Impact Factor 4.134

The 2nd most cited  journal in Physiology

Mini Review ARTICLE

Front. Physiol., 14 June 2018 | https://doi.org/10.3389/fphys.2018.00736

Thick Filament Mechano-Sensing in Skeletal and Cardiac Muscles: A Common Mechanism Able to Adapt the Energetic Cost of the Contraction to the Task

  • PhysioLab, University of Florence, Florence, Italy

A dual regulation of contraction operates in both skeletal and cardiac muscles. The first mechanism, based on Ca2+-dependent structural changes of the regulatory proteins in the thin filament, makes the actin sites available for binding of the myosin motors. The second recruits the myosin heads from the OFF state, in which they are unable to split ATP and bind to actin, in relation to the force during contraction. Comparison of the relevant X-ray diffraction signals marking the state of the thick filament demonstrates that the force feedback that controls the regulatory state of the thick filament works in the same way in skeletal as in cardiac muscles: even if in an isometric tetanus of skeletal muscle force is under the control of the firing frequency of the motor unit, while in a heartbeat force is controlled by the afterload, the stress-sensor switching the motors ON plays the same role in adapting the energetic cost of the contraction to the force. A new aspect of the Frank-Starling law of the heart emerges: independent of the diastolic filling of the ventricle, the number of myosin motors switched ON during systole, and thus the energetic cost of contraction, are tuned to the arterial pressure. Deterioration of the thick-filament regulation mechanism may explain the hyper-contractility related to hypertrophic cardiomyopathy, an inherited heart disease that in 40% of cases is due to mutations in cardiac myosin.

Introduction

In striated (skeletal and cardiac) muscles, the contractile machinery is organized in sarcomeres, 2-μm long structural units in which two antiparallel arrays of myosin motors from the thick filament generate steady force and shortening by cyclic ATP-driven interactions with the nearby thin actin-containing filaments originating from the opposite extremities of the sarcomere. According to the classical model of regulation of striated muscle, contraction is initiated by the increase of intracellular Ca2+-concentration ([Ca2+]i), induced by membrane depolarization by the action potential, followed by Ca2+-dependent structural changes in the regulatory proteins on the thin filament that release the actin sites for binding of the myosin motors (Ebashi et al., 1969; Huxley, 1973; Gordon et al., 2000). However, growing evidence that myosin motors in the resting muscle lie along the surface of the thick filament, folded towards the center of the sarcomere, unable to bind actin (Woodhead et al., 2005; Zoghbi et al., 2008) and hydrolyze ATP (Stewart et al., 2010), raised the question of how the motors can sense the state of the thin filament during activation. Using X-ray diffraction on intact myo-cells from skeletal and cardiac muscles at ID02 beamline of the European Synchrotron (ESRF, Grenoble, France) (Narayanan et al., 2017), a second regulatory mechanism, based on thick filament mechano-sensing, has been identified, which controls the recruitment of myosin motors from the state at rest in relation to the load (Linari et al., 2015; Reconditi et al., 2017).

Dual Filament Regulation in the Skeletal Muscle

In a tetanic contraction of skeletal muscle (Figure 1A), the thin filament is kept activated by the maintained high level of [Ca2+]i induced by repetitive firing of action potentials (Caputo et al., 1994). [Ca2+]i raises from the resting level (<10-7 M) to a maximum (∼10-5 M) within 10 ms from the first action potential, which correspond to the latent period for the mechanical response (Figure 1A,a), inducing a rapid structural change in the regulatory troponin-tropomyosin complex on the thin filament that exposes actin sites for binding with myosin motors (Kress et al., 1986; Gordon et al., 2000). Attachment of myosin motors to the actin filament can be structurally characterized using X-ray diffraction in intact muscle cells. By exploiting X-ray interference between the two arrays of myosin motors in each thick filament (Linari et al., 2000), it was found that changes in the fine structure of the M3 meridional reflection, originating from the 14.5-nm axial repeat of myosin motors along the thick filament, indicate a 10-nm movement of the center of mass of the myosin motors during the transition from the resting OFF state, in which they lie on the surface of the thick filament (Figure 1B, blue), to the actin-attached state characteristic of the isometric contraction (Figure 1B, red) (Huxley et al., 2006; Reconditi et al., 2011, 2014). The structural changes marking thick filament activation, such as the intensity drop of the first myosin layer line reflection (ML1) that records the loss of the three-stranded helical symmetry when myosin motors switch ON (Figure 1B, gray), and the ∼1.5% spacing increase of the sixth order meridional reflection (M6) that records the increase in the extension of filament backbone (half-time ∼25 ms), are two times slower than Ca2+-dependent thin filament activation, but lead myosin motor attachment and force generation (half-time ∼50 ms; Brunello et al., 2006; Reconditi et al., 2011). However, skeletal muscle can shorten at the maximum velocity (V0, the velocity under zero load) at the end of the latent period (Lombardi and Menchetti, 1984), when the thin filament is fully activated by Ca2+ but the thick filament is still OFF (Linari et al., 2015). This somewhat surprising finding is supported by recent mechanical experiments showing that very few myosin motors (≤3) per half-thick filament are enough to sustain V0 shortening (Fusi et al., 2017). Most importantly, V0 shortening imposed at the end of the latent period to prevent force development maintains the OFF structure of the thick filament, even if [Ca2+]i is high (Linari et al., 2015). Moreover, if V0 shortening is superimposed to the plateau of an isometric tetanus (T0, Figure 1A,b), when the thick filament is fully ON, to drop and keep force to zero (Figure 1A,c), the OFF state is progressively recovered. Accordingly, the rate of force redevelopment following the end of V0 shortening is lower the longer the duration of V0 shortening. Thus, thick filament regulatory state determines the rate of force development and in turn depends on the force acting on the filament by means of a positive feedback that rapidly adapts the number of available motors to the load.

FIGURE 1
www.frontiersin.org

FIGURE 1. Mechanical responses in skeletal and cardiac muscle and schematic of myosin motor configurations. (A) Force (upper panel) and half-sarcomere length change (lower panel) as measured by the striation follower (Huxley et al., 1981) during a tetanic stimulation of a single fiber dissected from frog muscle (at 4°C and SL 2.15 μm). The small bar below the force trace indicates the time of the first stimulus. a: latency period; b: plateau of force attained in isometric conditions; c: phase in which a shortening at V0 is imposed on the fiber. (B) Schematic of the motor conformation in various states: blue, OFF state; red, attached force-generating motor; orange, detached partner of the attached force-generating motor; gray, detached dimer in the ON state. (C) Pressure–volume loops with two different diastolic filling (preload): blue, high preload; red, low preload. The straight line describes the end-systolic pressure–end-systolic volume (ESPV) relation. (D) Relation between force at the peak of the twitch and sarcomere length in a cardiac trabecula at 2.5 mM Ca2+ and 27°C. Gray circles, FE conditions; black circles, LC conditions. The dashed line is the exponential fit to the experimental points. The two loops are ideal pressure–volume loops drawn assuming that two isometric contractions start at the same SL (2.2 μm) and become isotonic at two different levels of force corresponding to two different points in the ESPV relation depending on the blood pressure. (E) Force (upper panel) and half-sarcomere length changes (lower panel) during a trabecula twitch under fixed-end (gray) and length clamp (black) conditions at 2.5 mM Ca2+ and 27°C.

Dual Filament Regulation in the Cardiac Muscle

Shortly after the discovery of mechano-sensing-based thick filament regulation in skeletal muscle, a similar mechanism was found to operate in the heart (Reconditi et al., 2017). The heart mechanical activity (systole) consists in short periodic contractions (twitches) triggered by single action potentials. During systole the blood is pumped by ventricles into the arterial circulation. In the resting period between two systoles (diastole), the heart is filled by the blood from the venous return. In contrast to skeletal muscle, in cardiac muscle [Ca2+]i may not reach the level for full thin filament activation during systole. Consequently, the mechanical response depends on both [Ca2+]i and Ca2+-sensitivity of the filament (Allen and Kentish, 1985; ter Keurs, 2012), parameters that are under the control of several regulatory systems. These are either intrinsic, like the relation between sarcomere length (SL) and systolic force (a property known as Length Dependent Activation, LDA) (Sagawa et al., 1988; de Tombe et al., 2010), or extrinsic, like neuro-humoral control of the degree of phosphorylation of contractile, regulatory, and cytoskeletal proteins (Hidalgo and Granzier, 2013; Kumar et al., 2015; Kampourakis et al., 2016). LDA is the cellular basis of the Frank–Starling law of the heart that, in its classical formulation, relates the pressure exerted on the blood during the contraction of the ventricle (end-systolic pressure) to its filling during the relaxation (end-diastolic volume) (Figure 1C), in this way ensuring the dynamic equilibrium between the two circulatory systems (pulmonary and systemic) driven by two pumps in series. As shown in Figure 1C, the loop starting from a larger end-diastolic volume of the ventricle (blue with respect to red) attains a higher afterload during the isovolumetric phase of systole, so that the isotonic shortening terminates with a higher end-systolic pressure intersecting the end systolic pressure-volume (ESPV) relation (black line) at a higher end-systolic volume. In turn, the ESPV relation defines the points at which the contracting cardiac cells are neither lengthening nor shortening, representing the organ correlate of the active tension–length relation at sarcomere level (Figure 1D, dashed line) (Arts et al., 1991).

How thick filament mechano-sensing is integrated with the peculiar properties of the heart to modulate its performance was investigated by using X-ray diffraction on electrically paced intact trabeculae, pillar-like multicellular preparations dissected from the internal wall of the ventricle of rat heart. Trabecula attachments to the transducer levers entail an end compliance that during force development in a twitch in fixed-end conditions (FE) causes 10–15% shortening of the sarcomeres (Figure 1E, gray trace). Shortening can be prevented by feeding it forward to the loudspeaker motor in the next twitch (length-clamp condition, LC, Figure 1E, black trace). The relation between peak-twitch force (Tp) and SL is uniquely determined by the SL at Tp (black-LC and gray-FE circles lie on the same relation in Figure 1D) (Caremani et al., 2016). At the ID02 beamline the possibility to rapidly change the length of the camera from 0.6 to 31 m allows recording of both the meridional reflections from the nanometer-scale assembly of the contractile proteins in the filaments and the micrometer-scale SL. The X-ray signals marking the state of the thick filament show that in diastole the myosin motors are in the same OFF state as that of resting skeletal muscle, while during systole only a fraction of the motors leaves the OFF state, depending on the loading conditions (Reconditi et al., 2017).

Comparative Analysis of the Mechano-Sensing-Dependent State of the Thick Filament

Does the positive feedback between force and thick filament activation work in the same way in skeletal and cardiac muscles? This question is analyzed in Figure 2 by comparing the force-dependence of the relevant parameters marking the state of the filament, that is the intensity of ML1, IML1 (A), the spacing of M6, SM6 (B) and the fraction of OFF motors, fOFF (C), calculated from the structural model simulation of the intensity and fine structure of the M3 reflection (D) (Reconditi et al., 2011, 2017). The model assumes that the axial mass distribution responsible for the M3 reflection is given by the contribution of three populations: (i) motors in the OFF state lying on the surface of the thick filament folded back towards the center of the sarcomere (blue in Figure 1B); (ii) attached force-generating motors (red); (iii) detached motors made by the partners of attached motors (orange), and detached dimers not significantly contributing to the M3 intensity for their large conformational dispersion (gray). In skeletal muscle during isometric tetanus development the changes of both IML1 (Figure 2A, filled circles) and SM6 (Figure 2B, filled circles) are almost complete when the force on the thick filament has attained ∼220 pN (∼0.5 T0). fOFF (Figure 2C, filled circles), instead, drops in proportion to force rise, attaining zero at ∼400 pN. In the trabecula both IML1 (Figure 2A, open squares) and SM6 (Figure 2B, open squares) change with Tp in the same way as during force development in the skeletal muscle. Also, fOFF (Figure 2C, open squares) decreases in proportion to force increase, but with a slightly steeper slope, as if the force feedback for switching motors ON had a larger gain than in skeletal muscle.

FIGURE 2
www.frontiersin.org

FIGURE 2. Comparison of force-dependence of the relevant parameters marking the regulatory state of the thick filament. Filled circles: data from force development of isometric tetanus of single muscle fibers; open squares: data from Tp of trabeculae in FE or LC conditions. Force per thick filament in skeletal muscle fiber and heart trabecula is calculated from the force per cross-sectional area reported in the original papers, taking into account the fractional occupancy of the myofibrils, 0.83 in frog skeletal muscle (Mobley and Eisenberg, 1975) and 0.61 in rat trabeculae (Schaper et al., 1985; Barth et al., 1992), and the filament lattice spacing that is the same in both muscles (Irving et al., 2000; Reconditi et al., 2014). The calculated density of thick filaments is 5.87.1014 m-2 and 4.31.1014 m-2 in the skeletal muscle fiber and in the trabecula respectively. (A) IML1, intensity of ML1 reflection. Filled circles from Figure 4B in Reconditi et al., 2011; open squares from Figure S2C in Reconditi et al., 2017. (B) SM6, spacing of M6 reflection. Filled circles from Figure 3d in Linari et al., 2015; open squares from Figure 2F in Reconditi et al., 2017. (C) fOFF, fraction of motors in the OFF state. Filled circles from Figure 3D in Reconditi et al., 2011; open squares from Table 1 in Reconditi et al., 2017. (D) Comparison of the intensity profiles of the M3 reflection in skeletal muscle (a, from Figure 3C in Reconditi et al., 2011; thick black trace, rest; thick blue trace, T0; red lines, outputs of the structural model) and cardiac muscle (b, from Figure 2C in Reconditi et al., 2017; blue trace, diastole; violet and red traces, high force and low force twitch, respectively). The intensity profile of the low force twitch (red trace in b) is solely explained by including the contribution from a substantial fraction of the myosin motors in the OFF conformation. (E) Fraction of actin-attached motors (fA). Filled circles and open squares as in (C). (F) rc, duty ratio. Filled circles calculated from filled circles in (C) and (E). Open squares from Table 1 in Reconditi et al., 2017.

Sarcomere-level mechanics allows the comparison to be extended to two functional parameters of the regulatory state of the thick filament: the fraction of actin-attached motors fA (Figure 2E), and the duty ratio rc (the ratio between fA and the fraction of motors available for actin interaction, 1-fOFF) (Figure 2F). fA increases in proportion to the isometric force in both skeletal (filled circles) and cardiac (open squares) muscles (Brunello et al., 2006; Fusi et al., 2010; Pinzauti et al., 2018). The duty ratio rc, instead, results independent of the force, and thus of the regulatory state of the thick filament, in both muscle fibers (filled circles) and trabeculae (open squares). This result is not surprising: in fact, in either case, the increase in fA occurs without change in the strain per attached motor and thus, in terms of Huxley, 1957 two-state model, without change in the fraction of the ATPase cycle time each motor spends attached to the actin. We conclude that force controls the regulatory state of the thick filament modulating the number of available motors and not the kinetics of motor attachment to actin (Irving, 2017).

Molecular Bases of Thick Filament Mechano-Sensing

The structural and functional parameters related to thick filament regulation reported in Figure 2 depend on the force in a similar way in skeletal and cardiac muscle, through the action of a common, still unknown, mechano-sensor. The finding that the switching ON of the motors is accompanied by an increase in the backbone axial periodicity one order of magnitude larger than that due to filament compliance, strongly supports the idea that the common mechanism is based on the stress-sensitivity of the intermolecular–intramolecular interactions determining the helical packing of myosin motors in the OFF state. These interactions are generated not only within the myosin molecules (head–head and head–tail interactions responsible for what is called the interacting head motif, IHM; Alamo et al., 2008), but also with other thick filament proteins like titin and myosin binding protein C (MyBP-C) that are assembled so as to match the 43-nm helical periodicity of the myosin molecules (Rome et al., 1973; Labeit et al., 1992). The stress-dependent disruption of these interactions would determine the release of myosin motors from the surface of the filament with loss of the helical order and 1.5% extension of the backbone (Huxley and Brown, 1967; Irving, 2017). A role as a mechano-sensor has been attributed to titin, which spans the whole half-sarcomere, connecting the Z-line at the end of the sarcomere with the tip of the myosin filament and then running, bound to the surface of the thick filament, up to the M-line at the center of the sarcomere. Titin can transmit the stress to thick filament also in the resting sarcomere, when no motors are attached to actin, as recently demonstrated in relaxed skinned fibers from mammalian skeletal muscle (Fusi et al., 2016), in which, however, the features of the resting state of the intact cell are only partially preserved (Xu et al., 2009; Caremani et al., 2017). The hypothesis of titin as a mechano-sensor is further supported by the finding that titin stiffness increases with the increase in [Ca2+] (Labeit et al., 2003), but is weakened by the evidence that the titin mediated stress of the thick filament has been proved only at SL > 2.8 μm. MyBP-C is bound with its C-terminal to the backbone of the thick filament in the central one-third of the half-sarcomere (C-zone) and extends from the thick filament to establish, with its N-terminal, dynamic interactions, controlled by the level of phosphorylation, with either the actin filament or the rod-like S2 domain of the myosin (Moos et al., 1978; Rybakova et al., 2011; Pfuhl and Gautel, 2012). In cardiac MyBP-C, a supplementary N-terminal domain, the C0 domain, dynamically interacts with either the actin or the regulatory light chain (RLC) in the myosin head. MyBP-C is the most likely interfilament signaling protein able to affect the IHM (Kampourakis et al., 2014; Harris et al., 2016; Kensler et al., 2017). In the intact muscle fiber the resting viscosity (likely related to inter-filamentary links) disappears at the end of latent period, when the fiber becomes able to shorten at V0 (Lombardi and Menchetti, 1984). Noteworthy in the cardiac cell the development of V0-shortening is much slower and is completed when force attains 50% of the maximum twitch force (de Tombe and ter Keurs, 1992), suggesting different dynamics for the disappearance of the internal load.

Another actor in thick filament regulation, specific of the cardiac myocyte, is the basic elevated degree of phosphorylation of the RLC (40%, Toepfer et al., 2013); accordingly, disruption of the OFF state depends on RLC phosphorylation (Kampourakis and Irving, 2015). The phosphorylation-dependent tuning by MyBP-C and RLC of the thick filament state in cardiac muscle might explain the larger gain in the force feedback that switches motors ON in cardiac muscle with respect to skeletal muscle (Figure 2C).

Mutations in the aminoacids on the surface of the S1 domain are causative of hypertrophic cardiomyopathy (HCM), an inherited heart disease characterized by thickening of the ventricular wall that diminishes the relaxation capacity and ventricular filling. In the folded IHM state, the surface of the S1 domain presents binding sites for the proximal S2 domain of the myosin and for the MyBP-C (Spudich, 2015; Alamo et al., 2017; Trivedi et al., 2018) and mutations in this region (the myosin mesa) would weaken the interactions responsible for the IHM, leading to the hyper-contractility associated with HCM. The demonstration of stress-sensing in thick filament of cardiac muscle opens a new scenario in which the HCM-causing mutations in the myosin mesa produce hyper-contractility by reducing the force threshold of the switch that controls the fraction of motors in the ON state.

Role of Thick Filament Mechano-Sensing in Skeletal and Cardiac Muscles

In spite of the strict similarities of thick filament mechano-sensing in skeletal and cardiac muscles, the mechanism is integrated in peculiar ways with the function of these muscles. In the skeletal muscle, during the high firing frequency that sustains maximum tetanic force T0, mechano-sensing in the thick filament activation speeds up force development during high load contraction (Linari et al., 2015). However, voluntary movements during the physiological activity of skeletal muscle may imply lower firing frequencies and consequently sub-tetanic forces that can be even lower than 0.5 T0 (Macefield et al., 1996). In this case, thick filament mechano-sensing provides partial activation (Figures 2A–C), revealing a supplementary energetic gain in the tuning of contraction by the firing frequency of the nerve. In addition, thick filament mechano-sensing explains the reduction of ATP utilization below the value expected from solution kinetics measurements if the contraction occurs at very low load (Homsher et al., 1981; Fusi et al., 2017).

In a heartbeat, the whole contraction is submaximal and the force generated during systole varies in a range within which, as shown by open squares in Figure 2, a given fraction of motors remains in the OFF state. In this case, the positive feedback between force and thick filament activation operates to adapt the switched ON motors to the load, independent of the diastolic sarcomere length. The LC and FE twitches in Figure 1E approximate the conditions of the left ventricle beating against a high (LC twitch) and a low (FE twitch) aortic pressure. In turn, the ESPV relation of the left ventricle (Figure 1C, continuous line) is the organ correlate of the active force–SL relation (Figure 1D dashed line). The two ideal loops in Figure 1D represent contractions that start at 2.2 μm SL and become isotonic when the force attains the level identified by the intercept on the relation (blue: high load, red: low load). At organ level they correspond to two pressure-volume loops with the same preload (end-diastolic volume) and different afterloads (aortic pressures), providing a new view of the Frank–Starling mechanism: independent of the diastolic filling of the ventricle, the recruitment of myosin motors and thus the energetic cost of systole is tuned to the load, that is to the aortic pressure.

Perspectives

Future work, aimed at identifying the molecular basis of thick filament mechano-sensing, acquires particular relevance in cardiac muscle in relation to the Ca2+-dependent thin filament activation and to the destabilizing action of the phosphorylation of the proteins contributing to the OFF state of the motor. The discovery of mechano-sensing in the thick filament implies that the hyper-contractility accompanying HCM-causing mutations in these proteins would result not only from a dysregulation of their degree of phosphorylation but also from an alteration of the gain of the positive feedback between force on the thick filament and motor recruitment. Understanding the molecular basis of the mechano-sensing controlling the regulatory state of the thick filament is a prerequisite in drug development for specific therapeutic interventions.

Author Contributions

GP, MC, ML, MR, and VL wrote and edited the manuscript.

Conflict of Interest Statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Acknowledgments

The authors wish to thank the Fondazione Cassa di Risparmio di Firenze, Italy, for the financial support to the research. The authors thank Theyencheri Narayanan and his staff at beamline ID02 of the European Synchrotron ESRF (Grenoble, France) for the unique opportunity to perform nanometer-micrometer range X-ray diffraction.

References

Alamo, L., Ware, J. S., Pinto, A., Gillilan, R. E., Seidman, J. G., Seidman, C. E., et al. (2017). Effects of myosin variants on interacting-heads motif explain distinct hypertrophic and dilated cardiomyopathy phenotypes. eLife 6:e24634. doi: 10.7554/eLife.24634

PubMed Abstract | CrossRef Full Text | Google Scholar

Alamo, L., Wriggers, W., Pinto, A., Bartoli, F., Salazar, L., Zhao, F. Q., et al. (2008). Three-dimensional reconstruction of tarantula myosin filaments suggests how phosphorylation may regulate myosin activity. J. Mol. Biol. 384, 780–797. doi: 10.1016/j.jmb.2008.10.013

PubMed Abstract | CrossRef Full Text | Google Scholar

Allen, D. G., and Kentish, J. C. (1985). The cellular basis of the length-tension relation in cardiac muscle. J. Mol. Cell Cardiol. 17, 821–840. doi: 10.1016/S0022-2828(85)80097-3

CrossRef Full Text | Google Scholar

Arts, T., Bovendeerd, P. H., Prinzen, F. W., and Reneman, R. S. (1991). Relation between left ventricular cavity pressure and volume and systolic fiber stress and strain in the wall. Biophys. J. 59, 93–102. doi: 10.1016/S0006-3495(91)82201-9

PubMed Abstract | CrossRef Full Text | Google Scholar

Barth, E., Stammler, G., Speiser, B., and Schaper, J. (1992). Ultrastructural quantitation of mitochondria and myofilaments in cardiac muscle from 10 different animal species including man. J. Mol. Cell Cardiol. 24, 669–681. doi: 10.1016/0022-2828(92)93381-S

PubMed Abstract | CrossRef Full Text | Google Scholar

Brunello, E., Bianco, P., Piazzesi, G., Linari, M., Reconditi, M., Panine, P., et al. (2006). Structural changes in the myosin filament and cross-bridges during active force development in single intact frog muscle fibres: stiffness and X-ray diffraction measurements. J. Physiol. 577, 971–984. doi: 10.1113/jphysiol.2006.115394

PubMed Abstract | CrossRef Full Text | Google Scholar

Caputo, C., Edman, K. A., Lou, F., and Sun, Y. B. (1994). Variation in myoplasmic Ca2+ concentration during contraction and relaxation studied by the indicator fluo-3 in frog muscle fibres. J. Physiol. 478(Pt 1), 137–148. doi: 10.1113/jphysiol.1994.sp020237

PubMed Abstract | CrossRef Full Text | Google Scholar

Caremani, M., Fusi, L., Reconditi, M., Piazzesi, G., Narayanan, T., Irving, M., et al. (2017). Structural changes in the thick filaments during activation of demembranated skeletal muscle fibers. Biophys. J. 112:181a. doi: 10.1016/j.bpj.2016.11.1004

CrossRef Full Text | Google Scholar

Caremani, M., Pinzauti, F., Reconditi, M., Piazzesi, G., Stienen, G. J., Lombardi, V., et al. (2016). Size and speed of the working stroke of cardiac myosin in situ. Proc. Natl. Acad. Sci. U.S.A. 113, 3675–3680. doi: 10.1073/pnas.1525057113

PubMed Abstract | CrossRef Full Text | Google Scholar

de Tombe, P. P., Mateja, R. D., Tachampa, K., Ait Mou, Y., Farman, G. P., and Irving, T. C. (2010). Myofilament length dependent activation. J. Mol. Cell Cardiol. 48, 851–858. doi: 10.1016/j.yjmcc.2009.12.017

PubMed Abstract | CrossRef Full Text | Google Scholar

de Tombe, P. P., and ter Keurs, H. E. (1992). An internal viscous element limits unloaded velocity of sarcomere shortening in rat myocardium. J. Physiol. 454, 619–642. doi: 10.1113/jphysiol.1992.sp019283

PubMed Abstract | CrossRef Full Text | Google Scholar

Ebashi, S., Endo, M., and Otsuki, I. (1969). Control of muscle contraction. Q. Rev. Biophys. 2, 351–384. doi: 10.1017/S0033583500001190

CrossRef Full Text | Google Scholar

Fusi, L., Brunello, E., Yan, Z., and Irving, M. (2016). Thick filament mechano-sensing is a calcium-independent regulatory mechanism in skeletal muscle. Nat. Commun. 7:13281. doi: 10.1038/ncomms13281

PubMed Abstract | CrossRef Full Text | Google Scholar

Fusi, L., Percario, V., Brunello, E., Caremani, M., Bianco, P., Powers, J. D., et al. (2017). Minimum number of myosin motors accounting for shortening velocity under zero load in skeletal muscle. J. Physiol. 595, 1127–1142. doi: 10.1113/JP273299

PubMed Abstract | CrossRef Full Text | Google Scholar

Fusi, L., Reconditi, M., Linari, M., Brunello, E., Elangovan, R., Lombardi, V., et al. (2010). The mechanism of the resistance to stretch of isometrically contracting single muscle fibres. J. Physiol. 588, 495–510. doi: 10.1113/jphysiol.2009.178137

PubMed Abstract | CrossRef Full Text | Google Scholar

Gordon, A. M., Homsher, E., and Regnier, M. (2000). Regulation of contraction in striated muscle. Physiol. Rev. 80, 853–924. doi: 10.1152/physrev.2000.80.2.853

PubMed Abstract | CrossRef Full Text | Google Scholar

Harris, S. P., Belknap, B., Van Sciver, R. E., White, H. D., and Galkin, V. E. (2016). C0 and C1 N-terminal Ig domains of myosin binding protein C exert different effects on thin filament activation. Proc. Natl. Acad. Sci. U.S.A. 113, 1558–1563. doi: 10.1073/pnas.1518891113

PubMed Abstract | CrossRef Full Text | Google Scholar

Hidalgo, C., and Granzier, H. (2013). Tuning the molecular giant titin through phosphorylation: role in health and disease. Trends Cardiovasc. Med. 23, 165–171. doi: 10.1016/j.tcm.2012.10.005

PubMed Abstract | CrossRef Full Text | Google Scholar

Homsher, E., Irving, M., and Wallner, A. (1981). High-energy phosphate metabolism and energy liberation associated with rapid shortening in frog skeletal muscle. J. Physiol. 321, 423–436. doi: 10.1113/jphysiol.1981.sp013994

PubMed Abstract | CrossRef Full Text | Google Scholar

Huxley, A. F. (1957). Muscle structure and theories of contraction. Prog. Biophys. Biophys. Chem. 7, 255–318.

Google Scholar

Huxley, A. F. (1973). A note suggesting that the cross-bridge attachment during muscle contraction may take place in two stages. Proc. R. Soc. Lond. B Biol. Sci. 183, 83–86. doi: 10.1098/rspb.1973.0006

PubMed Abstract | CrossRef Full Text | Google Scholar

Huxley, A. F., Lombardi, V., and Peachey, D. (1981). A system for fast recording of longitudinal displacement of a striated muscle fibre. J. Physiol. 317, 12–13.

Google Scholar

Huxley, H. E., and Brown, W. (1967). The low-angle x-ray diagram of vertebrate striated muscle and its behaviour during contraction and rigor. J. Mol. Biol. 30, 383–434. doi: 10.1016/S0022-2836(67)80046-9

PubMed Abstract | CrossRef Full Text | Google Scholar

Huxley, H. E., Reconditi, M., Stewart, A., and Irving, T. (2006). X-ray interference studies of crossbridge action in muscle contraction: evidence from quick releases. J. Mol. Biol. 363, 743–761. doi: 10.1016/j.jmb.2006.08.075

PubMed Abstract | CrossRef Full Text | Google Scholar

Irving, M. (2017). Regulation of contraction by the thick filaments in skeletal muscle. Biophys. J. 113, 2579–2594. doi: 10.1016/j.bpj.2017.09.037

PubMed Abstract | CrossRef Full Text | Google Scholar

Irving, T. C., Konhilas, J., Perry, D., Fischetti, R., and De Tombe, P. P. (2000). Myofilament lattice spacing as a function of sarcomere length in isolated rat myocardium. Am. J. Physiol. Heart Circ. Physiol. 279, H2568–H2573. doi: 10.1152/ajpheart.2000.279.5.H2568

PubMed Abstract | CrossRef Full Text | Google Scholar

Kampourakis, T., and Irving, M. (2015). Phosphorylation of myosin regulatory light chain controls myosin head conformation in cardiac muscle. J. Mol. Cell Cardiol. 85, 199–206. doi: 10.1016/j.yjmcc.2015.06.002

PubMed Abstract | CrossRef Full Text | Google Scholar

Kampourakis, T., Sun, Y. B., and Irving, M. (2016). Myosin light chain phosphorylation enhances contraction of heart muscle via structural changes in both thick and thin filaments. Proc. Natl. Acad. Sci. U.S.A. 113, E3039–E3047. doi: 10.1073/pnas.1602776113

PubMed Abstract | CrossRef Full Text | Google Scholar

Kampourakis, T., Yan, Z., Gautel, M., Sun, Y. B., and Irving, M. (2014). Myosin binding protein-C activates thin filaments and inhibits thick filaments in heart muscle cells. Proc. Natl. Acad. Sci. U.S.A. 111, 18763–18768. doi: 10.1073/pnas.1413922112

PubMed Abstract | CrossRef Full Text | Google Scholar

Kensler, R. W., Craig, R., and Moss, R. L. (2017). Phosphorylation of cardiac myosin binding protein C releases myosin heads from the surface of cardiac thick filaments. Proc. Natl. Acad. Sci. U.S.A. 114, E1355–E1364. doi: 10.1073/pnas.1614020114

PubMed Abstract | CrossRef Full Text | Google Scholar

Kress, M., Huxley, H. E., Faruqi, A. R., and Hendrix, J. (1986). Structural changes during the activation of frog muscle studied by time-resolved x-ray diffraction. J. Mol. Biol. 188, 325–342. doi: 10.1016/0022-2836(86)90158-0

CrossRef Full Text | Google Scholar

Kumar, M., Govindan, S., Zhang, M., Khairallah, R. J., Martin, J. L., Sadayappan, S., et al. (2015). Cardiac myosin-binding protein C and Troponin-I phosphorylation independently modulate myofilament length-dependent activation. J. Biol. Chem. 290, 29241–29249. doi: 10.1074/jbc.M115.686790

PubMed Abstract | CrossRef Full Text | Google Scholar

Labeit, D., Watanabe, K., Witt, C., Fujita, H., Wu, Y., Lahmers, S., et al. (2003). Calcium-dependent molecular spring elements in the giant protein titin. Proc. Natl. Acad. Sci. U.S.A. 100, 13716–13721. doi: 10.1073/pnas.2235652100

PubMed Abstract | CrossRef Full Text | Google Scholar

Labeit, S., Gautel, M., Lakey, A., and Trinick, J. (1992). Towards a molecular understanding of titin. EMBO J. 11, 1711–1716.

Google Scholar

Linari, M., Brunello, E., Reconditi, M., Fusi, L., Caremani, M., Narayanan, T., et al. (2015). Force generation by skeletal muscle is controlled by mechanosensing in myosin filaments. Nature 528, 276–279. doi: 10.1038/nature15727

PubMed Abstract | CrossRef Full Text | Google Scholar

Linari, M., Piazzesi, G., Dobbie, I., Koubassova, N., Reconditi, M., Narayanan, T., et al. (2000). Interference fine structure and sarcomere length dependence of the axial X-ray pattern from active single muscle fibers. Proc. Natl. Acad. Sci. U.S.A. 97, 7226–7231. doi: 10.1073/pnas.97.13.7226

PubMed Abstract | CrossRef Full Text | Google Scholar

Lombardi, V., and Menchetti, G. (1984). The maximum velocity of shortening during the early phases of the contraction in frog single muscle fibres. J. Muscle Res. Cell Motil. 5, 503–513. doi: 10.1007/BF00713257

CrossRef Full Text | Google Scholar

Macefield, V. G., Fuglevand, A. J., and Bigland-Ritchie, B. (1996). Contractile properties of single motor units in human toe extensors assessed by intraneural motor axon stimulation. J. Neurophysiol. 75, 2509–2519. doi: 10.1152/jn.1996.75.6.2509

PubMed Abstract | CrossRef Full Text | Google Scholar

Mobley, B. A., and Eisenberg, B. R. (1975). Sizes of components in frog skeletal muscle measured by methods of stereology. J. Gen. Physiol. 66, 31–45. doi: 10.1085/jgp.66.1.31

PubMed Abstract | CrossRef Full Text | Google Scholar

Moos, C., Mason, C. M., Besterman, J. M., Feng, I. N., and Dubin, J. H. (1978). The binding of skeletal muscle C-protein to F-actin, and its relation to the interaction of actin with myosin subfragment-1. J. Mol. Biol. 124, 571–586. doi: 10.1016/0022-2836(78)90172-9

PubMed Abstract | CrossRef Full Text | Google Scholar

Narayanan, T., Wacklin, H., Konovalov, O., and Lund, R. (2017). Recent applications of synchrotron radiation and neutrons in the study of soft matter. Crystallogr. Rev. 23, 160–226. doi: 10.1080/0889311X.2016.1277212

CrossRef Full Text | Google Scholar

Pfuhl, M., and Gautel, M. (2012). Structure, interactions and function of the N-terminus of cardiac myosin binding protein C (MyBP-C): who does what, with what, and to whom? J. Muscle Res. Cell Motil. 33, 83–94. doi: 10.1007/s10974-012-9291-z

PubMed Abstract | CrossRef Full Text | Google Scholar

Pinzauti, F., Pertici, I., Reconditi, M., Narayanan, T., Stienen, G. J. M., Piazzesi, G., et al. (2018). The force and stiffness of myosin motors in the isometric twitch of a cardiac trabecula and the effect of the extracellular Calcium concentration. J. Physiol. doi: 10.1113/JP275579 [Epub ahead of print].

PubMed Abstract | CrossRef Full Text | Google Scholar

Reconditi, M., Brunello, E., Fusi, L., Linari, M., Martinez, M. F., Lombardi, V., et al. (2014). Sarcomere-length dependence of myosin filament structure in skeletal muscle fibres of the frog. J. Physiol. 592, 1119–1137. doi: 10.1113/jphysiol.2013.267849

CrossRef Full Text | Google Scholar

Reconditi, M., Brunello, E., Linari, M., Bianco, P., Narayanan, T., Panine, P., et al. (2011). Motion of myosin head domains during activation and force development in skeletal muscle. Proc. Natl. Acad. Sci. U.S.A. 108, 7236–7240. doi: 10.1073/pnas.1018330108

PubMed Abstract | CrossRef Full Text | Google Scholar

Reconditi, M., Caremani, M., Pinzauti, F., Powers, J. D., Narayanan, T., Stienen, G. J., et al. (2017). Myosin filament activation in the heart is tuned to the mechanical task. Proc. Natl. Acad. Sci. U.S.A. 114, 3240–3245. doi: 10.1073/pnas.1619484114

PubMed Abstract | CrossRef Full Text | Google Scholar

Rome, E., Offer, G., and Pepe, F. A. (1973). X-ray diffraction of muscle labelled with antibody to C-protein. Nat. New Biol. 244, 152–154. doi: 10.1038/newbio244152a0

PubMed Abstract | CrossRef Full Text | Google Scholar

Rybakova, I. N., Greaser, M. L., and Moss, R. L. (2011). Myosin binding protein C interaction with actin: characterization and mapping of the binding site. J. Biol. Chem. 286, 2008–2016. doi: 10.1074/jbc.M110.170605

PubMed Abstract | CrossRef Full Text | Google Scholar

Sagawa, K., Maughan, W. L., Suga, H., and Sunagawa, K. (1988). Cardiac Contraction and the Pressure-Volume Relationship. New York, NY: Oxford University Press.

Google Scholar

Schaper, J., Meiser, E., and Stammler, G. (1985). Ultrastructural morphometric analysis of myocardium from dogs, rats, hamsters, mice, and from human hearts. Circ. Res. 56, 377–391. doi: 10.1161/01.RES.56.3.377

PubMed Abstract | CrossRef Full Text | Google Scholar

Spudich, J. A. (2015). The myosin mesa and a possible unifying hypothesis for the molecular basis of human hypertrophic cardiomyopathy. Biochem. Soc. Trans. 43, 64–72. doi: 10.1042/BST20140324

PubMed Abstract | CrossRef Full Text | Google Scholar

Stewart, M. A., Franks-Skiba, K., Chen, S., and Cooke, R. (2010). Myosin ATP turnover rate is a mechanism involved in thermogenesis in resting skeletal muscle fibers. Proc. Natl. Acad. Sci. U.S.A. 107, 430–435. doi: 10.1073/pnas.0909468107

PubMed Abstract | CrossRef Full Text | Google Scholar

ter Keurs, H. E. (2012). The interaction of Ca2+ with sarcomeric proteins: role in function and dysfunction of the heart. Am. J. Physiol. Heart Circ. Physiol. 302, H38–H50. doi: 10.1152/ajpheart.00219.2011

PubMed Abstract | CrossRef Full Text | Google Scholar

Toepfer, C., Caorsi, V., Kampourakis, T., Sikkel, M. B., West, T. G., Leung, M. C., et al. (2013). Myosin regulatory light chain (RLC) phosphorylation change as a modulator of cardiac muscle contraction in disease. J. Biol. Chem. 288, 13446–13454. doi: 10.1074/jbc.M113.455444

PubMed Abstract | CrossRef Full Text | Google Scholar

Trivedi, D. V., Adhikari, A. S., Sarkar, S. S., Ruppel, K. M., and Spudich, J. A. (2018). Hypertrophic cardiomyopathy and the myosin mesa: viewing an old disease in a new light. Biophys. Rev. 10, 27–48. doi: 10.1007/s12551-017-0274-6

PubMed Abstract | CrossRef Full Text | Google Scholar

Woodhead, J. L., Zhao, F. Q., Craig, R., Egelman, E. H., Alamo, L., and Padron, R. (2005). Atomic model of a myosin filament in the relaxed state. Nature 436, 1195–1199. doi: 10.1038/nature03920

PubMed Abstract | CrossRef Full Text | Google Scholar

Xu, S., White, H. D., Offer, G. W., and Yu, L. C. (2009). Stabilization of helical order in the thick filaments by blebbistatin: further evidence of coexisting multiple conformations of myosin. Biophys. J. 96, 3673–3681. doi: 10.1016/j.bpj.2009.01.049

PubMed Abstract | CrossRef Full Text | Google Scholar

Zoghbi, M. E., Woodhead, J. L., Moss, R. L., and Craig, R. (2008). Three-dimensional structure of vertebrate cardiac muscle myosin filaments. Proc. Natl. Acad. Sci. U.S.A. 105, 2386–2390. doi: 10.1073/pnas.0708912105

PubMed Abstract | CrossRef Full Text | Google Scholar

Keywords: cardiac muscle regulation, skeletal muscle regulation, thick filament mechano-sensing, small angle X-ray diffraction, Frank-Starling law, myosin motor, duty ratio

Citation: Piazzesi G, Caremani M, Linari M, Reconditi M and Lombardi V (2018) Thick Filament Mechano-Sensing in Skeletal and Cardiac Muscles: A Common Mechanism Able to Adapt the Energetic Cost of the Contraction to the Task. Front. Physiol. 9:736. doi: 10.3389/fphys.2018.00736

Received: 30 March 2018; Accepted: 28 May 2018;
Published: 14 June 2018.

Edited by:

Kenneth S. Campbell, University of Kentucky, United States

Reviewed by:

Laurin Michelle Hanft, University of Missouri, United States
Douglas Root, University of North Texas, United States
Jonathan P. Davis, The Ohio State University, United States

Copyright © 2018 Piazzesi, Caremani, Linari, Reconditi and Lombardi. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

*Correspondence: Gabriella Piazzesi, gabriella.piazzesi@unifi.it