In the original article, there were various errors present throughout the main text. These errors have been corrected in the original article.
Additionally, Figure 1 and Table 1 have been updated. The updated figure and table are shown below. The Funding statement has also been updated and is shown below.
FIGURE 1
TABLE 1
| Diagnostic PCR(s) | gRNA | |
|---|---|---|
| GWD—5′ exon 1 | GWD Forward primer 1 | gJ: TCAGTGGTAAGTACAGCATG |
| 5′ TTTGTATTGACTGATTTTGTATTGT 3′ | gK: AGGGAATAACTTGCTGTACC | |
| GWD Reverse primer 1 | gL: GTTTCGAGGTAACAGGTTAA | |
| FAM 5′ TAGTTTCTAAGCCCCAAGCA3′ | gM: GTACAGCAAGTTATTCCCTA | |
| GWD—3′ exon 24 + 25 | GWD Forward primer 2: | gA: GGAGAGGAGGAAATTCCTGA |
| 5′ TCAGTCCAGTTGAAGCCGTTG 3′ | gB: TGTTCGAGCTAGAAATGGGA | |
| GWD Reverse primer 2: | gC: GCTGACCTCCAAGCAAAGGA | |
| FAM 5′ TCACGAGTTCATTCATCTTTCCCA 3′ | gD: ATTGGCTGACCTCCAAGCAA | |
| gE: TTTCTGTTCGAGCTAGAAAT | ||
| gI: CACAACGACAACATATCCAA | ||
| DMR6—5′ exon 1 | DMR6 Forward primer 1 | g43: TTTGAGGGAGAGTAGAGTGG |
| FAM 5′ CCATGGAAACGAAAGTTATTTC 3′ | g44: GTGGCCTATCGGATTCGGGT | |
| DMR6 Reverse primer primer 1 | ||
| 5′ CAACCTAAGTCAATTATTGGAAC 3′ | ||
| DMR6—5′ exon 2 | DMR6 Forward primer 2 | g45: TGGAGAAATATGCTCCTGAA |
| 5′ AGCTGACCGGCAGCAAAATTGGGTAGCTGGGGAATTTTTCA 3′ | ||
| DMR6 Reverse primer 2 | ||
| 5′ GGTTACCATGCATAACTATACACAC 3′ | ||
| FAM primer | ||
| FAM 5′ AGCTGACCGGCAGCAAAATTG 3′ | ||
| DMR6—5′ exon 1 + 2 | DMR6 Forward primer 1 | g43: TTTGAGGGAGAGTAGAGTGG |
| FAM 5′ CCATGGAAACGAAAGTTATTTC′3 | g44: GTGGCCTATCGGATTCGGGT | |
| DMR6 Reverse primer 2 | g45: TGGAGAAATATGCTCCTGAA | |
| 5′ GGTTACCATGCATAACTATACACAC 3′ | ||
| DMR6 Reverse primer 4 | ||
| FAM 5′ CGATGGATTAGAAGGCCATTC 3′ | ||
| DMR6—3′ exon 3 | DMR6 Forward primer primer 3 | g46: GAAGCCATAGCAGAGAGCCT |
| 5′ ATCGTGAGCAGATATTGCACG 3′ | g47: GAATTTGGATCAGTATGGGC | |
| DMR6 Reverse primer 3 | g48: ATCACCAAGATTAATGACAA | |
| FAM 5′ GGTTTACCTGCAATTGATCAC 3′ |
gRNAs and diagnostic IDAA primers for each of the four target regions. Scores and first selection of gRNAs were obtained by feeding ca 1 kb regions to the in silico prediction servers CHOPCHOP (http://chopchop.cbu.uib.no/), CRISPRater (https://crispr.cos.uniheidelberg.de/) and SSC (http://crispr.dfci.harvard.edu/SSC/).
Statements
Funding
Kartoffelafgiftfonden (2021) “Samtidig introduktion af flere egenskaber i kartoffel vha. DNA-fri CRISPR”. Kartoffelafgiftfonden (2022)” Reduceret miljø og klima aftryk gennem præcis forædling af skimmel resistens i stivelses kartofler”. Novo Nordisk Foundation (2020) “Gene edited potatoes for reduced pesticide usage” (NNF19OC0057208). Grønt Udviklings-og Demonstrations program (GUDP) (2020) “KRISPS Kartofler med Resistens og Innovativ Stivelse som Platform for Synergi mellem grøn og økonomisk bæredygtighed” (34009-19-1531). DFF/Independent Research Fund Denmark (2022) “Green CRISPR production platform for new starch qualities” (1032-00399B).”
The authors apologize for these errors and state that they do not change the scientific conclusions of the article in any way. The original article has been updated.
Publisher’s note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.
Summary
Keywords
ribonucleoprotein/gRNA design, gene editing (CRISPR/Cas9), protoplast, gRNA efficiency, multiplexing, complex genome, potato
Citation
Carlsen FM, Johansen IE, Yang Z, Liu Y, Westberg IN, Kieu NP, Jørgensen B, Lenman M, Andreasson E, Nielsen KL, Blennow A and Petersen BL (2022) Corrigendum: Strategies for Efficient Gene Editing in Protoplasts of Solanum tuberosum Theme: Determining gRNA Efficiency Design by Utilizing Protoplast (Research). Front. Genome Ed. 4:914100. doi: 10.3389/fgeed.2022.914100
Received
06 April 2022
Accepted
21 June 2022
Published
04 August 2022
Volume
4 - 2022
Reviewed by
Edited andLeena Tripathi, International Institute of Tropical Agriculture (IITA), Kenya
Updates
Copyright
© 2022 Carlsen, Johansen, Yang, Liu, Westberg, Kieu, Jørgensen, Lenman, Andreasson, Nielsen, Blennow and Petersen.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Bent Larsen Petersen, blp@plen.ku.dk
†These authors have contributed equally to this work
This article was submitted to Genome Editing in Plants, a section of the journal Frontiers in Genome Editing
Disclaimer
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.