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Ion Transport in Cell Cycle and Cancer

Original Research ARTICLE

Front. Physiol., 14 August 2013 | https://doi.org/10.3389/fphys.2013.00201

PIST (GOPC) modulates the oncogenic voltage-gated potassium channel KV10.1

Solveig Herrmann1, Milena Ninkovic2†, Tobias Kohl2† and Luis A. Pardo1*
  • 1AG Oncophysiology, Max-Planck Institute of Experimental Medicine, Göttingen, Germany
  • 2Department of Molecular Biology of Neuronal Signals, Max-Planck Institute of Experimental Medicine, Göttingen, Germany

Although crucial for their correct function, the mechanisms controlling surface expression of ion channels are poorly understood. In the case of the voltage-gated potassium channel KV10.1, this is determinant not only for its physiological function in brain, but also for its pathophysiology in tumors and possible use as a therapeutic target. The Golgi resident protein PIST binds several membrane proteins, thereby modulating their expression. Here we describe a PDZ domain-mediated interaction of KV10.1 and PIST, which enhances surface levels of KV10.1. The functional, but not the physical interaction of both proteins is dependent on the coiled-coil and PDZ domains of PIST; insertion of eight amino acids in the coiled-coil domain to render the neural form of PIST (nPIST) and the corresponding short isoform in an as-of-yet unknown form abolishes the effect. In addition, two new isoforms of PIST (sPIST and nsPIST) lacking nearly the complete PDZ domain were cloned and shown to be ubiquitously expressed. PIST and KV10.1 co-precipitate from native and expression systems. nPIST also showed interaction, but did not alter the functional expression of the channel. We could not document physical interaction between KV10.1 and sPIST, but it reduced KV10.1 functional expression in a dominant-negative manner. nsPIST showed weak physical interaction and no functional effect on KV10.1. We propose these isoforms to work as modulators of PIST function via regulating the binding on interaction partners.

Introduction

The voltage gated potassium channel KV10.1 is the founding member of the family of ether à go-go channels (Warmke and Ganetzki, 1994). The function of this ion channel in the central nervous system, where it is normally exclusively expressed, still remains elusive (Occhiodoro et al., 1998; Pardo et al., 1999). Ectopically expressed KV10.1 induces a transformed phenotype, and the channel is expressed in 70% extra-cranial tumors (Hemmerlein et al., 2006; Mello De Queiroz et al., 2006; Ding et al., 2007a,b, 2008; Ousingsawat et al., 2007; Wadhwa et al., 2009; Agarwal et al., 2010; Asher et al., 2010). Although there is evidence pointing to an additional non-canonical, permeation-independent role of the channel in cancer initiation and growth (Downie et al., 2008; Chen et al., 2011), inhibition of channel function exclusively at the plasma membrane by a monoclonal antibody (Gomez-Varela et al., 2007) reduces tumor progression in vivo, indicating that the membrane population of the channel is very important for its oncogenic properties. However, the mechanisms regulating the expression of KV10.1 at the surface are still unclear, although it is known that the membrane residence time of the channel is relatively short, because it is internalized at a rate of 2% per minute (Kohl et al., 2011), and that both rabaptin 5 and cortactin are important to determine the abundance of KV10.1 at the plasma membrane at a given time(Herrmann et al., 2012; Ninkovic et al., 2012).

PIST (PDZ domain protein interacting specifically with TC10), also called GOPC (Golgi-associated PDZ and coiled-coil motif-containing protein), CAL (CFTR-associated ligand) or FIG (fused in glioblastoma) was first described in 2001 as a coiled-coil and PDZ domain-containing protein that specifically interacts with the GTPase TC10 (Neudauer et al., 2001). Depending on one of its coiled-coil regions, PIST mainly resides in the Golgi apparatus, but can also co-localize with its binding partners at the plasma membrane (Charest et al., 2001; Yao et al., 2001; Wente et al., 2005b). Many studies have addressed the possible interaction partners of PIST, in order to unravel the function of this protein. Proteins known to be located at the trans-Golgi network (TGN) like Syntaxin 6 or Golgin160 are able to bind the coiled-coil region of PIST, pointing to an involvement of this protein in processes like TGN sorting and vesicle trafficking (Charest et al., 2001; Hicks and Machamer, 2005). A crystal structure of this domain of PIST is available (Shin et al., 2013). The ability of PIST to bind activated small GTPases like TC10 and Rab6a may serve as a mechanism controlling PIST function in response to signaling pathways, as shown for cystic fibrosis transmembrane conductance regulator (CFTR) (Neudauer et al., 2001; Cheng et al., 2005; Bergbrede et al., 2009). Most other interaction partners of PIST are reported to bind to its PDZ domain, among them CFTR itself (Cheng et al., 2002), the G-protein-coupled receptors frizzled 5 and 8 (Yao et al., 2001), the chloride channel CIC-3B (Gentzsch et al., 2003), the β 1-adrenergic receptor (He et al., 2004), the somatostatin receptor subtype 5 [SSTR5 (Wente et al., 2005b)], a metabotropic glutamate receptor [mGluR1a, (Zhang et al., 2008)] and Cadherin 23 (Xu et al., 2010). Several studies discuss that overexpression of PIST might lead to different surface expression patterns of some of these membrane proteins by holding them back in the Golgi (He et al., 2004; Wente et al., 2005b; Xu et al., 2010). A different and more detailed process is postulated for CFTR, where PIST enhances degradation by facilitating its targeting to lysosomes (Cheng et al., 2005). A fusion between PIST and the proto-oncogene ROS1 has been detected, initially in glioblastoma and subsequently in other cancer types (Charest et al., 2003; Birch et al., 2011; Gu et al., 2011; Suehara et al., 2012).

Relevant functions of PIST in the brain appear to be mediated by an alternatively spliced isoform (nPIST) that contains an eight amino acid insertion in the second coiled-coil region (Yue et al., 2002). This isoform is able to interact with Beclin1 over its coiled-coil domain and therefore was linked to autophagy in neurons of lurcher mice (Yue et al., 2002), although constitutive ion fluxes were able to induce cell death regardless of the nPIST-Beclin1 interaction (Nishiyama et al., 2010). Additionally, glutamate receptors are able to bind to the PDZ domain of nPIST over their extreme C-terminus (Yue et al., 2002; Cuadra et al., 2004). This interaction in concert with Stargazin has been shown to enhance synaptic clustering of AMPA receptors in hippocampal neurons (Cuadra et al., 2004).

In this study, we identified PIST as a new interaction partner of KV10.1. We show physical as well as functional interaction between PIST and KV10.1. We were also able to identify and clone two new isoforms of PIST, and provide evidence that the four isoforms of PIST differ in terms of binding to and regulation of KV10.1.

Materials and Methods

Yeast Two-Hybrid

The yeast reporter strain L40 (Vojtek et al., 1993) (MATa, trp1, leu2, his3, LYS::lexA-HIS3, URA3::lexA-lacZ) was transformed with pLexN-hEag1 by the lithium acetate method and grown on synthetic medium lacking tryptophan. After additional transformation with plasmid pVP16-3-cDNA [postnatal 8 rat brain cDNA library, (Okamoto and Sudhof, 1997)], double transformants were plated on synthetic medium lacking histidine, leucine, uracil, lysine, tryptophan and in the presence of the competitive inhibitor of the HIS3 protein 3-amino-1,2,4-triazole (3-AT). Positive colonies were picked after 4–6 days and tested for ß-galactosidase activity using plate assay. Plasmids from positive clones were rescued and transformed in E. coli strain HB101. E. coli cells were plated on leucine-lacking medium. Positive clones were further analyzed by yeast retransformation and DNA sequencing.

RNA Purification, cRNA Synthesis and RT-PCR

HEK 293 cells were washed 3 times with ice cold PBS, harvested and directly used for RNA purification using RNAeasy Mini Kit (Qiagen). First strand cDNA was produced using SuperScript (Invitrogen) with oligo-dT. For cloning, PIST was amplified in a standard PCR reaction using Taq DNA polymerase (NEB) and sense and antisense primers (see below). PCR products were analyzed by electropohoresis, fragments excised out of gel and purified using NucleoSpin Extract (Macherey-Nagel) and subcloned in pGEMT-easy (Promega) following manufacturer's instructions.

Mouse first strand cDNA was a generous gift from Dr. R. Ufartes (Ufartes et al., 2013).

To distinguish between isoforms, PCR products were obtained in a standard PCR procedure using 100 ng cDNA mouse first strand cDNA, and a set of 2 or 3 specific primers in the appropriate combinations to distinguish between isoforms (see below) and Biotherme Polymerase (Genecraft).

Primers for human PIST (accession numbers NM_001017408.2—PIST- and NM_020399.3—sPIST-) were:

5′ ATGTCGGCGGGCGGTCCATGC3′ and

5′TTAATAAGATTTTTTATGATACAGAG3′

For mouse PIST (accession numbers NM_001199272.1 and NM_053187.3):

5′GCAGAGGGCGCAACGACTT3′ and

5′ATTCTCATGCGCATCCCTCACTG3′

For selective amplification of the previously described transcript variants, we used different forward primers (nPIST; NM_020399.3: 5′ CAAGGCAAAATTGTCTGTCCAC 3′; PIST, NM_001017408.2: 5′ CAAGGCAAAATTGGAAAGAGAAC 3′), and to distinguish between short and long forms, the following antisense primers were used:

Short 5′ CTCTCTGCTGGGAGAGTATAG 3′

Long 5′ CTCTCCTCTCTGTGTGATAGA 3′

Cell Culture and Transfection

HeLa cells were cultured in MEM+GlutaMax (Invitrogen) supplemented with 10% FCS (PAA), HEK293 cells in DMEM/F12+GlutaMax (Invitrogen) supplemented with 10% FCS and for stable cell line HEK293-KV10.1-BBS [HEK-BBS, (Kohl et al., 2011)] with Zeocin (Invitrogen) at 5% CO2 and 37°C.

Transfection was performed using Lipofectamine 2000 or Lipofectamine (Invitrogen) according to the manufacturer's instructions. KV10.x-BBS Venus and CFP-PIST were generated by cloning into pcDNA3 or pECFP-N1 vectors. Empty vectors were used as controls.

Fractional Labeling, Quantification and Purification of KV10.1-BBS

Labeling of whole-cell KV10.1-BBS was performed in cell lysates in buffer LP [20 mM Tris-HCl pH 7.4, 150 mM NaCl, 5 mM MgCl2, 1% Nonidet P40, protease inhibitors (Roche)] with α-bungarotoxin (BTX)-biotin conjugate (Invitrogen) in a final concentration of 0.2 μg/ml for 30 min on ice. To detect membrane and/or internalized KV10.1-BBS, transfected HEK-BBS cells were incubated in media supplemented with α-BTX-biotin conjugate (Invitrogen) in a final concentration of 2.5 μg/ml and kept at room temperature for 10 min (membrane) or at 37°C for one hour (internalized). For internalized KV10.1-BBS, cells were washed with ice-cold acid wash buffer (150 mM NaCl, pH 3.0) for 3 min to remove membrane labeling of KV10.1-BBS. Twice washing with cold PBS removed residual α-BTX-biotin conjugate. Cells were then harvested and lysed with LP buffer for 20 min on ice. Insoluble fraction was removed by centrifugation at 18,000 × g at 4°C, and the supernatant used for ELISA or pull down experiments.

For pull down approaches, labeled KV10.1-BBS was bound on streptavidin-coated magnetic beads (T1, Invitrogen) for 2 h at 4°C. Unbound protein was removed by washing 5 times with buffer LP supplemented with protease inhibitors (Roche). Bound protein was eluted at 70°C for 10 min using LDS sample buffer containing reducing agent (Invitrogen) and analyzed by SDS PAGE (Invitrogen) and western blotting.

Quantification of the amount of labeled KV10.1-BBS was performed by ELISA. After labeling, total cell lysates (30 and 150 μg protein), were immobilized on streptavidin-coated plates (Pierce) and detected using a C-terminal monoclonal anti-KV10.1 antibody [Ab33, 5 μg/ml (Hemmerlein et al., 2006)] and a polyclonal anti-mouse secondary antibody (Pierce, 1:500) coupled to peroxidase. ABTS (Invitrogen) was used as a substrate for development and detected in a Wallac Victor2 reader at 405 nm (reference 490 nm). Experiments were performed in duplicates.

Pull Down Experiments

For immunoprecipitation, rat brain lysates (800 μg total protein) were incubated overnight at 4°C with 2–5 μg of antibody [anti-KV10.1 33/62 (Hemmerlein et al., 2006); anti-PIST (Millipore); non-specific mouse IgGκ 2b) in buffer A (0,5% Triton X-100, 25 mM TrisHCl pH7.5, 75 mM NaCl, 2.5 mM EDTA and protease inhibitors (Roche)]. Pull down was performed by adding 20 μl of Protein G/A-coated Sepharose beads (Calbiochem) for 2 h at 4°C under rotation. After washing five times with buffer A, bound protein was eluted at 70°C for 10 min using LDS sample buffer containing reducing agent (Invitrogen) and analyzed by SDS PAGE (Invitrogen) and western blotting.

Electrophysiology

Recordings on Xenopus oocytes were performed as described by Stuhmer (1998) using a Turbo TEC-10CD amplifier (NPI electronics). cRNA was synthesized using the mMessage mMachine kit (Ambion) according to the manufacturer's instructions. 0.1–1 ng cRNA per oocyte were microinjected 1–3 days prior to recording. Cells were kept at 18°C in ND 96 solution (96 mM NaCl, 2 mM KCl, 0.2 mM CaCl2, 2 mM MgCl2, 5 mM Hepes/NaOH, pH 7.5) supplemented with theophylline (0.5 mM) to avoid maturation of the oocytes. For voltage clamp recordings, pipettes had resistances ranging from 0.5 to 1.2 MΩ when filled with 2 M KCl. External solution (NFR) contained 115 mM NaCl, 2.5 mM KCl, 1.8 mM CaCl2, 10 mM Hepes/NaOH, pH 7.2. Current was digitized at 5 kHz and filtered at 1 kHz. Currents were elicited by 1 s depolarizations from a holding potential of −100 mV to values ranging from +80 to −100 mV. Current amplitude was determined as mean value in the last 200 ms of the depolarization. To fit current-voltage relationships, we used an equation of the form:

I(V)=Γ·(VVrev)(1+eV50Vk)4

where Γ is the total conductance, Vrev the reversal potential, V50 the potential of half activation per subunit and k the slope factor.

Data acquisition and analysis was performed with Pulse-PulseFit (HEKA Electronics) and IgorPro (WaveMetrics).

Microscopy

For confocal microscopy, HeLa cells were grown on glass coverslips and transfected with Venus-tagged KV10.1 and CFP-tagged isoforms of PIST. Empty vectors served as control. 24 h after transfection, the Golgi marker BODIPY FL C5 Ceramide (Invitrogen) was used according to manufacturer's instructions to visualize Golgi apparatus. Cells were fixed in 4% paraformaldehyde in PBS for 10 min at room temperature, washed thoroughly with HBBS and mounted with ProLong Gold Antifade Reagent (Invitrogen). Image acquisition was performed using a Zeiss LSM 510 Meta confocal microscope with a Zeiss 40x 1.2 Korr water immersion objective. Single channel gray scale images are presented using color tables according to the fluorophore used. Merged images were generated by combination of the channel images into a single RGB file. Only linear contrast enhancement and Gaussian blur were applied off-line to the images; analysis was carried out with Fiji (Schindelin et al., 2012).

Results

PIST Interacts with the Voltage-Gated Ion Channel KV10.1

Finding new interaction partners of KV10.1is crucial to unravel the role of this ion channel both in the healthy brain and in tumors. To identify candidate interaction partners of KV10.1, we performed yeast two-hybrid screens using a rat brain expression library. The C-terminus of KV10.1 in fusion to the DNA binding domain of LexA transcription factor was used as a bait to screen a rat brain cDNA library fused to the activation domain of VP16 transcription factor. As previously reported (Ninkovic et al., 2012), out of 2 million screened clones, eight were positive for the HIS3 marker. One of them encoded most of the open reading frame of the murine PIST, including its PDZ domain (Neudauer et al., 2001).

We then performed co-immunoprecipitation studies to confirm the interaction. Precipitation of PIST using a specific antibody was able to pull down KV10.1 out of rat brain lysates (Figure 1A). In reverse co-IP experiments, PIST co-precipitated KV10.1 (Figure 1B). Non-specific isotype antibodies did not precipitate either of the two proteins.

FIGURE 1
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Figure 1. Co-precipitation of KV10.1 and PIST out of (A,B) rat brain lysates and (C) HEK293_KV10.1_BBS cells. Specific antibodies against PIST (A) or KV10.1 (B) were used for precipitation out of rat brain lysates using Protein A/G coupled sepharose beads. Isotype control antibodies served as negative controls. Co-precipitation of Kv10.1 by anti PIST immunoprecipitation (A) as well as precipitation of PIST by anti KV10.1 immunoprecipitation (B) were detected by western blot. (C) KV10.1_BBS was labeled with an α-bungarotoxin-biotin conjugate after lysis of HEK293_KV10.1_BBS cells and pulled down with streptavidin-coated beads. Detection on a western blot with and anti-PIST antibody shows bands compatible with precipitation of both CFP-tagged and endogenous PIST.

In a second pull-down approach we used HEK293 cells stably expressing a modified KV10.1 bearing an α-bungarotoxin (BTX) binding site [KV10.1_BBS, (Kohl et al., 2011)]. Specific binding of a BTX-biotin conjugate to its binding site allows precipitation of KV10.1_BBS from cell lysates. After transfection with CFP-labeled PIST, pull down of KV10.1_BBS with BTX-biotin and immunoblotting using anti-PIST antibodies revealed two bands compatible with endogenous PIST as well as CFP-labeled PIST (Figure 1C, lane +Bb); no bands were detected if BTX-biotin was omitted (Figure 1C, lane −Bb). The fact that both endogenous and CFP-tagged PIST are detected argues in favor of the specificity of PIST detection. As a control, the same experiments using unlabeled KV10.1_BBS did not precipitate PIST, also indicating specificity of the co-IP. Taken together, these results strongly indicate that PIST and KV10.1 interact physically both in vitro and in vivo.

Two New Short PIST Isoforms Lacking the PDZ Domain are Expressed in All Tissues Tested

For further analysis we amplified and cloned the human PIST out of cDNA obtained from untransfected HEK293 cells, using 5′ and 3′ flanking primers. Interestingly, during the cloning process we systematically observed two bands, one of them at the expected size of full length PIST and a shorter one. Cloning and subsequent sequencing of the shorter band revealed that it corresponds to a PIST variant lacking the complete exon 6, which is the region coding for the majority of the PDZ domain indicating that this clone corresponds to a new splice variant of PIST. To further proof the existence of the new isoform, we designed primers to generate an amplicon containing the skipped exon, in order to discriminate between the longer and the shorter form of PIST. We were able to amplify fragments with the expected sizes out of cDNA from HEK293 cells (data not shown). To evaluate the tissue distribution of the splice variant we used mouse cRNA from different tissues. The variant (lacking in the mouse exon 5) was detected in all tissues tested except pancreas, where amplification of full-length PIST was also much weaker (Figure 2A). The signals for the short form were consistently weaker than those for full-length PIST.

FIGURE 2
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Figure 2. New PIST isoforms. (A) PCR on cDNA from different mouse tissues show amplification of short (205 bp) and a long (408 bp) fragments corresponding to sPIST and PIST. (B) A PCR designed to differentiate neuronal and non-neuronal forms of PIST detect both short and long bands revealing the widely extended expression of both forms. (C) Schematic representation of the domains of all four PIST isoforms. Coiled-coil regions are depicted in orange, the insertion in n-PISTs in the second coiled-coil is represented in blue. The PDZ domain is represented in green, and a vertical line represents the site of deletion of exon 6.

In 2002, a second isoform of PIST was cloned and termed “neuronal PIST” (nPIST) because it is believed to be the main form in the brain. It differs from PIST by an 8 amino acid insertion in the coiled-coil region (Yue et al., 2002). The sequence we identified here codes for a protein that lacks the main part of the PDZ domain but bears the 8 amino acids of the neuronal form. We therefore asked if there is a fourth isoform, lacking the PDZ as well as the 8 amino acid insertion. PCR with primer pairs distinguishing between nPIST and PIST and producing different fragment length depending on the presence of the alternatively skipped exon was used to answer this question. Amplification of the expected fragments out of cDNA from HEK293 cells as well as from different murine tissues indicated that all four isoforms are expressed in nearly every tissue (Figure 2B). Weak or absent expression of nPIST in liver and pancreas correlated with no detection of the short form (nsPIST) of it, indicating that expression of the shorter form depends on the expression of the corresponding longer one (Figure 2B). The cartoon in Figure 2C depicts the domain structure of the four PIST isoforms. Taken together, our data suggests that two new short forms of PIST (from here on termed sPIST and nsPIST) exist and are expressed in nearly every tissue. According to our semi-quantitative approach, both seem to be less abundant than their corresponding longer form. sPIST and nsPIST sequences have been deposited under accession numbers KF420122 and KF420123, respectively.

Short and Long Isoforms of PIST have Different Capabilities for Binding ToKV10.1

PIST is known to form homodimers depending on the second coiled-coil motif, which bears also a leucine zipper (Neudauer et al., 2001). As this region is intact in every identified isoform, we tested if the shorter variants can form heterodimers with the longer ones. To this end, we transfected CFP-labeled PIST constructs and used a GFP-specific antibody to immunoprecipitate the extracts that were subsequently blotted using a PIST-antibody. Precipitation of CFP-tagged PIST and nPIST co-precipitated a band compatible with endogenous PIST (or nPIST), strongly suggesting that dimerization of PIST occurs in our system (Figure 3A). The major band that co-immunoprecipitated with CFP-tagged nsPIST was smaller than the one pulled by full-length forms and compatible with the size of an endogenous short isoform, suggesting that the short isoforms (or at least nsPIST) do occur at the protein level in vivo. Expression of sPIST-CFP was not clearly detectable even after immunoprecipitation, rendering interpretation of the results difficult with this isoform, even though an endogenous band was detected. We therefore excluded sPIST from transfection-based experiments and concentrated on the other three variants.

FIGURE 3
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Figure 3. (A) Heteromerization of long and short forms of PIST. (A) GFP antibody was used to precipitate CFP-tagged PIST isoforms out of lysates of transfected HEK293 cells. PIST isoforms were detected using a polyclonal antibody against PIST. Bands compatible with both the GFP-tagged forms and with endogenous PIST were detected, indicating association between the transfected CFP-PIST and the endogenous one. (B) Co-precipitation of PIST isoforms with KV10.1_BBS. Lysates of KV10.1_BBS-expressing cells were labeled with an α-BTX-biotin conjugate and pulled down with streptavidin-coated beads (see Figure 1). Anti-PIST specific antibodies detected co-immunoprecipitation of bands compatible with the corresponding CFP-tagged form, together with an endogenous PIST band.

We cannot exclude formation of heterodimers between nPIST and PIST isoforms, because HEK293 cells express all four isoforms of PIST and in this approach we cannot resolve the eight residues difference between PISTs and nPISTs.

We next tested the different isoforms for their capability to bind KV10.1. Precipitation of KV10.1_BBS out of stably expressing HEK293 cells was able to co-precipitate over-expressed CFP-tagged PIST and nPIST (Figure 3B). In comparison to the long forms, co-precipitation of the tagged short isoform CFP_nsPIST was much less evident in this assay (Figure 3B). Since the short forms lack most of the PDZ domain, these data suggest an involvement of the PDZ domain of PIST in KV10.1 binding. Interestingly, overexpression of the long isoforms resulted in higher intensity in bands compatible with the endogenous full-length PIST, while the shorter isoform rendered more intense bands corresponding to short isoforms (Figure 3B).

PIST Influences Currents Mediated by KV10.1, but not by KV10.2

We next tested if there are functional consequences of the interaction between PIST and KV10.1. As a model we used voltage clamp recordings in the Xenopus oocyte expression system and checked if the overexpression of any PIST isoform can influence KV10.1-mediated current. Regardless of the concomitant overexpression of human PIST isoforms, macroscopic KV10.1 currents showed very similar voltage dependence (Figure 4); in fact, the current-voltage relationship could be fitted using the same parameters used for KV10.1 alone (Figure 4B). We did not appreciate differences in kinetics, and the main properties of KV10.1 were conserved in the presence of PIST isoforms (not shown).

FIGURE 4
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Figure 4. PIST influences KV10.1 function. (A) Current-voltage relationship of KV10.1 expressed in Xenopus oocytes with or without PIST. Current amplitudes were significantly larger in oocytes coinjected with PIST (N = 4; **p < 0.01; ***p < 0.001). (B) After normalization to the current value at +100 mV, the current-voltage plots overlap, indicating that the voltage-dependence of the channel is not altered by PIST. (C) Relative current amplitude (at + 80 mV) in oocytes co-expressing PIST isoforms with respect to KV10.1 alone (N = 19). PIST (N = 18) induced a significant increase (p < 0.05), while nPIST (N = 10) and nsPIST (n = 14) did not alter current amplitude. (D) Co-injection of sPIST reduced the current amplitude both in the absence (N = 8) and in the presence (N = 8) of PIST. (E) PIST expression did not affect KV10.2 current amplitude.

The total current amplitude, however, was influenced by PIST expression (Figures 4A,C). Over-expression of PIST was able to nearly double the amplitude of KV10.1 mediated current (189 ± 33% of control levels). The difference was more significant the more depolarized the stimulus (reaching p < 0.0001 at +80 mV and above). Injection of nPIST and nsPIST did not affect the amplitude. These observations are compatible with an influence of PIST on the membrane expression of KV10.1, similar to the one described for CFTR (Cheng et al., 2005). In the presence of PDZ and coiled-coil domain, the amplitude of KV10.1 currents was increased. We observed no functional interaction with the insertion in the coiled-coil of “n” forms.

We previously stated that very poor and irregular expression of sPIST did not allow us to document physical interaction between this isoform and KV10.1. However, since experiments in oocytes do not depend on transfection or transcription, which is performed in vitro previous to oocyte injection, we also tested if injection of synthetic cRNA encoding for sPIST affects the amplitude of the KV10.1-mediated current. Surprisingly, sPIST coinjection induced a reduction in current amplitude to 55 ± 4% of control levels. Although they had opposite effects, coinjection of a mixture of PIST and sPIST together with KV10.1, did not results on compensation of the effects, but rather sPIST showed a dominant-negative effect, in such a way that the current was reduced to the same extent as when sPIST was injected alone (Figure 4D).

A Golgi-resident protein like PIST might have a general effect on the expression level of ion channels in the oocyte expression system, enhancing transport mechanism toward the membrane. As a measure for the specificity of our observations, we tested the effect of PIST overexpression on KV10.2, the closest relative of KV10.1. PIST overexpression did not change voltage-dependence or kinetic parameters of KV10.2. In contrast to KV10.1, there was also no change in current amplitudes of KV10.2-mediated currents (Figure 4E), pointing toward a highly specific effect of PIST expression on KV10.1, but not KV10.2 current amplitude.

Cellular Distribution of KV10.1 Changes in Response to PIST Overexpression

Having shown that PIST is able to interact physically and functionally with KV10.1, we next asked if both proteins co-localize in vivo. PIST is reported to reside mainly in the Golgi apparatus, depending on at least one coiled-coil region, but can also be pulled to the membrane in co-expression with its interaction partners (Charest et al., 2001; Yao et al., 2001; Wente et al., 2005b). To perform subcellular localization studies, we used CFP-tagged forms of PIST. As described in the literature, the longer forms of PIST were mainly located in the Golgi, the neuronal form being slightly more diffuse (Figures 5, 6A). nsPIST localized to discrete areas of the Golgi.

FIGURE 5
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Figure 5. Single confocal slices of cells expressing CFP-tagged PIST isoforms (blue). The Golgi apparatus was stained with BODIPY-C5 ceramide (red). Scale bar, 10 μm.

FIGURE 6
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Figure 6. Cellular distribution of KV10.1_Venus and CFP-tagged PIST. CFP-PIST, when expressed alone (A), reproduced from Figure 5, gave a diffuse distribution with preferential location to the Golgi (stained in red). Overexpressed KV10.1 showed a preferentially intracellular distribution (B). When co-expressed (C), both CFP-PIST and KV10.1 were detectable at the cell periphery, compatible with an increase of membrane localization of both proteins. (D) represents a larger magnification of the area represented by a white square in the “merged” panel in (C), Scale bar: 10 μm.

Co-expression of a Venus-tagged KV10.1 led to a slight change in the localization of PIST only, a fraction of which showed expression at the membrane; this also resulted in a clearer membrane localization of KV10.1 itself. Expressed alone, KV10.1_Venus showed a typical distribution of this ion channel in the cell, where most KV10.1 stays intracellular and only weak expression can be seen at the membrane (Figure 6B). PIST coexpression led to peripheral venus signal compatible with increased channel localization at the plasma membrane (Figures 6C,D). Overexpression of nPIST as well as its short form nsPIST did not change KV10.1 localization in the cell (not shown). This data further supports the idea that a trafficking effect of PIST might be involved in the regulation of macroscopic current amplitude.

PIST Changes Membrane Expression of KV10.1

To get more quantitative data we made use of another system recently established in our lab, which is based on KV10.1 bearing an α-bungarotoxin (BTX) binding site between transmembrane segments 3 and 4 (KV10.1_BBS). Different labeling techniques with an α-BTX-biotin conjugate allow to distinguish between whole cell, membrane bound and internalized KV10.1 out of stably expressing cells and to quantify this different fractions via ELISA (Kohl et al., 2011). Specific labeling of membrane KV10.1_BBS is possible by incubating intact cells stably expressing KV10.1_BBS at low temperature (to avoid internalization) with an α-BTX-biotin conjugate. Quantification of this fraction of membrane bound KV10.1_BBS revealed that the overexpression of CFP-tagged PIST can indeed increase surface KV10.1_BBS to 214 ± 28% of control levels (Figure 7). In agreement with findings in the oocyte expression system, the neuronal forms of PIST (nPIST and nsPIST) did not have any effect on membrane KV10.1_BBS, as values were not different (94 ± 15% nPIST, 91 ± 16% nsPIST) from control levels.

FIGURE 7
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Figure 7. Over-expression of PIST isoforms differently influences cellular fractions of KV10.1_BBS. Different labeling protocols allowed distinguishing between whole cell, membrane bound and internalized KV10.1_BBS by ELISA (see Materials and Methods). The fraction of membrane bound KV10.1_BBS, labeled on living cells, was clearly enriched by expression of PIST but not of the other isoforms (N = 3; ***p < 0.001).

Both increased expression levels and increased stability of plasma membrane KV10.1_BBS could explain this observation. To distinguish between both possibilities, we examined the effect of overexpressing PIST isoforms on whole cell KV10.1_BBS, labeled after lysis of the cells. Neither isoform of PIST had an effect on the amount of total KV10.1_BBS (Figure 7), giving values of 96 ± 5% (PIST), 92 ± 3% (nsPIST) or 102 ± 9% (nPIST) in comparison to control levels, indicating that neither isoform of PIST affect total expression levels of KV10.1_BBS. Therefore, they may be involved in the transport of this voltage gated ion channel to and from the plasma membrane.

The KV10.1_BBS system easily allows for the quantification of internalized KV10.1_BBS within a given time frame (Kohl et al., 2011). For this, we incubated living cells with α-BTX-biotin conjugate at 37°C and allowed internalization for one hour. Removal of the label of membrane-bound KV10.1_BBS by acid wash afterwards leaves only the internalized labeled KV10.1_BBS for quantification. Interestingly, neither isoform had an effect on the total amount of internalized KV10.1_BBS (PIST 90 ± 9%, nsPIST 92 ± 13%, nPIST 98 ± 8%). As membrane expression of KV10.1_BBS is clearly increased in response to PIST overexpression (Figure 7), one would expect more channels internalized if the rate of internalization is not affected by this protein.

Discussion

In this study we identified PIST as a binding partner of KV10.1 and cloned two new isoforms of PIST. The novel variants lack a significant fraction of the PDZ domain and are expressed in all tissues examined, although with lower abundance than their respective longer forms. The shorter form was also detected at the protein level in HEK293 cells (Figure 3). The new isoforms correspond to skipping of exon 6 in the human gene, encoding for most of the PDZ domain. Interaction of KV10.1 and PIST appears to require the PDZ domain of the latter, since deletion of this domain reduced binding in pull-down assays. On the channel side, the C-terminus of the protein was the part that showed interaction with PIST in a yeast two-hybrid screen, and therefore is a probable mediator of the interaction. However, the interaction might need an additional binding partners, because pull down experiments using recombinant purified proteins (KV10.1 C-terminus and PIST) were unsuccessful (not shown).

We found KV10.1-mediated current to be influenced by the overexpression of PIST in Xenopus oocytes. This effect is apparently not due to changes in the electrophysiological properties of KV10.1 but rather due to a change in the surface expression of the channel, which was clearly enhanced by PIST as indicated by surface-labeling experiments. Binding of endogenous PIST was enhanced by overexpression of CFP-tagged PIST, leading to the idea that binding gives a positive feedback that stabilizes or improves association of both proteins in vivo.

Forced expression of the short isoform of PIST (sPIST) acts as a dominant negative in Xenopus oocytes, in the sense that leads to reduction of functional expression of KV10.1 (Figure 4D). These data are in good agreement with previous observations regarding the interaction of PIST with AMPA receptors (Cuadra et al., 2004). Although binding of several proteins to the coiled-coil region of PIST is reported, functional consequences have only been described through interaction with the PDZ domain (Cuadra et al., 2004; He et al., 2004; Cheng et al., 2005; Wente et al., 2005a; Xu et al., 2010). Deletion of the PDZ domain results in a dominant negative effect in AMPA receptor clustering (Cuadra et al., 2004). This led to the hypothesis that the short forms of PIST might serve as negative regulators of PIST function, in good agreement with our observations in oocytes.

nPIST, which differs from PIST by an insertion of eight amino acids, was not able to regulate KV10.1 in our model systems. Since the insertion occurs in the coiled-coil region, the lack of regulation by nPIST indicates that, although the physical interaction with KV10.1 depends on the PDZ domain, the functional effect requires additionally the coiled-coil region of PIST. This raises the question if and how the surface expression of KV10.1, which is normally expressed exclusively in the central nervous system (Pardo et al., 1999), is controlled by nPIST and PIST in vivo. We cannot exclude from our data that nPIST might also control surface levels of KV10.1 in vivo because, as already mentioned, it is likely that additional partners are required the functional interaction of nPIST and KV10.1. These additional factor(s) could be different for nPIST and PIST, and its (their) absence might preclude an nPIST-mediated effect in our systems. Our results point to an at least much more efficient influence of PIST compared to nPIST in positively affecting KV10.1 surface expression. This implies that, in tumors outside the brain, the surface expression of ectopically expressed KV10.1 would be additionally boosted by the expression of PIST.

From our data, we can only speculate about the mechanism by which PIST can influence the surface expression of KV10.1. However, we can exclude mechanisms involving an overall increased amount of KV10.1, induced either by enhanced synthesis or decreased degradation of the ion channel, since we observed no changes in total KV10.1 in cells overexpressing PIST.

Although there are apparently more active channels at the membrane, the amount of internalized KV10.1 is not altered by overexpression of PIST, indicating that PIST induces a more efficient transport of KV10.1 to the plasma membrane. However, this interpretation might be an oversimplification, because it would require that the amount of KV10.1 channels at the plasma membrane does not influence the rate of internalization, which is counterintuitive. Trafficking of KV10.1 is not yet completely understood. We have recently described Rabaptin 5 (RABEP1) as an interaction partner of KV10.1, linking this ion channel to early endosomes (Ninkovic et al., 2012). As an early event in the maturation and function of early endosomes Rabaptin 5 is able to bind and stabilize the GTP form Rab5, which then recruits effector proteins (Stenmark et al., 1995; Lippe et al., 2001). PIST interacts with Syntaxin 6, a member of the Syntaxin family of SNAREs that is also a Rab5 effector (Charest et al., 2001). Syntaxin 6, together with Syntaxin 13, VTI1a (Vps10p tail interactor 1) and VAMP4 were identified as the SNARE machinery involved in homotypic fusion of early endosomes to where it is recruited by direct binding of early endosomal antigen-1 (Simonsen et al., 1999; Brandhorst et al., 2006; Zwilling et al., 2007). PIST could therefore interfere with this machinery to block KV10.1 internalization. Inhibition of internalization processes by overexpression of a dominant-negative point mutant of Rab5(S34N), shown to block endocytosis (Li and Stahl, 1993), drastically reduced the amount of internalized KV10.1, but was not able to increase its surface expression significantly (data not shown). If PIST only impairs endocytosis of KV10.1, we therefore would not expect such a high increase in its surface expression as seen here.

PIST is also involved in sorting mechanisms, facilitating the trafficking of CFTR to lysosomes when over-expressed (Cheng et al., 2002, 2004, 2005; Gentzsch et al., 2003). Moreover, the Rho GTPase TC10 can bind to PIST and modulate this effect (Neudauer et al., 2001; Cheng et al., 2005). Another example of PIST function is the enhancement of synaptic clustering of AMPA receptors in hippocampal neurons in response to overexpression of the neuronal form of PIST (Cuadra et al., 2004). Deletion of the PDZ domain served as dominant negative form of nPIST clearly reducing AMPA clustering at the membrane (Cuadra et al., 2004). Endosomal sorting of AMPA receptors beginning in early endosomes towards either recycling or late endosomes is well documented to be a main process in long term synaptic plasticity in hippocampal neurons [reviewed in Hanley (2010)]. Therefore nPIST, like PIST for CFTR, might be involved in sorting processes for AMPA receptors, favoring recycling.

A similar effect might be postulated for the KV10.1-PIST interaction. PIST may serve as a molecular switch to control the fate of the channel after internalization in early endosomes by enhancing its recycling. This would lead to an increased membrane expression of the channel, while the amount of internalized KV10.1 would be reduced, leading in our case to equal amounts of internalized KV10.1 despite drastic changes in the membrane expression. Another interaction partner of PIST might regulate this process by binding to the coiled-coil region of PIST, like TC10 does for CFTR (Cheng et al., 2005). Candidate proteins for this interaction are small Rho GTPases like TC10 or Rab proteins, as Rab6a was shown to bind PIST (Bergbrede et al., 2009). The insertion of eight amino acids in the neuronal form of PIST lies in the region putatively responsible for this interaction, leading to the hypothesis that the isoforms can be regulated by different binding partners. These binding partners might decide if a protein bound to the PDZ domain of PIST is sorted to lysosomes or to a recycling process. The lack of the right binding partner for nPIST in our systems would explain why we observed a different behavior of PIST and nPIST although both are able to bind KV10.1 in pull down assays. The shorter forms of PIST may serve as a dominant negative variant, whose expression controls PIST function in vivo by trapping the binding partners of the coiled-coil region without linking them to effected proteins like KV10.1.

Conflict of Interest Statement

Luis A. Pardo is shareholder at iOnGen AG. The other authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Acknowledgments

We thank Prof. Nils Brose for the gift of the cDNA library used in the yeast two-hybrid screen, and Dr. Roser Ufartes for sharing mouse cDNAs. We also thank Sabine Klöppner, Tanja Nilsson and Annett Sporning for excellent technical support.

References

Agarwal, J. R., Griesinger, F., Stuhmer, W., and Pardo, L. A. (2010). The potassium channel Ether a go-go is a novel prognostic factor with functional relevance in acute myeloid leukemia. Mol. Cancer 9, 18.

Pubmed Abstract | Pubmed Full Text

Asher, V., Khan, R., Warren, A., Shaw, R., Schalkwyk, G. V., Bali, A., et al. (2010). The Eag potassium channel as a new prognostic marker in ovarian cancer. Diagn. Pathol. 5:78. doi: 10.1186/1746-1596-5-78

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Bergbrede, T., Chuky, N., Schoebel, S., Blankenfeldt, W., Geyer, M., Fuchs, E., et al. (2009). Biophysical analysis of the interaction of Rab6a GTPase with its effector domains. J. Biol. Chem. 284, 2628–2635. doi: 10.1074/jbc.M806003200

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Birch, A. H., Arcand, S. L., Oros, K. K., Rahimi, K., Watters, A. K., Provencher, D., et al. (2011). Chromosome 3 anomalies investigated by genome wide SNP analysis of benign, low malignant potential and low grade ovarian serous tumours. PLoS ONE 6:e28250. doi: 10.1371/journal.pone.0028250

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Brandhorst, D., Zwilling, D., Rizzoli, S. O., Lippert, U., Lang, T., and Jahn, R. (2006). Homotypic fusion of early endosomes: SNAREs do not determine fusion specificity. Proc. Natl. Acad. Sci. U.S.A. 103, 2701–2706. doi: 10.1073/pnas.0511138103

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Charest, A., Lane, K., McMahon, K., and Housman, D. E. (2001). Association of a novel PDZ domain-containing peripheral Golgi protein with the Q-SNARE (Q-soluble N-ethylmaleimide-sensitive fusion protein (NSF) attachment protein receptor) protein syntaxin 6. J. Biol. Chem. 276, 29456–29465. doi: 10.1074/jbc.M104137200

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Charest, A., Lane, K., McMahon, K., Park, J., Preisinger, E., Conroy, H., et al. (2003). Fusion of FIG to the receptor tyrosine kinase ROS in a glioblastoma with an interstitial del(6) (q21q21). Genes Chromosomes Cancer 37, 58–71. doi: 10.1002/gcc.10207

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Chen, Y., Sanchez, A., Rubio, M. E., Kohl, T., Pardo, L. A., and Stuhmer, W. (2011). Functional K(v)10.1 channels localize to the inner nuclear membrane. PLoS ONE 6:e19257. doi: 10.1371/journal.pone.0019257

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Cheng, J., Moyer, B. D., Milewski, M., Loffing, J., Ikeda, M., Mickle, J. E., et al. (2002). A Golgi-associated PDZ domain protein modulates cystic fibrosis transmembrane regulator plasma membrane expression. J. Biol. Chem. 277, 3520–3529. doi: 10.1074/jbc.M110177200

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Cheng, J., Wang, H., and Guggino, W. B. (2004). Modulation of mature cystic fibrosis transmembrane regulator protein by the PDZ domain protein CAL. J. Biol. Chem. 279, 1892–1898. doi: 10.1074/jbc.M308640200

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Cheng, J., Wang, H., and Guggino, W. B. (2005). Regulation of cystic fibrosis transmembrane regulator trafficking and protein expression by a Rho family small GTPase TC10. J. Biol. Chem. 280, 3731–3739. doi: 10.1074/jbc.M410026200

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Cuadra, A. E., Kuo, S. H., Kawasaki, Y., Bredt, D. S., and Chetkovich, D. M. (2004). AMPA receptor synaptic targeting regulated by stargazin interactions with the Golgi-resident PDZ protein nPIST. J. Neurosci. 24, 7491–7502. doi: 10.1523/JNEUROSCI.1255-04.2004

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Ding, X. W., Luo, H. S., Jin, X., Yan, J. J., and Ai, Y. W. (2007a). Aberrant expression of Eag1 potassium channels in gastric cancer patients and cell lines. Med. Oncol. 24, 345–350.

Pubmed Abstract | Pubmed Full Text

Ding, X. W., Yan, J. J., An, P., Lu, P., and Luo, H. S. (2007b). Aberrant expression of ether a go-go potassium channel in colorectal cancer patients and cell lines. World J. Gastroenterol. 13, 1257–1261.

Pubmed Abstract | Pubmed Full Text

Ding, X. W., Wang, X. G., Luo, H. S., Tan, S. Y., Gao, S., Luo, B., et al. (2008). Expression and prognostic roles of Eag1 in resected esophageal squamous cell carcinomas. Dig. Dis. Sci. 53, 2039–2044.

Pubmed Abstract | Pubmed Full Text

Downie, B. R., Sanchez, A., Knotgen, H., Contreras-Jurado, C., Gymnopoulos, M., Weber, C., et al. (2008). Eag1 expression interferes with hypoxia homeostasis and induces angiogenesis in tumors. J. Biol. Chem. 283, 36234–36240. doi: 10.1074/jbc.M801830200

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Gentzsch, M., Cui, L., Mengos, A., Chang, X. B., Chen, J. H., and Riordan, J. R. (2003). The PDZ-binding chloride channel ClC-3B localizes to the Golgi and associates with cystic fibrosis transmembrane conductance regulator-interacting PDZ proteins. J. Biol. Chem. 278, 6440–6449. doi: 10.1074/jbc.M211050200

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Gomez-Varela, D., Zwick-Wallasch, E., Knotgen, H., Sanchez, A., Hettmann, T., Ossipov, D., et al. (2007). Monoclonal antibody blockade of the human Eag1 potassium channel function exerts antitumor activity. Cancer Res. 67, 7343–7349. doi: 10.1158/0008-5472.CAN-07-0107

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Gu, T.-L., Deng, X., Huang, F., Tucker, M., Crosby, K., Rimkunas, V., et al. (2011). Survey of tyrosine kinase signaling reveals ROS kinase fusions in human cholangiocarcinoma. PLoS ONE 6:e15640. doi: 10.1371/journal.pone.0015640

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Hanley, J. G. (2010). Endosomal sorting of AMPA receptors in hippocampal neurons. Biochem. Soc. Trans. 38, 460–465. doi: 10.1042/BST0380460

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

He, J., Bellini, M., Xu, J., Castleberry, A. M., and Hall, R. A. (2004). Interaction with cystic fibrosis transmembrane conductance regulator-associated ligand (CAL) inhibits beta1-adrenergic receptor surface expression. J. Biol. Chem. 279, 50190–50196. doi: 10.1074/jbc.M404876200

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Hemmerlein, B., Weseloh, R. M., De Queiroz, F. M., Knötgen, H., Sánchez, A., Rubio, M. E., et al. (2006). Overexpression of Eag1 potassium channels in clinical tumours. Mol. Cancer 5, 41.

Pubmed Abstract | Pubmed Full Text

Herrmann, S., Ninkovic, M., Kohl, T., Lorinczi, E., and Pardo, L. A. (2012). Cortactin controls surface expression of the voltage-gated potassium channel KV10.1. J. Biol. Chem. 287, 44151–44163. doi: 10.1074/jbc.M112.372540

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Hicks, S. W., and Machamer, C. E. (2005). Isoform-specific interaction of golgin-160 with the Golgi-associated protein PIST. J. Biol. Chem. 280, 28944–28951. doi: 10.1074/jbc.M504937200

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Kohl, T., Lorinczi, E., Pardo, L. A., and Stuhmer, W. (2011). Rapid internalization of the oncogenic K+ channel K(V)10.1. PLoS ONE 6:e26329. doi: 10.1371/journal.pone.0026329

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Li, G., and Stahl, P. D. (1993). Structure-function relationship of the small GTPase rab5. J. Biol. Chem. 268, 24475–24480.

Pubmed Abstract | Pubmed Full Text

Lippe, R., Miaczynska, M., Rybin, V., Runge, A., and Zerial, M. (2001). Functional synergy between Rab5 effector Rabaptin-5 and exchange factor Rabex-5 when physically associated in a complex. Mol. Biol. Cell 12, 2219–2228. doi: 10.1091/mbc.12.7.2219

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Mello De Queiroz, F., Suarez-Kurtz, G., Stuhmer, W., and Pardo, L. A. (2006). Ether a go-go potassium channel expression in soft tissue sarcoma patients. Mol. Cancer 5, 42.

Pubmed Abstract | Pubmed Full Text

Neudauer, C. L., Joberty, G., and Macara, I. G. (2001). PIST: a novel PDZ/coiled-coil domain binding partner for the rho-family GTPase TC10. Biochem. Biophys. Res. Commun. 280, 541–547. doi: 10.1006/bbrc.2000.4160

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Ninkovic, M., Mitkovski, M., Kohl, T., Stuhmer, W., and Pardo, L. A. (2012). Physical and functional interaction of K(V)10.1 with Rabaptin-5 impacts ion channel trafficking. FEBS Lett. 586, 3077–3084. doi: 10.1016/j.febslet.2012.07.055

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Nishiyama, J., Matsuda, K., Kakegawa, W., Yamada, N., Motohashi, J., Mizushima, N., et al. (2010). Reevaluation of neurodegeneration in lurcher mice: constitutive ion fluxes cause cell death with, not by, autophagy. J. Neurosci. 30, 2177–2187. doi: 10.1523/JNEUROSCI.6030-09.2010

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Occhiodoro, T., Bernheim, L., Liu, J. H., Bijlenga, P., Sinnreich, M., Bader, C. R., et al. (1998). Cloning of a human ether-a-go-go potassium channel expressed in myoblasts at the onset of fusion. FEBS Lett. 434, 177–182. doi: 10.1016/S0014-5793(98)00973-9

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Okamoto, M., and Sudhof, T. C. (1997). Mints, Munc18-interacting proteins in synaptic vesicle exocytosis. J. Biol. Chem. 272, 31459–31464. doi: 10.1074/jbc.272.50.31459

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Ousingsawat, J., Spitzner, M., Puntheeranurak, S., Terracciano, L., Tornillo, L., Bubendorf, L., et al. (2007). Expression of voltage-gated potassium channels in human and mouse colonic carcinoma. Clin. Cancer Res. 13, 824–831. doi: 10.1158/1078-0432.CCR-06-1940

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Pardo, L. A., Del Camino, D., Sanchez, A., Alves, F., Bruggemann, A., Beckh, S., et al. (1999). Oncogenic potential of EAG K(+) channels. EMBO J. 18, 5540–5547. doi: 10.1093/emboj/18.20.5540

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Schindelin, J., Arganda-Carreras, I., Frise, E., Kaynig, V., Longair, M., Pietzsch, T., et al. (2012). Fiji: an open-source platform for biological-image analysis. Nat. Methods 9, 676–682. doi: 10.1038/nmeth.2019

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Shin, Y. C., Seo, E. K., Jeon, J. H., and Park, H. H. (2013). Crystallization and preliminary X-ray crystallographic studies of the coiled-coil domain of PIST. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 69, 468–471. doi: 10.1107/S1744309113007082

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Simonsen, A., Gaullier, J. M., D'Arrigo, A., and Stenmark, H. (1999). The Rab5 effector EEA1 interacts directly with syntaxin-6. J. Biol. Chem. 274, 28857–28860. doi: 10.1074/jbc.274.41.28857

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Stenmark, H., Vitale, G., Ullrich, O., and Zerial, M. (1995). Rabaptin-5 is a direct effector of the small GTPase Rab5 in endocytic membrane fusion. Cell 83, 423–432. doi: 10.1016/0092-8674(95)90120-5

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Stuhmer, W. (1998). Electrophysiologic recordings from Xenopus oocytes. Meth. Enzymol. 293, 280–300. doi: 10.1016/S0076-6879(98)93019-1

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Suehara, Y., Arcila, M., Wang, L., Hasanovic, A., Ang, D., Ito, T., et al. (2012). Identification of KIF5B-RET and GOPC-ROS1 fusions in lung adenocarcinomas through a comprehensive mRNA-based screen for tyrosine kinase fusions. Clin. Cancer Res. 18, 6599–6608. doi: 10.1158/1078-0432.CCR-12-0838

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Ufartes, R., Schneider, T., Mortensen, L. S., De Juan Romero, C., Hentrich, K., Knoetgen, H., et al. (2013). Behavioural and functional characterization of Kv10.1 (Eag1) knockout mice. Hum. Mol. Genet. 22, 2247–2262.

Pubmed Abstract | Pubmed Full Text

Vojtek, A. B., Hollenberg, S. M., and Cooper, J. A. (1993). Mammalian Ras interacts directly with the serine/threonine kinase Raf. Cell 74, 205–214. doi: 10.1016/0092-8674(93)90307-C

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Wadhwa, S., Wadhwa, P., Dinda, A. K., and Gupta, N. P. (2009). Differential expression of potassium ion channels in human renal cell carcinoma. Int. Urol. Nephrol. 41, 251–257. doi: 10.1007/s11255-008-9459-z

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Warmke, J. W., and Ganetzki, B. (1994). A family of potassium channel genes related to eag in Drosophila and mammals. Proc. Natl. Acad. Sci. U.S.A. 91, 3438–3442. doi: 10.1073/pnas.91.8.3438

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Wente, W., Efanov, A. M., Treinies, I., Zitzer, H., Gromada, J., Richter, D., et al. (2005a). The PDZ/coiled-coil domain containing protein PIST modulates insulin secretion in MIN6 insulinoma cells by interacting with somatostatin receptor subtype 5. FEBS Lett. 579, 6305–6310.

Pubmed Abstract | Pubmed Full Text

Wente, W., Stroh, T., Beaudet, A., Richter, D., and Kreienkamp, H. J. (2005b). Interactions with PDZ domain proteins PIST/GOPC and PDZK1 regulate intracellular sorting of the somatostatin receptor subtype 5. J. Biol. Chem. 280, 32419–32425.

Pubmed Abstract | Pubmed Full Text

Xu, Z., Oshima, K., and Heller, S. (2010). PIST regulates the intracellular trafficking and plasma membrane expression of cadherin 23. BMC Cell Biol. 11:80. doi: 10.1186/1471-2121-11-80

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Yao, R., Maeda, T., Takada, S., and Noda, T. (2001). Identification of a PDZ domain containing Golgi protein, GOPC, as an interaction partner of frizzled. Biochem. Biophys. Res. Commun. 286, 771–778. doi: 10.1006/bbrc.2001.5430

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Yue, Z., Horton, A., Bravin, M., Dejager, P. L., Selimi, F., and Heintz, N. (2002). A novel protein complex linking the delta 2 glutamate receptor and autophagy: implications for neurodegeneration in lurcher mice. Neuron 35, 921–933. doi: 10.1016/S0896-6273(02)00861-9

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Zhang, J., Cheng, S., Xiong, Y., Ma, Y., Luo, D., Jeromin, A., et al. (2008). A novel association of mGluR1a with the PDZ scaffold protein CAL modulates receptor activity. FEBS Lett. 582, 4117–4124. doi: 10.1016/j.febslet.2008.10.054

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Zwilling, D., Cypionka, A., Pohl, W. H., Fasshauer, D., Walla, P. J., Wahl, M. C., et al. (2007). Early endosomal SNAREs form a structurally conserved SNARE complex and fuse liposomes with multiple topologies. EMBO J. 26, 9–18. doi: 10.1038/sj.emboj.7601467

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Keywords: potassium channels, KV10.1, KCNH1, PIST, FIG, GOPC, trafficking

Citation: Herrmann S, Ninkovic M, Kohl T and Pardo LA (2013) PIST (GOPC) modulates the oncogenic voltage-gated potassium channel KV10.1. Front. Physiol. 4:201. doi: 10.3389/fphys.2013.00201

Received: 14 May 2013; Accepted: 17 July 2013;
Published online: 14 August 2013.

Edited by:

Andrea Becchetti, University of Milano-Bicocca, Italy

Reviewed by:

Ita O'Kelly, University of Southampton, UK
Roland Schönherr, Jena University Hospital, Germany

Copyright © 2013 Herrmann, Ninkovic, Kohl and Pardo. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

*Correspondence: Luis A. Pardo, AG Oncophysiology and Department of Molecular Biology of Neuronal Signals, Max-Planck Institute of Experimental Medicine, Hermann-Rein-Str. 3, 37075 Göttingen, Germany e-mail: pardo@em.mpg.de

Present address: Milena Ninkovic, Department of Neurosurgery, Georg-August University Medical Center, Göttingen, Germany;
Tobias Kohl, Department of Cardiology and Pneumology, Translational Cardiology, Georg-August University Medical Center, Göttingen, Germany