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Front. Physiol. | doi: 10.3389/fphys.2018.00133

Cloning and analysis of gene expression of two Toll receptors in freshwater pearl mussel Hyriopsis cumingii

 Ying Huang1, Min Jin2 and  Qian Ren1, 3*
  • 1Nanjing Normal University, China
  • 2State Key Laboratory Breeding Base of Marine Genetic Resource, Third Institute of Oceanography, China
  • 3Co-Innovation Center for Marine Bio-Industry Technology of Jiangsu Province, China

Toll receptors are involved in innate immunity of invertebrates. In this study, we identify and characterize two Toll genes (named HcToll4 and HcToll5) from triangle sail mussel Hyriopsis cumingii. HcToll4 has complete cDNA sequence of 3162 bp and encodes a protein of 909 amino acids. HcToll5 cDNA is 4501 bp in length and encodes a protein of 924 amino acids. Both deduced HcToll4 and HcToll5 protein contain signal peptide, extracellular leucine rich repeats, and intracellular Toll/interleukin-1 receptor domains. Quantitative real-time PCR analysis revealed that HcToll4 and HcToll5 were largely distributed in the hepatopancreas and could be detected in the gills and mantle. HcToll4 and HcToll5 expression could respond to Staphylococcus aureus, Vibrio parahaemolyticus, White spot syndrome virus or Poly I:C challenge. RNA interference by siRNA results showed that HcToll4 and HcToll5 were involved in the regulation of theromacin (HcThe) and whey acidic protein (HcWAP) expression. Based on these results, HcToll4 and HcToll5 might play pivotal function in H. cumingii innate immune response.

Keywords: Hyriopsis cumingii, Tolls, RNAi, innate immunity, Anti-bacterial peptides

Received: 02 Nov 2017; Accepted: 09 Feb 2018.

Edited by:

Francesca Carella, University of Naples Federico II, Italy

Reviewed by:

Tiziano Verri, University of Salento, Italy
Serena Aceto, University of Naples Federico II, Italy  

Copyright: © 2018 Huang, Jin and Ren. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Prof. Qian Ren, Nanjing Normal University, Nanjing, China, renqian0402@126.com