Rat retinal function attenuation with IOP elevation is impacted by both blood pressure and intracranial pressure Authors

Sera GanearachchiAnna Kiara van KoeverdenChristine NguyenZheng HeVickie Hoi Ying WongBang Viet Bui^*Da Zhao

• The Department of Optometry and Vision Sciences, The University of Melbourne, Parkville, Victoria, Australia

Introduction: To consider how pressure and intracranial pressure modifies that way that retinal function responds to intraocular pressure elevation in rats.Methods: Six groups of adult Long-Evans rats (n=7-11 eyes/group, total animals 25) were anaesthetised and underwent acute pressure modification. Blood pressure (BP) was measured via a femoral artery cannular and elevated by Angiotensin II infusion into the vein. Intracranial pressure (ICP) was set to 0, 5 or 25 mmHg in 3 separate groups of rats via a cannula in the lateral ventricle. At each ICP (-5, 5 or 25 mmHg) and BP setting (normal or high), IOP was raised from 10 to 90 mmHg in 10 mmHg steps. At each IOP level ganglion retinal function was assessed using the electroretinogram.Results: Compared with normal blood pressure groups, animals with high blood pressure had significantly smaller baseline ganglion cell mediated scotopic threshold responses (STR). Animals with high ICP, had larger amplitudes in the compared with normal and low ICP groups. Both high BP and high ICP rendered retinal function less susceptible to IOP elevation, however the effect was greater for high BP. Conclusion: Retinal function is critically dependent on ocular perfusion pressure; excessive low or high perfusion attenuates function. The ocular perfusion pressure (BP-IOP) relationship largely accounts for the effect of IOP and BP modulation on retinal function but could not account for differences in ganglion cell function between ICP levels.