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Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Genet. | doi: 10.3389/fgene.2018.00617

Nuclear accumulation of HSP70 in mouse skeletal muscles in response to heat stress, aging, and unloading with or without reloading

  • 1Centre of Human and Applied Physiological Sciences, Department of Basic and Clinical Neuroscience, Institute of Psychiatry, Psychology & Neuroscience, King's College London, United Kingdom
  • 2Department of Physiology, Graduate School of Health Sciences, Toyohashi Sozo University, Japan
  • 3Biological Sciences, Graduate School of Sciences and Technology for Innovation, Yamaguchi University, Japan

The purpose of this study was to investigate the nuclear accumulation of heat shock protein 70 (HSP70), a molecular chaperonin in mouse skeletal muscle in response to aging, heat stress, and hindlimb unloading with or without reloading. Profiles of HSP70-specific nuclear transporter Hikeshi in skeletal muscles were also evaluated. Heat stress-associated nuclear accumulation of HSP70 was observed in slow soleus (SOL) and fast plantaris (PLA) muscles of young (10-week old) mice. Nuclear accumulation level of HSP70 in slow medial gastrocnemius (MGAS) of aged (100-week old) mice was significantly higher than that of young (10-week old) mice (p<0.05). Reloading following 2-week hindlimb unloading caused accumulation of HSP70 in myonuclei in MGAS and PLA of young mice (p<0.05). However, reloading-associated nuclear accumulation of HSP70 was not observed in both types of muscles of aged mice. On the other hand, 2-week hindlimb unloading had no impact on the nuclear accumulation of HSP70 in both muscles of young and aged mice. Nuclear expression level of Hikeshi in both MGAS and PLA in mice was suppressed by aging. No significant changes in the nuclear Hikeshi in both muscles was induced by unloading with or without reloading. Results of this study indicate that the nuclear accumulation of HSP70 might show a protective response against cellular stresses in skeletal muscle, and that the protective response may be suppressed by aging. Protective response to aging might be depend on muscle fiber types.

Keywords: skeletal muscle, Hsp70, Hikeshi, Aging, unloading, Reloading, Heat stress

Received: 30 Apr 2018; Accepted: 23 Nov 2018.

Edited by:

Richard C. Siow, King's College London, United Kingdom

Reviewed by:

Carsten Merkwirth, Ferring Research Institute, Inc., United States
Mark A. McCormick, University of New Mexico, United States  

Copyright: © 2018 Apostolopoulos, Nakamura, Yokoyama, Aoshima, Fujimoto, Nakamura, Ito and Goto. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Prof. Katsumasa Goto, Graduate School of Health Sciences, Toyohashi Sozo University, Department of Physiology, Toyohashi, 4408511, Aichi, Japan, gotok@sepia.ocn.ne.jp