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Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Genet. | doi: 10.3389/fgene.2018.00630

Population Genetic Diversity and Phylogenetic Characteristics for High-Altitude Adaptive Kham Tibetan Revealed by Dnatyper™ 19 Amplification System

 Yiping Hou1*, Xing Zou1,  Zheng Wang1,  Guanglin He1, Mengge Wang1, Yongdong Su2, Jing Liu1, Pengyu Chen3, Shouyu Wang1, Bo Gao1 and Zhao Li1
  • 1West China College of Basic and Forensic Medicine, Sichuan University, China
  • 2Center for Forensic Identification, Department of Tibet Autonomous Region Public Security, China
  • 3School of Forensic Medicine, Zunyi Medical University, China

Tibetans residing in the high-altitude inhospitable environment have undergone significant natural selection of their genetic architecture. Recently, highly mutational autosomal short tandem repeats were widely used not only in the anthropology and population genetics to investigate the genetic structure and relationships, but also in the medical genetics to explore the pathogenesis of multiple genetic diseases and in the forensic science to identify individual and parentage relatedness. However, genetic variants and forensic efficiency of DNATyper™ 19 amplification system and genetic background of Kham Tibetan remain uncharacterized. Thus, we genotyped 19 forensic genetic markers in 11,402 Kham Tibetans to gain insight into the genetic diversity of Chinese high-altitude adaptive population. Highly discriminating and polymorphic forensic measures were observed, which indicated that this new-developed DNATyper™ 19 PCR amplification is suitable for routine forensic identification purposes and Chinese national DNA database establishment. Pairwise genetic distances among the comprehensive population comparisons suggested that this high-altitude adaptive Kham Tibetan has genetically closer relationships with lowlanders of Tibeto-Burman-speaking populations (Chengdu Tibetan, Liangshan Tibetan and Liangshan Yi). Genetic substructure analyses via phylogenetic reconstruction, principal component analysis and multidimensional scaling analysis in both nationwide and worldwide contexts suggested that the genetic proximity exists along the linguistic, ethnic and continental geographical boundary. Further studies with whole-genome sequencing of modern or archaic Kham Tibetans would be useful in reconstructing the Tibetan population history.

Keywords: Forensic Genetics, Population relationship, Short tandem repeat (STR), Genetic polymorphism, Tibetan

Received: 21 Sep 2018; Accepted: 26 Nov 2018.

Edited by:

Fulvio Cruciani, Sapienza University of Rome, Italy

Reviewed by:

Antonio González-Martín, Complutense University of Madrid, Spain
CHUANCHAO WANG, Xiamen University, China  

Copyright: © 2018 Hou, Zou, Wang, He, Wang, Su, Liu, Chen, Wang, Gao and Li. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Prof. Yiping Hou, West China College of Basic and Forensic Medicine, Sichuan University, Chengdu, China, forensic@scu.edu.cn