Original Research ARTICLE
A pilot study investigating the influence of glucagon-like peptide-1 receptor single nucleotide polymorphisms on gastric emptying rate in Caucasian men
- 1School of Healthcare Science, Manchester Metropolitan University, United Kingdom
- 2School of Medical Sciences, University of Manchester, United Kingdom
- 3School of Medicine, University of St Andrews, United Kingdom
- 4School of Biomedical Sciences, Ulster University, United Kingdom
Gastric emptying rate in humans is subject to large individual variability, but previous research on the influence of genetics is scarce. Variation in the glucagon-like peptide-1 receptor (GLP1R) gene is a plausible candidate gene to partially explain the high variance. This study aimed to investigate the influence of genetic variation in the GLP1R gene on gastric emptying rate of a glucose solution in humans. Forty eight healthy Caucasian males took part in this investigation. Gastric emptying rate of a 6% glucose solution was assessed using the 13C breath test method and a venous blood sample was obtained from each participant. Participants were genotyped for twenty-seven Tag single nucleotide polymorphisms (SNPs) in the GLP1R locus using Sequenom MassARRAY iPLEX GOLD analysis and MALDI-TOF mass spectrometry.
The time at which maximal emptying rate occurred (Tlag) was faster in participants with the CC genotype than in TT and TC genotypes for SNP rs742764: (Median (quartiles) CC, 35 (30-36) min vs. TT, 43 (39-46) min and TC, 41 (39-45) min; P < 0.01). Tlag was also slower in participants with the AA genotype compared to the TT and TA genotypes for SNP rs2254336: (AA, 43 (39-49) min vs. TT, 36 (34-41) min and TA, 39 (35-42) min; P < 0.05). Analysis by phenotype also showed differences in half-emptying time (T½) and Tlag for SNPs rs9283907, rs2268657 and rs2254336. Several neighbouring Tag SNPs within the GLP1R gene were found to be associated with gastric emptying rate, and should be further investigated.
Keywords: GLP-1r, Gastric emptying rate, Single nucleotide polymorphisms (SNPs), GLP-1, Gastrointestinal Motility
Received: 13 Mar 2018;
Accepted: 04 Sep 2018.
Edited by:Ravinder Abrol, California State University, Northridge, United States
Reviewed by:Matthias J. Bahr, Sana Kliniken Lübeck, Germany
Manlio Vinciguerra, International Clinical Research Center (FNUSA-ICRC), Czechia
Copyright: © 2018 Yau, Mclaughlin, Maughan, Gilmore, Ashworth and Evans. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Dr. Gethin H. Evans, Manchester Metropolitan University, School of Healthcare Science, Manchester, M1 5GD, United Kingdom, firstname.lastname@example.org