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Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Physiol. | doi: 10.3389/fphys.2019.01112

Circular RNA expression profiling and selection of key circular RNAs in the hypothalamus of heat-acclimated rats

 Wang Jing1, 2*,  Fan L. Jun2, 3, An G. Hong2,  Wang Shang2, Chen X. Wei2,  Liu Ying2, Ma Qiang2* and  Zhifeng Liu4*
  • 1Other, China
  • 2Tianjin Environmental Protection Science Research Institute, China
  • 3Tianjin University of Sport, China
  • 4Department of Critical Care Medicine, General Hospital of Guangzhou Military Command, China

Circular RNAs (circRNAs) play crucial roles in diverse biological processes. However, expression levels and functions of circRNAs related to heat acclimation (HA) are poorly understood. we, for the first time, used comprehensive circRNA expression profiling to investigate circRNA–miRNA interactions in HA rats, to provide a better understanding of mechanisms underlying HA. CircRNA expression profile was performed in rats’ hypothalamus of HA and control group with microarray assays. Bioinformatics analysis was conducted to predict their functions. And differential circRNAs and their regulated downstream miRNAs and mRNAs were quantitatively validated using real-time quantitative polymerase chain reaction (RT-qPCR ). Predicted proteins expression were determined using enzyme-linked immunosorbent assay (ELISA). In total, 53 circRNAs were differentially expressed between the HA and C group; 28 of these circRNAs were upregulated, while 25 were downregulated in HA (Fold change >1.5; P < 0.05). Three circRNAs and two miRNAs and three predicted mRNAs were obviously regulated after validated by RT-qPCR in HA rats. Two proteins expression were proportional to their mRNA changes. Further analysis indicated the circRNAs mostly close to HA were grouped into 3 signal pathways: including rno_circRNA_014301-vs-rno-miR-3575-vs-Hif-1α, rno_circRNA_014301-vs-rno-miR-3575-vs-Lppr4, rno_circRNA_010393-vs-rno-miR-20b-3p-vs-Mfap4 in hypoxia response pathways, substance/energy metabolism and inflammatory response pathways. Our findings implicate that many circRNAs regulate expressions of genes that interact with each other to exert their functions during HA.

Keywords: High-throughput RNA sequencing technology, circRNA, Heat acclimation, mRNA, microRNA

Received: 01 May 2019; Accepted: 12 Aug 2019.

Copyright: © 2019 Jing, Jun, Hong, Shang, Wei, Ying, Qiang and Liu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence:
Mrs. Wang Jing, Other, Tianjin, China, 18222309256@163.com
Mx. Ma Qiang, Tianjin Environmental Protection Science Research Institute, Nankai, China, maqiangw@sohu.com
Mx. Zhifeng Liu, Department of Critical Care Medicine, General Hospital of Guangzhou Military Command, Guangzhou, China, zhifengliu7797@163.com