REVIEW article
Front. Genome Ed.
Sec. Genome Editing in Plants
This article is part of the Research TopicThe Expanding Horizon of Genome Editing and Functional Genomics in PlantsView all articles
A long journey towards genome editing technologies in plants: a technical and critical review of genome editing technologies
Provisionally accepted
- BIOS, Centre de Coopération Internationale en Recherche Agronomique pour le Développement, Institut National de la Recherche Agronomique (INRA), Montpellier, France
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Advancements in genome editing technologies, notably CRISPR/Cas9, base editing (BE), and prime editing (PE), have revolutionized plant biotechnology, offering unprecedented precision in crop improvement to address the ongoing global warming challenge. This review provides a critical analysis of recent developments in SpCas9-based editing tools, emphasizing enhancements in editing efficiency and specificity and follow the chronological development of editing tools. We explore methodological innovations, including dual pegRNA strategies and site-specific integrases, that have expanded the potential of PE for precise gene insertions. By integrating insights into DNA repair mechanisms and leveraging SpCas9 enhancements, we outline future directions for the application of genome editing in plant breeding.
Keywords: plant, Genome editing, Prime Editing, SpCas9, knock-in
Received: 10 Jul 2025; Accepted: 29 Oct 2025.
Copyright: © 2025 Gallo, Meunier and Perin. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Christophe Perin, perin@cirad.fr
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