Original Research ARTICLE
Modelization of the regulation of protein synthesis following fertilization in sea urchin shows requirement of two processes: a destabilization of eIF4E:4E-BP complex and a great stimulation of the 4E-BP-degradation mechanism, both rapamycin-sensitive
- 1Ifremer, UMR6197, Laboratoire de Microbiologie des Environnements Extrêmes, Plouzané, France
- 2Université de Nice-Sophia Antipolis, UMR 7271, Laboratoire I3S, Sophia, Antipolis, France
- 3Sorbonne Universités, UPMC University Paris 06, UMR 8227, Integrative Biology of Marine Models, Translation Cell Cycle and Development, Station Biologique de Roscoff, Roscoff cedex, France
- 4CNRS, UMR 8227, Integrative Biology of Marine Models, Translation Cell Cycle and Development, Station Biologique de Roscoff, Roscoff cedex, France
- 5CNRS UMR 6241, Laboratoire LINA, Université de Nantes, Nantes, France
- 6CNRS, IRISA-UMR 6074, Campus de Beaulieu, Rennes, France
- 7INRIA, Centre Rennes – Bretagne Atlantique, Symbiose, Campus de Beaulieu, Rennes, France
Fertilization of sea urchin eggs involves an increase in protein synthesis associated with a decrease in the amount of the translation initiation inhibitor 4E-BP. A highly simple reaction model for the regulation of protein synthesis was built and was used to simulate the physiological changes in the total 4E-BP amount observed during time after fertilization. Our study evidenced that two changes occurring at fertilization are necessary to fit with experimental data. The first change was an 8-fold increase in the dissociation parameter (koff1) of the eIF4E:4E-BP complex. The second was an important 32.5-fold activation of the degradation mechanism of the protein 4E-BP. Additionally, the changes in both processes should occur in 5 min time interval post-fertilization. To validate the model, we checked that the kinetic of the predicted 4.2-fold increase of eIF4E:eIF4G complex concentration at fertilization matched the increase of protein synthesis experimentally observed after fertilization (6.6-fold, SD = 2.3, n = 8). The minimal model was also used to simulate changes observed after fertilization in the presence of rapamycin, a FRAP/mTOR inhibitor. The model showed that the eIF4E:4E-BP complex destabilization was impacted and surprisingly, that the mechanism of 4E-BP degradation was also strongly affected, therefore suggesting that both processes are controlled by the protein kinase FRAP/mTOR.
Early development of the sea urchin embryo is one among the models which contribute to establish the paradigms of the mechanisms of translation, the eukaryotic universal process for protein biosynthesis (Mathews et al., 2007). Many factors are involved in all steps of translation machinery, namely initiation, elongation, and termination and have been extensively reviewed (Mathews et al., 2007). For the great majority of the mRNAs, the initiation step of translation occurs on activated mRNAs that contain a m7GpppN molecule (where N is any of the four nucleotides) at the 5' end, also known as a cap structure, thus referred as cap-dependent translation initiation (Jackson et al., 1995). Not exhaustively, protein translation initiation begins with the binding of the eukaryotic initiation factor 4E (eIF4E) to the cap-structure of the mRNA. The protein eIF4E recruits a large scaffolding protein called eIF4G that interacts, among others, with eIF4A and eIF3 linking the 5' end of the mRNA and the 43S preinitiation complex (Gingras et al., 1999a). After scanning from the 5' end to the start codon, the first amino acid is incorporated and the peptide elongation step proceeds (Jackson et al., 2010, 2012; Hinnebusch, 2011). The protein eIF4E is a major target for regulation of translation initiation (Sonenberg and Gingras, 1998). Its availability in the cells depends on the presence of 4E-BPs, eIF4E binding proteins, which compete with eIF4G for a common binding site on eIF4E (Mader et al., 1995). Thus 4E-BPs sequester eIF4E, and consequently prevent cap-dependent translation (Haghighat et al., 1995). The 4E-BPs phosphorylation status regulates their interaction with eIF4E: underphosphorylated 4E-BPs bind to eIF4E and inhibit cap-dependent translation, whereas hyperphosphorylated forms do not (Pause et al., 1994). Most of the data available to date indicate that FRAP/mTOR (FKBP12 and Rapamycin-Associated Protein/mammalian Target Of Rapamycin) is the main kinase that phosphorylates 4E-BP (Brunn et al., 1997; Burnett et al., 1998) on four conserved residues in metazoans (Gingras et al., 1999b).
Among the experimental advantages of the sea urchin embryo model, genes encoding all factors involved in translation are present and are non-redundant; thus, while mammalian genomes contain three 4E-BP homologs, sea urchin contains an unique form (Morales et al., 2006). At fertilization, an increase in protein synthesis occurs, which is necessary for the initiation of development and for the occurrence of the first cell cycle (Epel, 1990; Cormier et al., 2003; Gilbert, 2003). The protein synthesis increase is independent of new transcription and involves cap-dependent initiation from maternal mRNAs already present in the unfertilized egg (Cormier et al., 2003). Fertilization triggers 4E-BP release from eIF4E and consequently protein synthesis translation through a rapamycin-, and thus, mTOR-sensitive pathway (Cormier et al., 2001; Salaun et al., 2003, 2004). An original mechanism of 4E-BP regulation first demonstrated in sea urchin, corresponds to the rapid disappearance of 4E-BP pool following fertilization (Salaun et al., 2003, 2004, 2005; Oulhen et al., 2007, 2009). Although fertilization is known to provoke many changes in the egg metabolism that could interfere on translation regulation (Epel, 1990; Gilbert, 2003; Parrington et al., 2007), we demonstrate here that a reduced model involving 4E-BP, eIF4E, and eIF4G is sufficient to explain the changes observed at fertilization on 4E-BP level and on the increase of cap-dependent translation. Simulations using this minimal model suggest that both a 32.5-fold increase of the 4E-BP degradation mechanism and an 8-fold change in the dissociation constant (koff1) of eIF4E:4E-BP complex are required at fertilization and should occur in a 5 min time interval after fertilization. Furthermore, simulations of changes after fertilization in the presence of rapamycin show that the drug strongly affects the eIF4E:4E-BP complex stability and, unexpectedly, the 4E-BP degradation mechanism implying that both mechanisms are controlled by the FRAP/mTOR protein kinase.
Materials and Methods
Protein Production and Purification
The construction of the plasmid pAr encoding eIF4E1 from Mus musculus has already been described (Pyronnet et al., 1999) and the protocol to produce and purify Flag-tagged MmIF4E1 was performed as described herein. The cloning of Strongylocentrotus purpuratus 4E-BP and the production of the recombinant His-tagged Sp4E-BP protein was performed as described in Gosselin et al. (2011). Briefly, recombinant Sp4E-BP was produced in BL21 (DE3) strain E.coli (Novagen) and purified on an affinity column, packed with a chelating sepharose Fast Flow Resin preloaded with Ni2+ ions (Amersham Pharmacia Biotech). A second purification step was performed on a Superdex 75 column (Amersham Pharmacia Biotech). After concentration with Amicon® Ultra-4 Centrifugal Filter Units (10 kDa), concentration of the purified proteins was estimated with a Nanodrop ND-1000.
Purification of native eIF4E was performed from Sphaerechinus granularis egg extracts using m7GTP beads as described previously (Oulhen et al., 2010). After washing, beads were suspended in Laemmli loading buffer. The amount of recovered SgIF4E was determined after resolution on SDS-PAGE electrophoresis and revelation by Coomassie blue staining. Quantification was performed by comparison with a range of BSA concentrations migrated on the same gel. The bands were quantified using the ImageJ 1.43j program (Wayne Rasband, National Institutes of Health, USA) after digitization of the stained gel.
Preparation of Gametes and Fertilization
Sphaerechinus granularis sea urchins collected in the Brest area (France) were maintained in running seawater. Spawning of gametes, fertilization, and cell culture were as described (Oulhen et al., 2010). When indicated, 20 μM rapamycin (LC laboratories) from a 20 mM stock solution in ethanol was added to the eggs 20 min before fertilization. Cleavage was scored under a light microscope. Each experiment used gametes from a single female exhibiting greater than 90% fertilization.
Quantification of 4E-BP and eIF4E in Sea Urchin Eggs
To quantify eIF4E and 4E-BP in the sea urchin unfertilized eggs, total crude protein extracts were prepared by direct dissolution of 20 μl egg pellet (corresponding to 12,000 eggs) with 150 μl of SDS-Fix buffer as described (Belle et al., 2011). A range of different extract volumes was submitted to SDS-PAGE and Western-blotted together with known amounts of purified SgIF4E or recombinant Sp4E-BP as prepared above. Proteins in the different samples were immuno-revealed using mouse monoclonal antibody directed against eIF4E from rabbit (BD transduction laboratories, Lexington, KY) or rabbit polyclonal antibodies directed against human 4E-BP2 (Rousseau et al., 1996), a generous gift from Nahum Sonenberg (McGill University, Montreal, Quebec, Canada). The antigen-antibody complex was measured by chemiluminescence using horseradish peroxydase-coupled secondary antibodies according to the manufacturer's instructions (ECL or ECL+, GE Healthcare Life Sciences). Quantification of the bands was done using the ImageJ 1.43j program. Measurement has been performed on extracts from eggs of 9 different females for eIF4E and 4 females for 4E-BP. Protein concentrations were calculated considering an egg volume of 0.3 nl (diameter 80 μM).
Determination of Protein Synthesis in vivo
Unfertilized eggs (5% suspension in sea water) were incubated in the presence of [35S]- L- methionine during 1 h at the final concentration of 10 μCi/ml. When indicated, 20 μM rapamycin was added to the incubation mixture 20 min prior to fertilization. Eggs were then harvested by centrifugation, rinsed three times, re-suspended in Millipore-filtered sea water in the presence or absence of rapamycin and fertilized. At different times, batches (500 μl) of the embryo suspension were taken; the cells were pelleted and frozen in liquid nitrogen. Soluble protein extracts were prepared as described in Oulhen et al. (2010). [35S]- L-methionine incorporation into proteins was measured on duplicate aliquots of the extracts after 10% TCA precipitation on Whatman 3 M filters and counting in a scintillation counter in the presence of Optiphase Supermix scintillation liquid.
Measurement of 4E-BP Level After Fertilization
At different times, following fertilization, 20 μl pelleted embryos were dissolved in 150 μl of SDS-Fix buffer to prepare total crude protein extracts as above. Proteins were separated on SDS-PAGE and 4E-BP was analyzed by immunoblotting using the rabbit polyclonal antibodies directed against human 4E-BP (see above). The antigen-antibody complex was revealed by chemoluminescence, the signal was digitalized and quantified using the ImageJ 1.43j software. Densitometric analysis gave relative numerical values, which were used to calculate percentages of 4E-BP present in embryos considering as a reference (i.e., 100%) the quantity of total 4E-BP in unfertilized eggs. When necessary, percents were converted into absolute concentrations based on values for 4E-BP calculated as described above.
Surface Plasmon Resonance (SPR) Data Acquisition
Data were acquired with Reichert SR7000DC spectrometer instrument (Buffalo, USA). Running buffer was HEPES 50 mM pH 7.7 KCl 150 mM 0.5% Igepal and flow rate was 25 μL.min−1. 60 μRIU of Sp4E-BP was immobilized on a mixed SAM (1 C11-(OEG)6-COOH:10 C11-(OEG)3-OH) (Buffalo, USA) via classical amine coupling chemistry. For interaction experiment, concentration of Flag-MmIF4E1:m7GTP was assayed by spectrophotometry and stock solution was centrifuged before use. Then a concentration range from 4.37 μM to 53.9 nM of Flag-MmIF4E1:m7GTP (3-fold dilution series) was injected on the Sp4E-BP chip at 25°C. Following each Flag-MmIF4E1:m7GTP injection, the chip was regenerated with 30 s of 10 mM NaOH. Each curve displayed was double referenced with a set of 6 blank buffer injections. Data were then fitted using a global analysis method with Scrubber 2.0a software (Biologic Software, Australia). Fitting errors are reported for each kinetic constant.
The Minimal Model Elaboration
A model for cap-dependent translation initiation in sea urchin has been proposed (Belle et al., 2010) based on the systemic environment of BIOCHAM software (Calzone et al., 2006). The Biocham model contains too many reactions and undetermined parameters to allow quantitative simulations of dynamic changes occurring at fertilization. We therefore investigated the strategy of extracting a minimal core model. The model was constructed with the following reactions: (R1) the reversible association between eIF4E and 4E-BP, (R2) the reversible association of eIF4E to eIF4G, (R3) the irreversible production of protein by the initiation complex containing associated eIF4E:eIF4G, (R4) the synthesis of 4E-BP by the initiation complex containing associated eIF4E:eIF4G and (R5) the degradation of free 4E-BP.
(R1) eIF4E + 4E-BP <−> eIF4E:4E-BP (kon1/koff1 = 1/KD1)
(R2) eIF4E + eIF4G <−> eIF4E:eIF4G (kon2/koff2 = 1/KD2)
(R3) eIF4E:eIF4G -> Protein + eIF4E:eIF4G (kcatProtein)
(R4) eIF4E:eIF4G -> 4E-BP + eIF4E:eIF4G (kcat4E−BP)
(R5) 4E-BP -> 0 (klys4E−BP)
In this model, eIF4E is considered to be always associated to mRNAs since its affinity for the cap structure and the stability of the eIF4E: cap mRNA complex were demonstrated to be very high in other species (Sonenberg and Gingras, 1998). Global protein production is assumed to be proportional to the amount of eIF4E:eIF4G complex (R4). The total amounts of eIF4E and eIF4G were shown to remain constant after fertilization in sea urchin (Oulhen et al., 2007).
The differential equations resulting from the model using the law of mass action are as follow:
(1) d[eIF4E]/dt = koff1[eIF4E:4E-BP] + koff2[eIF4E:eIF4G] − kon1[eIF4E][4E-BP] − kon2[eIF4E][eIF4G]
(2) d[4E-BP]/dt = koff1[eIF4E:4E-BP] + kcat4E−BP[eIF4E:eIF4G] − klysis4E−BP[4E-BP] − kon1[eIF4E][4E-BP]
(3) d[eIF4G]/dt = koff2[eIF4E:eIF4G] − kon2[eIF4E][eIF4G]
(4) d[eIF4E:4E-BP]/dt = kon1[eIF4E][4E-BP] − koff1[eIF4E:4E-BP]
(5) d[eIF4E:eIF4G]/dt = kon2[eIF4E][eIF4G] − koff2[eIF4E:eIF4G]
(6) d[Protein]/dt = kcatProtein[eIF4E:eIF4G]
Parameter Determination in Unfertilized Eggs
The relevance of the core model in unfertilized eggs was studied by combining experimental data acquisition and learning procedure of unknown parameters. Initial total concentrations of 4E-BP, eIF4E, and eIF4G as well as kinetic parameters of the association of eIF4E:4E-BP and eIF4G:eIF4E complexes had to be experimentally determined and were further used to fit parameters for 4E-BP synthesis and degradation.
Concentrations of the translation factors
The concentrations of 4E-BP and eIF4E in unfertilized eggs were determined by immunoblotting and densitometric quantification as indicated in the Materials and Methods section. The total concentration of 4E-BP and eIF4E were respectively 3.67 μM (SD = 0.83, n = 4), and 2.15 μM (SD = 0.29, n = 6) thus indicating an excess of total 4E-BP over total eIF4E. The concentration of eIF4G could not be determined using the procedure described for 4E-BP and eIF4E for two reasons: first, sea urchin eIF4G was present under complex post-translational modified forms (Oulhen et al., 2007) and it was therefore not clear which bands could be ascribed to eIF4G, and second, no significant and measurable amounts of endogenous nor recombinant protein had been obtained. However, it was previously demonstrated that after fertilization a rapid exchange of 4E-BP for eIF4G occurred on eIF4E with a stoichiometric behavior (Oulhen et al., 2007). Therefore, simulations were performed using a minimal eIF4G concentration equivalent to eIF4E concentration 2.15 μM.
Determination of the kinetic parameters
The kinetic parameters for 4E-BP: eIF4E interaction were determined by surface plasmon resonance (SPR). 4E-BP was immobilized on a chip and several concentrations of eIF4E were injected onto the chip. Figure 1 shows the overlapped sensorgrams in relative Index of Refraction Unit (RIU) and their associated theoretical fits (solid lines) resulting from global data analysis according to a one step 1:1 reaction. The kinetic parameters determined were kon1 = 9.3 ± 0.1 × 103 M−1.s−1, koff1 = 2.2 ± 0.1 ×10−4 s−1 and the affinity constant KD1 = 23 nM (Table 1), all in good concordance with published data in other species (Abiko et al., 2007; Mizuno et al., 2008). Regarding the high conservation of the binding sites among species (Joshi et al., 2005) the measured parameters likely reflect the values in the sea urchin Sphaerechinus granularis.
Figure 1. Surface plasmon resonance Interaction of eIF4E and 4E-BP. Each sensorgram, relative refractive index (μRIU) as a function of time, was obtained after injection of eIF4E from 4.37 μM to 53.9 nM according to a three-fold dilution range. Overlapped signals were fitted using a single equilibrium kinetic isotherm (solid red lines).
A direct measurement of eIF4E:eIF4G interaction could not be analyzed by surface plasmon resonance. A kon2 = 1.82 × 103 M−1.s−1 and a koff2 = 2.0 × 10−4 s−1 for eIF4E:eIF4G interaction were used (Table 1) from published data in other species (Von Der Haar et al., 2006; Umenaga et al., 2011), resulting in determining an affinity constant for KD2 = 110 nM (Table 1).
Parameters for 4E-BP synthesis and degradation
The constants for the synthesis of 4E-BP (kcat4E−BP) and its degradation (klys4E−BP) had to be determined. The degradation constant of reaction R5 was calculated from experiments consisting in the measurement of the total amount of 4E-BP remaining after treatment of unfertilized eggs in the presence of the protein synthesis inhibitor emetine. In that condition, the disappearance of 4E-BP depends on reactions R1, R2, and R5 since no production could occur (R4). The disappearance of total 4E-BP was simulated and the best fitting value for klys4E−BP was determined by optimizing the least square error to the experimental values (Figure 2). The best fit was obtained for a value of 5.9 × 10−4 s−1 (Table 1).
Figure 2. Determination of the degradation rate of 4E-BP in unfertilized eggs. Unfertilized eggs were incubated in the presence of 100 μM emetine. The amount of total 4E-BP remaining upon time was determined from immunoblotting experiments as indicated in the material and methods section. The values from 8 independent experiments are plotted as black cross. Simulation using the model (reactions R1, R2, and R5) leading to the best fit is shown as red line. The total amount of 4E-BP is expressed as % of initial value.
Since 4E-BP remains constant in the untreated unfertilized egg, the synthesis constant, kcat4E−BP (R4), could be calculated using reactions R1 to R5. A least-square method was used to constraint a constant value, as close as possible to 100%, for the total amount of 4E-BP over time; the inferred catalytic constant for 4E-BP synthesis was kcat4E−BP = 3.2 × 10−3 s−1 (Table 1).
Calculation of factor concentrations
The unfertilized eggs are in a steady state in which the total concentrations of 4E-BP, eIF4E, and eIF4G remain constant as verified from immunoblotting experiments over the period of conservation of the eggs. Using reactions R1 and R2, the concentrations of free and complexed forms of 4E-BP, eIF4E, and eIF4G could be calculated. From the differential reactions (4) and (5) of the model:
(4) d[eIF4E:4E-BP]/dt = kon1[eIF4E][4E-BP] − koff1[eIF4E:4E-BP] = 0
(5) d[eIF4E:eIF4G]dt = kon2[eIF4E][eIF4G] − koff2[eIF4E:eIF4G] = 0,
and knowing the total concentrations:
[4E-BP]total = 3.67 μM, [eIF4E]total = [eIF4G]total = 2.15 μM, the two equations become:
kon1([eIF4E]total−[eIF4E:4E-BP]−[eIF4E:eIF4G]) ([4E-BP]total−[eIF4E:4E-BP])−koff1[eIF4E:4E-BP] = 0
kon2([eIF4E]total−[eIF4E:4E-BP]−[eIF4E:eIF4G]) ([eIF4G]total−[eIF4E:eIF4G])−koff2[eIF4E:eIF4G] = 0
from which the unknown values [eIF4E:4E-BP] and [eIF4E:eIF4G] can be calculated. From these, the free amounts of [eIF4E], [4E-BP],and [eIF4G]are deduced from the total amount minus the complexed forms
[eIF4E] = [eIF4E]total − [eIF4E:4E-BP]- [eIF4E:eIF4G]
[4E-BP] = [4E-BP] total − [eIF4E:4E-BP]
[eIF4G] = [eIF4G] total − [eIF4E:eIF4G]
The calculated values are all reported in Table 1.
At that point, the parameters and steady state concentrations of all the reactions of the model are known in the unfertilized eggs (Table 1). Since protein synthesis was determined by [35S]-methionine incorporation into all translated proteins and expressed as a percentage of incorporation, the kcatProtein could not be determined. However, since in the model, the amount of protein synthesized is proportional to the concentration of [eIF4E:eIF4G], changes in [eIF4E:eIF4G] will reflect the changes in the protein synthesis rates.
Learning Parameters and Concentrations After Fertilization
The kinetic of 4E-BP disappearance observed in vivo reveals two main features: a decrease of the total 4E-BP protein starting from fertilization and lasting about 15 min, followed by a stabilization of the level of the protein at 18–20% of the initial amount (Figure 3A). In parallel, the rate of protein synthesis increases and stabilizes around 30 min after fertilization resulting in the linear accumulation of neosynthetized proteins (Figure 3B). Therefore, fertilization corresponds to a time-dependent change from a steady state (unfertilized state) to another one (fertilized state) reached after 30 min. With respect to the minimal model proposed, two non-exclusive changes could explain the experimental values of total 4E-BP evolution: a destabilization of eIF4E:4E-BP complex (R1) and an increase in the degradation rate klysis4E−BP of 4E-BP. The two hypotheses were simulated separately. Since the biochemical modifications induced by fertilization are not instantaneous (Epel, 1990; Cormier et al., 2003; Gilbert, 2003), the time necessary for the biochemical modifications to occur at fertilization through the transduction pathway was introduced in the model as a parameter time change varying linearly from the initial value of parameters in unfertilized eggs to their final value after fertilization.
Figure 3. Changes in 4E-BP total amount and protein synthesis in vivo after fertilization. (A) 4E-BP was measured by immunoblotting and densitometric quantification as described in the material and methods section. Mean values from 10 independent experiments are plotted as a function of time. The total amount of 4E-BP is expressed as % of initial value. (B) Protein synthesis was determined from incorporation into proteins of [35S]methionine at different times after fertilization as indicated in the Materials and Methods section. Mean values of protein accumulation from 11 independent determinations are plotted as a function of time in arbitrary unit (UA).
A set of model simulations was first performed by perturbing the value koff1 (R1) ranging from its unfertilized egg value (Table 1) up to 100 times the value and by introducing a parameter time change ranging between 1 and 15 min. For each simulation, the mean-square error between the model prediction and 4E-BP experimental data was computed. The least-worst fit (sum of square residual = 46712) is shown in Figure 4A and, not surprisingly, the simulation led to a non-biologically relevant increase in the total amount of 4E-BP. A second set of simulations was performed by perturbing the value of klysis4E−BPranging from its value in unfertilized eggs (Table 1) to 100 times the value while still introducing a parameter time change ranging from 1 and 15 min. The least-worst fit (sum of square residual = 3122) is shown in Figure 4B and the corresponding simulation did not fully match the experimental data.
Figure 4. Simulation of fertilization changes on 4E-BP using the minimal model. Best fits (red curves) compared to experimental data (black cross) are shown: (A) Ranging koff1 from unfertilized value (1 to 100-fold) and the parameter time change (1 to 15 min). (B) Ranging klys4E-BP from unfertilized value (1–100-fold) and the parameter time change (1–15 min). (C) Changing koff1 and klys4E-BP from unfertilized value (1–100-fold) and the parameter time change (1–15 min). (D) Color representation of the fit according to the change of koff1 and klys4E-BP when the parameter time change is fixed to 5 min (kcat4E-BP and koff1 ratio are the ratio between the value reached after fertilization and the unfertilized value). The color scale on the right was associated to distance to data (sum of square residual) obtained in the simulations. The white cross corresponds to the best fit. The total amount of 4E-BP is expressed as % of initial value.
Therefore, simulations were performed by perturbing both the koff1 value (R1), and the klysis4E−BP (R4)values together with a parameter time change ranging from 1 to 15 min. Using least square method, a very good fit with the experimental data was obtained (sum of square residual = 1207). The best fit was obtained when koff1 increased 8-fold from the value in unfertilized eggs and klysis4E−BP increased 32.5-fold with a parameter time change of 5 min (Figure 4C). Figure 4D shows in a color scale the least square sum of square residual values obtained in the simulations for the ranging values of koff1 and klysis4E−BP. The best fit (white cross) was located in a small area related to the accuracy of the determinations.
An 8-fold increase in koff1 leads to a 8-fold increase in the KD1/KD2 ratio (R1 and R2), such an increase would also be obtained through a kon2 8-fold increase or a kon1 or koff2 8-fold decrease. We therefore performed simulations with these different parameters, associated with the 32.5-fold increase in klysis4E−BP and a parameter time change of 5 min. The best fits (sum of square residual) for each parameter were respectively 1207 for koff1, 3692 for kon2, 3419 for kon1 and 4214 for koff2 (Figure 5) indicating that only the 8-fold increase in koff1 led to a good sum of square residual value.
Figure 5. Simulations performed varying the indicated parameters (koff1, kon2, kon1, koff2) at fertilization. klys4E-BP was fixed as 32.5-fold increase compared to unfertilized eggs. Parameter time change from unfertilized values was set at 5 min. The best fits (red line) for each parameter are shown (A–D) as compared to experimental data (black cross). The total amount of 4E-BP is expressed as % of initial value.
Figure 6 shows the distance to data (sum of square residual value and SD) calculated when each parameter varied by steps from 1/32 to 32-fold compared to unfertilized values. Simulation with the best fit (sum of square residual = 1189) was obtained with respectively a koff1 × 8, kon1 × 1, and kon2 and koff2 × 1/27. Regarding that this sum of square residual is not significantly different from the distance (1207) obtained with the unique koff1 variation, the koff1 role in eIF4E:4E-BP destabilization at fertilization appears to be the most relevant.
Figure 6. Distance to data calculations from simulations performed varying together all of the parameters koff1, kon2, kon1, koff2. Each indicated parameter varied by steps from 1/32 to 32-fold compared to unfertilized values in such a way that a 8-fold increase in KD1/KD2 was maintained constant (this gives 10434 possibilities). The klys4E-BP was fixed as 32.5-fold increase compared to unfertilized eggs and the parameter time change was fixed to 5 min. For each possible ratio (fertilized value vs. unfertilized value), the mean and SD of the distances to data were reported. Green bar corresponds to region where simulation fits well.
Altogether, the model allows to conclude that destabilization of eIF4E:4E-BP complex by an 8-fold increase of its dissociation rate (koff1) associated with a 32.5-fold increase in the mechanism of 4E-BP degradation, both taking place in 5 min following fertilization, are necessary and sufficient to explain the observed experimental changes occurring in the total amount of 4E-BP.
Using the minimal model, the parameters determined in the unfertilized eggs and the changes predicted in the fertilized eggs (Table 1) the kinetic of concentration changes of all parameters were simulated (Figure 7). Importantly, the concentration of eIF4E:eIF4G complex increased 4.2-fold from its initial value in the unfertilized eggs, in a time interval of 30 min. The resulting calculated increase and the protein accumulation kinetic (Figure 7, black line) were highly compatible with the in vivo protein synthesis changes after fertilization (see Figure 3B). The 4.2-fold increase in eIF4E:eIF4G complex compares favorably with the experimental 6.6-fold increase in protein synthesis measure as described in the material and methods section (SD = 2.3, n = 8 independent experiments). Although we are aware that reaction R4 of the model is a very highly simplified representation of the mechanism of cap-dependent translation, and that many other changes on translation factors have been reported at fertilization in sea urchin (Oulhen et al., 2007; Belle et al., 2011; Costache et al., 2012) the assumption that translation was proportional to the amount of eIF4E:eIF4G complex appeared rational in the minimal model elaboration.
Figure 7. Simulation of the concentration changes occurring at fertilization. The concentration changes of the indicated constituents were calculated and plotted vs. time after fertilization using the parameters depicted in Table 1; “eIF4E,” “eIF4G,” and “4E-BP” are the concentrations of the free forms of these proteins; “eIF4E:eIF4G” and “eIF4E:4E-BP” correspond to the concentrations of the complexed forms; “Total 4E-BP” represents the total concentration of 4E-BP (free and complexed). Normalization was done with 100% corresponding to the initial concentration of total 4E-BP, i.e., 3.67 μM (see Table 1). The kinetic of accumulation of protein was calculated from the kinetic of eIF4E:4E-BP changes and is shown in relative units (black curve).
Simulation of Rapamycin Effect on 4E-BP Changes and Protein Synthesis Increase at Fertilization
It has been already reported that FRAP/mTOR is involved in the protein synthesis increase following fertilization (Salaun et al., 2003, 2004, 2005; Oulhen et al., 2007). The effect of rapamycin, an inhibitor of the protein kinase FRAP/mTOR, has been analyzed in early development of sea urchin embryo. Protein synthesis measured as described in the Materials and Methods section was 53.0% (SD = 5.1, n = 8) of the value in control fertilized eggs. Rapamycin slowed down the decrease in the total amount 4E-BP, which stabilized at 41.7% (SD = 5.4, n = 8) of unfertilized value after 30 min. This observation was rather surprising since this value is higher than the value in fertilized eggs (18%), while protein synthesis was decreased as compared to control fertilized eggs.
The changes observed in the presence of rapamycin were modeled as a modification of the parameters of the minimal model. Simulations were performed with koff1 ranging from 1 to 8-fold, klysis4E−BP from 1 to 32.5-fold and changes for time parameter from 1 to 100 min. A good fit was obtained for a koff1 1-fold the value in unfertilized eggs, klysis4E−BP value 16-fold the unfertilized value and a parameter time change of 33 min (Figure 8 and Table 1). In these conditions, the concentration of eIF4E:eIF4G at steady state was 2.58-fold compared to the 4.25-fold in fertilized eggs in the absence of rapamycin. The model therefore predicts a rapamycin-induced limited increase in protein synthesis of 53.6%, in very good concordance with the experimental data value of 52.0%.
Figure 8. Simulation of fertilization changes on 4E-BP in the presence of rapamycin. Simulated curve (red line) obtained with optimized parameters (see text) compared to experimental data (black cross). The total amount of 4E-BP is expressed as % of initial value.
Therefore the model predicted that not only koff1 increase after fertilization but also the degradation mechanism of 4E-BP is under the control of FRAP/mTOR pathway.
While the regulation of protein synthesis is a highly complex process, we show here that using a minimal model based on three main actors eIF4E, eIF4G, and 4E-BP, the changes occurring at fertilization in sea urchin could be fully simulated with adjustment of a minimum of parameters. The model postulates that fertilization triggers two events: (1) an increase in the KD1 of the eIF4E:4E-BP complex due to an 8-fold increase of the dissociation constant (koff1) and (2) a 32.5-fold increase in the mechanism of 4E-BP degradation (klysis4E−BP). Therefore, the disappearance of 4E-BP cannot result from the unique dissociation of the eIF4E:4E-BP complex. Interestingly, in mammalian cells it has been found that 4E-BP1 is degraded in eIF4E-knock-down cells (Yanagiya et al., 2012). The authors show that the non-eIF4E-bound hyperphosphorylated 4E-BP1 is degraded while eIF4E-bound hypophosphorylated 4E-BP1 is stable reinforcing our assumption in the model that only free 4E-BP is degraded. However, it should be noted that there is no indication in this study of an activation of the mechanism of degradation itself while it is the case in sea urchin fertilization.
As expected, the model predicts that rapamycin reverses the fertilization-induced change in koff1. An attractive hypothesis would be that fertilization in sea urchin activates the kinase FRAP/mTOR that phosphorylates 4E-BP and increases the koff1 of its association with eIF4E, resulting in a destabilization of eIF4E:4E-BP complex. A role for FRAP/mTOR protein kinase on the destabilization of eIF4E:4E-BP complex at fertilization is compatible with the observations (1) that rapamycin, a FRAP/mTOR inhibitor, inhibits protein synthesis increase in sea urchin fertilization, and inhibits the disappearance of 4E-BP upon fertilization and (2) that the 4E-BP protein was shown to be a substrate for FRAP/mTOR in other organisms; in mammals, multiple and hierarchical phosphorylation events provoke the release of 4E-BP1 from eIF4E, in concordance with a KD1 increase (Gingras et al., 2001). Although the relationship between FRAP/mTOR activity and the affinity of 4E-BP for eIF4E is not established in sea urchin embryos (Oulhen et al., 2009), it has been shown that the degradation of 4E-BP correlates with its phosphorylation (Cormier et al., 2001). Therefore, the possibility that fertilization activates FRAP/mTOR resulting in a koff1 increase should be experimentally analyzed when the purified proteins will be available under their non-phosphorylated and phosphorylated forms.
We cannot exclude that fertilization could have also modified KD2. Since eIF4G undergoes electrophoretic changes at fertilization (Oulhen et al., 2007) it would be of interest to identify the post-translational changes occurring on this factor, and its consequences on eIF4E:eIF4G interaction.
Furthermore and unexpectedly, the model also predicts that the mechanism of 4E-BP degradation was also rapamycin-sensitive. Very interestingly, the degradation mechanism of 4E-BP appears to be a FRAP/mTOR target, which could also be biochemically tested when the mechanism will be identified. For now, this feature is described for the first time using sea urchin fertilization and would be of great interest to be searched in other species and other physiological regulations.
Sébastien Laurent and Adrien Richard equally contributed to the article as first author. Sébastien Laurent and Didier Flament designed and realized the SPR experiments. Virginie Glippa determined 4E-BP concentration in sea urchin eggs. Pauline Gosselin prepared and provided the proteins for SPR determinations. Odile Mulner-Lorillon, Julia Morales, Patrick Cormier provided the biochemical data on translation activity and protein accumulation. and Robert Bellé designed and coordinated the study. All authors contributed to the model elaboration and to the analysis of the results.
Conflict of Interest Statement
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. The Guest Associate Editor Thierry Tonon declares that, despite being affiliated to the same institution as authors Patrick Cormier, Virginie Glippa, Julia Morales and Odile Mulner-Lorillon, the review process was handled objectively and no conflict of interest exists.
This work was supported by the Ligue contre le cancer (Western France regional branch, Finistère, Côtes d'Armor, Deux Sèvres, Morbihan, and Vendée committees), the Brittany Regional Council and the Finistère Departmental Council. This work was also partially support by the French Agency for Research (ANR-10_BLANC-0218 BioTempo project) and by PEPS QuantOursins CNRS grant.
Abiko, F., Tomoo, K., Mizuno, A., Morino, S., Imataka, H., and Ishida, T. (2007). Binding preference of eIF4E for 4E-binding protein isoform and function of eIF4E N-terminal flexible region for interaction, studied by SPR analysis. Biochem. Biophys. Res. Commun. 355, 667–672. doi: 10.1016/j.bbrc.2007.01.198
Belle, R., Pluchon, P. F., Cormier, P., and Mulner-Lorillon, O. (2011). Identification of a new isoform of eEF2 whose phosphorylation is required for completion of cell division in sea urchin embryos. Dev. Biol. 350, 476–483. doi: 10.1016/j.ydbio.2010.12.015
Belle, R., Prigent, S., Siegel, A., and Cormier, P. (2010). Model of cap-dependent translation initiation in sea urchin: a step towards the eukaryotic translation regulation network. Mol. Reprod. Dev. 77, 257–264. doi: 10.1002/mrd.21142
Brunn, G. J., Fadden, P., Haystead, T. A., and Lawrence, J. C. Jr. (1997). The mammalian target of rapamycin phosphorylates sites having a (Ser/Thr)-Pro motif and is activated by antibodies to a region near its COOH terminus. J. Biol. Chem. 272, 32547–32550. doi: 10.1074/jbc.272.51.32547
Burnett, P. E., Barrow, R. K., Cohen, N. A., Snyder, S. H., and Sabatini, D. M. (1998). RAFT1 phosphorylation of the translational regulators p70 S6 kinase and 4E-BP1. Proc. Natl. Acad. Sci. U.S.A. 95, 1432–1437. doi: 10.1073/pnas.95.4.1432
Calzone, L., Fages, F., and Soliman, S. (2006). BIOCHAM: an environment for modeling biological systems and formalizing experimental knowledge. Bioinformatics 22, 1805–1807. doi: 10.1093/bioinformatics/btl172
Cormier, P., Pyronnet, S., Morales, J., Mulner-Lorillon, O., Sonenberg, N., and Belle, R. (2001). eIF4E association with 4E-BP decreases rapidly following fertilization in sea urchin. Dev. Biol. 232, 275–283. doi: 10.1006/dbio.2001.0206
Costache, V., Bilotto, S., Laguerre, L., Belle, R., Cosson, B., Cormier, P., et al. (2012). Dephosphorylation of eIF2alpha is essential for protein synthesis increase and cell cycle progression after sea urchin fertilization. Dev. Biol. 365, 303–309. doi: 10.1016/j.ydbio.2012.03.002
Gingras, A. C., Gygi, S. P., Raught, B., Polakiewicz, R. D., Abraham, R. T., Hoekstra, M. F., et al. (1999b). Regulation of 4E-BP1 phosphorylation: a novel two-step mechanism. Genes Dev. 13, 1422–1437. doi: 10.1101/gad.13.11.1422
Gingras, A. C., Raught, B., and Sonenberg, N. (1999a). eIF4 initiation factors: effectors of mRNA recruitment to ribosomes and regulators of translation. Annu. Rev. Biochem. 68, 913–963. doi: 10.1146/annurev.biochem.68.1.913
Gosselin, P., Oulhen, N., Jam, M., Ronzca, J., Cormier, P., Czjzek, M., et al. (2011). The translational repressor 4E-BP called to order by eIF4E: new structural insights by SAXS. Nucleic Acids Res. 39, 3496–3503. doi: 10.1093/nar/gkq1306
Haghighat, A., Mader, S., Pause, A., and Sonenberg, N. (1995). Repression of cap-dependent translation by 4E-binding protein 1: competition with p220 for binding to eukaryotic initiation factor-4E. EMBO J. 14, 5701–5709.
Jackson, R. J., Hellen, C. U., and Pestova, T. V. (2010). The mechanism of eukaryotic translation initiation and principles of its regulation. Nat. Rev. Mol. Cell Biol. 11, 113–127. doi: 10.1038/nrm2838
Jackson, R. J., Hellen, C. U., and Pestova, T. V. (2012). Termination and post-termination events in eukaryotic translation. Adv. Protein Chem. Struct. Biol. 86, 45–93. doi: 10.1016/B978-0-12-386497-0.00002-5
Jackson, R. J., Hunt, S. L., Reynolds, J. E., and Kaminski, A. (1995). Cap-dependent and cap-independent translation: operational distinctions and mechanistic interpretations. Curr. Top. Microbiol. Immunol. 203, 1–29. doi: 10.1007/978-3-642-79663-0_1
Mader, S., Lee, H., Pause, A., and Sonenberg, N. (1995). The translation initiation factor eIF-4E binds to a common motif shared by the translation factor eIF-4 gamma and the translational repressors 4E-binding proteins. Mol. Cell. Biol. 15, 4990–4997.
Mathews, M. B., Sonenberg, N., and Hershey, J. W. B. (2007). “Origins and principles of translational control,” in Translational Control in Biology and Medecine, eds N. Sonenberg, J. Hershey, and M. Mathews (New York, NY: CSHL Press), 1–31.
Mizuno, A., In, Y., Fujita, Y., Abiko, F., Miyagawa, H., Kitamura, K., et al. (2008). Importance of C-terminal flexible region of 4E-binding protein in binding with eukaryotic initiation factor 4E. FEBS Lett. 582, 3439–3444. doi: 10.1016/j.febslet.2008.09.003
Morales, J., Mulner-Lorillon, O., Cosson, B., Morin, E., Belle, R., Bradham, C. A., et al. (2006). Translational control genes in the sea urchin genome. Dev. Biol. 300, 293–307. doi: 10.1016/j.ydbio.2006.07.036
Oulhen, N., Boulben, S., Bidinosti, M., Morales, J., Cormier, P., and Cosson, B. (2009). A variant mimicking hyperphosphorylated 4E-BP inhibits protein synthesis in a sea urchin cell-free, cap-dependent translation system. PLoS ONE 4:e5070. doi: 10.1371/journal.pone.0005070
Oulhen, N., Mulner-Lorillon, O., and Cormier, P. (2010). eIF4E-binding proteins are differentially modified after ammonia versus intracellular calcium activation of sea urchin unfertilized eggs. Mol. Reprod. Dev. 77, 83–91. doi: 10.1002/mrd.21110
Oulhen, N., Salaun, P., Cosson, B., Cormier, P., and Morales, J. (2007). After fertilization of sea urchin eggs, eIF4G is post-translationally modified and associated with the cap-binding protein eIF4E. J. Cell Sci. 120, 425–434. doi: 10.1242/jcs.03339
Pause, A., Belsham, G. J., Gingras, A. C., Donze, O., Lin, T. A., Lawrence, J. C., et al. (1994). Insulin-dependent stimulation of protein synthesis by phosphorylation of a regulator of 5'-cap function. Nature 371, 762–767. doi: 10.1038/371762a0
Pyronnet, S., Imataka, H., Gingras, A. C., Fukunaga, R., Hunter, T., and Sonenberg, N. (1999). Human eukaryotic translation initiation factor 4G (eIF4G) recruits mnk1 to phosphorylate eIF4E. EMBO J. 18, 270–279. doi: 10.1093/emboj/18.1.270
Salaun, P., Boulben, S., Mulner-Lorillon, O., Belle, R., Sonenberg, N., Morales, J., et al. (2005). Embryonic-stage-dependent changes in the level of eIF4E-binding proteins during early development of sea urchin embryos. J. Cell Sci. 118, 1385–1394. doi: 10.1242/jcs.01716
Salaun, P., Le Breton, M., Morales, J., Belle, R., Boulben, S., Mulner-Lorillon, O., et al. (2004). Signal transduction pathways that contribute to CDK1/cyclin B activation during the first mitotic division in sea urchin embryos. Exp. Cell Res. 296, 347–357. doi: 10.1016/j.yexcr.2004.02.013
Salaun, P., Pyronnet, S., Morales, J., Mulner-Lorillon, O., Belle, R., Sonenberg, N., et al. (2003). eIF4E/4E-BP dissociation and 4E-BP degradation in the first mitotic division of the sea urchin embryo. Dev. Biol. 255, 428–439. doi: 10.1016/S0012-1606(02)00099-4
Umenaga, Y., Paku, K. S., In, Y., Ishida, T., and Tomoo, K. (2011). Identification and function of the second eIF4E-binding region in N-terminal domain of eIF4G: comparison with eIF4E-binding protein. Biochem. Biophys. Res. Commun. 414, 462–467. doi: 10.1016/j.bbrc.2011.09.084
Von Der Haar, T., Oku, Y., Ptushkina, M., Moerke, N., Wagner, G., Gross, J. D., et al. (2006). Folding transitions during assembly of the eukaryotic mRNA cap-binding complex. J. Mol. Biol. 356, 982–992. doi: 10.1016/j.jmb.2005.12.034
Keywords: translational control, sea urchin embryos, mechanisms of fertilization, deterministic model, translation simulation
Citation: Laurent S, Richard A, Mulner-Lorillon O, Morales J, Flament D, Glippa V, Bourdon J, Gosselin P, Siegel A, Cormier P and Bellé R (2014) Modelization of the regulation of protein synthesis following fertilization in sea urchin shows requirement of two processes: a destabilization of eIF4E:4E-BP complex and a great stimulation of the 4E-BP-degradation mechanism, both rapamycin-sensitive. Front. Genet. 5:117. doi: 10.3389/fgene.2014.00117
Received: 24 February 2014; Accepted: 16 April 2014;
Published online: 06 May 2014.
Edited by:Thierry Tonon, CNRS-UPMC, France
Copyright © 2014 Laurent, Richard, Mulner-Lorillon, Morales, Flament, Glippa, Bourdon, Gosselin, Siegel, Cormier and Bellé. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Robert Bellé, Integrative Biology of Marine Models, Translation Cell Cycle and Development, Sorbonne Universités, CNRS-UPMC University Paris 06, Station Biologique, place Georges Teissier, CS 90074, 29688 Roscoff Cedex, France e-mail: email@example.com