Original Research ARTICLE
CeRNA Expression Profiling Identifies KIT Related circRNAs-miRNA-mRNA Networks in Gastrointestinal Stromal Tumors
- 1Anhui Medical University, China
- 2Fudan University, China
- 3Okayama University, Japan
- 4Shanghai Public Health Clinical Center, Fudan University, China
Gastrointestinal stromal tumors (GISTs) are the most common human sarcomas and typically locate in the stomach or small intestine. Although circular RNAs (circRNAs) were identified to plays vital roles in tumor oncogenesis and progression, the molecular basis for aggressive tumor biology of these circRNAs in GISTs remains unclear. In this study, we applied SBC ceRNA microarrays to screen for tumor-specifc circRNA profiles in GISTs and identified that a total of 5770 circRNAs and 1815 mRNAs were differentially expressed in GISTs. Three significantly differential circRNAs (circ_0069766, circ_0084097, and circ_0079471) and their host genes (KIT, PLAT, and ETV1) were also verified in 68 pairs of GIST and adjacent normal gastric tissues by qRT-PCR. GIST-specific circRNA-miRNA-mRNA regulatory networks analysis demonstrated that the specific KIT related regulatory networks were involved in the three circRNAs, the circRNA host genes and three miRNAs (miR-142-5p, miR-144-3p and miR-485-3p), which may be key regulators of GISTs and served as molecular biomarkers and potential therapeutic targets for this malignant disease.
Keywords: CircRNAs, KIT, Plat, ETV1, Regulatory networks analysis, GISTs (gastrointestinal stromal tumors)
Received: 13 Dec 2018;
Accepted: 09 Aug 2019.
Copyright: © 2019 Jia, Tong, Yong, Katayama, Wang, Lu, Zhang and Wang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Prof. Jin Wang, Shanghai Public Health Clinical Center, Fudan University, Shanghai, China, firstname.lastname@example.org