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Front. Physiol. | doi: 10.3389/fphys.2018.01626

Properties of the Permeability Transition of Pea Stem Mitochondria

 Valentina De Col1,  Elisa Petrussa1, Valentino Casolo1,  Enrico Braidot1,  Giovanna Lippe1,  Antonio Filippi1, Carlo Peresson1, Sonia Patui1, Alberto Bertolini1,  Valentina Giorgio2, Vanessa Checchetto2, Angelo Vianello1,  Paolo Bernardi2 and  Marco Zancani1*
  • 1Università degli Studi di Udine, Italy
  • 2Università degli Studi di Padova, Italy

In striking analogy with Saccharomyces cerevisiae, etiolated pea stem mitochondria did not show appreciable Ca2+ uptake. Only treatment with the ionophore ETH129 (which allows electrophoretic Ca2+ equilibration) caused Ca2+ uptake followed by increased inner membrane permeability, membrane depolarization and Ca2+ release. Like the permeability transition (PT) of mammals, yeast and Drosophila, the PT of pea stem mitochondria was stimulated by diamide and phenylarsine oxide and inhibited by Mg-ADP and Mg-ATP, suggesting a common underlying mechanism; yet, the plant PT also displayed distinctive features: (i) as in mammals it was desensitized by cyclosporin A, which does not affect the PT of yeast and Drosophila; (ii) similarly to S. cerevisiae and Drosophila it was inhibited by Pi, which stimulates the PT of mammals; (iii) like in mammals and Drosophila it was sensitized by benzodiazepine 423, which is ineffective in S. cerevisiae; (iv) like what observed in Drosophila it did not mediate swelling and cytochrome c release, which is instead seen in mammals and S. cerevisiae. We find that cyclophilin D, the mitochondrial receptor for cyclosporin A, is present in pea stem mitochondria. These results indicate that the plant PT has unique features and suggest that, as in Drosophila, it may provide pea stem mitochondria with a Ca2+ release channel.

Keywords: Ca2+, Cyclophilin (CYP), Cyclosporin A, CsA, F-ATP synthase, permeability transition, Plant mitochondria

Received: 31 Jul 2018; Accepted: 29 Oct 2018.

Edited by:

Miguel A. Aon, National Institute on Aging (NIA), United States

Reviewed by:

Yasuo Shinohara, Tokushima University, Japan
Salvador Uribe-Carvajal, National Autonomous University of Mexico, Mexico
George A. Porter, Medical Center, University of Rochester, United States  

Copyright: © 2018 De Col, Petrussa, Casolo, Braidot, Lippe, Filippi, Peresson, Patui, Bertolini, Giorgio, Checchetto, Vianello, Bernardi and Zancani. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Prof. Marco Zancani, Università degli Studi di Udine, Udine, 33100, Italy, marco.zancani@uniud.it