Expression Efficiency | List of Frontiers open access articles

Original Research

Published on 18 Dec 2024

A novel approach for efficient co-expression of two foreign genes based on the reverse genetic system of Newcastle disease virus

in Virology

Ting Lan
Qilong Liu
Jinying Ge
Yong Wang

Frontiers in Microbiology

doi 10.3389/fmicb.2024.1442551

3,726 views
1 citation

Review

Published on 28 Mar 2024

Codon-optimization in gene therapy: promises, prospects and challenges

in Synthetic Biology

Anastasiia Iu Paremskaia
Anna A. Kogan
Anastasiia Murashkina
Daria A. Naumova
Anakha Satish
Ivan S. Abramov
Sofya G. Feoktistova
Olga N. Mityaeva
Andrei A. Deviatkin
Pavel Yu Volchkov

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2024.1371596

18,680 views
42 citations

Original Research

Published on 19 Dec 2018

Exploration of an Actin Promoter-Based Transient Expression Vector to Trace the Cellular Localization of Nucleorhabdovirus Proteins in Leafhopper Cultured Cells

in Virology

Xiao-Feng Zhang
Yunjie Xie
Haitao Wang
Juan Wang
Hongyan Chen
Tianbao Zeng
Yibing Zhao
Taiyun Wei

Frontiers in Microbiology

doi 10.3389/fmicb.2018.03034

5,102 views
6 citations

Original Research

Published on 31 May 2016

Isolation and Functional Characterization of Bidirectional Promoters in Rice

in Plant Cell Biology

Rui Wang
Yan Yan
Menglin Zhu
Mei Yang
Fei Zhou
Hao Chen
Yongjun Lin

Frontiers in Plant Science

doi 10.3389/fpls.2016.00766

7,877 views
25 citations

Original Research

Published on 06 Aug 2024

Analysis of plant pararetrovirus promoter sequence(s) for developing a useful synthetic promoter with enhanced activity in rice, pearl millet, and tobacco plants

in Plant Biotechnology

Khushbu Kumari
Tsheten Sherpa
Nrisingha Dey

Frontiers in Plant Science

doi 10.3389/fpls.2024.1426479

3,066 views
6 citations

Review

Accepted on 10 Feb 2026

Keratinases: Microbial Sources, Mechanisms, and Industrial Applications in Waste Valorization

in Microbiotechnology

Yuqing Chu
Shan Chu
Fengna Hu
Mohan Huang
Haoran Lu
Zhiqiao Liu
Fenglian Shan
Xiaotang Chen

Frontiers in Microbiology

104 views

Original Research

Published on 04 Nov 2021

Robust Agrobacterium-Mediated Transient Expression in Two Duckweed Species (Lemnaceae) Directed by Non-replicating, Replicating, and Cell-to-Cell Spreading Vectors

in Bioprocess Engineering

Anton Peterson
Olena Kishchenko
Yuzhen Zhou
Maksym Vasylenko
Anatoli Giritch
Jian Sun
Nikolai Borisjuk
Mykola Kuchuk

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2021.761073

8,412 views
13 citations

Original Research

Published on 01 Sep 2025

Rational design of an epitope-centric vaccine against Pseudomonas aeruginosa using pangenomic insights and immunoinformatics approach

in Vaccines and Molecular Therapeutics

Santhosh Mudipalli Elavarasu
Sasikumar K

Frontiers in Immunology

doi 10.3389/fimmu.2025.1617251

3,288 views
1 citation

Original Research

Published on 29 Nov 2021

The Protocatechuate 3,4-Dioxygenase Solubility (PCDS) Tag Enhances the Expression and Solubility of Heterogenous Proteins in Escherichia coli

in Microbiotechnology

Lei Zou
Sha Li
Nan Li
Shi-Long Ruan
Jing Chen
Jing Wu
Dazhong Yan
Hong-Jun Chao

Frontiers in Microbiology

doi 10.3389/fmicb.2021.779541

4,440 views
5 citations

Original Research

Published on 02 Feb 2023

Design of a multi-epitope vaccine against six Nocardia species based on reverse vaccinology combined with immunoinformatics

in Vaccines and Molecular Therapeutics

Fei Zhu
Caixia Tan
Chunhui Li
Shiyang Ma
Haicheng Wen
Hang Yang
Mingjun Rao
Peipei Zhang
Wenzhong Peng
Yanhui Cui

Frontiers in Immunology

doi 10.3389/fimmu.2023.1100188

13,551 views
28 citations

Original Research

Published on 07 Feb 2024

Differences in molecular characteristics and expression of virulence genes in carbapenem-resistant and sensitive Klebsiella pneumoniae isolates in Ningbo, China

in Antimicrobials, Resistance and Chemotherapy

Min Jiang
Xuedan Qiu
Siyi Shui
Rongqing Zhao
Wenjun Lu
Chenyao Lin
Yanye Tu
Yifeng Wu
Qingcao Li
Qiaoping Wu

Frontiers in Microbiology

doi 10.3389/fmicb.2024.1356229

5,315 views
10 citations

Original Research

Published on 10 Mar 2022

Comparative Analysis of Codon Bias in the Chloroplast Genomes of Theaceae Species

in Genomics of Plants and the Phytoecosystem

Zhanjun Wang
Qianwen Cai
Yue Wang
Minhui Li
Chenchen Wang
Zhaoxia Wang
Chunyan Jiao
Congcong Xu
Hongyan Wang
Zhaoliang Zhang

Frontiers in Genetics

doi 10.3389/fgene.2022.824610

7,025 views
65 citations

Original Research

Published on 06 Apr 2023

Canine adipose tissue-derived MSCs engineered with mRNA to overexpress TSG-6 and enhance the anti-inflammatory effects in canine macrophages

in Veterinary Regenerative Medicine

Ga-Hee Yun
Su-Min Park
Ga-Hyun Lim
Kyoung-Won Seo
Hwa-Young Youn

Frontiers in Veterinary Science

doi 10.3389/fvets.2023.1134185

4,982 views

Original Research

Published on 18 Aug 2025

Construction and optimization of a nisin-controlled expression vector using a pre-screened strong promoter in Streptococcus thermophilus

in Microbiotechnology

Yanxin Ye
Ruiting Zhao
Leilei Li
Zhi Li
Yanyan Chen
Zhenshang Xu

Frontiers in Microbiology

doi 10.3389/fmicb.2025.1586348

2,657 views

Original Research

Published on 02 Jul 2021

Reconstruction of Secondary Metabolic Pathway to Synthesize Novel Metabolite in Saccharopolyspora erythraea

in Industrial Biotechnology

Chong-Yang Ren
Yong Liu
Wen-Ping Wei
Junbiao Dai
Bang-Ce Ye

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2021.628569

5,801 views
6 citations

Articles

A novel approach for efficient co-expression of two foreign genes based on the reverse genetic system of Newcastle disease virus

Codon-optimization in gene therapy: promises, prospects and challenges

Exploration of an Actin Promoter-Based Transient Expression Vector to Trace the Cellular Localization of Nucleorhabdovirus Proteins in Leafhopper Cultured Cells

Isolation and Functional Characterization of Bidirectional Promoters in Rice

Analysis of plant pararetrovirus promoter sequence(s) for developing a useful synthetic promoter with enhanced activity in rice, pearl millet, and tobacco plants

Keratinases: Microbial Sources, Mechanisms, and Industrial Applications in Waste Valorization

Robust Agrobacterium-Mediated Transient Expression in Two Duckweed Species (Lemnaceae) Directed by Non-replicating, Replicating, and Cell-to-Cell Spreading Vectors

Rational design of an epitope-centric vaccine against Pseudomonas aeruginosa using pangenomic insights and immunoinformatics approach

The Protocatechuate 3,4-Dioxygenase Solubility (PCDS) Tag Enhances the Expression and Solubility of Heterogenous Proteins in Escherichia coli

Design of a multi-epitope vaccine against six Nocardia species based on reverse vaccinology combined with immunoinformatics

Differences in molecular characteristics and expression of virulence genes in carbapenem-resistant and sensitive Klebsiella pneumoniae isolates in Ningbo, China

Comparative Analysis of Codon Bias in the Chloroplast Genomes of Theaceae Species

Canine adipose tissue-derived MSCs engineered with mRNA to overexpress TSG-6 and enhance the anti-inflammatory effects in canine macrophages

Construction and optimization of a nisin-controlled expression vector using a pre-screened strong promoter in Streptococcus thermophilus

Reconstruction of Secondary Metabolic Pathway to Synthesize Novel Metabolite in Saccharopolyspora erythraea