Protoplast Regeneration | List of Frontiers open access articles

Original Research

Published on 16 Nov 2021

Establishment of an Efficient Protoplast Regeneration and Transfection Protocol for Field Cress (Lepidium campestre)

in Genome Editing in Plants

Sjur Sandgrind
Xueyuan Li
Emelie Ivarson
Annelie Ahlman
Li-Hua Zhu

Frontiers in Genome Editing

doi 10.3389/fgeed.2021.757540

7,542 views
10 citations

Review

Published on 03 Sep 2021

Protoplast Regeneration and Its Use in New Plant Breeding Technologies

in Genome Editing in Plants

Kelsey M. Reed
Bastiaan O. R. Bargmann

Frontiers in Genome Editing

doi 10.3389/fgeed.2021.734951

54,984 views
152 citations

Original Research

Published on 21 Jan 2021

Genome Editing and Protoplast Regeneration to Study Plant–Pathogen Interactions in the Model Plant Nicotiana benthamiana

in Genome Editing in Plants

Chen-Tran Hsu
Wen-Chi Lee
Yu-Jung Cheng
Yu-Hsuan Yuan
Fu-Hui Wu
Choun-Sea Lin

Frontiers in Genome Editing

doi 10.3389/fgeed.2020.627803

14,300 views
29 citations

Mini Review

Published on 11 Aug 2021

Protoplasts: From Isolation to CRISPR/Cas Genome Editing Application

in Genome Editing in Plants

Jin-Jun Yue
Jin-Ling Yuan
Fu-Hui Wu
Yu-Hsuan Yuan
Qiao-Wei Cheng
Chen-Tran Hsu
Choun-Sea Lin

Frontiers in Genome Editing

doi 10.3389/fgeed.2021.717017

25,329 views
89 citations

Original Research

Published on 19 Sep 2025

Development of a highly efficient protoplast regeneration and transfection protocol for enhancing CRISPR genome editing of Brassica carinata

in Plant Biotechnology

Xueyuan Li
Misteru Tesfaye
Sjur Sandgrind
Rui Guan
Li-Hua Zhu

Frontiers in Plant Science

doi 10.3389/fpls.2025.1604283

4,039 views

Original Research

Published on 27 Mar 2024

Phytosulfokine alpha enhances regeneration of transformed and untransformed protoplasts of Brassica oleracea

in Plant Biotechnology

Valentin Vogrinčič
Damijana Kastelec
Jana Murovec

Frontiers in Plant Science

doi 10.3389/fpls.2024.1379618

4,460 views
7 citations

Brief Research Report

Published on 30 Jun 2025

DNA-free CRISPR genome editing in raspberry (Rubus idaeus) protoplast through RNP-mediated transfection

in Genome Editing in Plants

Ryan Creeth
Andrew Thompson
Zoltan Kevei

Frontiers in Genome Editing

doi 10.3389/fgeed.2025.1589431

5,965 views
1 citation

Correction

Published on 27 Mar 2023

Corrigendum: Establishment of an efficient protoplast regeneration and transfection protocol for field cress (Lepidium campestre)

in Genome Editing in Plants

Sjur Sandgrind
Xueyuan Li
Emelie Ivarson
Annelie Ahlman
Li-Hua Zhu

Frontiers in Genome Editing

doi 10.3389/fgeed.2023.1183791

1,517 views
1 citation

Correction

Published on 17 Feb 2026

Correction: Development of a highly efficient protoplast regeneration and transfection protocol for enhancing CRISPR genome editing of Brassica carinata

in Plant Biotechnology

Frontiers Production Office

Frontiers in Plant Science

doi 10.3389/fpls.2026.1802940

130 views

Original Research

Published on 07 Jan 2025

Knockout of BnaX.SGT.a caused significant sinapine reduction in transgene-free rapeseed mutants generated by protoplast-based CRISPR RNP editing

in Plant Biotechnology

Oliver Moss
Xueyuan Li
Eu Sheng Wang
Selvaraju Kanagarajan
Rui Guan
Emelie Ivarson
Li-Hua Zhu

Frontiers in Plant Science

doi 10.3389/fpls.2024.1526941

3,089 views
4 citations

Original Research

Published on 07 Jul 2021

Efficient Protoplast Regeneration Protocol and CRISPR/Cas9-Mediated Editing of Glucosinolate Transporter (GTR) Genes in Rapeseed (Brassica napus L.)

in Plant Biotechnology

Xueyuan Li
Sjur Sandgrind
Oliver Moss
Rui Guan
Emelie Ivarson
Eu Sheng Wang
Selvaraju Kanagarajan
Li-Hua Zhu

Frontiers in Plant Science

doi 10.3389/fpls.2021.680859

21,026 views
55 citations

Correction

Published on 22 Mar 2023

Corrigendum: Efficient protoplast regeneration protocol and CRISPR/Cas9-mediated editing of glucosinolate transporter (GTR) genes in rapeseed (Brassica napus L.)

in Plant Biotechnology

Xueyuan Li
Sjur Sandgrind
Oliver Moss
Rui Guan
Emelie Ivarson
Eu Sheng Wang
Selvaraju Kanagarajan
Li-Hua Zhu

Frontiers in Plant Science

doi 10.3389/fpls.2023.1183684

2,101 views

Articles

Establishment of an Efficient Protoplast Regeneration and Transfection Protocol for Field Cress (Lepidium campestre)

Protoplast Regeneration and Its Use in New Plant Breeding Technologies

Genome Editing and Protoplast Regeneration to Study Plant–Pathogen Interactions in the Model Plant Nicotiana benthamiana

Protoplasts: From Isolation to CRISPR/Cas Genome Editing Application

Development of a highly efficient protoplast regeneration and transfection protocol for enhancing CRISPR genome editing of Brassica carinata

Phytosulfokine alpha enhances regeneration of transformed and untransformed protoplasts of Brassica oleracea

DNA-free CRISPR genome editing in raspberry (Rubus idaeus) protoplast through RNP-mediated transfection

Corrigendum: Establishment of an efficient protoplast regeneration and transfection protocol for field cress (Lepidium campestre)

Correction: Development of a highly efficient protoplast regeneration and transfection protocol for enhancing CRISPR genome editing of Brassica carinata

Knockout of BnaX.SGT.a caused significant sinapine reduction in transgene-free rapeseed mutants generated by protoplast-based CRISPR RNP editing

Efficient Protoplast Regeneration Protocol and CRISPR/Cas9-Mediated Editing of Glucosinolate Transporter (GTR) Genes in Rapeseed (Brassica napus L.)

Corrigendum: Efficient protoplast regeneration protocol and CRISPR/Cas9-mediated editing of glucosinolate transporter (GTR) genes in rapeseed (Brassica napus L.)