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Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Physiol. | doi: 10.3389/fphys.2019.01079

Effects of Dietary-SCFA on Microbial Protein Synthesis and Urinal Urea-N Excretion Are Related to Microbiota Diversity in Rumen

Zhongyan Lu1, Hong Shen1 and  Zanming Shen1*
  • 1Nanjing Agricultural University, China

Two experiments were performed in this study. In Experiment 1, twenty goats were fed with an isonitrogenous diet, containing 28% Non-Fiber Carbohydrate (MNFC group, n=10) or 14% NFC (LNFC group, n=10). In the MNFC group, the ruminal concentration of SCFA increased, but pH declined. Compared with those in the LNFC group, the microbial protein synthesis in rumen and mRNA abundance of urea transporter B (UT-B) in rumen epithelium increased in the MNFC group, although serum urea-N (SUN) did not differ significantly between groups. Simultaneously, urinal urea-N excretion (UUE) was reduced in the MNFC group. Significant correlations were found between rumen SCFA and UT-B and between UT-B and UUE. Furthermore, the abundances of SCFA receptor of GPR41 and GPR43 increased in the rumen epithelium of the MNFC group. These results suggest that increases of SUN transported into the rumen and incorporated into MCP and decreases of UUE are related to ruminal SCFA. This is supported by data from our previous study in which added SCFA on the mucosal side caused increases of urea transport rate (flux Jsmurea) from the blood to the ruminal lumen side. In Experiment 2, we used 16S rRNA Amplicon Sequencing to analyze the structure of the ruminal microbiota community in relationship to SCFA. An additional eight goats were assigned into the MNFC (n=4) and LNFC (n=4) groups. The dietary ingredients, chemical composition, and feeding regimes were the same as those in Experiment 1. CCA analysis revealed NFC promoted the expansion of microbiota diversity, particularly of SCFA-producing microbes. The function prediction of 19 upregulated KEGG ortholog groups showed an NFC-induced increase of the types and abundances of genes coding for enzymes catalyzing N and fatty acid metabolism.
Based on our present and previous investigations, our results indicate that, in goats consuming an NFC-rich diet, the facilitated urea transport in the rumen and improved urea N salvage are triggered by an expansion of ruminal microbiota diversity and are signaled by ruminal SCFA. This study thus provides new insights into the microbiota involved in the dietary modulation of urea-N salvage in ruminant animals.

Keywords: Diet, Short chain fatty acid, Rumen, Urea transporter B, GPR41 and GPR43, microbial protein, microbiota, Urinal urea-N

Received: 08 Feb 2019; Accepted: 06 Aug 2019.

Copyright: © 2019 Lu, Shen and Shen. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Prof. Zanming Shen, Nanjing Agricultural University, Nanjing, 210095, Jiangsu Province, China, zmshen@njau.edu.cn