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General Commentary ARTICLE

Front. Genet., 05 November 2013 | https://doi.org/10.3389/fgene.2013.00225

The curse of the missing heritability

  • Division of Computational Genetics, Department of Clinical Sciences, Swedish University of Agricultural Sciences, Uppsala, Sweden

A commentary on
Finding the sources of missing heritability in a yeast cross

by Bloom, J. S., Ehrenreich, I. M., Loo, W. T., Lite, T. L., and Kruglyak, L. (2013). Nature 494, 234–237. doi: 10.1038/nature11867

Since “the case of the missing heritability” was highlighted 5 years ago (Maher, 2008), scientists have been investigating various possible explanations for this issue (Manolio et al., 2009; Slatkin, 2009; Eichler et al., 2010; Zuk et al., 2012). Recently, Bloom et al. (2013) conducted a linkage analysis in a large yeast Saccharomyces cerevisiae cross with high statistical power to map functional quantitative trait loci (QTL) and found that nearly all the additive genetic contribution can be explained by the detected QTL. It is striking that the “old-fashioned” linkage analysis can resolve the missing heritability problem arisen in the high-throughput genome-wide association study (GWAS) era. Compared to human population studies, an intercross creates large linkage disequilibrium (LD) blocks that greatly enhance statistical power but also reduce QTL mapping resolution. Simple simulations (Figure 1) indicate that the real sources or architecture of missing heritability will remain undiscovered due to LD. Breaking down LD would provide better resolution but reduce the power. This commentary is raised to emphasize the trade-off between resolution and statistical power in mapping functional loci.

FIGURE 1
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Figure 1. Information captured by randomly selected markers in the yeast cross6. (A) Proportion of variance explained in the caffeine phenotype by different numbers of randomly selected markers across the genome. Hundred times of random sampling were replicated for each value on the x-axis. The thick and thin horizontal dashed lines indicate Bloom et al.'s6 estimates of the total narrow sense heritability (h2) and the h2 explained by their detected QTL. (B) Comparison of the elements in the genomic kinship matrix (G) and those in the kinship matrix estimated by 32 randomly selected markers (R) in the yeast cross. Two markers were randomly selected from each of the 16 yeast chromosomes. G = ZZT/n, R = XXT/m, where n is the number of markers across the genome (11,623), m is the number of randomly selected markers (32), Z is an N (number of individuals)-by-n matrix of genotype data and X is an N-by-m matrix for the selected markers. The straight line indicates equality and is shown as a visual reference.

Linkage analysis or QTL interval mapping in an experimental design is a classic method in quantitative genetics to detect QTL, which allows inferring QTL effects in an un-typed chromosomal interval harbored by flanking genetic markers (Lynch and Walsh, 1998). In an F2 cross, the observed LD blocks are often very large, due to limited number of recombination events happened in the F1 individuals, though the recombination rate in yeast is relatively high. For example, among the detected QTL for yeast growth in E6 berbamine (Figure 3 in Bloom et al., 2013), the two QTL on chromosome 1 covered the two clear LD blocks (not shown) on the chromosome, and the QTL on chromosome 9 covered most of the chromosome. The finding that the detected QTL can explain almost all the narrow sense heritability (h2) is expected given that the kinship estimates using only the significant QTL are similar to the genomic kinship. Even a small number of randomly selected markers can resemble the genomic kinship and give similar heritability estimates (Figure 1B), because the number of LD blocks in the entire genome is limited. The prediction of trait values using detected QTL was good according to cross validation, because the specific F2 population share similar LD patterns, but such prediction would not perform as superior in another population with different LD pattern. Related empirical evidence can be seen in human height (Makowsky et al., 2011) and marker-assisted selection (Dekkers, 2004), where detected QTL were unsuccessful for out-sample prediction purposes.

If a future generation (e.g., F8) with small LD blocks is developed from the F2, the statistical power for mapping QTL will decrease. One reason is that a single-locus test for QTL within a large LD block is very likely boosted by multiple QTL within the LD block whose effects are much smaller. The single QTL effect can be simply a combined effect of multiple QTL, and its standard error is underestimated without considering the linkage with other QTL in the same LD region. Assume that there are two functional SNPs x1 and x2 in a chromosomal region with high LD, and the phenotype y is determined by y = x1β1 + x2 β 2 + e (1), where β1 and β2 are the effects of the two SNPs; y, x1, and x2 are column vectors of data; e is a vector of residuals. Due to the high LD, x1x2 if x1 and x2 are on the same scale, so that yx11 + β2) + e. In a regression model on the single SNP x1, y = x1β + e (2), the estimated effect for β will be approximately β1 + β2, i.e., a combined effect of both variants. Comparing regression models (1) and (2), the standard error (s.e.) of the estimated β is an underestimate of the s.e. of β 1. This is because the s.e. of β1 is inversely proportional to 1r2 where r is the correlation coefficient between x1 and x2, which is close to 1 due to the high LD, therefore the s.e. of β1 becomes much larger than that of β. When the large LD blocks are broken down, such a combined effect will substantially decrease, leading to lack of statistical power for mapping multiple QTL in the original large LD blocks. One previous empirical example was found in chicken advanced intercross lines (AIL), where only five out of nine QTL detected in the F2 were confirmed by the AIL (Besnier et al., 2011).

Bloom et al.'s study clearly shows that nearly all the h2 in yeast is written in the DNA, which improves our understanding of missing heritability though some resolution is sacrificed. Researchers are searching for genetic architecture that answers not only where but also what and how the sources contribute to the heritability. However, the curse of missing heritability forces us to choose between resolution and power. For many complex traits, such as human height (Yang et al., 2011), their polygenic nature makes it extremely difficult to fine-map even the major contribution of the heritability. In future studies, it is important to check the prediction performance in a validation population, in order to show the real sources of missing heritability. Also, biological information and useful tools other than statistical methods need to be developed and utilized.

Acknowledgments

Xia Shen is funded by a Future Research Leaders grant from Swedish Foundation for Strategic Research (SSF) to Prof. Örjan Carlborg.

References

Besnier, F., Wahlberg, P., Rönnegård, L., Ek, W., Andersson, L., Siegel, P. B., et al. (2011). Fine mapping and replication of QTL in outbred chicken advanced intercross lines. Genet. Sel. Evol. 43, 3. doi: 10.1186/1297-9686-43-3

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Bloom, J. S., Ehrenreich, I. M., Loo, W. T., Lite, T. L., and Kruglyak, L. (2013). Finding the sources of missing heritability in a yeast cross. Nature 494, 234–237. doi: 10.1038/nature11867

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Dekkers, J. C. M. (2004). Commercial application of marker- and gene-assisted selection in livestock: Strategies and lessons. J. Anim. Sci. 82, E313–E328.

Pubmed Abstract | Pubmed Full Text

Eichler, E. E., Flint, J., Gibson, G., Kong, A., Leal, S. M., Moore, J. H., et al. (2010). Missing heritability and strategies for finding the underlying causes of complex disease. Nature Rev. Genet. 11, 446–450. doi: 10.1038/nrg2809

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Lynch, M., and Walsh, B. (1998). Genetics and Analysis of Quantitative Traits. 1 Edn. Sunderland, MA: Sinauer Associates, Inc.

Maher, B. (2008). The case of the missing heritability. Nature 456, 18–21. doi: 10.1038/456018a

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Makowsky, R., Pajewski, N. M., Klimentidis, Y. C., Vazquez, A. I., Duarte, C. W., Allison, D. B., et al. (2011). Beyond missing heritability: prediction of complex traits. PLoS Genet. 7: e1002051. doi: 10.1371/journal.pgen.1002051

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Manolio, T. A., Collins, F. S., Cox, N. J., and Goldstein, D. B. (2009). Finding the missing heritability of complex diseases. Nature 461, 747–753. doi: 10.1038/nature08494

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Slatkin, M. (2009). Epigenetic inheritance and the missing heritability problem. Genetics 182, 845–850. doi: 10.1534/genetics.109.102798

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Yang, J., Manolio, T. A., Pasquale, L. R., Boerwinkle, E., Caporaso, N., Cunningham, J. M., et al. (2011). Genome partitioning of genetic variation for complex traits using common SNPs. Nat. Genet. 43, 519–525. doi: 10.1038/ng.823

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Zuk, O., Hechter, E., Sunyaev, S. R., and Lander, E. S. (2012). The mystery of missing heritability: genetic interactions create phantom heritability. Proc. Natl. Acad. Sci. U.S.A. 109, 1193–1198. doi: 10.1073/pnas.1119675109

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Keywords: missing heritability, quantitative trait loci, intercross, linkage analysis, genomic kinship

Citation: Shen X (2013) The curse of the missing heritability. Front. Genet. 4:225. doi: 10.3389/fgene.2013.00225

Received: 19 July 2013; Accepted: 16 October 2013;
Published online: 05 November 2013.

Edited by:

Frank Emmert-Streib, Queen's University Belfast, UK

Reviewed by:

Gaurav Sablok, Istituto Agrario San Michele, Italy
Zhixiang Lu, University of California, Los Angeles, USA
Pavlos Pavlidis, Heidelberg Institute of Theoretical Studies, Germany

Copyright © 2013 Shen. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

*Correspondence: xia.shen@slu.se