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Review ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Physiol. | doi: 10.3389/fphys.2019.01096

Principles of optogenetic methods and their application to cardiac experimental systems

Emily A. Ferenczi1, Xiaoqiu Tan2 and  Christopher L. Huang3, 4*
  • 1Department of Neurology, Massachusetts General Hospital, Harvard Medical School, United States
  • 2Key Laboratory of Medical Electrophysiology, Ministry of Education, Institute of Cardiovascular Medicine, Southwestern Medical University, China
  • 3Department of Physiology, Development and Neuroscience, Faculty of Biology, University of Cambridge, United Kingdom
  • 4Department of Biochemistry, University of Cambridge, United Kingdom

Optogenetic techniques permit studies of excitable tissue through genetically-expressed light-gated microbial channels or pumps permitting transmembrane ion movement. Light activation of these proteins modulates cellular excitability with millisecond precision. This review summarizes optogenetic approaches, using examples from neurobiological applications, then explores their application in cardiac electrophysiology. We review the available opsins, including depolarizing and hyperpolarizing variants, as well as modulators of G-protein coupled intracellular signaling. We discuss the biophysical properties that determine the ability of microbial opsins to evoke reliable, precise stimulation or silencing of electrophysiological activity. We also review spectrally-shifted variants offering possibilities for enhanced depth of tissue penetration, combinatorial stimulation for targeting different cell subpopulations, or all-optical read-in and read-out studies. Expression of the chosen optogenetic tool in the cardiac cell of interest then requires, at the single-cell level, introduction of opsin-encoding genes by viral transduction, or coupling “spark cells” to primary cardiomyocytes or a stem-cell derived counterpart. At the systems-level, this requires construction of transgenic mice expressing ChR2 in their cardiomyocytes, or in vivo injection (myocardial or systemic) of adenoviral expression systems. Light delivery, by laser or LED, with widespread or multipoint illumination, although relatively straightforward in vitro may be technically challenged by cardiac motion and light-scattering in biological tissue. Physiological read outs from cardiac optogenetic stimulation include single cell patch clamp recordings, multi-unit microarray recordings from cell monolayers or slices, and electrical recordings from isolated Langendorff perfused hearts. Optical readouts of specific cellular events, including ion transients, voltage changes or activity in biochemical signaling cascades, using small detecting molecules or genetically-encoded sensors now offer powerful opportunities for all-optical control and monitoring of cellular activity. Use of optogenetics has expanded in cardiac physiology, mainly using optically-controlled depolarizing ion channels to control heart rate and for optogenetic defibrillation. ChR2-expressing cardiomyocytes show normal baseline and active excitable membrane and Ca2+ signaling properties and are sensitive even to ~1 ms light pulses. They have been employed in studies of the intrinsic cardiac adrenergic system, and of cardiac arrhythmic properties.

Keywords: optogenetics, Cardiac Electrophysiology, opsin selection, Light delivery system, physiological readout, channelrhodopsin, halorhodopsin, archeorhodopsin, Melanopsin

Received: 10 Apr 2019; Accepted: 08 Aug 2019.

Copyright: © 2019 Ferenczi, Tan and Huang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Prof. Christopher L. Huang, Department of Physiology, Development and Neuroscience, Faculty of Biology, University of Cambridge, Cambridge, England, United Kingdom, clh11@cam.ac.uk