Considerations for measuring activity-dependence of recruitment of synaptic vesicles to the readily releasable pool.
- 1Institute of Neurosciences of Alicante (IN), Spain
The connection strength of most chemical synapses changes dynamically during normal use as a function of the recent history of activity. The phenomenon is known as short-term synaptic plasticity or synaptic dynamics, and is thought to be involved in processing and filtering information as it is transmitted across the synaptic cleft. Multiple presynaptic mechanisms have been implicated, but large gaps remain in our understanding of how the mechanisms are modulated and how they interact. One important factor is the timing of recruitment of synaptic vesicles to a readily-releasable pool. A number of studies have concluded that activity and/or residual Ca2+ can accelerate the mechanism, but alternative explanations for some of the evidence have emerged. Here I review the methodology that we have developed for isolating the recruitment and the dependence on activity from other kinds of mechanisms that are activated concurrently.
Keywords: RRP, readily-releasable, residual, activity, recruitment, Vesicle pool
Received: 05 Jul 2019;
Accepted: 06 Nov 2019.
Copyright: © 2019 Wesseling. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Prof. John F. Wesseling, Institute of Neurosciences of Alicante (IN), San Juan de Alicante, Spain, firstname.lastname@example.org