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Front. Bioeng. Biotechnol. | doi: 10.3389/fbioe.2019.00355

Stimulation of Osteogenic Differentiation of Induced Pluripotent Stem Cells (iPSCs) Using Bioactive Glasses: An in vitro Study

 Saeid Kargozar1, 2*,  Nasrin Lotfibakhshaeish3, Somaye Ebrahimi Barough3, Bahareh Nazari3 and  Robert G. Hill4
  • 1Mashhad University of Medical Sciences, Iran
  • 2Department of Anatomy & Cell Biology, Faculty of Medicine, Mashhad university of Medical Sciences, Iran
  • 3Department of Tissue Engineering and Applied Cellular Sciences, Faculty of Medical Technology, Tehran University of Medical Sciences, Iran
  • 4Barts and The London School of Medicine and Dentistry, Queen Mary University of London, United Kingdom

Selection and use of an optimal cell source for bone tissue engineering remain a challenging issue; the invention of induced pluripotent stem cells (iPSCs) have created new hopes on this regard. At the present study, we attempted to show the usability of iPSCs in combination with bioactive glasses (BGs) for bone regeneration applications. For this aim, iPSCs were cultured and incubated with the strontium and cobalt-containing BGs for different intervals (1, 5, and 7 days). The cell cytotoxicity and attachment were assessed using MTT assay and scanning electron microscopy (SEM), respectively. Moreover, the osteogenic differentiation of iPSCs seeded onto the glasses was evaluated using alkaline phosphatase (ALP) activity assay and real-time PCR. The obtained results clarified that although the cell viability is decreased during a 7 day period, the iPSCs could adhere and expand onto the BGs particles and over-express the osteogenic markers, including osteocalcin, osteonectin, and Runx2. Based on the data, we conclude that iPSCs in a combination of BGs can be considered as a potential candidate for bone tissue engineering strategies.

Keywords: bone tissue engineering, Induced Pluripotent Stem Cells, Bioactive glass, in vitro., Osteogenesis

Received: 08 Feb 2019; Accepted: 08 Nov 2019.

Copyright: © 2019 Kargozar, Lotfibakhshaeish, Ebrahimi Barough, Nazari and Hill. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Dr. Saeid Kargozar, Mashhad University of Medical Sciences, Mashhad, Iran,