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Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Genet. | doi: 10.3389/fgene.2018.00414

Gga-miR-205a affecting myoblast proliferation and differentiation by targeting CDH11

 Zhijun Wang1,  Hongjia Ouyang2, Xiaolan Chen1,  Jiao Yu1, Biao Chen1 and  Qinghua NIE1*
  • 1South China Agricultural University, China
  • 2Zhongkai University of Agriculture and Engineering, China

Non-coding RNAs especially miRNAs have found to play important roles during skeletal muscle development. Our previous RNA-Seq performed on breast muscle tissue from 7 weeks old Recessive White Rock and Xinhua Chicken and leg muscle tissue from female Xinghua Chicken at three development time point (11 embryo age, 16 embryo age, and 1day post hatch) (accession number GSE62971 and GSE89355, respectively) showed that miR-205a and CDH11 were differentially expressed genes. In this study, we found that miR-205a was sharply upregulated at 11 embryo age (E11) and reached another peak at E15 after a continuous decline. The overexpression of miR-205a could inhibit both Quail muscle clone 7 cell lines (QM7) and chicken primary myoblast (CPM) proliferation, at the meantime promote the differentiation of myoblasts by directly binding to the 3’UTR of CDH11. Conversely, Overexpression of CDH11 significantly facilitated QM7 and CPM proliferation and hampered CPM differentiation.
The Dual-Luciferase Reporter Assay results showed that MyoG and HNF-1 could possibly regulate the expression of miR-205a by binding to the active region of miR-205a. Altogether our data suggest that MyoG could possibly stimulate miR-205a expression to suppress CDH11, which promotes myoblasts proliferation while represses the differentiation.

Keywords: miR-205a, CDH11, Myoblast, myogenesis, promoter active region

Received: 20 Jun 2018; Accepted: 05 Sep 2018.

Edited by:

Hans Cheng, Agricultural Research Service (USDA), United States

Reviewed by:

Shaojun Liu, Hunan Normal University, China
Jie Mei, Huazhong Agricultural University, China  

Copyright: © 2018 Wang, Ouyang, Chen, Yu, Chen and NIE. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Prof. Qinghua NIE, South China Agricultural University, Guangzhou, 510642, Guangdong Province, China, nqinghua@scau.edu.cn