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Original Research ARTICLE

Front. Physiol. | doi: 10.3389/fphys.2020.00268

Regulation of rest, rather than activity, underlies day-night activity differences in mice Provisionally accepted The final, formatted version of the article will be published soon. Notify me

  • 1Institut für Theoretische Biologie, Humboldt-Universität zu Berlin, Germany
  • 2Leiden University Medical Center, Netherlands

The suprachiasmatic nucleus (SCN), which serves as the central pacemaker in mammals, regulates the 24-hour rhythm in behavioral activity. However, it is currently unclear whether and how bouts of activity and rest are regulated within the 24-hour cycle (i.e., over ultradian time scales). Therefore, we used passive infrared sensors to measure temporal behavior in mice housed under either a light-dark (LD) cycle or continuous darkness (DD). We found that a probabilistic Markov model captures the ultradian changes in the behavioral state over a 24-hour cycle. In this model, the animal’s behavioral state in the next time interval is determined solely by the animal’s current behavioral state and by the “toss” of a proverbial “biased coin”. We found that the bias of this “coin” is regulated by light input and by the phase of the clock. Moreover, the bias of this “coin” for an animal is related to the average length of rest and activity bouts in that animal. In LD conditions, the average length of rest bouts was greater during the day compared to during the night, whereas the average length of activity bouts was greater during the night compared to during the day. Importantly, we also found that day-night changes in the rest bout lengths were significantly greater than day-night changes in the activity bout lengths. Finally, in DD conditions, the activity and rest bouts also differed between subjective night and subjective day, albeit to a lesser extent compared to LD conditions. The ultradian regulation represented by the model does not result in ultradian rhythms, although some weak ultradian rhythms are present in the data. The persistent differences in bout length over the circadian cycle following loss of the external LD cycle indicate that the central pacemaker plays a role in regulating rest and activity bouts on an ultradian time scale.

Keywords: spontaneous behavior, probabilistic model, Circadian clock, Activity duration, Light cycles

Received: 08 Nov 2019; Accepted: 09 Mar 2020.

Copyright: © 2020 Ananthasubramaniam and Meijer. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Dr. Bharath Ananthasubramaniam, Institut für Theoretische Biologie, Humboldt-Universität zu Berlin, Berlin, Germany, bharath.ananth@charite.de