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Front. Physiol. | doi: 10.3389/fphys.2018.01399

Proteomic responses under cold stress and recovery reveal unique cold tolerant mechanism in the Pacific white shrimp (Litopenaeus vannamei)

Jin-Xia Peng1, Ping-Ping He1, Pin-Yuan Wei1, Bin Zhang1, Yong-Zhen Zhao1, Qiang-Yong Li1, Xiu-Li Chen1, Min Peng1, Di-Gang Zeng1, Chun-Ling Yang1 and  Xiaohan Chen1*
  • 1Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture,, Guangxi Academy of Fishery Sciences, China

The Pacific white shrimp (Litopenaeus vannamei), one of the most widely cultured shrimp species in the world, is often suffered from cold stress. In order to reveal the molecular mechanism of cold tolerance in the Pacific white shrimp, we conducted a proteomic analysis on two contrasting shrimp cultivars, namely, cold-tolerant Guihai2 (GH2) and cold-sensitive Guihai1 (GH1) under normal temperature 28°C, cold stress 16°C, and 16°C recovered to 28°C, respectively. In total, 3,349 proteins were identified, among which 2,736 proteins were quantified. Based on GO annotation, the main differentially expressed proteins were belonged to biological process, cellular component and molecular function. KEGG pathway annotation found that the main changes were in lysosome, ribosome, and oxidative phosphorylation, etc. Subcellular Localization analysis showed significant changes present in cytosol, extracellular and mitochondria, etc. Combining the enrichment-based clustering analysis and qRT-PCR analysis, we found that glutathione S-transferase, zinc proteinase, m7GpppX diphosphatase, AP2 transcription complex, and Zinc-finger transcription factors played a major role in cold stress response in the Pacific white shrimp. Moreover, structure proteins including different types of lectin and DAPPUDRAFT were indispensable for cold stress tolerance in the Pacific white shrimp. These results indicated molecular mechanism of Pacific white shrimp in response to cold stress and provided a new insight in breeding new cultivars with cold tolerance.

Keywords: Litopenaeus vannamei, proteomic, cold stress, transcription factor, qRT-PCR

Received: 25 May 2018; Accepted: 13 Sep 2018.

Edited by:

Youji Wang, Shanghai Ocean University, China

Reviewed by:

Jun Shi, University of Texas Southwestern Medical Center, United States
创举 李, Yangtze River Fisheries Research Institute, China
Xiaoxiang Liu, Hangzhou Medical College, China  

Copyright: © 2018 Peng, He, Wei, Zhang, Zhao, Li, Chen, Peng, Zeng, Yang and Chen. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Prof. Xiaohan Chen, Guangxi Academy of Fishery Sciences, Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture,, Nanning, 530021, Guangxi, China, chnxhn@163.com