ORIGINAL RESEARCH article
Front. Virol.
Sec. Modeling of Viral Replication and Pathogenesis
Effects of the cross-talk between PARP12/PARP13 and non-sense mediated RNA decay pathway on RNA stability and replication of SARS-CoV-2
Provisionally accepted
- University of Texas Medical Branch at Galveston, Galveston, United States
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Nonsense-mediated mRNA decay (NMD) pathway recognizes the mRNAs of host and cytoplasmic pathogens harboring aberrant features and targets them for degradation. Poly(ADP-ribose) polymerases (PARPs) superfamily consists of 17 members, among which macrodomain and zinc finger PARPs function as regulators of RNA metabolism and transcription. In this study, we investigated whether crosstalk between NMD and PARPs regulates SARS-CoV-2 RNA stability and viral infection. For this, transgenic mice (hACE2tg) expressing human angiotensin-converting enzyme 2, and human alveolar epithelial cells (Calu-3ACE2+, A549ACE2+), in which the expression of NMD factors and PARPs was modulated by molecular approaches were used. We found that NMD pathway targets endogenous and exogenous aberrant transcripts in human lung epithelial cells. Upon SARS-CoV-2 infection, the expression of NMD factors, up-framshift 1 and 2 (UPF1/UPF2) was decreased while PARP12 and PARP13 were significantly increased in Calu-3ACE2+ and A549ACE2+ cells and lung tissues of hACE2tg mice. Depletion of PARP12/PARP13 using target-specific (vs. scrambled) siRNAs significantly enhanced the stability of NMD targeted endogenous and exogenous aberrant transcripts and SARS-CoV-2 subgenomic S, E, M, and N mRNAs in A549ACE2+ cells, like what was noted in siUPF1/siUPF2-transfected lung epithelial cells. Conversely, overexpression of PARP12/PARP13 enhanced the NMD-dependent degradation of aberrant transcripts and SARS-CoV-2 subgenomic and genomic RNAs. Further, overexpression of PARP12/PARP13 had a dose-dependent effect in enhancing the anti-viral NMD activity and suppression of SARS-CoV-2 replication in infected cells. We conclude that 3 PARP12/PARP13 synergize with NMD pathway to regulate the viral mRNA stability and replication of SARS-CoV-2.
Keywords: SARS-CoV-2, Poly(ADP)ribose polymerase, Nonsense mediated RNA decay, antiviral response, COVID - 19
Received: 22 Aug 2025; Accepted: 27 Oct 2025.
Copyright: © 2025 Lokugamage, Choudhuri, Rayavara, Tseng, Makino and Garg. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Nisha Jain Garg, nigarg@utmb.edu
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