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Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Genet. | doi: 10.3389/fgene.2019.01223

Chloroplast Genome Sequencing and Comparative Analysis for Fine Fescue (Festuca L., Poaceae) Turfgrasses

 Yinjie Qiu1*, Cory D. Hirsch1, Ya Yang1 and Eric T. Watkins1
  • 1University of Minnesota Twin Cities, United States

Fine fescues (Festuca L., Poaceae) are turfgrass species that perform well in low-input environments. Based on morphological characteristics, the most commonly-utilized fine fescues are divided into five taxa: three are subspecies within F. rubra L. and the remaining two are treated as species within the F. ovina L. complex. Morphologically, these five taxa are very similar, and both identification and classification of fine fescues remain challenging. In an effort to develop classification methods for fescues, we used flow cytometry to estimate genome size and sequenced the chloroplast genome of all five taxa. Fine fescue chloroplast genome sizes ranged from 133,331 to 133,841 bp and contained 113-114 genes. Phylogenetic reconstruction using whole plastid genome sequences agreed with previous work based on morphology. Comparative genomics suggested unique repeat signatures for each fine fescue taxon and could potentially be used for marker development for taxon identification.

Keywords: Fine fescues, chloroplast, comparative genome analysis, Turfgrass, Turfgrass breeding

Received: 15 Aug 2019; Accepted: 05 Nov 2019.

Copyright: © 2019 Qiu, Hirsch, Yang and Watkins. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Mr. Yinjie Qiu, University of Minnesota Twin Cities, St. Paul, 55108, Minnesota, United States, qiuxx221@umn.edu