Multicellular Spheroids | List of Frontiers open access articles

Original Research

Published on 05 May 2020

Microfluidic Biofabrication of 3D Multicellular Spheroids by Modulation of Non-geometrical Parameters

in Tissue Engineering and Regenerative Medicine

Silvia Lopa
Francesco Piraino
Giuseppe Talò
Valerio Luca Mainardi
Simone Bersini
Margherita Pierro
Luigi Zagra
Marco Rasponi
Matteo Moretti

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2020.00366

6,955 views
17 citations

Original Research

Published on 30 Aug 2022

Surface tension of model tissues during malignant transformation and epithelial–mesenchymal transition

in Cell Adhesion and Migration

Irène Nagle
Alain Richert
Michael Quinteros
Sébastien Janel
Edgar Buysschaert
Nathalie Luciani
Henry Debost
Véronique Thevenet
Claire Wilhelm
Céline Prunier

Frontiers in Cell and Developmental Biology

doi 10.3389/fcell.2022.926322

7,151 views
25 citations

Brief Research Report

Published on 15 Apr 2021

3D Printed Monolithic Device for the Microfluidic Capture, Perfusion, and Analysis of Multicellular Spheroids

in Pharmaceutical Innovation

Alex Markoski
Ian Y. Wong
Jeffrey T. Borenstein

Frontiers in Medical Technology

doi 10.3389/fmedt.2021.646441

5,703 views
7 citations

Original Research

Published on 11 Apr 2024

Deep learning-assisted concentration gradient generation for the study of 3D cell cultures in hydrogel beads of varying stiffness

in Biofabrication

Vasileios Anagnostidis
Anuj Tiwari
Fabrice Gielen

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2024.1364553

2,791 views
4 citations

Review

Published on 24 Jun 2020

Exploiting CRISPR Cas9 in Three-Dimensional Stem Cell Cultures to Model Disease

in Biomaterials

Sneha Gopal
André Lopes Rodrigues
Jonathan S. Dordick

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2020.00692

23,520 views
35 citations

Review

Published on 06 Jan 2026

Construction and application of pancreatic exocrine organoid and spheroid for drug screening and precision medicine

in Cancer Cell Biology

Xin Tan
Bangwei Huang
Xinyi Yang
Pengyuan Wang
Lianghao Hu

Frontiers in Cell and Developmental Biology

doi 10.3389/fcell.2025.1746622

913 views

Original Research

Published on 09 Jun 2022

Novel Brain-Stiffness-Mimicking Matrix Gel Enables Comprehensive Invasion Analysis of 3D Cultured GBM Cells

in Molecular Diagnostics and Therapeutics

Shuowen Wang
Yiqi Wang
Jin Xiong
Wendai Bao
Yaqi Li
Jun Qin
Guang Han
Sheng Hu
Junrong Lei
Zehao Yang

Frontiers in Molecular Biosciences

doi 10.3389/fmolb.2022.885806

5,304 views
8 citations

Original Research

Published on 22 Apr 2022

Intrinsic Differences in Spatiotemporal Organization and Stromal Cell Interactions Between Isogenic Lung Cancer Cells of Epithelial and Mesenchymal Phenotypes Revealed by High-Dimensional Single-Cell Analysis of Heterotypic 3D Spheroid Models

in Cancer Immunity and Immunotherapy

Maria L. Lotsberg
Gro V. Røsland
Austin J. Rayford
Sissel E. Dyrstad
Camilla T. Ekanger
Ning Lu
Kirstine Frantz
Linda E. B. Stuhr
Henrik J. Ditzel
Jean Paul Thiery

Frontiers in Oncology

doi 10.3389/fonc.2022.818437

9,936 views
18 citations

Review

Published on 25 Feb 2016

Differential Superiority of Heavy Charged-Particle Irradiation to X-Rays: Studies on Biological Effectiveness and Side Effect Mechanisms in Multicellular Tumor and Normal Tissue Models

in Radiation Oncology

Stefan Walenta
Wolfgang Mueller-Klieser

Frontiers in Oncology

doi 10.3389/fonc.2016.00030

8,104 views
26 citations

Original Research

Published on 13 Feb 2026

7α-acetoxy-6β-hydroxyroyleanone (Roy) modulates IL-6/STAT3/JAK2 mRNA expression and suppresses tumor growth in glioblastoma cell models

in Ethnopharmacology

Mariana Magalhães
Renato Spigarelli
Eva María Domínguez-Martín
Lino Ferreira
Thomas Efferth
Patrícia Rijo
Enzo Spisni
Célia Cabral

Frontiers in Pharmacology

doi 10.3389/fphar.2026.1728792

514 views

Original Research

Published on 03 Nov 2022

Novel kinome profiling technology reveals drug treatment is patient and 2D/3D model dependent in glioblastoma

in Cancer Molecular Targets and Therapeutics

Federica Fabro
Nynke M. Kannegieter
Erik L. de Graaf
Karla Queiroz
Martine L. M. Lamfers
Anna Ressa
Sieger Leenstra

Frontiers in Oncology

doi 10.3389/fonc.2022.1012236

3,667 views
3 citations

Original Research

Published on 24 Jun 2021

A Novel Ruthenium(II) Complex With Lapachol Induces G2/M Phase Arrest Through Aurora-B Kinase Down-Regulation and ROS-Mediated Apoptosis in Human Prostate Adenocarcinoma Cells

in Pharmacology of Anti-Cancer Drugs

Rone A. De Grandis
Katia M. Oliveira
Adriana P. M. Guedes
Patrick W. S. dos Santos
Alexandre F. Aissa
Alzir A. Batista
Fernando R. Pavan

Frontiers in Oncology

doi 10.3389/fonc.2021.682968

5,331 views
23 citations

Original Research

Published on 09 Jul 2019

Ruthenium Complexes Containing Heterocyclic Thioamidates Trigger Caspase-Mediated Apoptosis Through MAPK Signaling in Human Hepatocellular Carcinoma Cells

in Cancer Molecular Targets and Therapeutics

Sara P. Neves
Nanashara C. de Carvalho
Monize M. da Silva
Ana Carolina B. C. Rodrigues
Larissa M. Bomfim
Rosane B. Dias
Caroline B. S. Sales
Clarissa A. Gurgel Rocha
Milena B. P. Soares
Alzir A. Batista

Frontiers in Oncology

doi 10.3389/fonc.2019.00562

5,397 views
23 citations

Original Research

Published on 17 Feb 2022

Three-Dimensional Growth of Prostate Cancer Cells Exposed to Simulated Microgravity

in Cell Growth and Division

Dorothea Dietrichs
Daniela Grimm
Jayashree Sahana
Daniela Melnik
Thomas J. Corydon
Markus Wehland
Marcus Krüger
Randy Vermeesen
Bjorn Baselet
Sarah Baatout

Frontiers in Cell and Developmental Biology

doi 10.3389/fcell.2022.841017

7,703 views
28 citations

Original Research

Published on 30 Jun 2021

Alterations of Growth and Focal Adhesion Molecules in Human Breast Cancer Cells Exposed to the Random Positioning Machine

in Cell Adhesion and Migration

Jayashree Sahana
Thomas J. Corydon
Markus Wehland
Marcus Krüger
Sascha Kopp
Daniela Melnik
Stefan Kahlert
Borna Relja
Manfred Infanger
Daniela Grimm

Frontiers in Cell and Developmental Biology

doi 10.3389/fcell.2021.672098

8,956 views
26 citations

Articles

Microfluidic Biofabrication of 3D Multicellular Spheroids by Modulation of Non-geometrical Parameters

Surface tension of model tissues during malignant transformation and epithelial–mesenchymal transition

3D Printed Monolithic Device for the Microfluidic Capture, Perfusion, and Analysis of Multicellular Spheroids

Deep learning-assisted concentration gradient generation for the study of 3D cell cultures in hydrogel beads of varying stiffness

Exploiting CRISPR Cas9 in Three-Dimensional Stem Cell Cultures to Model Disease

Construction and application of pancreatic exocrine organoid and spheroid for drug screening and precision medicine

Novel Brain-Stiffness-Mimicking Matrix Gel Enables Comprehensive Invasion Analysis of 3D Cultured GBM Cells

Intrinsic Differences in Spatiotemporal Organization and Stromal Cell Interactions Between Isogenic Lung Cancer Cells of Epithelial and Mesenchymal Phenotypes Revealed by High-Dimensional Single-Cell Analysis of Heterotypic 3D Spheroid Models

Differential Superiority of Heavy Charged-Particle Irradiation to X-Rays: Studies on Biological Effectiveness and Side Effect Mechanisms in Multicellular Tumor and Normal Tissue Models

7α-acetoxy-6β-hydroxyroyleanone (Roy) modulates IL-6/STAT3/JAK2 mRNA expression and suppresses tumor growth in glioblastoma cell models

Novel kinome profiling technology reveals drug treatment is patient and 2D/3D model dependent in glioblastoma

A Novel Ruthenium(II) Complex With Lapachol Induces G2/M Phase Arrest Through Aurora-B Kinase Down-Regulation and ROS-Mediated Apoptosis in Human Prostate Adenocarcinoma Cells

Ruthenium Complexes Containing Heterocyclic Thioamidates Trigger Caspase-Mediated Apoptosis Through MAPK Signaling in Human Hepatocellular Carcinoma Cells

Three-Dimensional Growth of Prostate Cancer Cells Exposed to Simulated Microgravity

Alterations of Growth and Focal Adhesion Molecules in Human Breast Cancer Cells Exposed to the Random Positioning Machine