Original Research ARTICLE
Sliding perspectives: dissociating ownership from self-location during full body illusions in virtual reality
- 1EVENT Lab, Facultat de Psicologia, Universitat de Barcelona, Barcelona, Spain
- 2Institució Catalana Recerca i Estudis Avancats, Barcelona, Spain
Bodily illusions have been used to study bodily self-consciousness and disentangle its various components, among other the sense of ownership and self-location. Congruent multimodal correlations between the real body and a fake humanoid body can in fact trigger the illusion that the fake body is one's own and/or disrupt the unity between the perceived self-location and the position of the physical body. However, the extent to which changes in self-location entail changes in ownership is still matter of debate. Here we address this problem with the support of immersive virtual reality. Congruent visuotactile stimulation was delivered on healthy participants to trigger full body illusions from different visual perspectives, each resulting in a different degree of overlap between real and virtual body. Changes in ownership and self-location were measured with novel self-posture assessment tasks and with an adapted version of the cross-modal congruency task. We found that, despite their strong coupling, self-location and ownership can be selectively altered: self-location was affected when having a third person perspective over the virtual body, while ownership toward the virtual body was experienced only in the conditions with total or partial overlap. Thus, when the virtual body is seen in the far extra-personal space, changes in self-location were not coupled with changes in ownership. If a partial spatial overlap is present, ownership was instead typically experienced with a boosted change in the perceived self-location. We discussed results in the context of the current knowledge of the multisensory integration mechanisms contributing to self-body perception. We argue that changes in the perceived self-location are associated to the dynamical representation of peripersonal space encoded by visuotactile neurons. On the other hand, our results speak in favor of visuo-proprioceptive neuronal populations being a driving trigger in full body ownership illusions.
A large body of experimental research in cognitive neuroscience has exploited bodily illusions to study bodily self-consciousness. This work has established a tight link between self-consciousness and the processing of multisensory bodily signals performed in specific brain areas (Makin et al., 2008; Ehrsson, 2011; Blanke, 2012). It further allowed disentangling distinct components of bodily self-consciousness and their candidate neural representations (Longo et al., 2008; Blanke and Metzinger, 2009; Tsakiris et al., 2010).
The present study focuses on the mutual relation between two important components of bodily self-consciousness: the feeling of owning a body (body ownership) and the experience of the body occupying a given portion of space in the environment (self-location). Although in normal conditions ownership and self-location are tightly tied, recent experimental work has shown that these two components are, at least partially, separable (Longo et al., 2008). However, so far it has not been explored whether these two components are intrinsically coupled or are rather dissociable despite their strong coupling (Serino et al., 2013). In this article we present a study performed with the support of virtual reality (VR) technology, and designed to specifically test the hypothesis that, under specific experimental conditions, ownership and self-location can be selectively altered. The rationale behind the current study is based on a series of previous studies that are reviewed in the following.
Experimental Manipulations of the Sense of Self-Location
Self-location has been mainly investigated through experimentally induced out-of-body experiences (OBE). The experimental paradigm to induce an OBE in healthy subject consists in participants wearing a head-mounted display (HMD) occluding the physical body and displaying the back of their own body -or that of a mannequin- as filmed from a distance of roughly two meters. The visual perspective then coincides with the location of the real body and is dissociated from the location of the virtual body seen through the HMD. The illusion is then triggered through congruent visuotactile stimulation on the real and fake body. When synchronous visuotactile stimulation is delivered on the back (Lenggenhager et al., 2007), most participants experience the illusion of looking at their body from the outside, similarly to what is reported in OBEs of neurological origin (Blanke and Mohr, 2005). This illusion is associated with illusory changes in self-location, with a systematic shift from the participant's visual perspective toward the seen body. Such a drift has been consistently found throughout a variety of assessments: the “walking task ” (Lenggenhager et al., 2007) and the “mental ball dropping” task (Lenggenhager et al., 2009) provided explicit measures of the perceived self-location in external and body-centered reference frames respectively, while the crossmodal congruency task (CCT) was used to implicitly assess self-location through the localization of tactile events in the external space (Aspell et al., 2009).
Functional magnetic resonance imaging (fMRI) revealed that these illusory changes in self-location correlate with activity in the temporo-parietal junction (TPJ) and the extrastriate body area (EBA) (Ionta et al., 2011). The implication of the TPJ was consistently found in lesion studies on neurological patients suffering aberrations in self-location (Blanke et al., 2004). Focal electrical stimulation of the TPJ was further shown to systematically trigger OBE (Blanke et al., 2002).
In a similar experimental setting, OBE illusions have been alternatively triggered by delivering synchronous visuotactile stimulation on the chest while displaying the visual stimuli at the location of the occluded physical body (Ehrsson, 2007). In contrast to what found in the “back stroking” case, participants tended to feel located at the position of the physical body; this was implicitly assessed by asking participants to rate how much they felt at the location were they saw their body or at the camera's (i.e., the physical body's) location (Guterstam and Ehrsson, 2012). The difference is probably due to the fact that, in the “chest stroking” case there is no spatial conflict between visual and tactile cues. In the “back stroking” case, it probably the spatial misalignment of visual and tactile synchronous cues that induces a recalibration in the perceived self-location.
Experimental Manipulations of the Sense of Ownership
The sense of ownership toward a full body has been largely studied making use of the full body ownership illusion (FBOI), defined as the continuous and consciously impermeable feeling that a virtual/fake full body, including all its body parts, belongs to us in its integrity.
Built up on the rich experimental literature on the rubber hand illusion (Botvinick and Cohen, 1998), the experimental paradigm to elicit a FBOI consists in outfitting participants with an immersive HMD that occludes the real body and displays in its place a fake humanoid body, which may be a filmed mannequin (Petkova and Ehrsson, 2008) or a virtual character (Slater et al., 2010). Unlike the case of OBE, participants have thus a first person perspective (1PP) of the fake body usually displayed in the same posture as the real body. This setup provides participants with congruent visuo-proprioceptive cues, i.e., visual information about the virtual body's spatial configuration are congruent with perceptual cues about the body posture delivered by muscle spindle and join receptors. In the case of a static filmed mannequin, synchronous visuotactile stimulation was found to be the necessary trigger for eliciting the illusion (Petkova and Ehrsson, 2008). However, it was shown that other modalities of multisensory/multimodal correlations could trigger the illusion with no need for synchronous visuotactile stimulation. In particular, visuomotor correlations are extremely efficient in eliciting a FBOI (Slater et al., 2010; Kokkinara and Slater, 2014), thanks to the rich information processing involved in the sensorimotor control loop. On the other hand, if the fake body has a highly realistic humanoid appearance, the illusion may be elicited by congruent visuo-proprioceptive cues alone, with no need for further multimodal correlations (Maselli and Slater, 2013).
Healthy subjects experiencing a FBOI report that the seen fake body was perceived as if it was their own body and as if perceptual experiences, e.g., tactile events, were generated from the fake body. Apart from self-reports and questionnaires, the FBOI is indeed evaluated by monitoring autonomic responses to events threatening the fake body. For example, when attacking or threatening the integrity of the fake body during a FBOI, significant increases in skin conductance response (Petkova and Ehrsson, 2008) and in heart rate deceleration (Slater et al., 2010; Maselli and Slater, 2013) have been found. Importantly, the measured change in autonomic responses is positively correlated with the intensity of the illusion inferred from questionnaire scores. By monitoring brain activity with fMRI during a FBOI, it was found that the illusion correlates with activity in bilateral ventral premotor cortex (vPMc), left intraparietal cortex and left putamen (Petkova et al., 2011a). Interestingly, the ventral premotor cortex activation was found to be particularly associated with the construction of the unitary experience of a full body, ensuing from the merging of the sense of ownership of different body parts. No implication of the TPJ during a FBOI has been reported.
An alternative approach to explore the sense of ownership, complementary to the FBOI, is the “chest stroking” OBE illusion described in the previous section (Ehrsson, 2007; Guterstam and Ehrsson, 2012). Autonomic responses to threat events have shown how, in this illusion, the sense of ownership is “attached” to the not visible real body located at the position of the visual perspective (Ehrsson, 2007; Guterstam and Ehrsson, 2012), while the sense of ownership toward the body seen in the extra-personal space is disrupted (Guterstam and Ehrsson, 2012).
Do Ownership and Self-Location Have Different Neural Representations? Hints from Full Body Illusions
When comparing results from the OBE and FBOI experiments discussed above, several hints emerge suggesting that ownership and self-location might have different origins and neural representations (Maselli and Slater, 2013; Serino et al., 2013).
First, as discussed above, fMRI studies revealed that OBE and FBOI are associated with the activation of different brain areas (Ionta et al., 2011; Petkova et al., 2011a). Second, the experimental paradigms used for inducing OBE and FBOI were developed to explicitly assess either changes in self-location or in ownership, and not both at the same time.
In 1PP FBOI, self-location is not altered at all as the real and virtual body are collocated. On the other hand, the OBE experiments reviewed above have been mainly designed to accurately measure changes in the perceived self-location. The concurrent feeling of ownership toward the body seen in the extra-personal space was mainly assessed through questionnaire and free reports. When pooled together, data from questionnaires and self-reports in “back stroking” OBE show that illusory changes in self-location are not systematically associated with the sense of ownership: self-reports from some participants (Ionta et al., 2011) suggest that this illusion is associated with self-recognition rather than with the experience of owning the virtual body (e.g., “I had the impression of being touched by the stick as if I was between two mirrors and I could see my back”; “I had the impression of watching a photo of myself…”). So it is possible that questionnaire data also reveal an enhanced sense of self-recognition rather than ownership toward the virtual body (Petkova et al., 2011b; Maselli and Slater, 2013). This possibility is further supported by the fact that participants score higher to the question “It felt as if the virtual body/mannequin was my body,” when they see their own body rather than a mannequin filmed from the back (Lenggenhager et al., 2007). This modulation with the appearance of the virtual body is not found instead in 1PP illusion, in which the virtual body could importantly differ from the real in terms of age, gender and race, without reducing the illusion (e.g., Slater et al., 2010; Peck et al., 2013).
Only with few studies attempted so far to assess ownership in the “back stroking” OBE paradigm through autonomic measures: widespread decrements in body temperature associated with this OBE illusion have been recently reported (Salomon et al., 2013) and, based on previous RHI analogous results (Moseley et al., 2008), interpreted as a shift of the sense of ownership from the real to the virtual body. However, these results are not conclusive, as the effects found were smaller than the thermometer's resolution. Pomés and Slater (2013) measures instead heart rate deceleration (HRD) and movements in responses to a threat to the virtual body. Although no significant modulation of the HRD was found, their multivariate analysis suggests that the sense of ownership can positively contribute to sensations of drifting toward the virtual body. Still, these results do not provide any direct evidence for modulations of ownership being systematically driven by an altered sense of self-location.
Upon direct comparison between 3PP and 1PP conditions, it has been systematically found that ownership is significantly suppressed in 3PP with respect to 1PP (Slater et al., 2010; Petkova et al., 2011b; Maselli and Slater, 2013). Results from the “chest stroking” OBE illusions further support these finding, showing how, in this full body illusion the sense of ownership toward the real body seen from a 3PP is disrupted (Guterstam and Ehrsson, 2012).
In summary, no direct experimental evidence is currently available for effective changes in self-location driven by OBE illusions to imply systematic changes in ownership. This scenario yields the motivation for the present study. In fact, the current experimental evidence suggests that ownership and self-location may have different neural representations and that they may be -at least to some extent- dissociated from each other. In this study we explicitly tested this hypothesis and further addressed the issue of how ownership and self-location interact when both are simultaneously altered in controlled experimental setups. The study is based on a set of three independent experiments conducted with the support of immersive virtual reality.
The Cross-Modal Congruency Task as a Measure of Self-Location
An important part of the results presented in this work relies on the use of the cross-modal congruency task (CCT). In this section we give a short introduction to clarify the principles that validate the CCT, under specific configurations, as a tool to measure self-location in external space.
The CCT is a standard psychophysics test that has been designed to study interactions between vision and touch (Spence et al., 2008). The task consists of discriminating the elevation of perceived target vibrations in the presence of visual distracter stimuli (Driver and Spence, 1998b). In the classical configuration an array of four vibrators and four light emitting diodes are arranged on the participant's hands (two on the indexes and two on the thumbs). On each trial a vibration and a light flash are presented and the participant has to make a rapid judgment on whether the vibration was up (on the index) or down (on the thumbs), irrespectively of the side and of the location where the flashing light switched on. A large number of studies have consistently shown that vibrotactile discrimination is delayed and less accurate when the distracting visual cue is incongruent in elevation with the target vibration. The effect is quantified in terms of the cross-modal congruency effect (CCE), defined as the difference in performances (e.g., response times or error rates) between incongruent and congruent trials. Because visuotactile interactions are stronger when the visual cue is presented closer to the target tactile stimulation, i.e., on the same side/hand, CCE values are typically larger when computed for trials with visual and tactile cues on the same side with respect to the opposite side (Spence et al., 2004).
Three different processes have been proposed as responsible for the observed visuotactile interaction: cuing of exogenous spatial attention, multisensory integration (MSI), and response competition (light distracters priming the response) (Spence et al., 2004). These different mechanisms may dominate the CCE or contribute to it in combination with others, depending on the time separation, i.e., the stimulus onset asynchrony (SOA), between the target and the distracter stimulus. MSI mechanisms dominate the CCE for SOA smaller than 100 ms (irrespectively of the stimuli order), while for SOAs outside this range the effect is mainly driven by exogenous attention or priming effects (Shore et al., 2006).
Using an appropriate SOA, the CCT can be therefore used as a tool for detecting the MSI of visual and tactile stimuli. The crossmodal effect found in a variety of spatial configurations can be indeed explained on the basis of the known spatial properties of bimodal visuotactile (VT) neurons found in the human and non-human primate's brain: the effect is preserved when the hands are crossed (Driver and Spence, 1998a; Maravita et al., 2003), showing how visual distracters are processed in the corresponding hand-centered reference frame, rather than in retinotopic coordinates. An analogous modulation is found in VT neural populations having visual receptive fields (vRFs) that shift in space along with body movements (Graziano and Gross, 1993; Duhamel et al., 1998; Lloyd et al., 2003; Brozzoli et al., 2012). Interestingly, crossmodal interactions were found also when the visual distracters were located on tools that have been actively manipulated (Maravita et al., 2002). This provided an additional behavioral counterpart for the properties of parietal VT neurons in the macaque monkey whose vRFs extend outwards after active tool manipulation (Iriki et al., 1996).
All these results are crucial in pointing out how the CCT can be exploited to infer the location of tactile events as perceived in external space. Following this rationale, previous studies used the CCT to test the spatial recalibration of the limb position occurring during a rubber hand illusion (RHI): effective crossmodal interactions were found despite the fact that vibrators and visual distracters (located on the rubber hands) were not aligned in external space (Pavani et al., 2000; Zopf et al., 2011), but only when the illusion was experienced. These results showed a recalibration of the perceived location the tactile stimuli, hence of real hands, toward the location of the rubber hands.
Similarly, the CCT has been adopted to infer shifts in the perceived location of the whole body, i.e., to test self-location, during OBEs (Aspell et al., 2009), with the arrays of lights and vibrators located on the back of the virtual and physical bodies respectively. An effective crossmodal interaction was found during the experienced OBE illusion, but only adopting a SOA of 233 ms, which falls in the regime in which crossmodal congruency effects are dominated by exogenous spatial attention rather than by MSI (Shore et al., 2006). No effect was found however for SOA range dominated by MSI effects, thus the interpretation of Aspell et al.'s results as due to illusory change in self-location is not straightforward.
Materials and Methods
A total of 51 subjects were recruited for the study. Experiment 1 involved 15 naïve participants (12 females) with average age 21.6 years (±3.7 years SD); Experiment 2, 17 naïve participants (8) with average age 20.4 years (±2.3 years SD); Experiment 3, 19 naïve participants (10 females) with average age 22.7 years (±5.0 years SD). Participants in all the three groups had little or no previous experience in virtual reality (scores to the question “Have you ever experienced virtual reality before?” on a Likert scale from 1 to 7 had a median of 1 and interquartile range (IQR) of 1 for all the three experiments). All participants had normal or corrected to normal vision and had no history of neurological or psychiatric disorders. Participants signed an informed consent form before taking part to the experiment, and received a compensation of €10 after completion. The experimental protocol was approved by the “Comité Ético de Investigación” of the University of Barcelona, in line with the institutional ethics and national standards for the protection of human participants. In accordance with ethical commitments, all participants were contacted by email within 3–6 weeks after the experiment, and were asked whether they had any positive or negative thoughts about their experience in the experiment. None of the participants reported negative post-experimental sensations.
Experiments were implemented and conducted with the support of VR technology. The virtual environment was a faithful reproduction of the laboratory in which the experiments took place, modeled in 3D Studio Max by a graphics artist (Figure 1). The environment was controlled with the Unity1 platform. Participants entered the virtual environment through a stereo NVIS nVisor SX1112 HMD (dual SXGA displays with 76°H × 64°V field of view (FOV) per eye, with 50°(66%) of overlap in the horizontal axis, resulting in a total field of view of 102° horizontal and 64° vertical; image resolution: 1280 × 1024 per eye; frame rate: 60 Hz). Head tracking was performed by a six degree of freedom (DoF) Intersense IS-900 device based on a coupling of infrared monitoring and inertial technology3. The tracked data were streamed in real time using the Virtual Reality Peripheral Network4 (VRPN) protocol to the Unity project.
Figure 1. Experimental setup. The virtual environment (B) was a replication of the laboratory in which the experiment took place (A). Participants experienced the environment through a stereo wide field-of-view head mounted display. Four vibrators, controlled by an Arduino board, were fixed on the participant legs for the cross-modal congruency task. A cardboard tube was used to deliver continuous visuotactile stimulations symmetrically on the two legs in some of the experimental conditions.
Visuotactile stimulation was delivered on the participant legs with a tracked cardboard tube, 70 cm in length and 5 cm in diameter. The tube's movements were tracked using the Tracking Tools toolkit of Optitrack5, a system form object motion tracking that uses 12 infrared cameras. Data from a 3D optical marker attached to the physical tube were streamed in real time (via the VRPN) to the Unity project and were used to control the movements of a virtual tube. Two optical markers were used to register the exact position of the knees of the participants with those of the virtual avatar, and other two were used in Experiment 3 for monitoring the position of the two hands. The length of the avatar's legs was scaled so to match the actual legs length for each participant. This allowed a precise colocation of the physical and virtual bodies in the 1PP condition, an exact spatial overlap of the vibrators arranged on the real legs and the virtual light distracters (LD), and further an accurate displacement of the virtual tube, which assured it to be displayed at exactly the same location on the real and virtual legs.
A set of four small vibrating motors6 was used for the CCT. The motors (3 V, 100 mA, 50 Ω, 1200 ± 300 r.p.m.) were controlled through an Arduino board connected to the Unity project. Participants used an Intersense joystick to give responses in the CCT and in the “Tube Task” described below.
Response Variables: Measures, Data Processing and Statistical Methods
Crossmodal Congruency Test (CCT)
The CCT was used in our experiments to test possible shifts in the perceived self-location toward the virtual body. The rational for this choice has been exposed in details in Section The Cross-Modal Congruency Task as a Measure of Self-location. The array of LDs/vibrators used for the CCT was arranged on the virtual/physical legs. This novel configuration had been set to detect effects of multisensory integration of tactile events on the real body and visual events on the virtual body, while having different visual perspectives of the virtual body. The four vibrators were positioned on the participant's legs using elastic bands: two on the thighs, 10 cm above the knees, and two on the external side of the shins (on the tibialis anterior muscle), 10 cm below the keens. Four virtual LDs (round red lights with ~120 mm2 surface) were located in the corresponding positions on the virtual body. Each trial of the CCT consisted of a visual cue switching on during 33 ms and followed, after a 50 ms SOA, by a target vibration of 100 ms. Due to different delays in the video stream to the HMD and in the vibrators' activation (measured in the laboratory with an oscilloscope device), the effective SOA was about 20 ms with the light stimuli leading vibrations. Participants were instructed to keep their gaze on a fixation point (a blue dot in the middle of the LDs array) and, for each trial, to indicate as fast and as more accurately as possible whether the delivered target vibration was above (i.e., on the thighs) or below (i.e., on the lower legs), irrespectively of the side and of the LD's location. Participants gave responses using the upper (for above) and lower (for below) buttons of a joystick held in their right hand.
Each CCT run consisted of 180 trials: 160 experimental trials, 40 for each of the four possible combinations of LD and vibration locations, plus 20 “no-go” trials. In the “no-go” trials the fixation point changed color (from blue to fuchsia) and participants were instructed to stop giving responses even if target vibrations were released. “No-go” trials were included in order to make sure that participants kept their eyes open and focused on the fixation point throughout the whole duration of the task (Zopf et al., 2010); subjects that responded to more than 30% of the no-go trials were excluded from the CCT data analysis. The 180 trials were presented in a random order.
The CCT data were initially processed to extract the average response time (RT) and error percentage (EP) for each participant as a function of side (left leg vs. right leg), congruency (in elevation) and experimental condition. Trials with incorrect responses or with RT larger than 1500 ms or smaller than 200 ms, were discarded. The two measures were then combined into the inverse efficiency (IE) score, defined as the ratio between the reaction time and the percentage of correct responses [IE = RT/(1 − EP)], which provides the most informative summary of the data available and compensate for possible speed-accuracy trade-offs (Townsend and Ashby, 1983; Kennett et al., 2001; Spence et al., 2001).
We chose to perform the statistical analysis on the IE data because of the better informative content of this variable with respect to the more commonly used RT (Bruyer and Brysbaert, 2011).
The IE data were first analyzed in a Three-Way repeated measures ANOVA (3 × 2 × 2) with interactions, with factors condition, side and congruency. This can detect the cross-modal interactions in terms of a main effect of congruency -because of the longer response times and the higher error rate in incongruent trials- and any interaction between congruency and side -which shows that the cross-modal interaction is stronger for stimuli close in space-. A significant interaction among the three factors would detect critical differences among the various experimental. Pairwise comparisons were then performed running a One-Way repeated measurement ANOVA on CCE-IE (the CCE calculated on the performance variable IE) with the single factor side: as discussed above, a significant modulation of the CCE by side gives hints for a perceived spatial alignment between the arrays of vibrators and LDs, and in turns provides a proxy for the perceived self-location.
The “Tube Task” (TT)
The “Tube Task” (TT) is a novel test designed to gather measures of ownership and self-location. Participants were instructed to use a joystick to adjust the size (Experiment 1) the size plus the later position (Experiment 2) of a virtual tube, so to match with its extremities the perceive locations of their external ankles.
During the task no virtual body was displayed and a blue virtual tube was the unique visible object in the environment. The tube was displayed horizontally so to pass through the participant's ankles locations. At the beginning of the task the tube was centered on the middle point of the participant's ankles, had length of 114 cm and a fixed 5 cm diameter. Participants performed the task immediately after entering the virtual environment through the HMD. This measure provided the subject's baseline. The task was then repeated after each experimental condition.
According to the variant used in the experiment, the task returned one or two measures: ΔTubeSize, defined as the difference between the size of the tube set after each condition and its baseline value, provided a measure of changes in the perceived posture (i.e., the legs separation relative to the midline). ΔTubeShift, defines as the difference between the position of the tube set after each condition and its baseline value, provided instead an estimate for the perceived drift of the body midline, thus for self-location.
In the specific experimental design adopted in this study, we propose to assess ownership by looking at the effects of the strong visuo-proprioceptive coupling between the real and virtual body that is established during a FBOI (Maselli and Slater, 2013). The TT was then designed as tool for detecting such visuo-proprioceptive coupling: the experimental manipulations adopted included a change in posture of the virtual body whose legs spread apart while participants stayed still in the initial posture. Significantly positive values of ΔTubeSize will then be a proxy for an effective visuo-proprioceptive coupling between the real and virtual bodies, able to affect proprioception just via visual cues, and thus a proxy for the ownership illusion. Before applying this new method for testing the experimental hypotheses under study, the validity of the TT was tested in Experiment 1.
In Experiment 1, in which participants were asked to adjust only the tube's size, TT data were analyzed using One-Way repeated measures ANOVA with the single factor condition applied on the ΔTubeSize. In Experiment 2 the same analysis was applied separately to ΔTubeSize and ΔTubeShift. Pairwise comparisons between experimental conditions were then performed running paired t-tests.
The “Ankles Pointing Task” (APT)
This task was designed -through pilot studies- as an alternative to the TT for ownership assessments. As discussed in Section Discussion of the Results, we found that, while the TT is a sensitive test for ownership (via posture assessments) when only the length of the tube is controlled, it loses such sensitivity when combining ownership and self-location assessments and giving participants the possibility to change the tube's size and concurrently its lateral position. By comparing results from Experiments 1 and 2, it was indeed clear that tube's shifting option deteriorated the sensitivity in the feet separation estimate. For this reason we introduced the APT and designed it so that it could provide veridical estimates of the legs separation in a body centered reference frame, irrespectively of the visual perspective that participants were given in the different experimental conditions.
In this case participants were asked to point with the two hands the perceived location of the external ankles. Participants performed the task in complete darkness. They were instructed to first extend both arms out and then to progressively move them in, with the palms facing each other, until each hand would point the outer ankle on the correspondent side (see Movie SOM). The corresponding distance between the two hands was recorded through the tracking system (see Section Materials). As for the TT, the measure of interest was the changes in the hands' separation set after each condition with respect to its baseline value, ΔHS.
As ΔHS data from the APT Experiment 3 were not normally distributed, a Wilcoxon matched pairs test was adopted to compare the ΔHS distributions in the two experimental conditions.
A 12-items questionnaire (reported in Table 1) was used in Experiments 2 and 3 to assess the subjective level and quality of the illusory experience. Ownership toward the virtual body was assessed explicitly (my-body) and implicitly through responses related to the legs spreading event (legs-x) as well as via the clothing statement that has been previously shown to positively correlate with the explicit subjective report of ownership illusion (Slater et al., 2010; Maselli and Slater, 2013). A further item was included to contrast the sense of ownership toward the virtual body with the sense of looking at another person (s-else). Changes in self-location were assessed with explicit items related to drifting sensations. Two items were included to validate the delivery of synchronous visuotactile stimulation. Each questionnaire item was scored at the end of each experimental block on a Likert scale from 1 (not at all) to 7 (completely agree) to express the level of agreement.
For each item, scores given for different experimental conditions were compared pairwise via the non-parametric Wilcoxon matched pairs test.
Experiments: Designs and Procedures
For the three experiments a within-subjects design was adopted. Each experimental condition was presented within one experimental block. Participants went through the different conditions in a counterbalanced order across participants, so that each condition was presented in the first experimental block the same number of times.
Participants wore a pair of comfortable trousers prepared so to easily arrange the four CCT vibrators held in direct contact with the skin by elastic bands. They sit on an armchair with their foot resting on a footstool and their hands on armrests, holding a joystick with their right hand (Figure 1). The separation between the feet was adjusted by the experiments at 18 cm. Participants were instructed to not make any movement, apart from head movements, for the whole duration of each experimental block. Before starting, the experimenter provided full instructions on how to perform the CCT and the TT or APT. All experiments started with an initial training session, preceding the experimental blocks: after a brief (about 10 s) exposure to the virtual environment, with a gender-matched virtual avatar displayed from a 1PP, participants were asked to perform the TT or APT; this initial measure was stored and used as the participant's baseline. Immediately afterwards, the virtual body appeared again and a CCT training session of 30 trials was initiated.
Three experimental conditions were included: in the noBody condition the virtual body was not displayed and participants saw an empty armchair when looking down. In the Body condition the virtual body was displayed to be spatially coincident with the physical body, i.e., from a first person perspective with total overlap between the real and virtual bodies (as in the upper panel of Figure 2). The Body + VT condition was the same as the Body one, but with the addition of continuous synchronous visuotactile stimulation via the tracked tube. In the noBody condition the visual perspective was the same as the first person perspective given in the two Body conditions, just the virtual body was not visible.
Figure 2. Different visual perspectives. According to the experimental conditions, participants had three different visual perspectives on the virtual body: a first person perspective with total overlap of the real and virtual bodies (1PP-TO); a third person perspective with partial overlap between the two bodies (3PP-PO); a third person perspective with no overlap between the two bodies (3PP-NO). In the Body and Body+VT conditions of Experiment 1, the visual perspective was the same as in 1PP-TO.
After completing the training phase, participants went through the three experimental blocks, each having an average duration of 10 min. In the initial phase (~1 min) participants were asked to first explore the virtual scene moving the head around and then to focus on the virtual body (Body conditions) or on the chair (noBody). Only in the Body + VT, synchronous VT stimulation was delivered: the experimenter slowly moved the tracked tube on both legs, simultaneously and symmetrically, from the feet to the thighs (see Movie in the SOM). The CCT was then started (~8 min). In the Body + VT condition, during the CCT the tube's stroking continued symmetrical and simultaneous on both legs but was restricted to the lower part of the calf and the feet (i.e., outside the region delimited by the CCT array). At CCT completion participants were again instructed to observe the scene and focus their attention on the virtual body or the chair (~1 min), as in the initial phase. In the two Body conditions, the avatar's legs moved apart after about 1 min, with the virtual feet reaching a distance of 55 cm in 3 s; in the noBody condition participants just looked at the empty chair for the same period of time. The “Tube Task” was then performed and terminated the experimental block: as the visual perspective was not manipulated, participants were instructed and only had the possibility to control the length of the tube, not its position. The HMD was removed and participants rested from 5 to 10 min before starting the next block.
The experiment was designed to test the response variables to be adopted in Experiments 2 and 3. The three main objectives were: (1) to show that the novel version of the CCT with vibrators and visual distracters arranged on the legs is equivalent to classical version (on the hands) and thus a valid tool for self-location assessments (see Section The Cross-Modal Congruency Task as a Measure of Self-Location); (2) to show that this novel version of CCT is still sensitive for detecting visuotactile integration processes on the self-body even when irrelevant visuotactile stimulation is concurrently delivered; (3) to validate the TT as a novel objective proxy for a full body ownership illusion.
In order to validate the first two points CCT's performances should reveal strong visuotactile interactions— i.e., a CCE significantly larger on the same side, with respect to opposite side— in both Body conditions. Note that we needed to validate point (2) in order to apply the CCT in the 3PP perspectives conditions of Experiments 2 and 3: in these conditions, in fact, it is necessary to keep providing visuotactile stimulation to sustain the full body illusion during the CCT, which is too long for a previously induced illusion to be sustained otherwise.
In the two Body conditions in fact, participants have a 1PP over a highly collocated realistic virtual body, a condition that has been shown to be sufficient for experiencing a high sense of ownership toward the virtual body (Maselli and Slater, 2013). We then expected that the TT would be able to corroborate the sense of ownership by revealing an increase in the perceived separation between the legs at the end of both Body condition with respect to the baseline value: because participants remained in static posture throughout each experimental block, such an increase would be driven by the just seeing the virtual legs spreading apart and indeed would reveal the strong visuoproprioceptive coupling between the real and the virtual body that is established during ownership illusions. In summary, in order to validate point (3), the distance between the feet estimated through the TT should be larger than the baseline value in both Body conditions and should not vary in the control noBody condition, in which no visual cues about body posture was available.
Experiment 2 was designed to test the experimental hypotheses proposed in the introduction. The specific aim of the experiment was to show that it is possible to experience illusory changes in self-location, as in OBEs, with little if any illusion of ownership over the seen virtual body. At the same time, we expected that if ownership is induced over a dislocated body the perceived self-location should be strongly affected.
As in Experiment 1, we adopted a within-subjects design with three experimental conditions, in all of which the real and virtual bodies had the same posture. In 1PP-TO condition, participants saw the virtual body from a 1PP with a total overlap (TO) between the real and virtual bodies. In the 3PP-PO condition the visual perspective was shifted laterally with respect to the virtual body, while keeping a partial overlap (PO) between the two; the lateral shift was set ad-hoc for each participant so that the right leg of the avatar coincided in external space with the left leg of the participant (average later shift of 25 cm). In the third condition, the visual perspective was shifted laterally 80 cm to the right of the virtual body, with no overlap (NO). The three different visual perspectives over the virtual body are shown in Figure 2. In all the three conditions synchronous visuotactile stimulation was delivered symmetrically on both legs via the tracked tube, in exactly the same way as in Experiment 1. Note to the Body + VT condition of Experiment 1 and the 1PP-TO condition of Experiment 2 were exactly the same.
The procedure was the same as that described for Experiment 1. Participants went through all experimental blocks (one for each condition) after completing the training phase. Each block had an average duration of 10 min: the initial phase of tube's visuotactile stimulation (~1 min) was followed by the CCT (~8 min), a second phase of VT stimulation only (~1 min), the virtual legs spreading event (few seconds) and finally the TT. At the end of each block participants gave their scores to all questionnaire items while resting on the armchair.
The aim of the experiment was to test how ownership and self-location are modulated in FBI by shifting the visual perspective progressively away from the virtual body. Because we were interested in eliciting full body illusion we adopted the legs stroking as a trigger for the bodily illusions. The choice of the legs instead of, e.g., the chest, relays on several reasons: (i) first the legs and feet are visually prominent when looking down at our own body (if hands gesture is limited), particularly when laying in a resting posture; (ii) looking down at the chest requires a more targeted intention and is particularly uncomfortable in VR due to the HMD weight; (ii) stroking the legs allowed the stimulation of a wide portion of the whole body -from the feet to the thighs- and during the CCT to perform the stimulation while avoiding the region delimited by the CCT arrays; (iv) finally the legs separation could be easily manipulated without implying major changes in the overall resting posture.
Experiment 3 was planned and designed as a follow up of Experiment 2, because of the failure of the TT in assessing changes self-posture when combined with self-location assessments (see discussion in Section The “Ankles Pointing Task” (APT) and Discussion of the Results). A within-subject design was adopted with two conditions: the 1PP-TO and the 3PP-NO of Experiment 2. The procedure was exactly the same of Experiment 2 with two differences. First the APT substituted the TT. Second, before starting each experimental block, participants were outfitted with headphones streaming white noise. This latter modification was introduced to avoid the potential inhibitory effect that conflicting visuo-auditory cues may have in the 3PP condition, in which a spatial mismatch was present between the sound produced by the real tube stroking the participant's legs and the virtual tube's location on the virtual body.
The analysis reported in this Section was performed excluding outliers from the distributions under test; outliers were identified via the schematic boxplot introduced by Tukey (1977). The degrees of freedom reported for each test give information about the number of outliers excluded. Requirements for normality were always checked with the Shapiro-Wilk test (Shapiro and Wilk, 1965) and reported with its p-value (pSW). We avoided the debated practice of correcting for multiple comparisons (Perneger, 1998), and instead report effect sizes together with the true p-value for each performed test.
The average RT, EP and IE extracted from the CCT data and averaged across participants are given in Table 2 as a function of side, congruency, and condition, for all the three experiments.
An average of 11% of responses were given for no-go trials across participants, none of which exceeded the 30%. Figure 3A shows the bar chart for the full body CCE-IE. In all conditions the effect is larger when the LD is on the same side with respect to opposite. Three-Way ANOVA with interactions showed a significant main effect of congruency [F(1, 13) = 90.8, p < 0.0001, partial η2 = 0.39] and condition [F(2, 13) = 8.7, p = 0.0003, partial η2 = 0.11], and a significant interaction term between side and congruency [F(1, 13) = 24.4, p < 0.0001, partial η2 = 0.15]. Normality of the residuals was borderline (pSW = 0.053).
Figure 3. Results from Experiment 1. (A) Means and standard errors of the cross-modal congruency effect in inverse efficiency (CCE-IE) measured on the same side and on the opposite side, for the three experimental conditions (noBody, Body, and Body+VT). Ranges for the p-values from One-Way repeated measures ANOVA between same and other side are reported. (B) Differences (means ± standar errors) between the tube size estimation from the “Tube Task” (TT), perfomed after and before each of the experimental conditions: noBody, Body, and Body+VT. Positive values of ΔTubeSize indicate that participants experienced a recalibration of their perceived legs separation as a consequence of seeing the virtual legs spreading apart. Ranges for the p-values are reported for distributions with a mean significantly larger than zero (from one sample one-tailed t-test) and for paired comparisons across conditions (One-Way ANOVA). *0.01 < p < 0.05, **0.001 < p < 0.01, ***0.0001 < p < 0.001.
Pairwise comparisons of the CCE-IE between same and opposite side showed a significant difference for all conditions [noBody: F(1, 12) = 7.7, p = 0.017, partial η2 = 0.39; Body: F(1, 12) = 48.1, p = 0.0001, partial η2 = 0.80; Body + VT: F(1, 12) = 28.1, p = 0.0002, partial η2 = 0.72]. Residuals were normally distributed for all comparisons (pSW > 0.3).
“Tube Task” (TT)
Figure 3B shows the bar plot for the ΔTubeSize distributions in the three conditions. All were normally distributed (pSW > 0.85). ΔTubeSize is clearly larger than zero in the two Body conditions, as confirmed by one-sample one-tailed t-tests [Body: t(14) = 3.17, p = 0.003, Cohen's d = 0.82; Body + VT: t(14) = 2.36, p = 0.017, Cohen's d = 0.61]. This indicates a recalibration of the perceived posture. This was not the case for the noBody (control) condition. This result shows that, when the perceived posture was not affected by visual cues from the virtual body, participants reliably replicated the initial baseline estimation of their legs separation. It is important to stress that, despite de within-subjects design, the body posture's estimate did not retain aftereffects or biases from previous exposure to the conditions in which the virtual body was seen changing its posture.
One-Way repeated ANOVA revealed a significant effect of condition on ΔTubeSize [F(2, 14) = 6.5, p = 0.005, partial η2 = 0.26], with the residual being normally distributed (pSW = 0.24). Pairwise comparisons revealed that ΔTubeSize was significantly larger for both Body conditions with respect to the noBody condition [Body vs. noBody: t(14) = 3.4, p = 0.002, Cohen's d = 0.67; Body + VT vs. noBody: t(14) = 2.37, p = 0.016, Cohen's d = 0.53]. No difference between the two Body conditions was found [t(14) = 0.56, p = 0.58].
One subject was excluded from the analysis because he did not understand correctly the experiment instructions and another because the vibrators array stopped working during one of the conditions.
An average of 11.8% of responses were given for no-go trials across participants, none of which exceeded the 30%. Three-Way ANOVA with interactions revealed a significant main effect of congruency [F(1, 12) = 68.4, p < 0.0001, partial η2 = 0.34], a significant interaction term between side and congruency [F(1, 12) = 20.0, p < 0.0001, partial η2 = 0.13], and a trend for interaction of side, congruency and condition [F(1, 12) = 2.7, p = 0.071, partial η2 = 0.04]. The requirement for normality of the residuals was satisfied (pSW = 0.08).
Pairwise comparisons (Figure 4A) revealed a significantly higher CCE-IE on the same side with respect to opposite in 1PP-TO [F(1, 12) = 27.7, p = 0.0002, partial η2 = 0.70] and 3PP-PO [F(1, 12) = 74.2, p < 0.0001, partial η2 = 0.86], but not in 3PP-NO [F(1, 12) = 1.13, p = 0.31]. Residuals were normally distributed for all comparisons (pSW > 0.26). Because we expected a significant difference between the CCE on same and other side also in 3PP-NO, we repeated the test with the CCE computed for the RT data (CCE-RT). In this case we found CCE-RT to be higher in the same than in the other side [F(1, 14) = 4.57, p = 0.0507, η2 = 0.051, pSW = 0.17], in line with our expectations.
Figure 4. Results from Experiment 2. (A) Means and standard errors of the cross-modal congruency effect in inverse efficiency (CCE-IE) measured on the same side and on the opposite side, for the three experimental conditions (1PP-TO, 3PP-PO and 3PP-NO). The inset shows the same for the 3PP-PO subset of trials in which the target vibration was on the real left leg that overlapped in external space with the right virtual leg. Ranges for the p-values from One-Way repeated measures ANOVA between same and other side are reported. (B) Differences (means ± standar errors) between the tube lateral position set in the Tube Task (TT) after and before each experimental condition. Positive values of ΔTubeShift indicate that participants experienced a recalibration of their self-location to the left, which is toward the virtual body. Ranges for the p-values are reported for distributions with a mean significantly larger than zero (from one sample t-test) and for paired comparisons across conditions (One-Way ANOVA). (C) Differences (means ± standar errors) between the tube size estimation from the Tube Task (TT), perfomed after and before each experimental condition. The ΔTubeSize distributions match the zero mean distribution in all the three conditions. (D) Box plots showing the comparison of questionnaire data across conditions: only items for which significant differences among conditions have been found are shown. Ranges for the p-values from matched pairs Wilcoxon tests are reported. *0.01 < p < 0.05, **0.001 < p < 0.01, ***0.0001 < p < 0.001, ****0 < p < 0.0001.
It was interesting to specifically inspect performance on the 3PP-PO trials with the target vibration on the left leg of the participant. In fact, for these trials, the light distracters on the “opposite side” were located, in external space, in exactly the same positions of the vibrators (the virtual right leg overlapped in external space with the participant's left leg), while those on the “same side” were spatially incongruent. Despite this, the average CCE-IE was much higher on the same side than on the opposite (see inset in Figure 4A). A significant difference between same and opposite side was detected in this subsample with a matched-paired Wilcoxon test (z13 = 3.1, p13 = 0.002, PSdep = 0.92), as when running an One-Way ANOVA the resulting residuals were not normally distributed.
Due to technical recording problems, the TT data were missing from three subjects of the 15 subjects under analysis. The Shapiro Wilk test revealed normal distributions for both ΔTubeSize and ΔTubeShift (pSW > 0.24). Two outliers (one in 1PP-TO and one in 3PP-NO) were found and excluded from the analysis.
Figure 4B shows the bar chart for the ΔTubeShift distributions. For all conditions the distributions were normal (pSW > 0.20). One-sample one-tailed t-test analysis revealed a distribution mean significantly larger than zero for both the 3PP-PO [t(11) = 7.59, p < 0.0001, Cohen's d = 2.19] and 3PP-NO [t(10) = 2.8, p = 0.0097, d = 0.84], but not for 1PP-TO [t(10) = 1.5, p = 0.086, d = 0.44].
One-Way repeated ANOVA revealed a significant effect of condition on ΔTubeShift [F(2, 9) = 13.66, p = 0.0002, partial η2 = 0.60], with normally distributed residuals (pSW = 0.99). Pairwise comparisons revealed that ΔTubeShift was significantly larger in both 3PP conditions with respect to 1PP-TO [3PP-PO vs. 1PP-TO: t(10) = 5.4, p = 0.0002, Cohen's d = 2.62; 3PP-NO vs. 1PP-TO: t(9) = 2.2, p = 0.029, d = 0.92]. Interestingly, ΔTubeShift in the 3PP-PO condition was found to be significantly larger than in 3PP-NO [t(10) = 3.4, p = 0.0036, Cohen's d = 1.79].
The ΔTubeSize values were normally distributed in all conditions (pSW > 0.24). Contrary to our expectations the TT did not detect significant changes in the perceived posture for any of the conditions (see Figure 4C). One-sample t-test performed on the three distributions revealed indeed no difference from the zero-mean distribution (p > 0.29). One-Way repeated ANOVA also revealed no significant difference between conditions [F(2, 11) = 1.73, p = 0.2].
This unexpected outcome may be due to the interaction between the tube size adjustment and the additional lateral shift. In fact the 1PP-TO condition in this experiment is exactly the same as the Body + VT condition in Experiment 1, with the only difference being instructions given for the TT. The fact that in Experiment 1 the distribution of ΔTubeSize has a mean significantly larger than zero, while in Experiment 2 its mean is statistically equal to zero suggests that having the possibility to shift the tube laterally, and actually doing it, has a detrimental effect on the TT's measure of perceived body posture. The only difference between the two groups was in fact that in the latter participants had the possibility to shift the tube's laterally a part from adjusting its size.
Median values and interquartile ranges are reported in Table 3 for all items. The results from matched pairs Wilcoxon tests are given in Table 4 (items for which none of the three comparisons was significant were omitted). Effect sizes are given in terms of the probability of superiority of dependent measures, PSdep, defined as the probability that the score from the condition that most frequently has the higher score will be greater than the score from the condition that most frequently has the lower score (Grissom and Kim, 2012). The table highlights all significant comparisons as shaded cells. Boxplot corresponding to the significant comparisons are shown in Figure 4D.
Three-Way ANOVA with interactions revealed a significant main effect of congruency [F(1, 13) = 94.4.0, p < 0.0001, partial η2 = 0.51] and a significant interaction between side and congruency [F(1, 13) = 6.3, p = 0.014, partial η2 = 0.06]. Importantly, the three-way interaction between congruency, side and condition was not significant, indicating that visuotactile interactions were comparable in both conditions. The requirement for normality of the residuals was satisfied (pSW = 0.97). Pairwise comparisons between CCE-IE on same side and opposite side (shown in Figure 5A) revealed a significant difference in both the 1PP-TO [F(1, 15) = 15.7, p = 0.0012, partial η2 = 0.51] and the 3PP-NO [F(1, 15) = 5.79, p = 0.029, partial η2 = 0.28]. Residuals were normally distributed (pSW > 0.19).
Figure 5. Results from Experiment 3. (A) Means and standard errors of the cross-modal congruency effect in inverse efficiency (CCE-IE) measured on the same side and on the opposite side, for the two experimental conditions (1PP-TO and 3PP-NO). Ranges for the p-values from One-Way repeated measures ANOVA between same and other side are reported. (B) Box plot of the differences between the hands separation measured in the “Ankles Pointing Task,” after and before each experimental condition. Positive values of ΔHS indicate that participants experienced a recalibration of their perceived legs separation as a consequence of solely seeing the virtual legs spreading apart. ΔHS values were significantly larger in 1PP-TO than in 3PP-NO, showing that a proprioceptive recalibration occurred in the first condition but not in the latter. The p-value from matched pairs Wilcoxon test is reported. (C) ΔHS values are plotted against the scores to the mybody questionnaire item. A strong statistical trend (p = 0.055) for a positive correlation between the two variables was found. (D) Box plots showing the comparison of questionnaire data across conditions: only items for which significant differences among conditions have been found are shown. Ranges for the p-values from matched pairs Wilcoxon tests are reported. *0.01 < p < 0.05, **0.001 < p < 0.01, ***0.0001 < p < 0.001.
“Ankles Pointing Task” (APT)
Data from two subjects were missing due to recording problems. Another two data sets were discharged because of the participant having misinterpreted the task instructions. A total of 15 subjects were included in the analysis. It is notable that the ΔHS estimates from these two subjects were “extreme” outliers (Tukey, 1977) in the ΔHS distribution from 3PP-NO.
The ΔHS distribution extracted from the APT was not normal, but resembled a bimodal distribution with one cluster around zero and one another at positive values. This was particularly the case for the 1PP-TO distribution. The cluster around zero was probably a biased from participants that based their measure on the knowledge that their feet did not mov, as explicitly reported by one of the two excluded participants.
A matched-pairs Wilcoxon rank test revealed a significant difference between the two experimental conditions (z14 = 2.42, p14 = 0.016, PSdep = 0.71), indicating that participants perceived a significantly larger distance between their own ankles in the 1PP-TO than in the 3PP-NO. The box plot of the two distributions is shown in Figure 5B.
Questionnaire results are summarized in Table 5 and shown in Figure 5D. The results are consistent with those from Experiment 2, apart from the drift-left item. Scores were in fact significantly higher in 1PP-TO than in 3PP-NO for the items my-body (z = 3.27, p = 0.001, PSdep = 0.89) and the three items assessing the response to the legs separation event (legs-moving: z = 3.18, p = 0.001, PSdep = 0.86; legs-weird: z = 2.65, p = 0.008, PSdep = 0.78; legs-instinct: z = 2.29, p = 0.022, PSdep = 0.72). On the other hand, scores s-else were significantly higher in 3PP-NO than in 1PP-TO (z = 2.9, p = 0.004, PSdep = 0.78). Differently from Experiment 2, no significant difference was found for drift-left, although a weak trend for scores to be higher in 3PP-NO than in 1PP-TO was found (z = 1.52, p = 0.13). This was due to the extremely low scores given to this item in the 3PP-TO condition across all participants, which was not the case in Experiment 2.
Table 5. Experiment 3: Medians, interquartile ranges and paired comparisons of the questionnaire scores.
We tested the correlation between the level of ownership as assessed from questionnaire scores to the mybody item and the changes in the perceived legs separation measured with the APT (ΔHS). The Spearman's rho was showing a close to statistical significant positive correlation between the two measures (ρ = 0.34, p = 0.055), also shown in the scatter plot in Figure 5C. It is remarkable that despite the cognitive bias discussed above (participants knew that their feet did not move), which introduces extra variance in the APT data hindering correlation analysis, a positive correlation was found. This result further corroborates the validity of APT method as measure for ownership illusions.
Discussion of the Results
Results from Experiment 1 validated the methods used in the study. It was shown that the new proposed version of CCT, with the LEDs/vibrators arranged on the legs, is a valid tool to detect effective integration of visuotactile (VT) bodily signals, and thus to detect changes in the perceived self-location during full body illusions (see discussion in Section Materials and Methods). The CCT revealed in fact effective visuotactile interactions in both the Body conditions, in which participants experienced a fully collocated virtual body from a 1PP, a condition shown to be sufficient for eliciting a FBOI (Maselli and Slater, 2013). Importantly, the effective SOA was 20 ms, so that effective VT interactions can be primarily attributed to effective VT integration (Shore et al., 2006). In a similar setup, Aspell et al. (2009) had instead to increase the SOA to 233 ms in order to detect effective VT interactions when additional visuotactile stimulation was delivered during the CCT; however, as a drawback, the observed interaction was mainly dominated by exogenous attention rather than MSI (Shore et al., 2006). This difference may be due to the fact that, in our study, the visuotactile stimulation was delivered outside the region delimited by the CCT lights/vibrators array.
Interestingly, effective VT integration was detected also in the noBody condition. In previous studies, a similar condition was implemented, but with the array of visual distracters shifted with respect to the vibrators: in the “rubber hands absent” condition of Pavani et al. (2000) the vibrators were located on the unseen hands and the LEDs array was shifted 20 cm upward; similarly, in the “body not visible” condition of Aspell et al. (2009) the vibrators, located on the back while the distracters, were seen in the far extra-personal space. In both studies no significant visuotactile interaction was detected when the body was not visible. A significant CCE effect has instead been reported for an object condition in Salomon et al. (2012); however, as suggested by the authors, it is not straightforward to interpret this results because of the experimental design adopted, in which “object” trials where intermixed at a fast pace with “body” trials (Salomon et al., 2012). The fact that we did find evidence for visuotactile interaction also when the body is not visible is most probably due to the fact that LED distracters and vibrators were in spatial register. The result shows that the spatial information about the body provided solely by proprioception is sufficient to have a robust stored localization of body parts that contribute to the multisensory integration of visual and tactile information. This is consistent with the previous findings of the CCE modulation associated with various degree of visuo-proprioceptive spatial mismatch (Spence et al., 2008).
Experiment 1 further validated the TT/APT as novel tools for objective assessments of full body ownership illusions. The TT data showed that in the two Body conditions (in which ownership was experienced toward the virtual body) after seeing the virtual body moving the feet apart and getting into a new posture, participants estimated a larger distance between their feet even if the physical body did not move at all. This outcome relays, as expected, on the strong coupling of visual and proprioceptive cues from the virtual and real bodies that is established during an ownership illusion (see Section 4.4 in Maselli and Slater, 2013). In this respect, the validation of the TT as a tool to assess full body ownership through assessments of illusory changes in posture driven by movements of the virtual body only extends to the APT. In fact, in the latter differs from the TT only in the way in which participants indicated the perceived distance among their feet, which was design ad-hoc to provide estimates of the legs separation in a body centered reference frame. The validity of the APT as a proxy for the FBOI was further corroborated by the positive correlation found between ownership measures from questionnaire (scores to the mybody item) and the illusory change in body postures measured with the APT (ΔHS), found in Experiment 3.
Experiments 2 and 3
The outcome of Experiments 2 and 3 supports our main experimental hypotheses. We first discuss the set of results that show how ownership and self-location can be manipulated independently from each other, during FBOI and OBE illusion respectively. We do this by comparing data from the 1PP-NO and 3PP-NO conditions. We then discuss results that show how simultaneous changes in ownership and self-location can be induced, and do interact, when having a third person visual perspective of a virtual body that partially overlaps with the physical body, as in the 3PP-PO condition.
FBOI and OBE can occur independently of each other
As expected we found evidence for FBOI to occur in the 1PP-TO but not in the 3PP-NO in both questionnaire and APT data. Scores to the my-body item, as well as to all the three legs items, were significantly higher in 1PP-TO than in 3PP-NO, showing that ownership toward the virtual body was experienced consistently across participants, only in the 1PP-TO condition. The APT data from Experiment 3 corroborated this finding. A significantly larger change in the perceived feet separation was in fact found in the 1PP-TO condition with respect to 3PP-NO. This showed that a tight coupling between vision and proprioception, characteristic of ownership illusions, is established in the 1PP-TO but not in the 3PP-NO condition. In both Experiments 2 and 3, scores to the s-else item were significantly higher in 3PP-NO than in 1PP-TO. This complementary result shows that the virtual body seen in the far extra-personal space was experienced as the body of another person rather that as one's own body, supporting the finding that no ownership is generally experienced toward the virtual body seen in the far extra-personal space. Despite this, both TT and CCT performances consistently show that actual changes in the perceived self-location took place in 3PP-NO. The distribution of ΔTubeShift in 3PP was found to be significantly larger than zero (indicating a perceived lateral shift in the perceived position of the whole body) and significantly larger than the ΔTubeShift measured in 1PP-TO (which was compatible with no lateral shift). This finding is consistent with results from previous studies of experimentally induced OBEs, which measured similar shifts of the perceived self-location toward the virtual body using methods akin, e.g., the walking task (Lenggenhager et al., 2007) and the mental ball dropping task (Lenggenhager et al., 2009; Ionta et al., 2011). Notwithstanding the evidence provided by questionnaire and TT data for an altered self-location in 3PP-NO, the CCT data from Experiment 2 in this condition did not provide clear evidence for integration of visual cues on the virtual body and tactile cues from the correspondent body locations on the physical body (the CCE-IE was not significantly dependent on side). This was indeed contrary to our expectation. However, it is worth noting that a strong trend (p = 0.052) for a significant cross-modal interaction was found in the CCE-RT data (CCE measured on response times). Additionally, only a weak trend for a significant difference across conditions was found in the CCE-IE ANOVA (p = 0.07), which shows that the data do not provide clear evidence for CCT performance to be different in 3PP-NO than in the other two conditions, where a strong VT interaction was detected. The lack of a significant CCE-IE effect in 3PP-NO may be due to fact that visual distracters were far on the left side of the visual field, with a loss of sensitivity. In this case a larger sample would be needed. Also, conflictive visuo-auditory cues in 3PP-NO (from the tube stroking) may have acted as additional confounders making the task more difficult, and/or contributing negatively to the OBE illusion itself. These speculations are supported by CCT performances in Experiment 3, in which white noise was introduced to mask visuo-auditory incongruences on a larger sample of participants. A significant difference in the CCE-IE across same and different sides was indeed found in both 1PP-TO and 3PP-NO. This indicates that, during an OBE illusion, visual events experienced on the virtual body and tactile events experienced on the real body are integrated despite the dramatic spatial separation between them. It is important to notice that the feeling of another person to be present that may arise in our 3PP-NO condition (and generally in all experimental setups inducing full body illusion from a 3PP), may induce social mechanisms that affects self-processing (Serino et al., 2008), and in turns hinder the FBI. In any case, joint evidence from questionnaire (drift-left item), the CCT and the TT in the 3PP-NO condition speaks in favor of an actual shift of the perceived self-location toward the virtual body.
Summarizing, results from Experiments 2 and 3 provided the first experimental evidence that OBE and FBOI are two diverse perceptual illusions affecting different aspect of body self-consciousness. This was established by showing that both could occur independently of each other and that the two manifest themselves in different perceptual phenomena. In the general discussion we provide arguments for the different neural correlates that may be predominantly involved in each of the two illusions.
FBOI during OBEs: evidence for a strong interaction of ownership and self-location
Experiment 2 provided novel evidence on how the sense of ownership and the sense of self-location influence each other when they are concurrently manipulated. All data collected show that this was the case for the 3PP-PO condition, in which the visual perspective was dislocated from the eyes of the virtual avatar (3PP) while preserving a substantial overlap between the two bodies: participants in this condition experienced a strong FBOI over the virtual body with a concurrent significant change in the perceived self-location.
Apart from the drift-left item, questionnaire scores in this condition were statistically equal to those given in 1PP-TO. At the same time, the comparison between this condition and the 3PP-NO condition revealed the same significant differences found in the 1PP-TO vs. 3PP-NO comparison. This indicated that a strong FBOI occurred similarly in both 1PP-TO and 3PP-PO. On the other hand, TT data showed that participants experienced a strong drift in the perceived self-location. Furthermore, the drift was significantly stronger than that experienced in 3PP-NO, despite the fact that the actual displacement among the virtual and real bodies was much greater in the latter.
Analogously, the CCT data from the 3PP-PO condition support a significant shift in the perceived self-location. The highly significant cross-modal interaction found in 3PP-PO clearly showed that vibrotactile cues were indeed processed in an updated spatial register in which tactile events on the real body and visual events on the virtual body are associated according to a precise bodily-based topological correspondence. This result is even more remarkable when considering that, in this condition, the vibrotactile cues on the real left leg of participants were exactly collocated with the LEDs on the right leg of the virtual body, so that if no shift in the perceived self-location occurred we should have observed a strong interaction among these visuotactile cues, which was instead not observed (see inset in Figure 4B).
Summarizing, the experimental manipulation in the 3PP-PO condition induced concurrent changes in ownership and self-location. Most importantly, the latter was much stronger than the analogous change experienced in the 3PP-NO condition, indicating a strong interaction between ownership and self-location. Despite the fact that the two can be dissociated from each other, it seems that the sense of ownership experienced over a dislocated virtual body can importantly boost the experienced drift in the perceived self-location.
The experimental study here presented provides direct experimental evidence that the sense of ownership and the sense of self-location can be selectively manipulated in full body illusions. It was shown that it is possible to experience illusory changes in the perceived self-location during OBEs when no sense of ownership is experienced toward the virtual body. On the other during FBOI experienced toward a fully collocated virtual body, the perceived self-location was (obviously) not altered, as no conflict in the location of the two bodies was present.
The fact that OBE illusions can occur without changes in the sense of ownership and that OBE and FBOI are indeed two different perceptual illusions was previously suggested (Petkova et al., 2011b; Maselli and Slater, 2013), but to date it was not supported by experimental data.
Previous studies on OBE illusions have been primarily designed to manipulate and measure illusory changes in self-location. Despite this OBE illusions are often considered and discussed as belonging to the wider class of full body ownership illusions (e.g., Blanke, 2012; Limanowski et al., 2013). Having here shown that FBOI and OBE are different perceptual illusions is thus particularly important, because the lack of a clear distinction between the two illusions often resulted in interpreting observed changes in self-location as evidence for a sense ownership experienced toward a body seen in the far extra-personal space (Lenggenhager et al., 2007; Aspell et al., 2012).
As a second important outcome, the present study showed that when seeing a virtual body that overlaps only partially with the space occupied by the physical body, a strong FBOI is experience together with a significant shift in the perceived self-location toward the virtual body. This is indeed the first explicit experimental evidence for a FBOI experienced while having a third person visual perspective over a fake full body. Our data further show a positive interaction between the two components of body self-consciousness: in this “partial overlap” configuration, the experienced shift in self-location is in fact significantly larger than the one experienced in an OBE illusion. It seems then that, when both ownership and self-location are affected during a full body illusion, it is the sense of ownership that drives the sense of self-location toward the owned body.
Although our study does not provide any direct evidence for the existence of distinct neuronal correlates for FBOI and OBEs, in the following we propose plausible candidates for the sets of neuronal structures that are likely involved in the two illusions.
On the Neuronal Correlates of Experimentally Induced OBEs and Peripersonal Space
It has been recently proposed (Blanke, 2012) that the best neural candidates for encoding the perceived shift in self-location experienced during experimentally induced OBEs are the populations of bimodal visuotactile (VT) neurons hosted in cortical and subcortical areas of the primates brain.
Bimodal visuotactile populations are characterized by tactile receptive fields that can cover small patches or large portions of the body surface, and by visual receptive fields (vRF) anchored to the body part mapped in the tactile modality (Graziano and Gross, 1993; Duhamel et al., 1998; Brozzoli et al., 2012; Huang et al., 2012). For these characteristic properties bimodal VT populations have been suggested to encode the peripersonal space (Fogassi et al., 1996): through their vRFs these populations define in fact the region of space surrounding the body in which visual stimuli may trigger somatic sensations. The main functional role of this dynamic representation of the external space in body-parts centered coordinates is to guide movements for defensive purposes and for efficient interaction with external objects (Graziano and Cooke, 2006; Sereno and Huang, 2014).
Results from single cell recording in the monkey have further shown that the vRF of bimodal visuotactile neurons is highly plastic and can expand after the active manipulation of tools (Iriki et al., 1996). This groundbreaking result provided the first robust experimental evidence that the peripersonal space is highly plastic and can be reshaped in relatively short time scales. The plasticity of bimodal VT neurons has been then advocated as the mechanism that allows healthy humans to embody external objects, like tools, in their body schema (Maravita and Iriki, 2004).
As discussed thoroughly in Section The Cross-Modal Congruency Task as a Measure of Self-location, the cross-modal congruency task (CCT) has been shown to be a powerful psychophysical test to detect dynamic modulations of the peripersonal space during active tool use (Maravita et al., 2002), as well as during a rubber hand illusion (Pavani et al., 2000; Zopf et al., 2010). In line with these results, CCT performances in our study showed that, during experimentally induced OBEs, an actual extension (or shift) in the vRF of proximal visuotactile neurons occurs. This modulation can be interpreted as a modulation of the peripersonal space likely triggered by VT neural populations that maps large portions of the body, such as the legs. It is worth noting that neuronal populations with such properties have been identified in the human posterior parietal cortex (PPC) (Huang et al., 2012), and that the PPC has be shown to play a crucial role in mapping bodily signal into external space (Azañón et al., 2010).
In line with the original proposal from Blanke (2012), we argue that the dynamical changes in the vRF of visuotactile neurons with large receptive field on the body surface, is responsible for the perceived changes in self-location. We further argue that the same mechanisms may be regarded as the minimum common denominator of all OBEs. This proposal is not in conflict with the well-known implication of the TPJ in OBEs, which has been revealed in lesion analysis of patients suffering from recurrent OBEs (Blanke and Arzy, 2005), in electro-stimulation studies showing that OBEs can be systematically induced by direct stimulation of the TPJ (Blanke et al., 2002), and with fMRI performed during experimentally induced OBEs (Ionta et al., 2011). The latter study showed in fact that activity in the TPJ seems to encode the perceived elevation of the whole body -rather than its position in extra-personal space- and thus it is plausible that the TPJ implication in OBEs occurs at higher level in the hierarchical processing of sensory signals, in which a further integration is operated to combine visuotactile cues with vestibular and auditory information (Lopez et al., 2008).
Different Representations for the Sense of Ownership and Self-Location
Our study has shown that OBEs can occur with or without an associated ownership illusion. This implies that the dynamic changes in the peripersonal space associated with OBEs may be coupled or not with the activity of a larger and more complex network of neuronal populations that give rise to the sense of ownership during FBOI. Imaging studies have shown that the illusion of ownership for both body parts (RHI) and full bodies involves a complex network of brain areas that is still not well established and controversial. Depending on the experimental design and the imaging technique adopted, the ownership illusion has been found to correlate with activity in several brain areas: ventral premotor cortex (vPMc) and posterior parietal cortex (PPc) (Ehrsson et al., 2004; Petkova et al., 2011a), primary somatosensory cortex (Limanowski et al., 2013; Shokur et al., 2013), the extrastriate body area (EBA) (Limanowski et al., 2013); and, at subcortical level, the insula (Tsakiris et al., 2007; Limanowski et al., 2013). Recent theoretical accounts for body ownership have stressed the role of multimodal hierarchical processing of sensory and motor signals from the body (e.g., Hohwy, 2007; Ehrsson, 2011; Moseley et al., 2012; Limanowski and Blankenburg, 2013). Although a review of these works is beyond the scope of the present work, we want to stress here the potential contribution that our results may bring about.
The present study has highlighted, in particular, the fundamental role of visuo-proprioceptive correlations in driving FBOI. In a previous study we showed how congruent visuo-proprioceptive correlations from a (realistic) virtual body and the physical body could be sufficient to elicit a FBOI with no need for additional multimodal correlations (Maselli and Slater, 2013). The current study provides more stringent evidence for the effective coupling of visual signals from the virtual body and proprioceptive signals from the real one, that is established during FBOI. It was in fact shown that proprioceptive information about the body posture can be significantly modulated by seeing a change in the posture of the virtual body, even when the physical body does not move. Furthermore, we found that if visual and proprioceptive cues about the body are not in severe conflict (i.e., when a partial spatial overlap of the virtual and real bodies is preserved) a strong FBOI can be experienced. We additionally found that in this case the FBOI has the effect of strengthening the recalibration of tactile sensations toward the virtual body, which is characteristic of OBEs. These results strongly support the earlier proposals (Moseley et al., 2012; Maselli and Slater, 2013) suggesting that a fundamental node of the complex network of brain areas involved in ownership illusions is the neural population homologous to the one hosted in area 5 of the monkey parietal cortex, that integrates visual and proprioceptive signals to encode limb position (Graziano et al., 2000).
We have presented an experimental study conducted with the support of virtual reality, with the aim of exploring the relation between two of the important components of body self-consciousness: ownership and self-location. Our results provide direct experimental evidence that the sense of ownership and the sense of self-location can be selectively manipulated in experimentally induced full body illusions. It was shown that FBOIs and OBEs could occur independently of each other, affecting ownership and self-location respectively. It was also shown that the two illusions could co-occur affecting concurrently ownership and self-location; our results suggest that in these cases illusory changes in ownership and self-location have a synergic interaction.
We discussed how our results support the recent proposal suggesting that self-location is strictly related with a reshaping of the visual receptive field of proximal visuotactile neurons mapping large portions of the body's surface. On the other hand, we discussed how our results support the need for a driving activation of visuo-proprioceptive neuronal populations in full body ownership illusions.
Finally, our results moderate previous conclusions about the role of the visual perspective in ownership illusions. It was in fact shown that a FBOI could be experienced from a 3PP when the virtual body preserved a partial spatial overlap with the physical body.
Conflict of Interest Statement
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential.
We thank Sofia Osimo and David Martínez Rubio for their help in running the experiments. This study was funded by the European Research Council, within the project TRAVERSE (Contract 227985).
The Supplementary Material for this article can be found online at:http://www.frontiersin.org/journal/10.3389/fnhum.2014.00693/abstract
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Keywords: ownership, self-location, full body ownership illusions, out of body experience, multisensory integration, visual perspective, cross-modal congruency effect, virtual reality
Citation: Maselli A and Slater M (2014) Sliding perspectives: dissociating ownership from self-location during full body illusions in virtual reality. Front. Hum. Neurosci. 8:693. doi: 10.3389/fnhum.2014.00693
Received: 04 April 2014; Accepted: 19 August 2014;
Published online: 11 September 2014.
Edited by:Olaf Blanke, Ecole Polytechnique Fédérale de Lausanne, Switzerland
Reviewed by:Ilaria Bufalari, Sapienza University of Roma, Italy
Roy Salomon, Ecole Polytechnique Fédérale de Lausanne, Switzerland
Copyright © 2014 Maselli and Slater. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Antonella Maselli and Mel Slater, EVENT Lab, Facultat de Psicologia, Universitat de Barcelona, Passeig de la Vall d'Hebron 171, Barcelona 08035, Spain e-mail: email@example.com; firstname.lastname@example.org