With over 113,000 citations across more than 9,100 articles, Frontiers in Bioengineering and Biotechnology is the most cited journal in its field. Explore some of the journal's trending articles below.

Development of a Chemically Defined Medium for in vitro Expansion of Primary Bovine Satellite Cells
The aim of this study is to develop a chemically defined, cost efficient serum-free and animal-free medium that supports the attachment and expansion of bovine myoblasts while maintaining their differentiation capacity.

The Potential of Brewer’s Spent Grain in the Circular Bioeconomy: State of the Art and Future Perspectives
Insight into the availability of brewer’s spent grain, chemical properties, and current and potential applications juxtaposed with the existing and emerging markets of the pyramid of bio-based products in the context of sustainable and circular bioeconomy

Recent developments in isolating methods for exosomes
This review summarizes a series of methods for the isolation of exosomes, with emphasis on the emerging methods, and in-depth comparison and analysis of each method are provided.

Electrospun self-emulsifying core-shell nanofibers for effective delivery of paclitaxel
In this study, a combination strategy of top-down process (electrospinning) and bottom-up (self-emulsifying) was demonstrated to be useful for enhancing the dissolution of a typical poorly water-soluble anticancer model drug.

Strategies and Considerations for Improving Recombinant Antibody Production and Quality in Chinese Hamster Ovary Cells
This review provides a comprehensive overview of several state-of-the-art approaches, such as optimization of gene sequence of antibody, construction and optimization of high-efficiency expression vector and using antibody expression system.

Phase Behaviour, Functionality, and Physicochemical Characteristics of Glycolipid Surfactants of Microbial Origin
The understanding of the physicochemical properties of biosurfactants is essential for the successful application in many branches of industry. This study focuses on Glycolipids, which are currently gaining a lot of interest for such applications.

Hello, world! VIVA+: A human body model lineup to evaluate sex-differences in crash protection
This paper is the first in a series describing the development and validation of the new Human Body Model lineup, VIVA+, to evaluate sex-differences in crash protection.

A multi-organ-on-chip to recapitulate the infiltration and the cytotoxic activity of circulating NK cells in 3D matrix-based tumor model
This paper proposes an immune-tumor organ-on-chip-based model that represents a promising approach for faithfully recapitulating the human pathology and efficiently employing the immunotherapies testing.

Methods to improve the accuracy of next-generation sequencing
Study summarizing the general procedures of next-generation sequencing platforms, highlighting the improvements involved in eliminating errors at each step.

Off-target effects in CRISPR/Cas9 gene editing
This review summarizes the technological advancements of CRISPR/Cas9 and discusses the current challenges in the management of off-target effects for future gene therapy.
Read other impactful articles published in Frontiers in Bioengineering and Biotechnology.