Bo Zhang
Joseph D. Powers
Andrew D. McCulloch
Neil C. Chi1,2,3,4*- 1Department of Bioengineering, University of California San Diego, La Jolla, CA, United States
- 2Institute for Engineering in Medicine, University of California San Diego, La Jolla, CA, United States
- 3Department of Medicine, Division of Cardiovascular Medicine, University of California San Diego, La Jolla, CA, United States
- 4Institute of Genomic Medicine, University of California San Diego, La Jolla, CA, United States
Mechanosignaling describes processes by which biomechanical stimuli are transduced into cellular responses. External biophysical forces can be transmitted via structural protein networks that span from the cellular membrane to the cytoskeleton and the nucleus, where they can regulate gene expression through a series of biomechanical and/or biochemical mechanosensitive mechanisms, including chromatin remodeling, translocation of transcriptional regulators, and epigenetic factors. Striated muscle cells, including cardiac and skeletal muscle myocytes, utilize these nuclear mechanosignaling mechanisms to respond to changes in their intracellular and extracellular mechanical environment and mediate gene expression and cell remodeling. In this brief review, we highlight and discuss recent experimental work focused on the pathway of biomechanical stimulus propagation at the nucleus-cytoskeleton interface of striated muscles, and the mechanisms by which these pathways regulate gene regulation, muscle structure, and function. Furthermore, we discuss nuclear protein mutations that affect mechanosignaling function in human and animal models of cardiomyopathy. Furthermore, current open questions and future challenges in investigating striated muscle nuclear mechanosignaling are further discussed.
1 Introduction
Striated muscles, including cardiac muscle, skeletal muscle, or insect flight muscles, are highly organized tissues specifically designed to produce precise forces and movements for a diverse set of functions. Striated muscle cells (myocytes) comprising these tissues all share the remarkable ability to both produce and adapt to highly variable microenvironmental forces. During myocyte contraction, ATP-driven myosin motors on the thick filament of the sarcomere, the contractile unit of myocytes, cyclically interact with actin filaments to induce filament sliding and force-generating sarcomere shortening (Powers et al., 2021). For these molecular-level processes to translate to cell- and tissue-level force production, sarcomere-generated forces must be transmitted from the sarcomere, to the intermediate filaments of the cytoskeleton and costamere, and outward to the extracellular matrix (ECM) and surrounding cells. Similarly, forces generated from outside the cell (e.g., passive stretch, ECM stiffness) are transmitted into and distributed throughout the cell, putatively through the same molecular structures.
Both intracellular and extracellular forces ultimately reach the myocyte nucleus, where they can be processed into biological responses that mediate the structural or functional adaptation of the cell through a process called nuclear mechanosignaling. The nucleus of striated muscle cells, among many other cell types (Maurer and Lammerding, 2019), is now considered to be a key mechanosensor, reacting to forces at the nucleus-cytoskeleton interface and mediating downstream biological responses. Factors such as nuclear envelope structure, intracellular nuclear positioning, and nuclear deformation can influence the mechanosensing ability and response to mechanical forces (Jabre et al., 2021). Therefore, myocytes and their nuclei must maintain a mechanical homeostasis during muscle contractions and deformations to preserve healthy muscle structure and function.
However, this homeostasis can be disrupted by defects in the ability of the myocyte nucleus to sense the mechanical forces and/or by abnormal mechanical forces imposed onto the nucleus. Myocytes under persistent abnormal mechanical forces, such as chronically altered contractility or abnormal strains imposed during passive muscle stretch, can trigger a myriad of biological responses that often lead to maladaptive cell remodeling or hypertrophy, contractile dysfunction, and cell death (Dupont et al., 2011; Kresh and Chopra, 2011). In mammalian hearts, for example, prolonged high load or deposition of extracellular matrix (ECM) can lead to cardiac hypertrophy, cardiomyopathy, and heart failure (Cook et al., 2014; Lyon et al., 2015; Yadav et al., 2021). Moreover, skeletal muscle cells with defective proteins that mediate the structural integrity of the nucleoskeleton or the ability of the nucleus to sense mechanical loading can lead to muscular dystrophy phenotypes (Bianchi et al., 2018).
Elucidating the role of nuclear mechanosignaling in mechanosensitive genetic signaling pathways and their involvement in muscular diseases is currently a major research area, but there are still many unknown mechanisms about the transduction of intracellular and extracellular forces into downstream gene expression via nuclear mechanosignaling. Here, we briefly review our current understanding of mechanisms by which mechanical forces and nuclear mechanosensing influence gene regulatory programs underlying pathological remodeling and dysfunction of striated muscle cells.
2 The bridge between chromatin and the extracellular matrix of striated muscle cells
In healthy striated muscle cells, the pseudo-crystalline structure of myofilaments, sarcomeres, and myofibrils establishes a highly organized and uniquely anisotropic subcellular architecture. This subcellular organization is, in part, maintained by rapid and spatially controlled protein turnover (Wood et al., 2022) of large macromolecular complexes, which together provide mechanical linkages between chromatin, the cytoskeletal and sarcomere network, and the extracellular matrix (Figure 1). At the cell membrane-ECM interface, the cell connection to the ECM is supported by transmembrane protein complexes called costameres. As depicted in Figure 1, the costamere is a molecular complex that consists of integrin, vinculin, integrin-linked kinase, ankyrin, filamin C, talin, desmin, dystrophin, the dystrophin-glycoprotein complex (DGC), among many others (Bang et al., 2022). The costamere also helps connect the Z-disk of the sarcomere to the sarcolemma and the ECM, providing a structural and mechanical link between the intracellular contractile machinery and the extracellular environment. Deeper into the cell interior, a framework of microtubules, intermediate filaments, and F-actin maintain the connection of the Z-disks throughout the cytoskeleton and to the nucleoskeleton.
FIGURE 1. Schematic of the intracellular environment of a mature cardiomyocyte, illustrating mechanical coupling between the ECM the costamere, the sarcomere, the nucleoskeleton, and chromatin. Costameres consists of integrins, laminins, and DGCs (among other proteins not depicted here) and provides linkages between the Z-disks of the sarcomere, IFs in the cortical cytoskeleton, and the ECM, and other cytoskeletal IFs. Desmin, a-actinin, and Filamin C provide (among other proteins not shown here for clarity) provide structural integrity to the Z-disk, to which myofilaments of the sarcomere are anchored. ECM, extracellular matrix; IFs, intermediate filaments; DGC, dystrophin-glycoprotein complex; LAP1/2, lamina-associated polypeptide 1/2; NPC, nuclear pore complex; NE, nuclear envelope.
The correct crosstalk between cytoskeleton and nucleoskeleton is necessary for proper mechanosignaling in straited muscle cells. Muscle-specific LIM domain protein (MLP) has been shown to be a stretch sensor in cardiac muscle in both mice and human (Knöll et al., 2002). MLP KO mice showed severe DCM phenotype and mRNA profile. Cardiomyocytes from these mice indicate significant disruption in their cardiomyocyte cytoarchitecture and myofibril arrays (Arber et al., 1997). It is thought that MLP-TCAP binding is necessary for proper cardiac muscle mechanotransduction. Loss of MLP-TCAP changes intrinsic elastic properties of titin, which further affects proper mechanotransduction from the ECM to the nucleus (Arber et al., 1997; Knöll et al., 2002). Another example of cytoskeleton-nucleoskeleton crosstalk is desmin [please see (Capetanaki et al., 2015; Hnia et al., 2015; Tsikitis et al., 2018) for more comprehensive reviews on desmin in muscle diseases]. Desmin interfilament cytoskeleton network connects with the lamin nucleoskeleton either directly through the nuclear pores or indirectly through plectin-nesprin3-LINC complex (Georgatos et al., 1987; Lockard and Bloom, 1993; Frock et al., 2006). This crosstalk is essential for propagating mechanical stimuli from ECM to the nucleus. Depletion of either desmin or Nesprin-3 causes nuclear envelope deformation and chromatin disorganization (also discussed in Section 4, below). Moreover, desmin-KD cardiomyocytes showed an altered expression profile that has significant cardiac dysfunction, DCM, hypertrophy signatures (Heffler et al., 2020). Patients with dysfunctional desmin mutations showed granulofilamentous accumulations and sandwich formations and characteristic z-disk deformity (Vrabie et al., 2005; Claeys et al., 2008). These abnormal structures might affect cytoskeleton and nucleoskeleton crosstalk. Furthermore, in addition to nuclear mechanical regulation, desmin also serves as a signaling platform to help its binding partners in significant cellular functions like DNA repair (with MLH1), endocytosis pathway (with ITSN1), and calcium hemostasis (with S100A1) (Hnia et al., 2015).
The primary connection between the nucleus and cytoskeleton in striated muscles is a protein complex called the Linker of Nucleoskeleton and Cytoskeleton (LINC) complex, which comprise many proteins that have been associated with cardiomyopathies, demonstrating its critical role in maintaining striated muscle function [please see (Stroud et al., 2014; Stroud, 2018; Bang et al., 2022) for reviews on this subject]. As illustrated in Figure 1, the LINC complex is part of the molecular connections between the nuclear lamina and the cytoskeletal/sarcomeric network, which is facilitated (in part) by nesprins at the outer nuclear membrane and SUN domain proteins (SUN1/2) at the inner nuclear membrane. In the inner nucleus, chromatin interacts directly with the LINC complex via emerin, lamins, and many other proteins. In particular, the interaction between lamin and desmin in cardiomyocytes is critical for normal heart function. A lack of lamin A/C expression in mice cardiomyocytes has been shown to exhibit disorganized desmin filaments and paranuclear regions where the desmin filaments are detached from the nucleus (Nikolova et al., 2004). Many Lamin A/C mutations also show different degrees of disruption on cytoskeleton structures in both human and mice models (Sebillon et al., 2003; Mounkes et al., 2005; Lanzicher et al., 2015; Yang et al., 2021). In a mouse model of dilated cardiomyopathy caused by the lamin A/C mutation H222P, desmin loses its localization from Z-disks and intercalated disks and forms aggregates (Galata et al., 2018). Interestingly, ameliorating the desmin-associated defects in these cardiomyocytes provided cardioprotection from the H222P lamin A/C mutation (Galata et al., 2018), suggesting that desmin is a promising target for certain laminopathies.
Thus, myocytes, like many other cell types, exhibit tight mechanical coupling from chromatin to ECM. However, the anisotropic subcellular ultrastructure of myocytes and their high degree of force production impose distinct mechanical environments onto myonuclei that may elicit unique and underexplored nuclear mechanosignaling pathways underlying muscle structure and function. In the sections that follow, we discuss specific proteins known to be involved in the maintenance of this process, and the relationship between defects in nuclear mechanosignaling, mechanosensitive gene regulation and protein synthesis, and striated muscle diseases.
3 Functions of nuclear envelope and nucleoskeleton proteins in healthy and diseased myocytes
The nuclear envelope (NE) and nucleoskeleton provide a connection between the sarcomere, the cytoskeleton, and chromatin in myocytes, which likely facilitates important mechanosensitive gene regulatory programs. In the following subsections, we discuss specific proteins known to be involved in nuclear mechanosignaling or nucleus-related pathologies in striated muscle.
3.1 Nesprins
Nesprins provide many of the linkages between the outer nuclear membrane and cytoskeletal proteins. Nesprins generally are composed of three domains: a highly conserved C-terminus which interacts with the SUN (Sad1p, UNC-84) protein at the outer nuclear membrane (ONM), a spectrin repeat domain, and a diverse N-terminus that binds to cytoskeleton structures (Stroud, 2018; Liao et al., 2019). Nesprin-1/2 can also directly bind to actin filaments (Chancellor et al., 2010; Zhou et al., 2018), while nesprin-1α2 indirectly interacts with microtubule structures through Kinesin 1 (Mislow et al., 2002; Briand and Collas, 2020). Nesprin-3 indirectly binds to intermediate filaments through plectin (Wilhelmsen et al., 2005), and microtubules through Microtubule-Actin Cross-linking Factor (MACF) and Bullous pemphigoid antigen 1 (BPAG1) (Ketema and Sonnenberg, 2011; Liao et al., 2019). The interaction between Nesprin and SUN forms the basic structure of LINC that can propagate forces between the cytoskeleton and the nucleus.
Nesprin-1/2 mutations have been found to be correlated with Emery-Dreifuss muscular dystrophy (EDMD) and dilated cardiomyopathy (DCM) (Zhang et al., 2007; Puckelwartz et al., 2010). One patient with mutation in the C-terminus of nesprin-1 (p.R374H) experiences DCM with elevated expression of nesprin-1 and lamin A/C (Puckelwartz et al., 2010). Mice that have C-terminus KASH-domain deleted in nesprin-1 developed EDMD-like and DCM phenotypes. Gene-edited mice where the C-terminus of nesprin-1 was deleted experience hindlimb muscle weakness and cardiac conduction defects (Puckelwartz et al., 2009). Nesprin-1/2 siRNA knockdown in fibroblasts leads to abnormal nuclear morphology and mislocalization of emerin and SUN-2 (Zhang et al., 2007). Surprisingly, nesprin-3 mutation, deletion, or overexpression in mice and zebrafish does not seem to cause significant phenotypes (Banerjee et al., 2014).
3.2 SUN proteins
SUN1/2 are expressed in skeletal and cardiac muscles in humans (Crisp et al., 2006; Puckelwartz et al., 2009; Zhang et al., 2010). They both have a C-terminal SUN domain, a coiled-coil domain, a transmembrane (TM) domain, and an N-terminus nucleoplasmic domain (Stroud, 2018; Sosa Ponce et al., 2020). The SUN domain is the most conserved domain that interacts with the KASH domain on Nesprin (Sosa et al., 2012; Wang et al., 2012; Sosa Ponce et al., 2020). Inter-protein interactions between the coiled-coil domains form the SUN protein trimer (Sosa et al., 2012; Jahed et al., 2018a; Jahed et al., 2018b; Sosa Ponce et al., 2020). The N-terminus nucleoplasm domain binds with emerin and lamin A/C, anchoring the SUN protein at the NE (Crisp et al., 2006; Haque et al., 2010).
SUN1/2 mutations are rarely associated with cardiomyopathy (Meinke et al., 2014; Bang et al., 2022). However, patients carrying SUN mutations can experience significant cardiac abnormalities when there are also mutations in other LINC proteins (Manila et al., 1998; Bonne et al., 2000; Vytopil et al., 2003). Neither global knockout (KO) of SUN1/2 in mice show significant defects in heart or skeletal muscle (Ding et al., 2007; Lei et al., 2009). However, double-KO of SUN1/2 in mice leads to premature death due to central neuron system defects (Lei et al., 2009). Nesprin-1 but not lamin A/C are mislocalized in SUN1/2 double-KO mice skeletal muscle cells (Lei et al., 2009). These results indicate that SUN1 and SUN2 play a critical but potentially redundant role in Nesprin-1 localization on skeletal muscle NE.
3.3 Emerin
Emerin has a long N-terminus in the nucleoplasmic domain that interacts with SUN1/2, LAP1, lamin A/C, histone deacetylase 3 (HDAC3), and barrier to autointegration factor (BAF) (Lee et al., 2001; Demmerle et al., 2012; Shin et al., 2013; Berk et al., 2014). HDAC3 and BAF help emerin indirectly bind with chromatin, and the lamin A/C and LAP1 interaction with emerin is thought to be important for its localization at inner nuclear membrane (INM) (Mislow et al., 2002; Haque et al., 2010; Guilluy et al., 2014; Shin et al., 2014; Osmanagic-Myers et al., 2015).
Mutations in emerin are clinically observed to be related to EDMD, DCM, and other cardiac and skeletal muscle defects (Bione et al., 1994; Bione et al., 1995; Essawy et al., 2019). Emerin KO mice were recently developed to study the effect of loss of emerin (Lammerding et al., 2005; Melcon et al., 2006). Surprisingly, emerin KO mice show no significant difference in skeletal muscle development and only a mild reduction in ejection fraction (Melcon et al., 2006; Ozawa et al., 2006; Stubenvoll et al., 2015). However, LAP1 and emerin double-KO mice showed exacerbated myopathy, suggesting that LAP1 and emerin may have compensatory functions in myocytes (Shin et al., 2013).
3.4 Lamina-associated polypeptides (LAP proteins)
Lamina-associated polypeptide 1 (LAP1) is encoded by the gene TOR1AIP1. In humans, two isoforms of LAP1 (LAP1B and LAP1C) are expressed through alternative splicing (Kondo et al., 2002; Santos et al., 2014). Both LAP1 isoforms locates at the INM and LAP1B has an N-terminus nucleoplasmic domain that interacts with Emerin, lamin A/C, lamin B1, and chromatin (Foisner and Gerace, 1993; Shin et al., 2013; Luithle et al., 2020). It also has a C-terminus that interacts with Torsin A, B, 2, and 3 (Goodchild and Dauer, 2005; Jungwirth et al., 2010; Kim et al., 2010). Because of its close interaction with mechanosignaling proteins at the INM, LAP1 is suspected to be involved in the nuclear mechanosensing (Shin et al., 2013). However, the exact mechanism of LAP1 specific mechanosignaling remains unknown.
Patients with LAP1 mutation p.Arg321* showed significant decrease in expression of both LAP1 isoforms in mRNA and Protein level (Fichtman et al., 2019). This specific mutation truncates the entire transmembrane domain and cytoplasmic domain. Skin fibroblast from patients showed distorted nuclear shape and abnormal lamin A/C localizations. Patients also suffer from congenital heart malformations and a series of other abnormalities. Another frameshift LAP1 mutation (c.186delG) leads to an early stop codon (Kayman-Kurekci et al., 2014). The consanguineous patients carrying this mutation showed muscle weakness and atrophy, rigid spine, and rigid interphalangeal hand joints. Only one patient showed decreased cardiac function. Interestingly, this mutation only exists at the LAP1B isoform while LAP1C is unaffected. Interestingly, patients’ skeletal muscle biopsy showed significantly reduced LAP1B and slightly increased LAP1C expression. The biopsy also showed intact sarcomere organization but fragmented nuclear membrane, nuclear blebbing, and naked chromatin. Because of the relatively mild phenotypes in this isoform specific mutation, this study suggests that LAP1C might be complementary to LAP1B function and LAP1B is important for nuclear structure maintenance (Kayman-Kurekci et al., 2014). Furthermore, mice with conditional LAP1 KO in striated muscle cells experienced significant cardiac and skeletal myopathy (Shin et al., 2013). Skin fibroblasts from global LAP1-KO mice showed co-mislocalization of emerin and lamin A/C, indicating the potential role of LAP1 in localizing LINC proteins.
LAP2 has six isoforms derived from alternative splicing. All of the six isoforms have an N-terminus with a LEM (LAP2, emerin, MAN1) domain that interacts with BAF that further binds with chromatin (Lin et al., 2000). The C-terminus of each LAP2 protein is highly variable in length and possesses different functions. For example, LAP2α has the longest C-terminus domain which lacks a TM domain but interacts with lamin A/C, while LAP2β interacts with lamin B1 at the INM and possesses a TM domain (Foisner and Gerace, 1993; Dechat et al., 1998). For more details about isoform domains and protein interactions, we recommend the following review (Dechat et al., 2000a).
Patients with LAP2α p.R690C mutation have been reported with severe DCM (Taylor et al., 2005). This mutation occurs within the domain where LAP2α interacts with lamin A/C, and in vitro functional analysis shows interaction of mutated LAP2α with the pre-lamin A is significantly compromised compared to the wild-type LAP2α. Interaction of lamin A/C and LAP2α seems to be the common mechanism for LAP2α mutations that lead to diseases in other systems as well (Pekovic et al., 2007; Gesson et al., 2014; Vidak et al., 2018).
3.5 Nuclear pore complex (NPC)
The nuclear pore complex (NPC) mediates the transport of all macromolecules between the nucleus and the sarcoplasm. NPC is composed of ∼30 different proteins with multiple copies of each nucleoporin (Cronshaw et al., 2002), and these nucleoporin constitute three different regions: the cytoplasmic ring, the central framework, and the nuclear basket (Hurt and Beck, 2015). NUP153 is a nucleoporin on the nuclear basket that interacts with SUN1, lamin A/C and lamin B (Liu et al., 2007; Al-Haboubi et al., 2011; Li and Noegel, 2015). It is suspected that NPCs possess mechano-activation through the NUP153-SUN1 interaction. It is hypothesized that force propagation from SUN1 to NUP153 can induce rearrangement of the basket structure that extends from the NPC into the nucleus (Donnaloja et al., 2019). Depletion of NUP153, which alters the localization of Sun1 and F-actin structure, further supports this hypothesis (Zhou and Panté, 2010).
Mutations on NUP153 are not directly correlated with skeletal or cardiac myopathy according to our knowledge. However, skin fibroblasts with lamin A/C mutations within the Ig-fold domain, which interact with NUP153 shows altered NUP153 localization (LMNA p.453W) (Al-Haboubi et al., 2011). Another mutation on lamin A/C (p.A399C) also disrupts the lamin A/C-NUP155 interaction and causes atrial fibrillation (Han et al., 2019). Interestingly, ischemic and dilated cardiomyopathy hearts harvested from patients showed significant increase in NUP153 (Tarazón et al., 2012), however the mechanism behind NUP153 up-regulation and the downstream effects in these patients is not clear.
4 Nuclear morphology, migration, and deformation in striated muscle diseases
It is clear that nuclear morphology and deformation play an important role in regulating gene expression in many different cell types (Thomas et al., 2002; Kalukula et al., 2022). For example, extracellular-based mechanical tension across the nuclear membrane of aortic valve fibroblasts, mediated by the actin cytoskeleton and LINC complex, facilitates chromatin remodeling that leads to pro-fibrotic myofibroblast persistence (Walker et al., 2021). In striated muscle cells, the large magnitude of myocyte deformations experienced during muscle contraction and relaxation may facilitate processes involving nuclear migration, chromatin organization, and mechanosensitive gene expression. For example, during skeletal muscle contractions, centripetal forces exerted by myofibrils facilitate the migration of the nucleus to the periphery of the myofibers (Roman et al., 2017). Furthermore, myonuclei are readily localized via cytoskeletal protein networks to sites of myocyte damage to accelerate sarcomere repair (Roman et al., 2021). Moreover, physiological myonuclear deformations occur during normal contractions and passive stretches (Kalukula et al., 2022). A recent study (Ghosh et al., 2019) demonstrated that intranuclear deformations during cardiomyocyte contraction depends on the mechanical properties of the extracellular environment, and that areas in the nucleus with denser chromatin exhibited greater strain discrepancies between substrate stiffnesses. This supports the hypothesis that myonuclei transduce the mechanical properties of the extracellular environment into epigenetic control via intranuclear strain distributions that affect chromatin organization and accessibility.
More compliant and fragile nuclei, as well as decoupled nucleus-cytoskeleton connections, can be consequences of nuclear envelope protein mutations associated with various myopathies (Zwerger et al., 2013). In Drosophila larvae with mutated Nesprin, myonuclei deform differently during contractions compared with wild-type flies (Lorber et al., 2020). In an aging context, cardiomyocytes from Drosophila also exhibit decreased Lamin C expression with age, which coincides with decreased nuclear size, increased nuclear stiffness, and altered transcriptional programs (Kirkland et al., 2023). Similarly, aged mice and non-human primates exhibit reduced nuclear size and circularity, with old mice also having reduced Lamin C compared to younger mice (Kirkland et al., 2023). Moreover, mutant lamins in skeletal muscle associated with laminopathies and muscular dystrophy cause nuclear envelope rupture and DNA damage that correlate with disease severity in both mice and humans (Earle et al., 2020). However, microtubule stabilization reinforced myonuclei in these diseased muscle fibers, reducing nuclear damage and rescued myocyte function in viability in cells with mutated lamins (Earle et al., 2020).
Defects or mutations in proteins comprising the nucleoskeleton are not only associated with nuclear envelopathies and laminopathies, but they can also lead to altered nuclear morphology and mechanotransduction (Janin and Gache, 2018; Ross and Stroud, 2021). Mice lacking the nuclear envelope transmembrane protein 39 (Net39) develop skeletal muscle dysfunction and remodeling that resembles Emery Dreifuss muscular dystrophy (Ramirez-Martinez et al., 2021). Moreover, the nuclei in skeletal muscle fibers from Net39-null mice exhibit significant deformations, changes in chromatin accessibility, and altered gene expression, while human muscle biopsies from patients with Emery Dreifuss muscular dystrophy revealed a downregulation of Net39 (Ramirez-Martinez et al., 2021). [See (Bianchi et al., 2018) for a recent review of Emery Dreifuss muscular dystrophy in the context of nuclear mechanosignaling.]
In the heart, a loss of desmin or nesprin in the LINC complex in cardiomyocytes causes the nucleus to collapse in response to neighboring polymerizing microtubules, which can lead to chromatin reorganization, DNA damage, and altered transcriptomic expression (Heffler et al., 2020). Similarly, genetically engineered mice carrying the human p.L13 > R mutation in the nuclear envelope protein LEM domain containing protein 2 (LEMD2) develop severe DCM and cardiac fibrosis, which lead to premature death (Caravia et al., 2022). Homozygous mice for the p.L13 > R LEMD2 variant exhibit disorganized heterochromatin, nuclear envelope deformations, extensive DNA damage, and apoptosis linked to p53 activation (Caravia et al., 2022), suggesting that LEMD2 is essential for genome stability and cardiac function.
Finally, we note that defects in cytoskeletal proteins not directly linked to the nuclear envelope (but associated with other cardiomyopathies) also can cause changes in nuclear structure. For example, a loss of filamin C in adult mouse hearts causes nuclear remodeling and altered gene expression (Zhou et al., 2020; Powers et al., 2022; Powers and McCulloch, 2022), suggesting that cytoskeletal proteins not directly interacting with the nucleoskeleton in myocytes may also influence nuclear structure and mechanosignaling.
5 Mechanical regulation of chromatin positioning, epigenetic factors, and gene expression in myocytes
In the nucleus, chromatin accessibility and its three-dimensional organization are correlated (Bertero et al., 2019; Bertero and Rosa-Garrido, 2021). As such, mechanical forces and/or defects in the nucleoskeleton structure that affect chromatin organization may be key factors underlying mechanosensitive gene regulation of pathogenic remodeling or dysfunction in myocytes. Lamin has been shown to organize the three-dimensional genome from the nuclear periphery (Zheng et al., 2018). While the conventional view is that chromatin activity is reduced from the nuclear center to periphery, a recent study (Amiad-Pavlov et al., 2021) found that A-type lamin upregulation in instar larva muscle caused chromatin to collapse toward the nuclear center while also reducing overall levels of active chromatin. These findings suggest that: 1) lamina composition regulates the organization of peripheral chromatin, and 2) there may not be a clear spatial distribution of active versus inactive chromatin throughout the nuclear volume. Furthermore, a loss of all isoforms of lamin has been shown to expand or detach specific lamina-associated domains (LADs), and dysregulate the active and repressive chromatin domains among different topologically associated chromatin domains (TADs) (Zheng et al., 2018). Lamin mutations also cause increased Yes-associated protein (YAP) nuclear entry in muscle stem cells (Owens et al., 2020) and alter chromatin interactions and depress alternative fate genes (Shah et al., 2021). With the emerging new findings on lamin A/Polycomb Repressive Complex (PRC) crosstalk (Marullo et al., 2016), it is speculated lamin A can also affect gene expression through histone modifications (Bianchi et al., 2018). PRC proteins are known to form PRC aggregates that form an intranuclear structure that regulates long-range chromatin interactions (Lanzuolo et al., 2007; Bantignies et al., 2011; Cesarini et al., 2015). Polycomb Repressive Complex 2 (PRC2) is responsible for H3K27me3 through the catalytic activity of Ezh2 (Bianchi et al., 2018). It is speculated that, under mechanical stress, lamin A is upregulated, leading to more crosstalk with PCR2, resulting in observed accumulation of H3K27me3 (Bianchi et al., 2018). This hypothesis also explains the higher mechanosignaling sensitivity observed in cells carrying lamin A/C mutation: mutated lamin A/C cannot achieve effective crosstalk with PRC2 which further leads to a loss of repressive activity on mechanosensitive genes.
Mechanical stimulation also affects nuclear translocation of transcription factors. Under mechanical stimulation, myocardin-related transcription factors (MRTFs), YAP, and extracellular signal-regulated kinase (ERK) are translocated into the nucleus in myocytes (Wada et al., 2011; Aragona et al., 2013; Velasquez et al., 2013; Gallo et al., 2019). Two mechanisms of NPC mediated nuclear translocation have been proposed: the first hypothesis is that the NPC might increase the ring structure diameter under the mechanical force because of its connection with SUN2 protein. The enlargement of the NPC tunnel promotes the entry of transcriptional factors. The second hypothesis is that the mechanical stimulation changes the state of transcriptional factors which subsequently increase their binding affinities with nuclear transport receptors (Matsuda and Mofrad, 2022). As cells show reduced mechanosensitivity with dysfunction of NPC, both hypotheses could be correct (Mofrad and Kamm, 2009; Fichtman and Harel, 2014).
Emerin, LAP2, and MAN1 proteins are all referred to as LEM proteins. All of them have a LEM domain where they interact with chromatin indirectly via BAF (Brachner and Foisner, 2011), which helps facilitate the tethering of chromatin around the nuclear periphery. Patients with EDMD show less repressed chromatin and altered chromatin structure in their skeletal muscle (Ognibene et al., 1999; Fidziańska and Hausmanowa-Petrusewicz, 2003; Mewborn et al., 2010). Under force, emerin monomers are phosphorylated, unbind from BAF, and form oligomers which strengthens their interaction with SUN protein and lamin A/C (Guilluy et al., 2014; Fernandez et al., 2022). Through this mechanism, emerin alters the chromatin organization under force. All of the six LAP2 isoforms can bind with BAF and affect chromatin organization (Wilson and Foisner, 2010). Other than its BAF interactions, LAP2 can also affect chromatin organization through interaction with other nuclear proteins. While other isoforms primarily affect chromatin LAD distribution through interacting with B type lamins, LAP2α only binds with lamin A/C (Dechat et al., 2000b; Naetar et al., 2008). Furthermore, LAP2α also interacts with chromatin-binding behavior of the high-mobility group N protein 5 (HMGN5), and downregulation of LAP2α leads to disorganized HMGN5-targeted chromatin regions (Zhang et al., 2013). In addition to interacting with BAF and lamins, MAN1 can also repress TGFβ, activin, and BMP signaling through its interaction with Smad proteins (Pan et al., 2005). However, this interaction is not myocyte or cardiomyocyte specific. In fact, MAN1’s function in mechanosignaling and in myocytes is understudied. It will be interesting to investigate whether MAN1 can interact with Smad-like proteins in myocytes and to subsequently map out the resulting gene regulatory network.
6 Current open questions and remaining challenges
There is a growing body of research that we were not able to include in this review. Nuclear mechanosignaling has been a rapidly developing field of research for decades, and it provides an exciting direction in investigating muscle and heart adaptation, maturation, and disease models. As the majority of the groundwork for understanding the role of nuclear mechanosignaling in cell state and function has been laid in non-muscle cell types, there is now an exciting opportunity to apply this knowledge to investigations into mammalian myocytes. Doing so will reveal novel mechanisms of the mechanical regulation of myocyte structure and function that will be critical in defining disease mechanisms and investigating new therapies for myopathies. Considering the well-established gene editing protocols to edit stem cell-derived myocytes, verifying mechano-sensing protein function and mechanism in human cells is a particularly promising endeavor for future and ongoing research. Moreover, much of our understanding of nucleoskeleton protein function is focused on well-studied proteins like lamins and emerin, while the functions of understudied proteins (such as MAN1) remain far from understood. As such, new research on the variety of other nuclear proteins and their role in regulating nuclear structure, chromatin organization, and nucleoskeletal mechanics will construct a more complete picture of the mechanosignaling processes of healthy and diseased myocytes.
Building upon the rich history of muscle research in which multidisciplinary approaches are embedded, combining multiple existing methodologies and technologies to answer new and exciting questions about myonuclear mechanosignaling will be undoubtedly advantageous. For example, bioinformatics (i.e., quantitative proteomics and transcriptomics) can be applied to future studies to gather a more complete picture of effects of mechanical stimulation and protein mutations in striated muscle mechano-signaling processes. These types of studies will also inform computational investigations into mechanosensitive genetic signaling in myocyte remodeling (Tan et al., 2017; Saucerman et al., 2019; Khalilimeybodi et al., 2023), which will enable a more holistic view of nuclear mechanosensing and myocyte structure/function. Furthermore, studying cells/tissues on a stationary surface/scaffold does not accurately represent the in vivo environment of muscles where the cells are constantly experiencing large forces and deformations. Mechanically stimulating the cells to better mimic the innate physical environment can provide more accurate representations of the mechano-sensing protein functions. Exciting ongoing research in these directions is sure to have a great impact on our understanding of the role of the nucleus in mediating muscle function in health and disease.
Author contributions
BZ and JP wrote the manuscript together. JP made the figure used in the manuscript. AM and NC gave instructions and guidance in editing the manuscript.
Funding
JP was supported by the National Heart, Lung, and Blood Institute grants K99 HL159224. The cartoon illustration in this paper was made using BioRender. This work was supported in part by grants from the NIH to NC.
Conflict of interest
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Publisher’s note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.
References
Al-Haboubi, T., Shumaker, D. K., Koser, J., Wehnert, M., and Fahrenkrog, B. (2011). ‘Distinct association of the nuclear pore protein Nup153 with A- and B-type lamins’. Nucleus 2 (5), 500–509. doi:10.4161/nucl.2.5.17913
Amiad-Pavlov, D., Lorber, D., Bajpai, G., Reuveny, A., Roncato, F., Alon, R., et al. (2021). Live imaging of chromatin distribution reveals novel principles of nuclear architecture and chromatin compartmentalization. Sci. Adv. 7, eabf6251. doi:10.1126/sciadv.abf6251
Aragona, M., Panciera, T., Manfrin, A., Giulitti, S., Michielin, F., Elvassore, N., et al. (2013). ‘A mechanical checkpoint controls multicellular growth through YAP/TAZ regulation by actin-processing factors’. Cell. 154 (5), 1047–1059. doi:10.1016/j.cell.2013.07.042
Arber, S., Hunter, J. J., Ross, J., Hongo, M., Sansig, G., Borg, J., et al. (1997). ‘MLP-Deficient mice exhibit a disruption of cardiac cytoarchitectural organization, dilated cardiomyopathy, and heart failure’. Cell. 88 (3), 393–403. doi:10.1016/s0092-8674(00)81878-4
Banerjee, I., Zhang, J., Moore-Morris, T., Pfeiffer, E., Buchholz, K. S., Liu, A., et al. (2014). ‘Targeted ablation of nesprin 1 and nesprin 2 from murine myocardium results in cardiomyopathy, altered nuclear morphology and inhibition of the biomechanical gene response’. PLoS Genet. 10, e1004114. doi:10.1371/journal.pgen.1004114
Bang, M., Bogomolovas, J., and Chen, J. (2022). Understanding the molecular basis of cardiomyopathy. Am. J. Physiol. Circ. Physiol. 322, H181–H233. doi:10.1152/ajpheart.00562.2021
Bantignies, F., Roure, V., Comet, I., Leblanc, B., Schuettengruber, B., Bonnet, J., et al. (2011). ‘Polycomb-Dependent regulatory contacts between distant hox loci in Drosophila’. Cell. 144 (2), 214–226. doi:10.1016/j.cell.2010.12.026
Berk, J. M., Simon, D. N., Jenkins-Houk, C. R., Westerbeck, J. W., Gronning-Wang, L. M., Carlson, C. R., et al. (2014). ‘The molecular basis of emerin–emerin and emerin–BAF interactions’. J. Cell. Sci. 127 (18), 3956–3969. doi:10.1242/jcs.148247
Bertero, A., Fields, P. A., Ramani, V., Bonora, G., Yardimci, G. G., Reinecke, H., et al. (2019). Dynamics of genome reorganization during human cardiogenesis reveal an RBM20-dependent splicing factory. Nat. Commun. 10, 1538. doi:10.1038/s41467-019-09483-5
Bertero, A., and Rosa-Garrido, M. (2021). Three-dimensional chromatin organization in cardiac development and disease. J. Mol. Cell. Cardiol. 151, 89–105. doi:10.1016/j.yjmcc.2020.11.008
Bianchi, A., Manti, P. G., Lucini, F., and Lanzuolo, C. (2018). ‘Mechanotransduction, nuclear architecture and epigenetics in Emery Dreifuss muscular dystrophy: Tous pour un, un pour tous’. Nucleus 9 (1), 276–290. doi:10.1080/19491034.2018.1460044
Bione, S., MaEstrini, E., Rivella, S., Mancini, M., RegiS, S., Romeo, G., et al. (1994). ‘Identification of a novel X-linked gene responsible for Emery-Dreifuss muscular dystrophy’. Nat. Genet. 8 (4), 323–327. doi:10.1038/ng1294-323
Bione, S., Small, K., Aksmanovic, V. M., D'Urso, M., CiccodicolA, A., MerLini, L., et al. (1995). ‘Identification of new mutations in the Emery-Dreifuss muscular dystrophy gene and evidence for genetic heterogeneity of the disease’. Hum. Mol. Genet. 4 (10), 1859–1863. doi:10.1093/hmg/4.10.1859
Bonne, G., Mercuri, E., Muchir, A., UrtizbereA, A., Becane, H. M., Recan, D., et al. (2000). ‘Clinical and molecular genetic spectrum of autosomal dominant Emery-Dreifuss muscular dystrophy due to mutations of the lamin A/C gene’. Ann. Neurology 48 (2), 170–180. doi:10.1002/1531-8249(200008)48:2<170:aid-ana6>3.0.co;2-j
Brachner, A., and Foisner, R. (2011). ‘Evolvement of LEM-proteins as chromatin tethers at the nuclear periphery’. Biochem. Soc. Trans. 39 (6), 1735–1741. doi:10.1042/BST20110724
Briand, N., and Collas, P. (2020). ‘Lamina-associated domains: Peripheral matters and internal affairs’. Genome Biol. 21 (1), 85. doi:10.1186/s13059-020-02003-5
Capetanaki, Y., Papathanasiou, S., Diokmetzidou, A., Vatsellas, G., and Tsikitis, M. (2015). Desmin related disease: A matter of cell survival failure. Curr. Opin. Cell. Biol. 32, 113–120. doi:10.1016/j.ceb.2015.01.004
Caravia, X. M., Ramirez-Martinez, A., Gan, P., Wang, F., McAnally, J. R., Xu, L., et al. (2022). Loss of function of the nuclear envelope protein LEMD2 causes DNA damage–dependent cardiomyopathy. J. Clin. Invest. 132, e158897. doi:10.1172/JCI158897
Cesarini, E., Mozzetta, C., Marullo, F., Gregoretti, F., Gargiulo, A., Columbaro, M., et al. (2015). ‘Lamin A/C sustains PcG protein architecture, maintaining transcriptional repression at target genes’. J. Cell. Biol. 211 (3), 533–551. doi:10.1083/jcb.201504035
Chancellor, T. J., Lee, J., Thodeti, C. K., and Lele, T. (2010). ‘Actomyosin tension exerted on the nucleus through nesprin-1 connections influences endothelial cell adhesion, migration, and cyclic strain-induced reorientation’. Biophysical J. 99 (1), 115–123. doi:10.1016/j.bpj.2010.04.011
Claeys, K. G., Fardeau, M., SchRodeR, R., Suominen, T., TolKsdorf, K., Behin, A., et al. (2008). ‘Electron microscopy in myofibrillar myopathies reveals clues to the mutated gene’. Neuromuscul. Disord. 18 (8), 656–666. doi:10.1016/j.nmd.2008.06.367
Cook, J. R., Carta, L., Benard, L., Chemaly, E. R., Chiu, E., Rao, S. K., et al. (2014). ‘Abnormal muscle mechanosignaling triggers cardiomyopathy in mice with Marfan syndrome’. J. Clin. Investigation 124 (3), 1329–1339. doi:10.1172/JCI71059
Crisp, M., Liu, Q., Roux, K., Rattner, J. B., Shanahan, C., Burke, B., et al. (2006). Coupling of the nucleus and cytoplasm: Role of the LINC complex. J. Cell. Biol. 172 (1), 41–53. doi:10.1083/jcb.200509124
Cronshaw, J. M., Krutchinsky, A. N., Zhang, W., Chait, B. T., and Matunis, M. J. (2002). ‘Proteomic analysis of the mammalian nuclear pore complex’. J. Cell. Biol. 158 (5), 915–927. doi:10.1083/jcb.200206106
Dechat, T., Gotzmann, J., Stockinger, A., Harris, C. A., Talle, M. A., Siekierka, J. J., et al. (1998). ‘Detergent-salt resistance of LAP2alpha in interphase nuclei and phosphorylation-dependent association with chromosomes early in nuclear assembly implies functions in nuclear structure dynamics.’. EMBO J. 17 (16), 4887–4902. doi:10.1093/emboj/17.16.4887
Dechat, T., Vlcek, S., and Foisner, R. (2000a). ‘Review: Lamina-associated polypeptide 2 isoforms and related proteins in cell cycle-dependent nuclear structure dynamics’. J. Struct. Biol. 129 (2–3), 335–345. doi:10.1006/jsbi.2000.4212
Dechat, T., KorBei, B., Vaughan, O. A., Vlcek, S., Hutchison, C. J., and FoisneR, R. (2000b). Lamina-associated polypeptide 2alpha binds intranuclear A-type lamins. J. Cell. Sci. 113 Pt 19, 3473–3484. doi:10.1242/jcs.113.19.3473
Demmerle, J., Koch, A. J., and Holaska, J. M. (2012). ‘The nuclear envelope protein emerin binds directly to histone deacetylase 3 (HDAC3) and activates HDAC3 activity’. J. Biol. Chem. 287 (26), 22080–22088. doi:10.1074/jbc.M111.325308
Ding, X., Xu, R., Yu, J., Xu, T., Zhuang, Y., and Han, M. (2007). ‘SUN1 is required for telomere attachment to nuclear envelope and gametogenesis in mice’. Dev. Cell. 12 (6), 863–872. doi:10.1016/j.devcel.2007.03.018
Donnaloja, F., JacchEtti, E., Soncini, M., and Raimondi, M. T. (2019). ‘Mechanosensing at the nuclear envelope by nuclear pore complex stretch activation and its effect in Physiology and pathology’. Front. Physiology 10, 896. doi:10.3389/fphys.2019.00896
Dupont, S., Morsut, L., Aragona, M., Enzo, E., Giulitti, S., Cordenonsi, M., et al. (2011). ‘Role of YAP/TAZ in mechanotransduction’. Nature 474 (7350), 179–183. doi:10.1038/nature10137
Earle, A. J., Kirby, T. J., Fedorchak, G. R., Isermann, P., Patel, J., Iruvanti, S., et al. (2020). Mutant lamins cause nuclear envelope rupture and DNA damage in skeletal muscle cells. Nat. Mat. 19, 464–473. doi:10.1038/s41563-019-0563-5
Essawy, N., Samson, C., Petitalot, A., Moog, S., Bigot, A., Herrada, I., et al. (2019). ‘An emerin LEM-domain mutation impairs cell response to mechanical stress’. Cells 8 (6), 570. doi:10.3390/cells8060570
Fernandez, A., Bautista, M., Wu, L., and Pinaud, F. (2022). ‘Emerin self-assembly and nucleoskeletal coupling regulate nuclear envelope mechanics against stress’. J. Cell. Sci. 135, jcs258969. doi:10.1242/jcs.258969
Fichtman, B., and Harel, A. (2014). Stress and aging at the nuclear gateway. Mech. Ageing. Dev. 135, 24–32. doi:10.1016/j.mad.2014.01.003
Fichtman, B., Zagairy, F., Biran, N., Barsheshet, Y., Chervinsky, E., Ben Neriah, Z., et al. (2019). ‘Combined loss of LAP1B and LAP1C results in an early onset multisystemic nuclear envelopathy’. Nat. Commun. 10 (1), 605. doi:10.1038/s41467-019-08493-7
Fidziańska, A., and Hausmanowa-Petrusewicz, I. (2003). ‘Architectural abnormalities in muscle nuclei. Ultrastructural differences between X-linked and autosomal dominant forms of EDMD’. J. Neurological Sci. 210 (1–2), 47–51. doi:10.1016/s0022-510x(03)00012-1
Foisner, R., and Gerace, L. (1993). ‘Integral membrane proteins of the nuclear envelope interact with lamins and chromosomes, and binding is modulated by mitotic phosphorylation’. Cell. 73 (7), 1267–1279. doi:10.1016/0092-8674(93)90355-t
Frock, R. L., Kudlow, B. A., Evans, A. M., Jameson, S. A., Hauschka, S. D., and Kennedy, B. K. (2006). Lamin A/C and emerin are critical for skeletal muscle satellite cell differentiation. Genes. Dev. 20 (4), 486–500. doi:10.1101/gad.1364906
Galata, Z., Kloukina, I., Kostavasili, I., Varela, A., Davos, C. H., Makridakis, M., et al. (2018). ‘Amelioration of desmin network defects by αβ-crystallin overexpression confers cardioprotection in a mouse model of dilated cardiomyopathy caused by LMNA gene mutation. J. Mol. Cell. Cardiol. 125, 74–86. doi:10.1016/j.yjmcc.2018.10.017
Gallo, S., Vitacolonna, A., Bonzano, A., Comoglio, P., and Crepaldi, T. (2019). ‘ERK: A key player in the pathophysiology of cardiac hypertrophy’,. Int. J. Mol. Sci. 20 (9), 2164. doi:10.3390/ijms20092164
Georgatos, S. D., Weber, K., Geisler, N., and Blobel, G. (1987). Binding of two desmin derivatives to the plasma membrane and the nuclear envelope of avian erythrocytes: Evidence for a conserved site-specificity in intermediate filament-membrane interactions. Proc. Natl. Acad. Sci. U. S. A. 84 (19), 6780–6784. doi:10.1073/pnas.84.19.6780
Gesson, K., Vidak, S., and Foisner, R. (2014). ‘Lamina-associated polypeptide (LAP)2α and nucleoplasmic lamins in adult stem cell regulation and disease’. Seminars Cell. & Dev. Biol. 29, 116–124. doi:10.1016/j.semcdb.2013.12.009
Ghosh, S., Seelbinder, B., Henderson, J. T., Watts, R. D., Scott, A. K., Veress, A. I., et al. (2019). Deformation microscopy for dynamic intracellular and intranuclear mapping of mechanics with high spatiotemporal resolution. Cell. Rep. 27, 1607–1620.e4. doi:10.1016/j.celrep.2019.04.009
Goodchild, R. E., and Dauer, W. T. (2005). ‘The AAA+ protein torsinA interacts with a conserved domain present in LAP1 and a novel ER protein’. J. Cell. Biol. 168 (6), 855–862. doi:10.1083/jcb.200411026
Guilluy, C., Osborne, L. D., Van Landeghem, L., Sharek, L., Superfine, R., Garcia-Mata, R., et al. (2014). Isolated nuclei adapt to force and reveal a mechanotransduction pathway in the nucleus. Nat. Cell. Biol. 16 (4), 376–381. doi:10.1038/ncb2927
Han, M., Zhao, M., Cheng, C., Huang, Y., Han, S., Li, W., et al. (2019). ‘Lamin A mutation impairs interaction with nucleoporin NUP155 and disrupts nucleocytoplasmic transport in atrial fibrillation’. Hum. Mutat. 40 (3), 310–325. doi:10.1002/humu.23691
Haque, F., Mazzeo, D., Patel, J. T., Smallwood, D. T., Ellis, J. A., Shanahan, C. M., et al. (2010). ‘Mammalian SUN protein interaction networks at the inner nuclear membrane and their role in laminopathy disease processes’. J. Biol. Chem. 285 (5), 3487–3498. doi:10.1074/jbc.M109.071910
Heffler, J., Shah, P. P., Robison, P., Phyo, S., Veliz, K., Uchida, K., et al. (2020). A balance between intermediate filaments and microtubules maintains nuclear architecture in the cardiomyocyte. Circ. Res. 126, E10–E26. doi:10.1161/CIRCRESAHA.119.315582
Hnia, K., Ramspacher, C., Vermot, J., and Laporte, J. (2015). Desmin in muscle and associated diseases: Beyond the structural function. Cell. Tissue Res. 360 (3), 591–608. doi:10.1007/s00441-014-2016-4
Hurt, E., and Beck, M. (2015). ‘Towards understanding nuclear pore complex architecture and dynamics in the age of integrative structural analysis’. Curr. Opin. Cell. Biol. 34, 31–38. doi:10.1016/j.ceb.2015.04.009
Jabre, S., Hleihel, W., and Coirault, C. (2021). Nuclear mechanotransduction in skeletal muscle. Cells 10, 318. doi:10.3390/cells10020318
Jahed, Z., Vu, U. T., Fadavi, D., Ke, H., Rathish, A., Kim, S. C. J., et al. (2018a). ‘A molecular model for LINC complex regulation: Activation of SUN2 for KASH binding’. Mol. Biol. Cell. 29 (16), 2012–2023. doi:10.1091/mbc.E18-04-0266
Jahed, Z., Fadavi, D., Vu, U. T., Asgari, E., Luxton, G. W. G., and Mofrad, M. R. K. (2018b). ‘Molecular insights into the mechanisms of SUN1 oligomerization in the nuclear envelope’. Biophysical J. 114 (5), 1190–1203. doi:10.1016/j.bpj.2018.01.015
Janin, A., and Gache, V. (2018). Nesprins and lamins in health and diseases of cardiac and skeletal muscles. Front. Physiol. 9, 1277. doi:10.3389/fphys.2018.01277
Jungwirth, M., Dear, M. L., Brown, P., Holbrook, K., and Goodchild, R. (2010). ‘Relative tissue expression of homologous torsinB correlates with the neuronal specific importance of DYT1 dystonia-associated torsinA’. Hum. Mol. Genet. 19 (5), 888–900. doi:10.1093/hmg/ddp557
Kalukula, Y., Stephens, A. D., Lammerding, J., and Gabriele, S. (2022). Mechanics and functional consequences of nuclear deformations. Nat. Rev. Mol. Cell. Biol. 23, 583–602. doi:10.1038/s41580-022-00480-z
Kayman-Kurekci, G., Talim, B., Korkusuz, P., Sayar, N., Sarioglu, T., Oncel, I., et al. (2014). ‘Mutation in TOR1AIP1 encoding LAP1B in a form of muscular dystrophy: A novel gene related to nuclear envelopathies’,. Neuromuscul. Disord. 24 (7), 624–633. doi:10.1016/j.nmd.2014.04.007
Ketema, M., and Sonnenberg, A. (2011). ‘Nesprin-3: A versatile connector between the nucleus and the cytoskeleton’. Biochem. Soc. Trans. 39 (6), 1719–1724. doi:10.1042/BST20110669
Khalilimeybodi, A., Riaz, M., Campbell, S. G., Omens, J. H., McCulloch, A. D., Qyang, Y., et al. (2023). Signaling network model of cardiomyocyte morphological changes in familial cardiomyopathy. J. Mol. Cell. Cardiol. 174, 1–14. doi:10.1016/j.yjmcc.2022.10.006
Kim, C. E., Perez, A., Perkins, G., Ellisman, M. H., and Dauer, W. T. (2010). ‘A molecular mechanism underlying the neural-specific defect in torsinA mutant mice’. Proc. Natl. Acad. Sci. U. S. A. 107 (21), 9861–9866. doi:10.1073/pnas.0912877107
Kirkland, N. J., Skalak, S. H., Whitehead, A. J., Hocker, J. D., Beri, P., Vogler, G., et al. (2023). ‘Age-dependent Lamin changes induce cardiac dysfunction via dysregulation of cardiac transcriptional programs’. Nat. Aging 3, 17–33. doi:10.1038/s43587-022-00323-8
Knöll, R., Hoshijima, M., Hoffman, H. M., Person, V., Lorenzen-Schmidt, I., Bang, M. L., et al. (2002). ‘The cardiac mechanical stretch sensor machinery involves a Z disc complex that is defective in a subset of human dilated cardiomyopathy’. Cell. 111 (7), 943–955. doi:10.1016/s0092-8674(02)01226-6
Kondo, Y., Kondoh, J., Hayashi, D., Ban, T., Takagi, M., Kamei, Y., et al. (2002). ‘Molecular cloning of one isotype of human lamina-associated polypeptide 1s and a topological analysis using its deletion mutants’. Biochem. Biophysical Res. Commun. 294 (4), 770–778. doi:10.1016/S0006-291X(02)00563-6
Kresh, J. Y., and Chopra, A. (2011). ‘Intercellular and extracellular mechanotransduction in cardiac myocytes’. Pflügers Archiv - Eur. J. Physiology 462 (1), 75–87. doi:10.1007/s00424-011-0954-1
Lammerding, J., Hsiao, J., Schulze, P. C., Kozlov, S., Stewart, C. L., and Lee, R. T. (2005). ‘Abnormal nuclear shape and impaired mechanotransduction in emerin-deficient cells’. J. Cell. Biol. 170 (5), 781–791. doi:10.1083/jcb.200502148
Lanzicher, T., Martinelli, V., Puzzi, L., Del Favero, G., Codan, B., Long, C. S., et al. (2015). The cardiomyopathy lamin A/C D192G mutation disrupts whole-cell biomechanics in cardiomyocytes as measured by atomic force microscopy loading-unloading curve analysis. Sci. Rep. 5, 13388. doi:10.1038/srep13388
Lanzuolo, C., Roure, V., Dekker, J., Bantignies, F., and Orlando, V. (2007). ‘Polycomb response elements mediate the formation of chromosome higher-order structures in the bithorax complex’. Nat. Cell. Biol. 9 (10), 1167–1174. doi:10.1038/ncb1637
Lee, K. K., Haraguchi, T., Lee, R. S., Koujin, T., Hiraoka, Y., and Wilson, K. L. (2001) ‘Distinct functional domains in emerin bind lamin A and DNA-bridging protein BAF’. J. Cell. Sci. 114, 4567–4573. doi:10.1242/jcs.114.24.4567
Lei, K., Zhang, X., Ding, X., Guo, X., Chen, M., Zhu, B., et al. (2009). ‘SUN1 and SUN2 play critical but partially redundant roles in anchoring nuclei in skeletal muscle cells in mice’. Proc. Natl. Acad. Sci. U. S. A. 106 (25), 10207–10212. doi:10.1073/pnas.0812037106
Li, P., and Noegel, A. A. (2015). ‘Inner nuclear envelope protein SUN1 plays a prominent role in mammalian mRNA export’. Nucleic Acids Res. 43 (20), 9874–9888. doi:10.1093/nar/gkv1058
Liao, L., Qu, R., Ouang, J., and Dai, J. (2019). ‘A glance at the nuclear envelope spectrin repeat protein 3’. BioMed Res. Int. 2019, 1651805. doi:10.1155/2019/1651805
Lin, F., Blake, D. L., Callebaut, I., Skerjanc, I. S., HoLmer, L., McBurney, M. W., et al. (2000). ‘MAN1, an inner nuclear membrane protein that shares the LEM domain with lamina-associated polypeptide 2 and emerin’. J. Biol. Chem. 275 (7), 4840–4847. doi:10.1074/jbc.275.7.4840
Liu, Q., Pante, N., Misteli, T., Elsagga, M., Crisp, M., Hodzic, D., et al. (2007). ‘Functional association of Sun1 with nuclear pore complexes’. J. Cell. Biol. 178 (5), 785–798. doi:10.1083/jcb.200704108
Lockard, V. G., and Bloom, S. (1993). Trans-cellular desmin-lamin B intermediate filament network in cardiac myocytes. J. Mol. Cell. Cardiol. 25 (3), 303–309. doi:10.1006/jmcc
Lorber, D., Rotkopf, R., and Volk, T. (2020). A minimal constraint device for imaging nuclei in live Drosophila contractile larval muscles reveals novel nuclear mechanical dynamics. Lab. Chip 20, 2100–2112. doi:10.1039/d0lc00214c
Luithle, N., Uit de Bos, J., Hovius, R., Maslennikova, D., Tm Lewis, R., Ungricht, R., et al. (2020). “‘Torsin ATPases influence chromatin interaction of the Torsin regulator LAP1’,” in eLife. Editors M. C. King, A. Akhmanova, and M. C. King, 9, e63614. doi:10.7554/eLife.63614
Lyon, R. C., Zanella, F., Omens, J. H., and Sheikh, F. (2015). ‘Mechanotransduction in cardiac hypertrophy and failure’. Circulation Res. 116 (8), 1462–1476. doi:10.1161/CIRCRESAHA.116.304937
Manila, S., Recan, D., Sewry, C. A., Hoeltzenbein, M., LlenSe, S., Leturcq, F., et al. (1998). ‘Mutations in emery-dreifuss muscular dystrophy and their effects on emerin protein expression’. Hum. Mol. Genet. 7 (5), 855–864. doi:10.1093/hmg/7.5.855
Marullo, F., Cesarini, E., AntoneLLi, L., Gregoretti, F., Oliva, G., and Lanzuolo, C. (2016). ‘Nucleoplasmic Lamin A/C and Polycomb group of proteins: An evolutionarily conserved interplay’. Nucleus 7 (2), 103–111. doi:10.1080/19491034.2016.1157675
Matsuda, A., and Mofrad, M. R. K. (2022). ‘On the nuclear pore complex and its emerging role in cellular mechanotransduction’. Apl. Bioeng. 6 (1), 011504. doi:10.1063/5.0080480
Maurer, M., and Lammerding, J. (2019). The driving force: Nuclear mechanotransduction in cellular function, fate, and disease. Annu. Rev. Biomed. Eng. 21, 443–468. doi:10.1146/annurev-bioeng-060418-052139
Meinke, P., Mattioli, E., Haque, F., Antoku, S., Columbaro, M., Straatman, K. R., et al. (2014). ‘Muscular dystrophy-associated SUN1 and SUN2 variants disrupt nuclear-cytoskeletal connections and myonuclear organization’. PLoS Genet. 10, e1004605. doi:10.1371/journal.pgen.1004605
Melcon, G., Kozlov, S., Cutler, D. A., Sullivan, T., Hernandez, L., Zhao, P., et al. (2006). ‘Loss of emerin at the nuclear envelope disrupts the Rb1/E2F and MyoD pathways during muscle regeneration’. Hum. Mol. Genet. 15 (4), 637–651. doi:10.1093/hmg/ddi479
Mewborn, S. K., Puckelwartz, M. J., Abuisneineh, F., Fahrenbach, J. P., Zhang, Y., MacLeod, H., et al. (2010). ‘Altered chromosomal positioning, compaction, and gene expression with a lamin A/C gene mutation’. PLoS ONE 5, e14342. doi:10.1371/journal.pone.0014342
Mislow, J. M. K., Holaska, J. M., Kim, M. S., Lee, K. K., Segura-Totten, M., Wilson, K. L., et al. (2002). Nesprin-1alpha self-associates and binds directly to emerin and lamin A in vitro. FEBS Lett. 525 (1–3), 135–140. doi:10.1016/s0014-5793(02)03105-8
Mofrad, M. R. K., and Kamm, R. D. (2009). Cellular mechanotransduction: Diverse perspectives from molecules to tissues. Cambridge University Press.
Mounkes, L. C., Kozlov, S. V., Rottman, J. N., and Stewart, C. L. (2005). ‘Expression of an LMNA-N195K variant of A-type lamins results in cardiac conduction defects and death in mice’. Hum. Mol. Genet. 14 (15), 2167–2180. doi:10.1093/hmg/ddi221
Naetar, N., Korbei, B., Kozlov, S., Kerenyi, M. A., Dorner, D., Kral, R., et al. (2008). ‘Loss of nucleoplasmic LAP2alpha-lamin A complexes causes erythroid and epidermal progenitor hyperproliferation’. Nat. Cell. Biol. 10 (11), 1341–1348. doi:10.1038/ncb1793
Nikolova, V., Leimena, C., McMahon, A. C., Tan, J. C., Chandar, S., Jogia, D., et al. (2004). Defects in nuclear structure and function promote dilated cardiomyopathy in lamin A/C-deficient mice. J. Clin. Invest. 113 (3), 357–369. doi:10.1172/JCI19448
Ognibene, A., Sabatelli, P., Petrini, S., Squarzoni, S., Riccio, M., Santi, S., et al. (1999). ‘Nuclear changes in a case of X-linked Emery-Dreifuss muscular dystrophy’. Muscle & Nerve 22 (7), 864–869. doi:10.1002/(sici)1097-4598(199907)22:7<864:aid-mus8>3.0.co;2-g
Osmanagic-Myers, S., Dechat, T., and Foisner, R. (2015). ‘Lamins at the crossroads of mechanosignaling’. Genes. & Dev. 29 (3), 225–237. doi:10.1101/gad.255968.114
Owens, D. J., Fischer, M., Jabre, S., Moog, S., Mamchaoui, K., Butler-Browne, G., et al. (2020). Lamin mutations cause increased YAP nuclear entry in muscle stem cells. Cells 9, 816. doi:10.3390/cells9040816
Ozawa, R., Hayashi, Y. K., Ogawa, M., Kurokawa, R., Matsumoto, H., Noguchi, S., et al. (2006). ‘Emerin-Lacking mice show minimal motor and cardiac dysfunctions with nuclear-associated vacuoles’. Am. J. Pathology 168 (3), 907–917. doi:10.2353/ajpath.2006.050564
Pan, D., Estevez-Salmeron, L. D., Stroschein, S. L., Zhu, X., He, J., Zhou, S., et al. (2005). The integral inner nuclear membrane protein MAN1 physically interacts with the R-Smad proteins to repress signaling by the transforming growth factor-{beta} superfamily of cytokines. J. Biol. Chem. 280 (16), 15992–16001. doi:10.1074/jbc.M411234200
Pekovic, V., Harborth, J., Broers, J. L. V., Ramaekers, F. C. S., van Engelen, B., Lammens, M., et al. (2007). Nucleoplasmic LAP2alpha-lamin A complexes are required to maintain a proliferative state in human fibroblasts. J. Cell. Biol. 176 (2), 163–172. doi:10.1083/jcb.200606139
Powers, J. D., Malingen, S. A., Regnier, M., and Daniel, T. L. (2021). The sliding filament theory since andrew huxley: Multiscale and multidisciplinary muscle research. Annu. Rev. Biophys. 50, 373–400. doi:10.1146/annurev-biophys-110320-062613
Powers, J. D., Kirkland, N. J., Liu, C., Razu, S. S., Fang, X., Engler, A. J., et al. (2022). Subcellular remodeling in filamin C deficient mouse hearts impairs myocyte tension development during progression of dilated cardiomyopathy. Int. J. Mol. Sci. 23, 871. doi:10.3390/ijms23020871
Powers, J. D., and McCulloch, A. D. (2022). Biomechanical signals regulating the structure of the heart. Curr. Opin. Physiol. 25, 100482. doi:10.1016/j.cophys.2021.100482
Puckelwartz, M. J., Kessler, E. J., Kim, G., Dewitt, M. M., Zhang, Y., Earley, J. U., et al. (2010). ‘Nesprin-1 mutations in human and murine cardiomyopathy’. J. Mol. Cell. Cardiol. 48 (4), 600–608. doi:10.1016/j.yjmcc.2009.11.006
Puckelwartz, M. J., Kessler, E., Zhang, Y., Hodzic, D., Randles, K. N., Morris, G., et al. (2009). ‘Disruption of nesprin-1 produces an Emery Dreifuss muscular dystrophy-like phenotype in mice’. Hum. Mol. Genet. 18 (4), 607–620. doi:10.1093/hmg/ddn386
Ramirez-Martinez, A., Zhang, Y., Chen, K., Kim, J., Cenik, B. K., McAnally, J. R., et al. (2021). The nuclear envelope protein Net39 is essential for muscle nuclear integrity and chromatin organization. Nat. Commun. 12, 690. doi:10.1038/s41467-021-20987-x
Roman, W., Martins, J. P., Carvalho, F. A., Voituriez, R., Abella, J. V. G., Santos, N. C., et al. (2017). Myofibril contraction and crosslinking drive nuclear movement to the periphery of skeletal muscle. Nat. Cell. Biol. 19, 1189–1201. doi:10.1038/ncb3605
Roman, W., Pinheiro, H., Pimentel, M. R., Segalés, J., Oliveira, L. M., García-Domínguez, E., et al. (2021). Muscle repair after physiological damage relies on nuclear migration for cellular reconstruction. Science 374, 355–359. doi:10.1126/science.abe5620
Ross, J. A., and Stroud, M. J. (2021). The nucleus: Mechanosensing in cardiac disease. Int. J. Biochem. Cell. Biol. 137, 106035. doi:10.1016/j.biocel.2021.106035
Santos, M., Domingues, S. C., Costa, P., Muller, T., Galozzi, S., Marcus, K., et al. (2014). ‘Identification of a novel human LAP1 isoform that is regulated by protein phosphorylation’. PLoS ONE 9, e113732. doi:10.1371/journal.pone.0113732
Saucerman, J. J., Tan, P. M., Buchholz, K. S., McCulloch, A. D., and Omens, J. H. (2019). Mechanical regulation of gene expression in cardiac myocytes and fibroblasts. Nat. Rev. Cardiol. 16, 361–378. doi:10.1038/s41569-019-0155-8
Sebillon, P., BouChier, C., Bidot, L. D., Bonne, G., Ahamed, K., Charron, P., et al. (2003). ‘Expanding the phenotype of LMNA mutations in dilated cardiomyopathy and functional consequences of these mutations’. J. Med. Genet. 40 (8), 560–567. doi:10.1136/jmg.40.8.560
Shah, P. P., Lv, W., Rhoades, J. H., Poleshko, A., Abbey, D., Caporizzo, M. A., et al. (2021). Pathogenic LMNA variants disrupt cardiac lamina-chromatin interactions and de-repress alternative fate genes. Cell. Stem Cell. 28, 938–954.e9. doi:10.1016/j.stem.2020.12.016
Shin, J.-Y., Mendez-Lopez, I., Wang, Y., Hays, A. P., Tanji, K., Lefkowitch, J. H., et al. (2013) ‘Lamina-associated polypeptide-1 interacts with the muscular dystrophy protein emerin and is essential for skeletal muscle maintenance’, Dev. Cell., 26(6), pp. 591–603. : doi:10.1016/j.devcel.2013.08.012
Shin, J.-Y., Le Dour, C., Sera, F., Iwata, S., Homma, S., Joseph, L. C., et al. (2014). ‘Depletion of lamina-associated polypeptide 1 from cardiomyocytes causes cardiac dysfunction in mice’. Nucleus 5 (3), 260–459. doi:10.4161/nucl.29227
Sosa, B. A., Rothballer, A., Kutay, U., and Schwartz, T. U. (2012). LINC complexes form by binding of three KASH peptides to domain interfaces of trimeric SUN proteins. Cell. 149 (5), 1035–1047. doi:10.1016/j.cell.2012.03.046
Sosa Ponce, M. L., Moradi-Fard, S., Zaremberg, V., and Cobb, J. A. (2020). ‘SUNny ways: The role of the SUN-domain protein Mps3 bridging yeast nuclear organization and lipid homeostasis’. Front. Genet. 11. doi:10.3389/fgene.2020.00136
Stroud, M. J. (2018). Linker of nucleoskeleton and cytoskeleton complex proteins in cardiomyopathy. Biophys. Rev. 10, 1033–1051. doi:10.1007/s12551-018-0431-6
Stroud, M. J., Banerjee, I., Veevers, J., and Chen, J. (2014). Linker of nucleoskeleton and cytoskeleton complex proteins in cardiac structure, function, and disease. Circ. Res. 114, 538–548. doi:10.1161/CIRCRESAHA.114.301236
Stubenvoll, A., Rice, M., Wietelmann, A., Wheeler, M., and Braun, T. (2015). ‘Attenuation of Wnt/β-catenin activity reverses enhanced generation of cardiomyocytes and cardiac defects caused by the loss of emerin’. Hum. Mol. Genet. 24 (3), 802–813. doi:10.1093/hmg/ddu498
Tan, P. M., Buchholz, K. S., Omens, J. H., McCulloch, A. D., and Saucerman, J. J. (2017). Predictive model identifies key network regulators of cardiomyocyte mechanosignaling. PLoS Comput. Biol. 13, e1005854. doi:10.1371/journal.pcbi.1005854
Tarazón, E., Rivera, M., Roselló-Lletí, E., Molina-Navarro, M. M., Sánchez-Lázaro, I. J., España, F., et al. (2012). ‘Heart failure induces significant changes in nuclear pore complex of human cardiomyocytes’. PLOS ONE 7, e48957. doi:10.1371/journal.pone.0048957
Taylor, M. R. G., Slavov, D., Gajewski, A., Vlcek, S., Ku, L., Fain, P. R., et al. (2005). ‘Thymopoietin (lamina-associated polypeptide 2) gene mutation associated with dilated cardiomyopathy’. Hum. Mutat. 26 (6), 566–574. doi:10.1002/humu.20250
Thomas, C. H., Collier, J. H., Sfeir, C. S., and Healy, K. E. (2002). Engineering gene expression and protein synthesis by modulation of nuclear shape. Proc. Natl. Acad. Sci. 99, 1972–1977. doi:10.1073/pnas.032668799
Tsikitis, M., Galata, Z., Mavroidis, M., Psarras, S., and Capetanaki, Y. (2018). Intermediate filaments in cardiomyopathy. Biophys. Rev. 10 (4), 1007–1031. doi:10.1007/s12551-018-0443-2
Velasquez, L. S., Sutherland, L. B., Liu, Z., Grinnell, F., Kamm, K. E., Schneider, J. W., et al. (2013). ‘Activation of MRTF-A–dependent gene expression with a small molecule promotes myofibroblast differentiation and wound healing’. Proc. Natl. Acad. Sci. U. S. A. 110 (42), 16850–16855. doi:10.1073/pnas.1316764110
Vidak, S., Georgiou, K., Fichtinger, P., Naetar, N., Dechat, T., and Foisner, R. (2018). ‘Nucleoplasmic lamins define growth-regulating functions of lamina-associated polypeptide 2α in progeria cells’. J. Cell. Sci. 131, jcs208462. doi:10.1242/jcs.208462
Vrabie, A., Goldfarb, L. G., Shatunov, A., Nagele, A., Fritz, P., Kaczmarek, I., et al. (2005). ‘The enlarging spectrum of desminopathies: New morphological findings, eastward geographic spread, novel exon 3 desmin mutation’. Acta Neuropathol. 109 (4), 411–417. doi:10.1007/s00401-005-0980-1
Vytopil, M., Benedetti, S., Ricci, E., Galluzzi, G., Dello Russo, A., Merlini, L., et al. (2003). ‘Mutation analysis of the lamin A/C gene (LMNA) among patients with different cardiomuscular phenotypes’. J. Med. Genet. 40, e132. doi:10.1136/jmg.40.12.e132
Wada, K.-I., Itoga, K., Okano, T., Yonemura, S., and Sasaki, H. (2011). Hippo pathway regulation by cell morphology and stress fibers. Engl. 138 (18), 3907–3914. doi:10.1242/dev.070987
Walker, C. J., Crocini, C., Ramirez, D., Killaars, A. R., Grim, J. C., Aguado, B. A., et al. (2021). Nuclear mechanosensing drives chromatin remodelling in persistently activated fibroblasts. Nat. Biomed. Eng. 5, 1485–1499. doi:10.1038/s41551-021-00709-w
Wang, W., Shi, Z., Jiao, S., Chen, C., Wang, H., Liu, G., et al. (2012). ‘Structural insights into SUN-KASH complexes across the nuclear envelope’. Cell. Res. 22 (10), 1440–1452. doi:10.1038/cr.2012.126
Wilhelmsen, K., Litjens, S. H. M., Kuikman, I., Tshimbalanga, N., Janssen, H., van den Bout, I., et al. (2005). ‘Nesprin-3, a novel outer nuclear membrane protein, associates with the cytoskeletal linker protein plectin’. J. Cell. Biol. 171 (5), 799–810. doi:10.1083/jcb.200506083
Wilson, K. L., and Foisner, R. (2010). ‘Lamin-binding proteins’. Cold Spring Harb. Perspect. Biol. 2, a000554. doi:10.1101/cshperspect.a000554
Wood, N. B., Kelly, C. M., O’Leary, T. S., Martin, J. L., and Previs, M. J. (2022). Cardiac myosin filaments are maintained by stochastic protein replacement. Mol. Cell. Proteomics 21, 100274. doi:10.1016/j.mcpro.2022.100274
Yadav, S., Ta, H. T., and Nguyen, N.-T. (2021). ‘Mechanobiology in cardiology: Micro- and nanotechnologies to probe mechanosignaling’. VIEW 2 (2), 20200080. doi:10.1002/viw.20200080
Yang, J., Argenziano, M. A., Angulo, M. B., Bertalovitz, A., Beidokhti, M. N., and McDonald, T. V. (2021). Phenotypic variability in iPSC-induced cardiomyocytes and cardiac fibroblasts carrying diverse LMNA mutations. Front. Physiol. 12, 778982. doi:10.3389/fphys.2021.778982
Zhang, Q., Bethmann, C., Worth, N. F., Davies, J. D., Wasner, C., Feuer, A., et al. (2007). ‘Nesprin-1 and -2 are involved in the pathogenesis of Emery Dreifuss muscular dystrophy and are critical for nuclear envelope integrity’. Hum. Mol. Genet. 16 (23), 2816–2833. doi:10.1093/hmg/ddm238
Zhang, J., Felder, A., Liu, Y., Guo, L. T., Lange, S., Dalton, N. D., et al. (2010). ‘Nesprin 1 is critical for nuclear positioning and anchorage’. Hum. Mol. Genet. 19 (2), 329–341. doi:10.1093/hmg/ddp499
Zhang, S., Schones, D. E., Malicet, C., Rochman, M., Zhou, M., Foisner, R., et al. (2013). ‘High mobility group protein N5 (HMGN5) and lamina-associated polypeptide 2α (LAP2α) interact and reciprocally affect their genome-wide chromatin organization’. J. Biol. Chem. 288 (25), 18104–18109. doi:10.1074/jbc.C113.469544
Zheng, X., Hu, J., Yue, S., Kristiani, L., Kim, M., Sauria, M., et al. (2018). ‘Lamins organize the global three-dimensional genome from the nuclear periphery’. Mol. Cell. 71 (5), 802–815.e7. doi:10.1016/j.molcel.2018.05.017
Zhou, L., and Panté, N. (2010). ‘The nucleoporin Nup153 maintains nuclear envelope architecture and is required for cell migration in tumor cells’. FEBS Lett. 584 (14), 3013–3020. doi:10.1016/j.febslet.2010.05.038
Zhou, C., Rao, L., Shanahan, C. M., and Zhang, Q. (2018). ‘Nesprin-1/2: Roles in nuclear envelope organisation, myogenesis and muscle disease’. Biochem. Soc. Trans. 46 (2), 311–320. doi:10.1042/BST20170149
Zhou, Y., Chen, Z., Zhang, L., Zhu, M., Tan, C., Zhou, X., et al. (2020). Loss of filamin C is catastrophic for heart function. Circulation 141, 869–871. doi:10.1161/CIRCULATIONAHA.119.044061
Keywords: mechanosignaling, nucleus, myocytes, nucleoskeleton, nuclear morphology, LINC complex, cardiomyopathy, laminopathy
Citation: Zhang B, Powers JD, McCulloch AD and Chi NC (2023) Nuclear mechanosignaling in striated muscle diseases. Front. Physiol. 14:1126111. doi: 10.3389/fphys.2023.1126111
Received: 17 December 2022; Accepted: 22 February 2023;
Published: 07 March 2023.
Edited by:
Kenneth Scott Campbell, University of Kentucky, United StatesReviewed by:
Mary Tsikitis, Biomedical Research Foundation of the Academy of Athens (BRFAA), GreeceMatthew Caporizzo, University of Vermont, United States
Copyright © 2023 Zhang, Powers, McCulloch and Chi. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Neil C. Chi, nchi@health.ucsd.edu