Corrigendum: Enhancement of RNA-directed DNA methylation of a transgene by simultaneously downregulating a ROS1 ortholog using a virus vector in Nicotiana benthamiana
- Research Faculty of Agriculture, Hokkaido University, Sapporo, Japan
by Otagaki, S., Kasai, M., Masuta, C. and Kanazawa, A. (2013). Front. Genet. 4:44. doi: 10.3389/fgene.2013.00044
We have become aware that an incorrect image was mistakenly used for Figure 2C in the original publication. The correct version of Figure 2 is shown here. We should also note that the primers used for quantitative RT-PCR of the NbROS1 gene listed in Table A1 turned out to have a sequence mismatch with the target. The mismatched nucleotides in these primers are underlined: 5′-CCAAGAAGCTGGTAGGTTAT-3′ (NbROS1 real 3′ F); 5′-GCAAACACCTCGTTTAACTT-3′ (NbROS1 real 3′ R). We found that a set of primers without sequence mismatch (5′-CCAAGAAGCTGGTAGGCTAT-3′; 5′-GCAAACACCTCGTTAACTT-3′) yielded amplification products at a higher level, although both primer sets amplified a single DNA fragment and were valid for quantification, i.e., comparison of the relative level of NbROS1 mRNA between samples.
Figure 2. Gene silencing using the CMV-A1 vector. (A) Schematic representation of the vector constructs targeting the CaMV 35S promoter or the NbROS1 coding sequence. (B) Changes in mRNA level of NbROS1 as a consequence of infection with virus that contains the NbROS1 insert (A1:NbROS1). The NbROS1 mRNA level was assessed relative to the actin mRNA level in leaf tissues at 18 days post-inoculation (DPI). Data are the means and standard errors obtained from three replicates. Both the control and A1:NbROS1-infected plants were infected with A1:35Spro to eliminate nonspecific effects of viral infection on the mRNA level of NbROS1. (C) Northern blot analysis of low-molecular weight RNAs isolated from leaf tissues of plants infected with A1:NbROS1 and the control virus that lacked an insert at 14 DPI, probed for the NbROS1 gene. Ethidium-bromide-stained 5S rRNA and tRNAs bands are shown below the panel to show that an equal amount of the small RNA fraction was loaded.
The correct Figure 2 with its legend appears below. The authors apologize for any inconvenience caused.
Conflict of Interest Statement
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Keywords: Cucumber mosaic virus, DNA demethylation, RNA-directed DNA methylation, ROS1, virus-induced gene silencing
Citation: Otagaki S, Kasai M, Masuta C and Kanazawa A (2017) Corrigendum: Enhancement of RNA-directed DNA methylation of a transgene by simultaneously downregulating a ROS1 ortholog using a virus vector in Nicotiana benthamiana. Front. Genet. 8:5. doi: 10.3389/fgene.2017.00005
Received: 02 December 2016; Accepted: 13 January 2017;
Published: 01 February 2017.
Edited and reviewed by: Michael Wassenegger, RLP AgroScience, Germany
Copyright © 2017 Otagaki, Kasai, Masuta and Kanazawa. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Akira Kanazawa, email@example.com