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Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Genet. | doi: 10.3389/fgene.2019.00946

Single Nucleotide Polymorphisms (SNPs) in starch biosynthetic genes associated with increased resistant starch concentration in rice mutant

  • 1Tamil Nadu Agricultural University, India
  • 2University of Saskatchewan, Canada
  • 3DRDO-BU Center for Life Sciences, India
  • 4University of Mysore, India
  • 5King Saud University, Saudi Arabia

Abstract
Resistant Starch (RS), plays a crucial role in human health and nutrition by controlling glucose metabolism. RS or dietary fibre content in rice is low because it goes through a variety of process before it is ready for cooking and consumption. Hence, this study was carried out to develop a rice mutant with increased RS. The rice mutant (γ278) with increased RS was developed by utilizing gamma (γ) rays as a mutagen. Mutant γ278 was characterized for mutations in the starch biosynthetic genes viz., GBSSI, SSI, SSIIa, SSIIIa, SBEIa and SBEIIb to reveal the functional mutations/variations led to high RS content in rice. A total of 31 sequence variants/mutations in six genes were identified. We report the discovery of three deleterious mutation/variants each in GBSSI, SSIIa, and SSIIIa with the potential to increase RS content in rice. Further, wild x mutant crosses were made to develop an F2 population to study the effect of combination of deleterious mutations. The SNP (GBSSI:ssIIa:ssIIIa) combination responsible for high RS content in F2 population was identified and recorded highest amylose content (AC) (26.18%) and RS (8.68%) content. In conclusion, this marker combination will be highly useful to develop a rice variety with increased RS.

Keywords: Amylose, Resistant Starch, Starch biosynthesis, Rice-, Mutation/variants

Received: 25 Jan 2019; Accepted: 05 Sep 2019.

Copyright: © 2019 Gurunathan, Ramadoss, M, Nayak, Kalagatur, Bapu, Mohan, Alqarawi, Hashem and Abd_Allah. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Dr. Selvakumar Gurunathan, Tamil Nadu Agricultural University, Coimbatore, 641 003, Tamil Nadu, India, selvakumaragri@gmail.com