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Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Genet. | doi: 10.3389/fgene.2019.00908

Comprehensive RNA-seq data analysis identifies key mRNAs and lncRNAs in atrial fibrillation

Dong-Mei Wu1, Shao-Hua Fan1, Zi-Hui Zheng2, Xin Wen1, Xin-Rui Han1, Shan Wang1, Yong-Jian Wang1, Zi-Feng Zhang1, Qun Shan1, Meng-Qiu Li1, Bin Hu1, Jun Lu1, Gui-Quan Chen3 and  Yuan-Lin Zheng4*
  • 1School of Life Science, Jiangsu Normal University, China
  • 2School of Medicine and Life Sciences, Nanjing University of Chinese Medicine, China
  • 3Model Animal Research Center, Nanjing University, China
  • 4Jiangsu Normal University, China

Long non-coding RNAs (LncRNAs) are an emerging class of RNA species that may play a critical regulatory role in gene expression. However, the association between lncRNAs and atrial fibrillation (AF) is still not fully understood. In this study, we used RNA sequencing data to identify and quantify the both protein coding genes (PCGs) and lncRNAs. The high enrichment of these up-regulated genes in biological functions concerning response to virus and inflammatory response suggested that chronic viral infection may lead to activated inflammatory pathways, thereby alter the electrophysiology, structure, and autonomic remodeling of the atria. In contrast, the downregulated GO terms were related to the response to saccharides. To identify key lncRNAs involved in AF, we predicted lncRNAs regulating expression of the adjacent PCGs, and characterized biological function of the dysregulated lncRNAs. We found that two lncRNAs, ETF1P2 and AP001053.11, could interact with protein-coding genes (PCGs), which were implicated in AF. In conclusion, we identified key PCGs and lncRNAs, which may be implicated in AF, which not only improves our understanding of the roles of lncRNAs in AF, but also provides potentially functional lncRNAs for AF researchers.

Keywords: long non-coding RNAs, Atrial Fibrillation, RNA-Seq, Genes, Protein coding genes

Received: 12 May 2019; Accepted: 28 Aug 2019.

Copyright: © 2019 Wu, Fan, Zheng, Wen, Han, Wang, Wang, Zhang, Shan, Li, Hu, Lu, Chen and Zheng. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Mx. Yuan-Lin Zheng, Jiangsu Normal University, Xuzhou, China, zhuotong01566@163.com