ORIGINAL RESEARCH article
Front. Physiol.
Sec. Renal Physiology and Pathophysiology
Volume 16 - 2025 | doi: 10.3389/fphys.2025.1683573
This article is part of the Research TopicCardiovascular–Kidney–Metabolic Syndrome: Interorgan Crosstalk, Pathophysiology, and TherapeuticsView all 4 articles
Integration of Transcriptomics and Metabolomics Identifies Pathways in Intermittent Fasting and Renal Injury Induced by High-Fat Diet in Mice
Provisionally accepted
- 1Central China Normal University, Wuhan, China
- 2Tsinghua University, Beijing, China
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Obesity, a worldwide epidemic, is often accompanied by renal dysfunction or accelerating kidney disease. Intermittent fasting (IF) has become a popular weight loss approach but the data for obesity-related kidney disease is very limited. Moreover, there is currently no combined omics study of its related metabolism, mechanisms, and pathways. The purpose of this study was to examine the preventive effect of IF on renal injury induced by high-fat diet (HFD) and to explore the related pathways based on omics analysis. We used HFD to induce obesity-related renal injury. During the IF intervention, the mice were allowed free access to regular chow every other day and were not provided food on the other day. Our data found that IF could effectively prevent obesity-related renal injury in glomerular morphological changes and urine components. Metabolomic and transcriptomic analyses revealed that IF affected the thermogenesis pathway, cholesterol metabolism pathway, and glycerolipid and glycerophospholipid metabolism pathways and prevented and alleviated obesity-related renal injury through inflammation pathways and the insulin resistance pathway. This research would provide valuable data for the prevention and treatment of kidney diseases related to obesity.
Keywords: Obesity, intermittent fasting, renal injury, Metabolomics, Transcriptomics
Received: 11 Aug 2025; Accepted: 30 Sep 2025.
Copyright: © 2025 Yuan, Song, Luan, Hong, Hu, Ding, Zhang and Xiong. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Yingzhe Xiong, xiongyingzhe@126.com
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