke, Philippens, Paliukhovich, Li and Smit. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Mr. Frank Koopmans, VU University Amsterdam, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Amsterdam Neuroscience, Amsterdam, Netherlands, frank.koopmans@vu.nl