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Perspective ARTICLE

Front. Physiol., 21 January 2014 | https://doi.org/10.3389/fphys.2014.00007

Genetic evidence for conserved non-coding element function across species–the ears have it

Eric E. Turner1,2,3* and Timothy C. Cox2,4,5,6
  • 1Center for Integrative Brain Research, Seattle Children's Research Institute, Seattle, WA, USA
  • 2Center on Human Development and Disability, University of Washington, Seattle, WA, USA
  • 3Department of Psychiatry and Behavioral Sciences, University of Washington, Seattle, WA, USA
  • 4Department of Pediatrics (Craniofacial Medicine), University of Washington, Seattle, WA, USA
  • 5Department of Anatomy and Developmental Biology, Monash University, Clayton, VIC, Australia
  • 6Center for Developmental Biology and Regenerative Medicine, Seattle Children's Research Institute, Seattle, WA, USA

Comparison of genomic sequences from diverse vertebrate species has revealed numerous highly conserved regions that do not appear to encode proteins or functional RNAs. Often these “conserved non-coding elements,” or CNEs, can direct gene expression to specific tissues in transgenic models, demonstrating they have regulatory function. CNEs are frequently found near “developmental” genes, particularly transcription factors, implying that these elements have essential regulatory roles in development. However, actual examples demonstrating CNE regulatory functions across species have been few, and recent loss-of-function studies of several CNEs in mice have shown relatively minor effects. In this Perspectives article, we discuss new findings in “fancy” rats and Highland cattle demonstrating that function of a CNE near the Hmx1 gene is crucial for normal external ear development and when disrupted can mimic loss-of function Hmx1 coding mutations in mice and humans. These findings provide important support for conserved developmental roles of CNEs in divergent species, and reinforce the concept that CNEs should be examined systematically in the ongoing search for genetic causes of human developmental disorders in the era of genome-scale sequencing.

The Concept of Conserved Non-Coding Elements

In the past decade the availability of the genomic sequences of diverse animal species has led to the recognition of a high degree of sequence conservation outside protein coding regions, particularly across vertebrate genomes. While some of this sequence conservation may be accounted for by non-coding functional RNA, most of the conserved regions are believed to have cis-regulatory function. Genomic regions that can be recognized by the alignment of distantly related vertebrate species, such as fish, rodents, and humans, have been termed “conserved non-coding elements” or CNEs (Nelson and Wardle, 2013). The concept of a CNE is related to the term “ultraconserved element” (UCE), although this latter designation refers specifically to sequences of at least 200 bp with 100% conservation between rodent and human genomes (Sandelin et al., 2004), which is not an essential determinant of function (Visel et al., 2008). CNEs are also conceptually related to “cis-regulatory modules,” or CRMs, which are defined as regulatory elements that interact with transcription factors to determine tissue-specific gene expression (Howard and Davidson, 2004). However, the recognizable conservation of an extended sequence across species is not a defining feature of CRMs.

Indirect evidence for the regulatory function of CNEs is supplied by the frequent occurrence of these sequences near developmental regulatory genes, particularly transcription factors (Sandelin et al., 2004). This observation reinforces the impression that CNEs regulate core programs of gene expression related to cell identity and morphogenesis which must be solved by all vertebrates. More direct evidence for enhancer function has been obtained from experiments in which CNEs have been used to drive tissue-specific gene expression in zebrafish (Woolfe et al., 2005) and in mice (Pennacchio et al., 2006). Similarly, two reports to date describing efforts to delete numerous CNEs with known enhancer function in mice have shown surprisingly minor phenotypic effects (Ahituv et al., 2007; Attanasio et al., 2013). This low yield in targeted or “reverse” genetic experiments demonstrates that forward genetic strategies have an important role in determining CNE function.

CNEs as Sites of Disease Causing Mutations: Promises Unfulfilled

If CNEs have conserved regulatory functions, mutations in these sequences should present with related phenotypes in diverse species. However, support for this prediction to date has been sparse. One of the few clear examples of a conserved CNE phenotype relates to a long-range enhancer regulating expression of the morphogen Shh in the developing limb, which is the site of causative alleles in preaxial polydactyly in humans (Lettice and Hill, 2005), and distal limb defects in the mouse insertional mutant sasquatch (ssq, Sharpe et al., 1999). The function of the relevant enhancer, residing ~1Mb from the coding gene, has been confirmed by targeted mutagenesis (Sagai et al., 2005). The causative alleles for a small number of other human genetic disorders have mapped outside coding sequence (Amiel et al., 2010), but in most cases the gene regulatory mechanisms involved are unclear, and cross-species demonstration of CNE function has not been obtained. This is partly because the assignment of function to sequence variation found in CNEs is more difficult than that found in coding sequence, since the “transcriptional genetic code” that governs transcription factor binding to enhancer sequences is less well-understood and more complex than the translational genetic code governing coding sequence.

Hmx1: Taking The Bull by the Horns (or Ears)

Two recent reports of mutations affecting development of the external ear, one in “fancy” rats kept by amateur breeders in the United States, and the other in Highland cattle bred in Switzerland, provide exciting new evidence for disruption of CNE function as a mechanism underlying developmental disorders (Quina et al., 2012a; Koch et al., 2013). The rat “dumbo” (dmbo) mutation is a recessive trait causing ventral displacement and rotation of the external ear (pinna), leading to protruding ears akin to the cartoon elephant that gives the strain its name (Figure 1, Kuramoto et al., 2010). The origin of the dumbo phenotype is obscure, but it was probably first identified by hobbyist breeders in the western United States, and it is considered a desirable trait in fancy rats. The “crop ear” trait in Highland cattle shows partially dominant inheritance of a moderately to severely truncated (or cropped) ear deformity, which may vary according to gene dosage and genetic background (Scheider et al., 1994). Crop-eared Highland cattle are found in herds in Europe, North America, and Australia. In some regions the breeding of animals with severe crop ear defects is discouraged, in others it is ignored, since it has no other known effect on the health of the affected animal.

FIGURE 1
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Figure 1. Rats exhibiting normal (top) and Dumbo (bottom) ear phenotypes Kuramoto et al. (2010).

Genetic mapping of the rat dmbo and bovine crop ear alleles converged on chromosomal locations that encompass the Hmx1 gene, a homeodomain transcription factor (Figure 2A). Coding mutations at the Hmx1 locus are known to account for two mouse ear variants, one also called “dumbo,” (dmbo), and one called “misplaced ears” (mpe, Munroe et al., 2009). The mouse dmbo and mpe alleles consist of a nonsense mutation and an 8 bp coding region deletion, respectively, and are likely to be null alleles. In humans, a coding variant of the HMX1 gene causes a recessive disorder called oculoauricular syndrome (OAS), characterized by deformities of the pinna and also variable eye defects (Schorderet et al., 2008; Vaclavik et al., 2011). The human HMX1 allele associated with OAS consists of a 26 bp deletion in the coding region, resulting in a frameshift, which is also likely to be a null allele.

FIGURE 2
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Figure 2. Genomic location of mutations affecting pinna development at the Hmx1 locus. (A) The chromosomal relationship between the Hmx1 gene (and in some species not shown, two related Hmx genes), a distal Hmx1 CNE, and the neighboring gene encoding carboxypeptidase Z (Cpz) is conserved in all known vertebrate genomes (Quina et al., 2012b). The human OAS allele is a 26 bp deletion in the coding region of Hmx1, resulting in a frameshift, which is likely to be a null allele (Schorderet et al., 2008). The mouse dmbo and mpe alleles also map to the Hmx1 coding region, and are also likely to be null alleles (Jiang et al., 2002; Munroe et al., 2009). In contrast, the rat dmbo allele is a 5777 bp deletion encompassing the CNE (Chieffo et al., 1997), and the bovine crop ear (CE) allele is a 76 bp duplication within the CNE (Koch et al., 2013). (B) Alignment of the Hmx1 distal CNE sequence from the human, mouse, rat, xenopus tropicalis and cow genomes. Conserved bases are shown in upper case. Alignment of the x. tropicalis sequence with the mammalian genomes identifies a core “ultraconserved” sequence (shaded area) that can be identified in all vertebrate genomes for which data are available (Quina et al., 2012b). The 76 bp CE allele consists of a duplication within this core element. The genomic coordinates of the sequences shown are as follows: human, GRCh37/hg19 chr4:8,702,169-8,702,467; mouse, GRCm38/mm10, chr5:35,466,174-35,466,471; rat, Baylor 3.4/rn4, chr14:80,916,333-80,916,631; xenopus tropicalis, JGI 4.2/xenTro3, GL173219:238,403-238,694; cow, Baylor Btau_4.6.1/bosTau7, chr6:120,904,093-120,904,404. (C) Conserved transcription factor binding sites (top) and VISTA plot (bottom) of the Hmx1 CNE. Five TF binding sites for TF families of known developmental significance occur in this region; only those sites conserved across seven mammalian species are shown. In a DNA sequence of this length, the random expected occurrence of Ebox sites is two, of homeobox sites is two, and of the other sites is zero. VISTA plot shows percent homology between human and mouse genomes. The conserved transcription factor search was performed using Mulan: http://mulan.dcode.org/.

Remarkably, the rat dmbo and bovine crop ear mutations do not affect the Hmx1 coding region. Instead, resequencing of the chromosomal regions containing Hmx1 in these species revealed mutations far downstream from the Hmx1 transcription unit (Figure 2A). In the rat, the dmbo allele consists of a 5777 bp deletion residing ~80 Mb downstream of the Hmx1 transcription unit. A CNE of ~300 bp within this region exhibits very high identity (85–98%) between all mammalian species, and exhibits a core of conserved sequence that is retained in reptiles, fish and amphibians (Figure 2B). The bovine crop ear allele consists of a 76 bp duplication within the most highly conserved part of this CNE.

Supporting evidence for tissue-specific enhancer function within the rat dmbo deletion region, likely conferred by this CNE, has been provided by studies of developmental gene expression in dumbo rat embryos (Quina et al., 2012a). Embryonic dumbo rats exhibit loss of Hmx1 protein expression specifically in the craniofacial mesenchyme which contributes to the pinna. In contrast, expression is normal in the cranial sensory nervous system, where Hmx1 also has an important developmental role (Quina et al., 2012b). The Hmx1 CNE is rich in predicted binding sites for transcription factor classes known to be important in regulating developmental processes (Figure 2C). Unfortunately, the prediction of enhancer function from sequence data is not reliable enough to infer whether the additional sites contained in the crop ear duplication will increase enhancer function or disrupt it.

The ear phenotype of dumbo rats closely resembles that seen in the mouse loss-of-function alleles, with pinnae that are slightly dysmorphic and ventrally displaced and rotated, giving the appearance of larger “floppy” ears. The bovine crop ear phenotype in contrast is a marked pinna deficiency. These differences may reflect apposing effects of the mutations: i.e., loss of Hmx1 expression in rats and increased expression in cattle. With respect to this, it is also interesting to note that the bovine crop ear trait is partially dominant, whilst the rodent Hmx1 alleles, including the rat dmbo CNE deletion, are recessive. It will be interesting to see whether these distinct modes of transmission result from the specific effect of the crop ear duplication event on Hmx1 enhancer function, or interspecies differences in the effects Hmx1 gene dosage on pinna development. Regardless, these cross-species examples reinforce the role of CNEs in conserved developmental processes and the notion that they can be a cause of developmental disorders.

Future Directions

Extensive evidence for conserved CNE enhancer function probably exists in the gene pool of the diverse vertebrates for which we now have nearly complete genomic sequence. The similarity of the phenotypes seen in Hmx1 coding and CNE mutants in mice, rats, cattle and humans indicate that CNE mutant phenotypes will not necessarily be subtle or polygenic, but instead may show Mendelian inheritance with high penetrance, and may phenocopy at least some of the features associated with null mutations in the genes they regulate. Although work in this area has just begun, three general strategies can advance the identification of functional mutations in CNEs that underlie human disease.

First, functional variants in CNEs will escape detection using the current methods of genomic analysis, particularly whole exome sequencing and transcriptome sequencing, since CNEs reside in introns and intergenic sequences. The CNEs identified in the human genome should therefore be systematically added to whole exome analysis of human genetic disorders, and examined for copy number variation, in order to appreciate the full range of sequence diversity in these regions that may cause disease phenotypes. The recognition of functional changes in CNEs would be greatly facilitated by a complete catalog of the genetic diversity in these loci, in the human population as well as across species.

Second, even when sequence variants in CNEs are identified, our ability to decode changes in CNE function based on sequence variation is still quite limited, especially for single nucleotide polymorphisms. General evidence for the enhancer function of a CNE, although not its specific regulatory role, can be derived from the analysis of local chromatin states by chromatin immunoprecipitation of modified histones and DNA methylation analysis (Schones and Zhao, 2008). Such genome-wide analyses of the human and mouse chromatin landscapes in several cell types are now accessible in searchable databases (Ernst et al., 2011; Shen et al., 2012). However, the general assignment of regulatory importance to a CNE-containing region does not determine the significance of any subtle genetic variants found within it. In principle this determination should be based on decoding the transcription factor binding sites within a CNE, thus allowing the recognition of important regulatory mutations, even those resulting from single-nucleotide changes. However, the flexible and combinatorial nature of transcription factor binding, and the large number of factors encoded by vertebrate genomes, make this an intrinsically difficult problem (Meireles-Filho and Stark, 2009), but one which is becoming tractable with advances in the systematic analysis of transcription factor binding sites (Jolma et al., 2013).

Finally, the definitive functional test of a disease-associated CNE can only be performed by demonstrating gain or loss of regulatory function in an appropriate in vivo vertebrate model, such as transgenic mice (Pennacchio et al., 2006; Ahituv et al., 2007) or zebrafish (Fisher et al., 2006), or the electroporation of enhancer-linked reporters in chick embryos (Simoes-Costa and Bronner, 2013). These methods are laborious and of moderate throughput, and thus will usually be applied to candidate CNEs that have already met some of the other criteria for probable significance. However, when successful, enhancer analysis in model species can reveal not only evidence for a disease-causing mechanism, but also the fundamental relationship between gene regulation and species-specific traits (Wittkopp and Kalay, 2012). For instance, in one effort at cross-species functional CNE analysis, replacement of a limb-specific enhancer in the Prx1 gene of the mouse with the orthologous sequence from a species of bat was shown to result in forelimb elongation (Cretekos et al., 2008). The key to an efficient strategy for definitive proof of CNE function in model systems will be to leverage as much as possible the available clues derived from forward genetic analysis of spontaneously occurring variants, such as the Hmx1-linked ear malformation syndromes, and also the gene regulatory framework provided by less-specific but more comprehensive genomic and epigenomic analysis.

Conflict of Interest Statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Glossary

Conserved non-coding element (CNE). A genomic region defined by sequence conservation between species, without known or predicted protein coding function. Such sequences are hypothesized to have regulatory functions.

Cis-regulatory module (CRM). A genomic region defined by function that contains recognition sites for transcription factors and affects the regulation of a gene. CRMs may be CNEs but are not necessarily recognizable from sequence conservation alone.

Enhancer. A region of chromosomal DNA that can mediate the activation of the tissue-specific expression of a gene. Most identified enhancers reside within introns or in the intergenic space between a gene and its chromosomal neighbors. However, examples exist of enhancers located at megabase distances from their regulatory targets.

H6 family homeobox 1 (Hmx1, HMX1/NKX5.3). A homeodomain gene/protein with expression in the developing external ear, eye, and peripheral sensory ganglia.

Oculoauricular syndrome (OAS). A human congenital syndrome involving malformation of the eye and external ear, one form of which has been linked to HMX1.

Ultraconserved element (UCE). A CNE with perfect or near-perfect interspecies conservation.

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Keywords: Hmx1, pinna, ear development, craniofacial morphogenesis, enhancer, oculoauricular syndrome

Citation: Turner EE and Cox TC (2014) Genetic evidence for conserved non-coding element function across species–the ears have it. Front. Physiol. 5:7. doi: 10.3389/fphys.2014.00007

Received: 21 November 2013; Accepted: 05 January 2014;
Published online: 21 January 2014.

Edited by:

Anna Petryk, University of Minnesota, USA

Reviewed by:

Harald Osmundsen, University of Oslo, Norway
Ralf Kist, Newcastle University, UK
Andrea Streit, King's College London, UK

Copyright © 2014 Turner and Cox. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

*Correspondence: Eric E. Turner, Seattle Children's Research Institute, 1900 Ninth Avenue, M/S C9S-10, Seattle, WA 98101, USA e-mail: eric.turner@seattlechildrens.org