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Front. Physiol. | doi: 10.3389/fphys.2018.00104

Expression of proinflammatory cytokines is upregulated in the hypothalamic paraventricular nucleus of Dahl salt-sensitive hypertensive rats

  • 1Department of Kinesiology and Integrative Physiology, Michigan Technological University, United States
  • 2Institute for Nursing and Health Research, Henan University, China
  • 3Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Xi’an Jiaotong University Health Science Center, Xi'an Jiaotong University, China

Accumulating evidence indicates that inflammation is implicated in hypertension. However, the role of brain proinflammatory cytokines (PICs) in salt sensitive hypertension remains to be determined. Thus, the objective of this study was to test the hypothesis that high salt (HS) diet increases PICs expression in the paraventricular nucleus (PVN) and leads to PVN neuronal activation. Eight-week-old male Dahl salt sensitive (Dahl S) rats, and age and sex matched normal Sprague Dawley (SD) rats were divided into two groups and fed with either a HS (4% NaCl) or normal salt (NS, 0.4% NaCl) diet for 5 consecutive weeks. HS diet induced hypertension and significantly increased cerebrospinal fluid (CSF) sodium concentration ([Na+]) in Dahl S rats, but not in normal SD rats. In addition, HS diet intake triggered increases in mRNA levels and immunoreactivities of PVN PICs including TNF-α, IL-6, and IL-1β, as well as Fra1, a chronic marker of neuronal activation, in Dahl S rats, but not in SD rats. Next, we investigated whether this increase in the expression of PVN PICs and Fra1 was induced by increased CSF [Na+]. Adult male SD rats were intracerebroventricular (ICV) infused with 8 µl of either hypertonic salt (4 µmol NaCl), mannitol (8 µmol, as osmolarity control), or isotonic salt (0.9% NaCl as vehicle control). 3 hours following the ICV infusion, rats were euthanized and their PVN PICs expression was measured. The results showed that central administration of hypertonic saline in SD rats significantly increased the expression of PICs including TNF-α, IL-6 and IL-1β, as well as neuronal activation marker Fra1, compared to isotonic NaCl controls and osmolarity controls. Finally, we tested whether the increase in PICs expression occurred in neurons. Incubation of hypothalamic neurons with 10 mM NaCl in a culture medium for 6 hours elicited significant increases in TNF-α, IL-6 and Fra1 mRNA levels. These observations, coupled with the important role of PICs in modulating neuronal activity and stimulating vasopressin release, suggest that HS intake induces an inflammatory state in the PVN, which, may in turn, augments sympathetic nerve activity and vasopressin secretion, contributing to the development of salt sensitive hypertension.

Keywords: paraventricular nucleus, proinflammatory cytokines, high slat diet, Hypertension, cerebrospinal fluid.

Received: 12 Nov 2017; Accepted: 31 Jan 2018.

Edited by:

Yulong Li, University of Nebraska Medical Center, United States

Reviewed by:

Huiyin Tu, Zhengzhou University, China
Zhigang Shi, Oregon Health & Science University, United States
Chengwen Sun, North Dakota State University, United States  

Copyright: © 2018 Jiang, Chapp, Fan, Larson, Hahka, Huber, Yan, Chen and Shan. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Dr. Zhiying Shan, Michigan Technological University, Department of Kinesiology and Integrative Physiology, Houghton, 49931, Michigan, United States, zhiyings@mtu.edu