ORIGINAL RESEARCH article
Front. Nephrol.
Sec. Clinical Research in Nephrology
Volume 5 - 2025 | doi: 10.3389/fneph.2025.1601311
Title. DNA Damage and Repair in Patients with Early Chronic Kidney Disease with or without Type 2 Diabetes
Provisionally accepted
Alejandra Guillermina Miranda-Díaz1*
Jorge Andrade Sierra2
Leonardo Pazarín-Villaseñor2
Andrés García-Sánchez3,4
Ernesto German Cardona Muñoz4
Tannia Isabel Campos-Ballardo4Daniel Román-Rojas4Natalia Díaz-de la Cruz4Sylvia Elena Totsuka-Sutto4Wendy Campos-Pérez4
ERIKA MARTINEZ-LOPEZ5Luis Francisco Gómez-Hermosillo2Jorge Casillas-Moreno2
Raquel Echavarria6- 1Department of Physiology, University Center for Health Sciences, University of Guadalajara, Guadalajara, Mexico
- 2Civil Hospital of Guadalajara, Guadalajara, Jalisco, Mexico
- 3University of Guadalajara, Guadalajara, Jalisco, Mexico
- 4Departamento de Fisiología, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara, Jalisco, Mexico, Guadalajara, Jalisco, Mexico
- 5Department of Molecular Biology and Genomics, Institute of Nutrigenetics and Translational Nutrigenomics, University of Guadalajara, Guadalajara, Mexico
- 6Centro de Investigación Biomédica de Occidente (CIBO), Guadalajara, Jalisco, Mexico
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Chronic kidney disease (CKD) may improve with appropriate management and close monitoring to prevent the risk of progression to end-stage kidney disease (ESKD). The present study aimed to determine oxidative damage and DNA repair in early kidney disease in patients with and without type 2 diabetes (T2D). Using ELISA, serum levels of the oxidative DNA damage marker (8OHdG) and the DNA repair marker (hOGG1) were determined in 100 patients with T2D and 88 without T2D in stages 1, 2, and 3 of CKD. The mean number of years of T2D in patients in stages 1, 2, and 3 was 13.93±2.09 years.Significantly increased levels of the 8-OHdG marker were found in stage 3 CKD patients with T2D, 4.96(4.17-5.08) ng/mL vs. 4.13(3.49-4.60) ng/mL without T2D (p=0.006). hOGG1 enzyme levels were significantly decreased in patients with T2D from stage 2, 0.08(0.063-0.082) ng/mL vs. 0.37(0.18-0.36) ng/mL, (p=0.006) and in stage 3 with T2D 0.09(0.08-0.11) ng/mL vs. 0.53(0.07-0.96) ng/mL without T2D (p=0.007). A positive correlation was found between CKD stage and hOGG1 levels in patients with T2D (rho=0.473, p<0.001). 8-OHdG concentration showed an inverse correlation with CKD stage in patients without T2D (rho=-0-274, p=0.030).In conclusion, we found an imbalance of DNA repair enzymes in stages 2 and 3 of CKD in T2D patients and an increase of oxidative DNA damage markers in stage 3 of CKD in T2D patients. Determination of DNA damage and repair markers in the early stages of CKD may facilitate timely diagnosis and treatment of CKD.
Keywords: Oxidative damage to DNA, repair of oxidative damage to DNA, early kidney disease, Chronic Kidney Disease, Diabetes Mellitus
Received: 27 Mar 2025; Accepted: 28 Jun 2025.
Copyright: © 2025 Miranda-Díaz, Andrade Sierra, Pazarín-Villaseñor, García-Sánchez, Cardona Muñoz, Campos-Ballardo, Román-Rojas, Díaz-de la Cruz, Totsuka-Sutto, Campos-Pérez, MARTINEZ-LOPEZ, Gómez-Hermosillo, Casillas-Moreno and Echavarria. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Alejandra Guillermina Miranda-Díaz, Department of Physiology, University Center for Health Sciences, University of Guadalajara, Guadalajara, Mexico
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