Visualized peritoneal fluid variation in adolescents and young adults with endometriosis: is there more to it?

Introduction

Endometriosis is a chronic inflammatory disorder, often presenting with severe pelvic pain and infertility, that affects approximately 10% of women in their reproductive years globally (1). Endometriosis is characterized by growth of endometrial-like stroma and glands outside the endometrium, with ectopic endometriosis lesions often exhibiting as superficial peritoneal lesions, endometriomas, or deep endometriosis in the pelvic cavity (1).

Peritoneal fluid is a biologic medium within the peritoneal cavity, which can be observed and collected during laparoscopic surgery. While peritoneal fluid is derived by transudation from blood and therefore many substances found in blood can also be detected in the peritoneal fluid (2), the location of peritoneal fluid in the pelvis proximal to ectopic endometriotic lesions makes it a medium that contributes to defining the local environment of the peritoneal cavity. Therefore, peritoneal fluid biomarkers have been studied to understand endometriosis pathophysiology and its biomarkers (3, 4). However, only a few studies have reported the gross characteristics of peritoneal fluid in endometriosis, with inconsistent findings (5–7). There may be many implications for influence of gross characteristics on endometriosis research and biomarker discovery. One pragmatic example is that patients with a higher peritoneal volume at any given surgery could, by definition, have their fluid included more frequently in experiments and observational studies. The objective of this study was to examine the association between gross characteristics of peritoneal fluid (i.e., color and volume) and patient-specific characteristics, including demographics, symptoms, pharmaceutical exposures, menstrual cycle phase, and visualized disease at the time of surgery.

Materials and methods

Study population

The Women's Health Study: from Adolescence to Adulthood (A2A) is an observational longitudinal cohort study that enrolled 1,549 pre-menopausal female participants with and without endometriosis [n = 787 with surgically-confirmed endometriosis and n = 762 without] between November 2012 and June 2018. Details of the study were described previously (8). In brief, participants were recruited from Gynecology and Adolescent Medicine clinics within two tertiary care centers, as well as from the surrounding community via local advertisements, online postings, and word of mouth. All participants were asked to complete an extensive questionnaire at baseline and annual follow-up questionnaires which were expanded from the World Endometriosis Research Foundation Endometriosis Phenome and Biobanking Harmonization Project (WERF EPHect) standard clinical questionnaire, assessing various factors including anthropometrics, behavioral and reproductive characteristics, pain symptom experience, medical history, and medication use (9). Of the 787 surgically-confirmed endometriosis patients enrolled in the A2A study, 608 had surgery at or after enrollment, and 543 had peritoneal fluid collected and had complete surgical forms with both peritoneal fluid color and volume recorded. Additionally, of the 762 participants enrolled without a diagnosis of surgically-confirmed endometriosis, two had a surgery after enrollment where endometriosis was confirmed and a peritoneal fluid sample was collected with complete surgical form data. Thus, this study included 545 surgically-confirmed endometriosis patients with both peritoneal fluid color and volume information at time of their endometriosis-related surgery.

This study was approved by the Boston Children's Hospital (BCH) Institutional Review Board on behalf of both BCH and Brigham and Women's Hospital. Written informed consent was obtained, with both parental consent and participant assent for individuals aged <18 years at enrollment.

Assessment of surgical phenotype

Each surgery was attended by a research assistant trained specifically for the study who, per dictation and confirmation by the surgeon, completed the WERF EPHect standard surgical form. This form captured endometriosis macro-phenotype (i.e., superficial peritoneal lesion, endometrioma, deep), revised American Society for Reproductive Medicine (rASRM) score, location of endometriotic lesions, appearance (or color) of superficial peritoneal endometriosis lesions, date of last menstrual period, vaginal bleeding on the day of surgery, and medication use in the past 48 h (10). Peritoneal fluid was collected at endometriosis-related surgeries following the WERF EPHect fluids collection standard protocol (11). Deviations from the standard EPHect protocol included that in this study the peritoneal fluid aspirate was housed in an incubator to mimic the body's core temperature until the specimen could be transported to the biorepository for processing. The peritoneal fluid was centrifuged at 300 g for 10 min at 4°C and frozen at −80°C using Mr. Frosty freezing container. The research assistant who received the peritoneal fluid in the laboratory recorded the gross characteristics of the peritoneal fluid samples (i.e., appearance or color and volume) before processing.

Other variables

Participant characteristics, including body mass index (BMI), race and ethnicity, and pain symptoms [i.e., dysmenorrhea (pain with periods), acyclic/general pelvic pain (pain occurring at times other than with menses), and dyspareunia (pain with vaginal intercourse/penetration)] were abstracted from baseline or annual questionnaires proximal to the endometriosis-related surgeries. BMI was calculated from self-reported weight and height. For women aged 20 years or older, participants were categorized as underweight (BMI < 18.5 kg/m²), normal-weight (BMI 18.5–24.9 kg/m²), overweight (BMI 25–29.9 kg/m²⁾, or obese (BMI ≥ 30 kg/m²) according to World Health Organization (WHO) criteria (12). For participants <20 years, the age- and gender-specific BMI Z-score was calculated, and participants were categorized as underweight (Z-score ≤−2), normal weight (Z-score >−2 to <1), overweight (Z-score 1–2), or obese (Z-score >2) (13). Usual severity of pelvic pain with periods or dysmenorrhea was assessed categorically as none, mild (medication never or rarely needed), moderate (medication usually needed), and severe (medication and bed rest needed) among those who self-reported they were cycling in the last 3 months. Any experience of acyclic pelvic pain was assessed in the last 3 months (yes/no), and dyspareunia was assessed by asking if participants aged 18 years or older avoided or interrupted intercourse or penetration in the last 12 months because of pelvic pain (yes/no).

Statistical methods

We examined the distribution of participant's surgical and clinical characteristics by the peritoneal fluid color (yellow, orange, pink, red) and volume (≤3, 4–6, 7–11, > 11 ml) at the time of their endometriosis-related surgery. For lesion appearance/color type, we created two grouped variables—type A (clear or red lesions) and type B (blue/black, brown, or white lesions), as these groups have been presumed to represent early vs. late lesions. Medications were classified as any antidepressant/antianxiety medication use or any pain medication use (analgesics or anticonvulsants), including both prescription and non-prescription medications. Chi-square or Fisher's exact tests were applied to test for differences across the categories. All statistics were conducted using SAS 9.4 (SAS Institute, Cary, NC).

Results

Our study population consisted mainly of adolescents and young adults with surgically confirmed with endometriosis (86% were age <25 years old; Table 1), with median age at endometriosis diagnosis being 16-years old. Majority were white race (89%) and were exposed to exogenous hormones at time of their endometriosis-related surgery (83%). The most prevalent hormonal medication used was the combined estrogen + progesterone birth control pills (61%). The majority of patients were found to have only superficial peritoneal lesions (95%) at surgery, which is the most common macro-phenotype of endometriosis (14).

Table 1

Table 1. Characteristics of surgically-confirmed endometriosis cases with gross peritoneal fluid characteristics in the A2A study (n = 545).

When we examined patient characteristics by peritoneal fluid colors, we did not see clear differences in exogenous hormone exposure status at time of surgery by peritoneal fluid color. However, among those who were on hormones, there were significant differences in the type of hormones used by peritoneal fluid color (p = 0.002, Table 2). Among those who were on hormones at surgery, endometriosis patients with orange, pink, or red color peritoneal fluid were more likely to be on combined birth control pills (65%, 62%, and 67% respectively) whereas those with yellow peritoneal fluid was more likely to be taking progesterone only formulation exposure (40%). When we examined pain medications and anti-depressants/anti-anxiety exposures, there were no statistically significant nor informative differences by peritoneal fluid color.

Table 2

Table 2. Patient characteristics, endometriosis type, lesion location, and color by peritoneal fluid color (n = 545).

We observed differences in menstrual cycle phase at time of laparoscopic surgery by peritoneal fluid color (p = 0.006). Among those who were cycling at time of surgery, endometriosis patients with red peritoneal fluid were most like to be in the proliferative phase (49%) while those with yellow or orange peritoneal fluid were most likely to be in the secretory phase (57% and 86% respectively). Consistently, there were significant differences in the patients' vaginal bleeding status at surgery by peritoneal fluid color (p < 0.0001), where endometriosis patients with red peritoneal fluid were more likely to be bleeding at time of surgery (34%) and less likely in other colors (4%–18%). We conducted a sensitivity analysis excluding those who reported bleeding at time of surgery (n = 124) and those for whom the peritoneal fluid sample was documented to have had gross contamination (n = 7), but observed similar results with similar distributions (Supplementary Table S1).

In terms of the endometriosis surgically visualized macro-phenotypes, which 95% of our patients had superficial peritoneal lesions only and rASRM stage I or II, there were no obvious patterns of difference in the endometriosis macro-phenotype or rASRM stage by peritoneal fluid color. However, pink and red color peritoneal fluid were suggestively more likely to be associated with rASRM stage III or IV (p = 0.16), although sample size was limited (n = 28 with rASRM stage III or IV).

Differences were observed in superficial peritoneal lesion appearance by peritoneal fluid color. Those with pink or red peritoneal fluid were slightly more likely to have any red lesions (88, 90%) than those with yellow or orange peritoneal fluid (79%, 71%; p = 0.001). Those with yellow peritoneal fluid were less likely to have type B lesions (i.e., blue/black, brown, or white; 44%) whereas those with other peritoneal fluid colors were more likely to have type B lesions (orange = 55%, pink = 50%, red = 60%; p = 0.02).

As for pelvic pains reported prior to surgery, the patients' acyclic pelvic pain experience differed by peritoneal fluid color (p = 0.001), with those with red peritoneal fluid less likely to have reported acyclic pelvic pain in the past 3 months (57%) compared to those with the other three colors more likely to report acyclic pelvic pain (69%–79%).

In terms of peritoneal fluid volume and hormone use at time of surgery; patients with low volume (≤3 ml) were more likely to be on hormones (89%) compared to those with the highest volume >11 ml (77%) (p = 0.01; Table 3). We did not observe significant differences in type of hormones, menstrual cycle phase, endometriosis macro-phenotype, rASRM stage, or lesion appearance by peritoneal fluid volume. Use of pain medication at time of surgery was suggestively associated with smaller peritoneal fluid volume (23% for ≤6 ml and 13%–16% for >7 ml; p = 0.08). There was a significant difference in peritoneal fluid volume by those with and without superficial endometriosis lesions identified in the posterior cul-de-sac (p = 0.007), with those having greater peritoneal fluid volume (>11 ml) being more likely to have posterior cul-de-sac lesions (97%) compared to those with less volume (≤3 ml, 88%). When assessing the relationship between peritoneal fluid volume and pain symptoms, there were significant differences in peritoneal fluid volume by acyclic pelvic pain experience (p = 0.02). Those with greater volume (>11 ml) were more likely to report acyclic pelvic pain (70%) compared to those with low volume (≤3 ml, 66%), although no linear trend was observed.

Table 3

Table 3. Patient characteristics, endometriosis type, lesion location, and color by peritoneal fluid volume (n = 545).

Discussion

In a sample consisting largely of adolescents and young adults with endometriosis with detailed information on patient characteristics including surgically visualized endometriosis phenotypes, symptoms, and medication exposures, we observed that peritoneal fluid color and volume differed among some characteristics.

Our results showed that menstrual cycle phase and exogenous hormone type used at time of surgery were significantly associated with differences in peritoneal fluid color or volume. Peritoneal fluid is normally present in the pelvic cavity and its volume has been reported to differ corresponding to the menstrual cycle phase in women undergoing laparoscopy (7, 15–17). One study examined peritoneal fluid volume in 158 women who were menstruating and undergoing laparoscopy, and observed an increase in peritoneal fluid volume after ovulation; with 4–9 ml in days 1–13 and 22 ml in day 14–16 of the menstrual cycle (7). This variation in peritoneal fluid volume by the menstrual cycle phase is thought to be due to permeability caused by increasing concentration of estradiol and progesterone (7, 17), although we did not observe clear differences in peritoneal fluid volume by menstrual cycle phase in our study population. Women using hormonal contraceptives (n = 40) has been reported to have lower peritoneal fluid volume (average 5.4 ml) compared to women who were menstruating (7), which was consistent with our finding. While we did not observe any significant associations with use of pain medication or antidepressant/anti-anxiety medications and peritoneal fluid color, our data suggested that those who were on pain medication were more likely to have lower peritoneal fluid volume. This could be because pain medications, mainly anti-inflammatory drugs, could lead to reduced inflammation in the pelvis and lower vascular permeability, resulting in lower peritoneal fluid volume.

Our study population consisted primarily of superficial peritoneal only and rASRM stage I or II disease (both 95%). Therefore, we could not infer clear differences by macro-phentype or rASRM stage. While there was no hint of a gradient in volume, we did observe suggestive differences in peritoneal fluid color by rASRM stage, with pink or red colors being more prevalent in stage III or IV diseases. However, we only had 21 endometriosis patients with rASRM stage III or IV and therefore replication in an independent dataset is needed to confirm this finding. Previous studies reported conflicting results on the association between peritoneal fluid volume and rASRM stage of endometriosis. One small study (n = 58) examined peritoneal fluid volume in women with endometriosis with different rASRM stage and observed that the average peritoneal fluid volume was lower in those with rASRM stage III or IV endometriosis compared to those with rASRM stage I (7). In contrast, another small study (n = 32) reported that those classified as “severe endometriosis” had higher peritoneal fluid volume compared to those classified as “mild or moderate endometriosis” (6). Another study of 272 endometriosis patients, all of whom presented with infertility, reported no correlation between peritoneal fluid color or volume with rASRM stage (5). Further studies are warranted to investigate the association between macro-phenotype and rASRM stage with peritoneal fluid volume.

In our study population, 64% of the patients presented with red peritoneal fluid, which is in line with another study reporting red peritoneal fluid was the most dominant color (88.9%) among patients with endometriosis (5). Red peritoneal fluid likely indicates the presence of blood or hemoglobin. A prior study reported that peritoneal fluid of women with endometriosis has higher concentration of hemoglobin compared to women without, which may suggest a dysfunction of the mechanism responsible for eliminating hemoglobin from the pelvic cavity after retrograde menstruation (18). This study did not observe differences in peritoneal fluid hemoglobin concentration by rASRM stage, although the study was likely to be limited with small sample size (n = 110). To our knowledge, the current study is the first to explore the associations between peritoneal fluid color with lesion appearance. Red lesions have been shown to be associated with more active local inflammation (19, 20). It is not clear why we observed those with red lesions were more likely to have red peritoneal fluid, but it is possible that this may be due to having red lesions reflecting more active local inflammation, promoting neovascularization within the peritoneal environment and increased vascular permeability, resulting in microhemorrhage.

Interestingly, patients with acyclic pelvic pain were less likely to present with red peritoneal fluid and more likely to have higher peritoneal fluid volume. In our data, we did not observe differences in prevalence of hormone use or type used at time of surgery by acyclic pelvic pain status, and therefore it is unlikely that hormone medication is driving these results. Interestingly, those with acyclic pelvic pain have greater volume, which could suggest more local inflammation and vascular permeability (17), although more detailed research investigating peritoneal fluid biomarkers will be needed to further elucidate the potential underlying biology.

To our best knowledge, this is the largest study presenting unique data on descriptive, gross characteristics of the peritoneal fluids in predominantly adolescents and young adults with endometriosis. Another strength of this study is that we were able to associate the peritoneal fluid characteristics with detailed surgical phenotype collected at time of endometriosis-related surgery and patient-reported pelvic pain symptoms. Further research examining biomarkers in these peritoneal fluid samples and their relation to color and volume will advance our knowledge of the differences in the local microenvironment. Since our study population is predominantly a young population mainly presenting with pelvic pain and rASRM stage I disease, these results will not be generalizable to endometriosis patients presenting solely with infertility or with higher disease stage. We acknowledge that for some of the patient characteristics, the sample size was small for some categories and therefore warrant caution for interpretation. Future studies with large samples sizes should explore a wider range of endometriosis phenotypic diversity.

In summary, we investigated differences in gross characteristics of peritoneal fluids by patient characteristics and surgically visualized endometriosis phenotypes, highlighting the importance to account for menstrual cycle phase and exogenous hormonal exposures when designing research using peritoneal fluid samples and conducting studies measuring peritoneal fluid biomarkers to advance our understanding of endometriosis pathophysiology.

Data availability statement

Data are not publicly available due to information that could compromise research participants’ privacy and consent. However, experienced scientists who would like to inquire regarding use of data from this study to address specific hypotheses or replicate the analyses in this study may submit an application and research proposal. Data requests must be reviewed and approved by the Brigham and Women's Hospital Institutional Review Broad (https://www.brighamandwomens.org/research/research-administration). All inquiries should be directed to the A2A cohort leadership committee (womenshealthstudy@bwh.harvard.edu). Data sharing will require a fully executed Data Usage Agreement.

Ethics statement

The studies involving humans were approved by the Boston Children's Hospital (BCH) Institutional Review Board on behalf of both BCH and Brigham and Women's Hospital. Written informed consent was obtained, with both parental consent and participant assent for individuals aged less than 18 years at enrollment. The studies were conducted in accordance with the local legislation and institutional requirements. Written informed consent for participation in this study was provided by the participants’ legal guardians/next of kin.

Author contributions

AY: Writing – original draft, Writing – review & editing, Investigation, Conceptualization. MD: Writing – review & editing, Formal analysis, Methodology. AV: Formal analysis, Methodology, Writing – review & editing, Data curation, Software. HH: Writing – review & editing, Investigation. AS: Investigation, Writing – review & editing. KT: Investigation, Writing – review & editing, Funding acquisition, Resources. SM: Funding acquisition, Investigation, Resources, Writing – review & editing, Conceptualization. NS: Investigation, Writing – review & editing, Conceptualization, Supervision, Writing – original draft, Methodology.

Funding

The author(s) declare financial support was received for the research, authorship, and/or publication of this article.

Financial support for establishment of and data collection within the A2A cohort were provided by the J. Willard and Alice S. Marriott Foundation. AS, KT, SM were supported by NICHD R01 HD94842 and NICHD R21 HD96358. HH, KT, SM. were supported by DoD W81XWH18PRMRPDA. AS, was supported by the Marriott Daughter's Foundation. NS and KT were supported by NICHD R21HD107266.

Acknowledgment

The authors would like to thank the participants of the Women's Health Study: From Adolescence to Adulthood for their valuable contributions and the staff of the Boston Center for Endometriosis.

Conflict of interest

KT and NS receive grant funding from Aspira Women's Health, which is not related to this study. SM reports consultancy for and grant funding from AbbVie for population-based research unrelated to this project and from Frontiers in Reproductive Health as Field Chief Editor.

The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Publisher's note

All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.

Supplementary material

The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/frph.2023.1297907/full#supplementary-material

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